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Tytuł pozycji:

Use of resuscitation promoting factors to screen for tuberculosis infection in household-exposed children in The Gambia

Tytuł:
Use of resuscitation promoting factors to screen for tuberculosis infection in household-exposed children in The Gambia
Autorzy:
W. van Loon
M. P. Gomez
D. Jobe
K. L. M. C. Franken
T. H. M. Ottenhoff
M. Coninx
L. Kestens
J. S. Sutherland
B. Kampmann
L. D. Tientcheu
Temat:
Pediatric tuberculosis
IGRA
RpfB
RpfD
Immunology
Diagnostics
Infectious and parasitic diseases
RC109-216
Źródło:
BMC Infectious Diseases, Vol 20, Iss 1, Pp 1-9 (2020)
Wydawca:
BMC, 2020.
Rok publikacji:
2020
Kolekcja:
LCC:Infectious and parasitic diseases
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
1471-2334
Relacje:
http://link.springer.com/article/10.1186/s12879-020-05194-1; https://doaj.org/toc/1471-2334
DOI:
10.1186/s12879-020-05194-1
Dostęp URL:
https://doaj.org/article/62c957adf37148d096e558e1bb4b2d3d  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.62c957adf37148d096e558e1bb4b2d3d
Czasopismo naukowe
Abstract Background Interferon-γ release assays (IGRA) with Resuscitation promoting factor (Rpf) proteins enhanced tuberculosis (TB) screening and diagnosis in adults but have not been evaluated in children. Children often develop paucibacillary TB and their immune response differs from that of adults, which together affect TB disease diagnostics and immunodiagnostics. We assessed the ability of Rpf to identify infection among household TB-exposed children in The Gambia and investigated their ability to discriminate Mycobacterium tuberculosis complex (MTBC) infection from active TB disease in children. Methods Detailed clinical investigations were done on 93 household TB-exposed Gambian children and a tuberculin skin test (TST) was administered to asymptomatic children. Venous blood was collected for overnight stimulation with ESAT-6/CFP-10-fusion protein (EC), purified protein derivative and RpfA, B, C, D and E. Interferon gamma (IFN-γ) production was measured by ELISA in supernatants and corrected for the background level. Infection status was defined by IGRA with EC and TB disease by mycobacterial confirmation and/or clinical diagnosis. We compared IFN-γ levels between infected and uninfected children and between infected and TB diseased children using a binomial logistic regression model while correcting for age and sex. A Receiver Operating Characteristics analysis was done to find the best cut-off for IFN-γ level and calculate sensitivity and specificity. Results Interferon gamma production was significantly higher in infected (IGRA+, n = 45) than in uninfected (IGRA-, n = 20) children after stimulation with RpfA, B, C, and D (P = 0.03; 0.007; 0.03 and 0.003, respectively). Using RpfB and D-specific IFN-γ cut-offs (33.9 pg/mL and 67.0 pg/mL), infection was classified with a sensitivity-specificity combination of 73–92% and 77–72% respectively, which was similar to and better than 65–75% for TST. Moreover, IFN-γ production was higher in infected than in TB diseased children (n = 28, 5 bacteriologically confirmed, 23 clinically diagnosed), following RpfB and D stimulation (P = 0.02 and 0.03, respectively). Conclusion RpfB and RpfD show promising results for childhood MTBC infection screening, and both performed similar to and better than the TST in our study population. Additionally, both antigens appear to discriminate between infection and disease in children and thus warrant further investigation as screening and diagnostic antigens for childhood TB.
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