Expression loss and revivification of RhoB gene in ovary carcinoma carcinogenesis and development.

Szczegóły
Opis

Tytuł:: Expression loss and revivification of RhoB gene in ovary carcinoma carcinogenesis and development.
Autorzy:: Yingwei Liu
Na Song
Kexing Ren
Shenglan Meng
Yao Xie
Qida Long
Xiancheng Chen
Xia Zhao
Temat:: Medicine
Science
Źródło:: PLoS ONE, Vol 8, Iss 11, p e78417 (2013)
Wydawca:: Public Library of Science (PLoS), 2013.
Rok publikacji:: 2013
Kolekcja:: LCC:Medicine
LCC:Science
Typ dokumentu:: article
Opis pliku:: electronic resource
Język:: English
ISSN:: 1932-6203
Relacje:: http://europepmc.org/articles/PMC3815309?pdf=render; https://doaj.org/toc/1932-6203
DOI:: 10.1371/journal.pone.0078417
Dostęp URL:: https://doaj.org/article/5ba4db0c45fb41689fc62729dd527e33 Link otwiera się w nowym oknie
Numer akcesji:: edsdoj.5ba4db0c45fb41689fc62729dd527e33
: Czasopismo naukowe

Pełny tekst

RhoB, a member of small GTPases belonging to the Ras protein superfamily, might have a suppressive activity in cancer progression. Here, expression of RhoB gene was evaluated in human benign, borderline and malignant ovary tumors by immunostaining, with normal ovary tissue as control. Malignant tumors were assessed according to Federation Internationale de Gynecologie Obstetrique (FIGO) guidelines and classified in stage I-IV. Revivification of RhoB gene was investigated by analyzing the effect of histone deacetylase (HDAC) inhibitor trichostatin (TSA) and methyltransferase inhibitor 5-azacytidine (5-Aza) on ovarian cancer cells via RT-PCR and western blot. Apoptosis of ovary cancer cells was detected using flowcytometry and fluorescence microscopy. Subsequently, RhoB expression is detected in normal ovary epithelium, borderline tumors, and decreases significantly or lost in the majority of ovarian cancer specimen (P

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