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Tytuł pozycji:

Carnitine/organic cation transporter 1 precipitates the progression of interstitial fibrosis through oxidative stress in diabetic nephropathy in mice

Tytuł:
Carnitine/organic cation transporter 1 precipitates the progression of interstitial fibrosis through oxidative stress in diabetic nephropathy in mice
Autorzy:
Shohei Makiishi
Kengo Furuichi
Yuta Yamamura
Keisuke Sako
Yasuyuki Shinozaki
Tadashi Toyama
Shinji Kitajima
Yasunori Iwata
Norihiko Sakai
Miho Shimizu
Tomoko Hirose-Sugiura
Shuichi Kaneko
Yukio Kato
Takashi Wada
Temat:
Medicine
Science
Źródło:
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Wydawca:
Nature Portfolio, 2021.
Rok publikacji:
2021
Kolekcja:
LCC:Medicine
LCC:Science
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
2045-2322
Relacje:
https://doaj.org/toc/2045-2322
DOI:
10.1038/s41598-021-88724-4
Dostęp URL:
https://doaj.org/article/33ff7b36717046dea11ba4198bc3f989  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.33ff7b36717046dea11ba4198bc3f989
Czasopismo naukowe
Abstract Carnitine/organic cation transporter 1 (OCTN1) is the only known uptake transporter for ergothioneine which is a food-derived strong antioxidant amino acid that is absorbed by OCTN1. We previously reported the roles of OCTN1/ergothioneine in the progression of kidney fibrosis in ischemic kidney disease. In this study, we evaluated the roles of OCTN1 in the progression of diabetic kidney disease. A diabetic kidney disease model was induced in octn1 knockout and wild-type mice by streptozotocin (STZ). Oxidative stress, represented by urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG), were higher in the octn1 knockout mice. Azan- and Sirius red-positive areas increased significantly in the octn1 knockout mice. Gene expression was evaluated by cluster analysis, and shown to be different in the octn1 knockout mice compared with the wild-type mice. In a pathway analysis, the pathway associated with the cytoskeleton and cell adhesion increased. In accordance with interstitial fibrosis in octn1 knockout mice, gene expression of moesin in the injured kidney, known as an associated protein of cytoskeleton and cell membranes, was doubled 28 weeks after STZ injection. In addition, the moesin protein was expressed in a part of α-SMA-positive renal tubular epithelial cells. These findings were confirmed by cultured murine proximal tubular epithelial cells: The expression of moesin was induced under oxidative stress with hydrogen peroxide. These data indicate that OCTN1 would play some roles in progression of interstitial fibrosis under oxidative stress via moesin expression in diabetic kidney disease.
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