The first serious outbreak of Flavescence dorée (FD) in Piedmont (North-West Italy) dates back to 1998 in the Tortona area (Alessandria province). FD is a serious quarantine-worthy disease transmitted by the leafhopper Scaphoideus titanus Ball. Different Vitis vinifera L. biotypes react differently to the phytoplasma, in particular as to the accumulation of polyphenols in leaves. In this experimentation, we observed and described concentration and accumulation of the main classes of polyphenols in entire leaves and in leaf blades and veins of two varieties, Nebbiolo and Barbera, displaying different levels of susceptibility to FD. Their well-known different reactions could be related, at least partially, to leaf polyphenols, both as to concentrations and profiles. Nebbiolo displayed some specific traits: i) the higher percentage of incidence over totals of individual molecules known to be powerful antioxidants (caftaric acid over coutaric acid; quercetin glycosides over other flavonols); ii) the higher concentration of flavanols in veins with respect to Barbera and their wider profile (astilbin and a taxifolin-glycoside, this last accumulating exclusively in Nebbiolo).