In order to investigate the effects of different maternal corm weight and different levels of cow manure on saffron (Crocus sativus L.) production, an experiment was conducted at the Agricultural Research Station, Ferdowsi University of Mashhad during 2011- 2012 and 2012- 2013 growing seasons. For this purpose a factorial experiment was used based on complete randomized block design with three replications and 16 treatments. The experimental treatments were done at 4 levels of maternal corm weight (1.1- 3, 3.1- 5, 5.1- 7 and 7.1- 9 g) and 4 levels of cow manure (0, 20, 40 and 60 t.ha-1). Variance analysis results for studied characteristics of saffron corm showed that maternal corm weight, cow manure and maternal corm weight × cow manure had significant effects on these characteristics. Among the experimental treatments, maternal corm with 7.1- 9 g weight and the use rate of 60 t.ha-1 of cow manure treatment had both the highest total corm number (510 corm.m-2) and corm yield (1044 g.m-2). It seems that the corms with higher weight in the first year produce larger number of replacement corm than the corms with lower weight. The saffron flower yield characteristics showed that flower yield in the first year increased by increasing the maternal corm weight. The results of variance analysis of the number of flowers and fresh and dry yield of flower and stigma of saffron in the second year showed that the maternal corm weight, cow manure and maternal corm weight × cow manure had significant effects on them. These characteristics increased by increasing the maternal corm weight and levels of cow manure. It seems that producing replacement corm with high weight in the first year, requires large amount of maternal corm and high levels of cow manure usage.