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Tytuł pozycji:

Circular RNA circDNM3OS Functions as a miR-145-5p Sponge to Accelerate Cholangiocarcinoma Growth and Glutamine Metabolism by Upregulating MORC2

Tytuł:
Circular RNA circDNM3OS Functions as a miR-145-5p Sponge to Accelerate Cholangiocarcinoma Growth and Glutamine Metabolism by Upregulating MORC2
Autorzy:
Su Y
Yu T
Wang Y
Huang X
Wei X
Temat:
cca
circdnm3os
mir-145-5p
morc2
glutamine
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RC254-282
Źródło:
OncoTargets and Therapy, Vol Volume 14, Pp 1117-1129 (2021)
Wydawca:
Dove Medical Press, 2021.
Rok publikacji:
2021
Kolekcja:
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
1178-6930
Relacje:
https://www.dovepress.com/circular-rna-circdnm3os-functions-as-a-mir-145-5p-sponge-to-accelerate-peer-reviewed-article-OTT; https://doaj.org/toc/1178-6930
Dostęp URL:
https://doaj.org/article/657a8e0c58c04637b4b0f43269b6ba96  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.657a8e0c58c04637b4b0f43269b6ba96
Czasopismo naukowe
Yongfeng Su,1 Ting Yu,1 Yaqi Wang,1 Xianming Huang,2 Xiaoyong Wei3 1Department of General Oncology, Jiangxi Provincial Cancer Hospital, Nanchang, Jiangxi, 330029, People’s Republic of China; 2Department of Pathology, Jiangxi Provincial Cancer Hospital, Jiangxi, 330029, People’s Republic of China; 3Department of Hepatobiliary Surgery, Jiangxi Provincial Cancer Hospital, Jiangxi, 330029, People’s Republic of ChinaCorrespondence: Xiaoyong WeiDepartment of Hepatobiliary Surgery, Jiangxi Provincial Cancer Hospital, No. 519 Beijing East Road, Qingshanhu District, Nanchang, Jiangxi, People’s Republic of ChinaTel +86 791-88326292Email DrSu1982@163.comBackground: Cholangiocarcinoma (CCA) is the second most common liver malignant tumor. CircRNA hsa_circ_0005230 (circDNM3OS) has been reported to exert an oncogenic role in CCA. However, the mechanisms related to circDNM3OS in CAA progression have not been fully elucidated.Methods: The expression of circDNM3OS, microRNA (miR)-145-5p, and MORC2 (MORC Family CW-Type Zinc Finger 2) mRNA were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation, colony formation, migration, invasion, and apoptosis were evaluated by Cell Counting Kit-8 (CCK-8), colony formation, transwell, wound-healing, and flow cytometry assays. The levels of glutamine, α-KG (α-ketoglutarate), and ATP (adenosine triphosphate) were detected using commercial kits. The relationship between circDNM3OS or MORC2 and miR-145-5p was verified by dual-luciferase reporter and/or RNA immunoprecipitation (RIP) assays. Protein level of MORC2 was measured by Western blotting. The role of circDNM3OS in CCA growth was verified by xenograft experiment.Results: CircDNM3OS and MORC2 were upregulated while miR-145-5p was downregulated in CCA tissues and cells. Inhibition of circDNM3OS reduced xenograft tumor growth in vivo and constrained proliferation, colony formation, migration, invasion, induced apoptosis, and reduced glutamine metabolism of CCA cells in vitro. CircDNM3OS sponged miR-145-5p to elevate MORC2 expression. MiR-145-5p silencing overturned circDNM3OS knockdown-mediated influence on malignancy and glutamine metabolism of CCA cells. Also, MORC2 overexpression reversed the repressive impact of miR-145-5p mimic on malignancy and glutamine metabolism of CCA cells.Conclusion: CircDNM3OS facilitates CCA growth and glutamine metabolism by regulating the miR-145-5p/MORC2 pathway, offering a novel mechanism to understand the progression of CCA.Keywords: CCA, circDNM3OS, miR-145-5p, MORC2, glutamine
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