Dynamic Transcriptome-Proteome Correlation Networks Reveal Human Myeloid Differentiation and Neutrophil-Specific Programming

Summary: Human neutrophilic granulocytes form the largest pool of innate immune cells for host defense against bacterial and fungal pathogens. The dynamic changes that accompany the metamorphosis from a proliferating myeloid progenitor cell in the bone marrow into a mature non-dividing polymorphonuclear blood cell have remained poorly defined. Using mass spectrometry-based quantitative proteomics combined with transcriptomic data, we report on the dynamic changes of five developmental stages in the bone marrow and blood. Integration of transcriptomes and proteome unveils highly dynamic and differential interactions between RNA and protein kinetics during human neutrophil development, which can be linked to functional maturation of typical end-stage blood neutrophil killing activities. : Human neutrophils form the largest pool of innate immune cells. Using mass spectrometry-based quantitative proteomics combined with transcriptomics, Hoogendijk et al. report on the dynamic changes of five developmental stages, unveiling highly dynamic RNA and protein kinetics that can be linked to functional maturation of end-stage blood neutrophils. Keywords: neutrophil development, granule proteins, proteomics, transcriptomics, granulopoiesis