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Tytuł pozycji:

Increased AOC1 Expression Promotes Cancer Progression in Colorectal Cancer

Tytuł:
Increased AOC1 Expression Promotes Cancer Progression in Colorectal Cancer
Autorzy:
Fangyuan Liu
Weijun Ou
Wenbo Tang
Zhenyu Huang
Zhehui Zhu
Wenjun Ding
Jihong Fu
Yilian Zhu
Chenying Liu
Weimin Xu
Peng Du
Temat:
AOC1
colorectal cancer
proliferation
migration
organoid
EMT
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RC254-282
Źródło:
Frontiers in Oncology, Vol 11 (2021)
Wydawca:
Frontiers Media S.A., 2021.
Rok publikacji:
2021
Kolekcja:
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
2234-943X
Relacje:
https://www.frontiersin.org/articles/10.3389/fonc.2021.657210/full; https://doaj.org/toc/2234-943X
DOI:
10.3389/fonc.2021.657210
Dostęp URL:
https://doaj.org/article/32626ff56f69490dbbac14cb28a7cd20  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.32626ff56f69490dbbac14cb28a7cd20
Czasopismo naukowe
BackgroundAmine oxidase copper containing 1 (AOC1) is a gene whose biological function in colorectal cancer (CRC) has not been elucidated. Therefore, the purpose of this study was to investigate the clinical significance of AOC1 expression in CRC and its biological function in CRC cell lines.Materials and MethodsAOC1 expression levels were examined in paired CRC and peritumoral tissues, and distant liver metastatic tissues were examined using quantitative real-time PCR, western blotting, and immunohistochemistry staining. The log-rank test and Cox regression model were used to analyze the relationship between AOC1 expression and prognosis. Proliferation assays (Cell Counting Kit‐8 and colony formation assays), migration assays (Transwell and wound healing assays) and xenograft tumor formation in nude mice were performed to assess the biological role of AOC1 in CRC cells.ResultsAOC1 expression significantly increased in human CRC tissues, especially in liver metastases, and was associated with a worse prognosis. In addition, AOC1 had higher expression in tumor organoids than in normal organoids, suggesting that it was highly expressed in the tumor epithelium. Functional analysis demonstrated that AOC1 knockdown inhibited the proliferation and migration of CRC cells by inducing EMT in vitro. Xenograft tumor formation in nude mice showed that knockdown of AOC1 inhibited the tumor xenografts growth in vivo.ConclusionHigh expression of AOC1 was significantly associated with worse clinical outcomes, was an independent risk factor for poor prognosis, and promoted aggressive CRC cell phenotypes. AOC1 is expected to become a novel biomarker for predicting the prognosis of patients with CRC and an effective therapeutic target in clinical practice.

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