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Tytuł pozycji:

Epidemiological and Genomic Characterization of Campylobacter jejuni Isolates from a Foodborne Outbreak at Hangzhou, China

Tytuł :
Epidemiological and Genomic Characterization of Campylobacter jejuni Isolates from a Foodborne Outbreak at Hangzhou, China
Autorzy :
Hua Yu
Mohammed Elbediwi
Xiaohong Zhou
Huiqun Shuai
Xiuqin Lou
Haoqiu Wang
Yan Li
Min Yue
Pokaż więcej
Temat :
Campylobacter jejuni
foodborne outbreak
genomic investigation
pulse field gel electrophoresis
ST2988
Biology (General)
QH301-705.5
Chemistry
QD1-999
Źródło :
International Journal of Molecular Sciences, Vol 21, Iss 3001, p 3001 (2020)
Wydawca :
MDPI AG, 2020.
Rok publikacji :
2020
Kolekcja :
LCC:Biology (General)
LCC:Chemistry
Typ dokumentu :
article
Opis pliku :
electronic resource
Język :
English
ISSN :
1422-0067
1661-6596
Relacje :
https://www.mdpi.com/1422-0067/21/8/3001; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067
DOI :
10.3390/ijms21083001
Dostęp URL :
https://doaj.org/article/e68bb082957f4fd49a74a91cb4e5acff
Numer akcesji :
edsdoj.68bb082957f4fd49a74a91cb4e5acff
Czasopismo naukowe
Background: Foodborne outbreaks caused by Campylobacter jejuni have become a significant public health problem worldwide. Applying genomic sequencing as a routine part of foodborne outbreak investigation remains in its infancy in China. We applied both traditional PFGE profiling and genomic investigation to understand the cause of a foodborne outbreak in Hangzhou in December 2018. Method: A total of 43 fecal samples, including 27 sick patients and 16 canteen employees from a high school in Hangzhou city in Zhejiang province, were recruited. Routine real-time fluorescent PCR assays were used for scanning the potential infectious agents, including viral pathogens (norovirus, rotavirus, adenovirus, and astrovirus), and bacterial pathogens (Salmonella, Shigella, Campylobacter jejuni, Vibrio parahaemolyticus and Vibrio cholerae). Bacterial selection medium was used to isolate and identify the positive bacteria identified by molecular test. Pulsed field gel electrophoresis (PFGE), and next generation sequencing (NGS) were applied to fifteen recovered C. jejuni isolates to further understand the case linkage of this particular outbreak. Additionally, we retrieved reference genomes from the NCBI database and performed a comparative genomics analysis with the examined genomes produced in this study. Results: The analyzed samples were found to be negative for the queried viruses. Additionally, Salmonella, Shigella, Vibrio parahaemolyticus and Vibrio cholera were not detected. Fifteen C. jejuni strains were identified by the real-time PCR assay and bacterial selection medium. These C. jejuni strains were classified into two genetic profiles defined by the PFGE. Out of fifteen C. jejuni strains, fourteen have a unified consistent genotype belonging to ST2988, and the other strain belongs to ST8149, with a 66.7% similarity in comparison with the rest of the strains. Moreover, all fifteen strains harbored blaOXA-61 and tet(O), in addition to a chromosomal mutation in gyrA (T86I). The examined fourteen strains of ST2988 from CC354 clone group have very minimal genetic difference (3~66 SNPs), demonstrated by the phylogenomic investigation. Conclusion: Both genomic investigation and PFGE profiling confirmed that C. jejuni ST2988, a new derivative from CC354, was responsible for the foodborne outbreak Illustrated in this study.

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