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Tytuł pozycji:

miR-497-5p Inhibits Proliferation of Pancreatic Cancer Cells by Targeting CCNE1 Gene

Tytuł :
miR-497-5p Inhibits Proliferation of Pancreatic Cancer Cells by Targeting CCNE1 Gene
Autorzy :
JIANG Dawei
XU Liu
WANG Weilin
Pokaż więcej
Temat :
mir-497-5p
paca
prognosis
proliferation
ccne1
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RC254-282
Źródło :
Zhongliu Fangzhi Yanjiu, Vol 47, Iss 8, Pp 600-606 (2020)
Wydawca :
Magazine House of Cancer Research on Prevention and Treatment, 2020.
Rok publikacji :
2020
Kolekcja :
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Typ dokumentu :
article
Opis pliku :
electronic resource
Język :
Chinese
ISSN :
1000-8578
Relacje :
http://html.rhhz.net/ZLFZYJ/html/8578.2020.19.1596.htm; https://doaj.org/toc/1000-8578
DOI :
10.3971/j.issn.1000-8578.2020.19.1596
Dostęp URL :
https://doaj.org/article/5ba220cb4fd345a1ae8272d54ccf3cdb
Numer akcesji :
edsdoj.5ba220cb4fd345a1ae8272d54ccf3cdb
Czasopismo naukowe
Objective To determine miR-497-5p expression in pancreatic cancer (PaCa) and its clinical significance, and to explore its effects and mechanisms on the proliferation of PaCa cells. Methods The expression levels of miR-497-5p were detected by real-time quantitative PCR experiment. The relation between miR-497-5p expression and clinicopathological features, the prognosis of PaCa patients were analyzed by Chi-square test and Kaplan-Meier method. The effects of miR-497-5p overexpression on the proliferation and cell cycle of Capan-2 and PANC-1 cells were detected by cell counting kit-8 (CCK-8) experiment and flow cytometry. The relation between miR-497-5p level and the cyclin E1 (CCNE1) mRNA expression was analyzed by Spearman correlation test. The regulatory effect of miR-497-5p on CCNE1 expression was confirmed by dual luciferase reporter experiment and Western blot. Results miR-497-5p expression in cancer tissues was significantly lower than that in adjacent normal tissues (P < 0.001), and its expression in T3+T4 stages cancer tissues were significantly lower than those in T1+T2 stages cancer tissues (P < 0.001); low expression of miR-497-5p was associated with advanced T stage (P=0.003); The 5-year overall survival rate of patients with low miR-497-5p expression was significantly lower than those with high miR-497-5p expression (P=0.036). Compared with the control group, miR-497-5p overexpression significantly reduced cell proliferation, increased the cell proportion in G0/G1 phase and decreased the cell proportion in S phase. The expression levels of CCNE1 mRNA in cancer tissues were significantly higher than those in adjacent normal tissues (P < 0.001), and its expression was negatively correlated with miR-497-5p expression (P < 0.001). miR-497-5p could bind directly to the 3'-UTR of CCNE1 mRNA and inhibit the expression of CCNE1 protein. Conclusion miR-497-5p expression is downregulated in PaCa cells and associated with relatively advanced T stage and poor prognosis; the overexpression of miR-497-5p induces the cell cycle arrest to inhibit the proliferation of PaCa cells by targeting CCNE1 gene.

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