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Tytuł pozycji:

Сlinical signs and pathoanatomical changes in the brain for poisoning of domestic turkeys with diazinon

Tytuł:
Сlinical signs and pathoanatomical changes in the brain for poisoning of domestic turkeys with diazinon
Autorzy:
R. S. Dankovych
V. V. Tumanov
Temat:
diazinon, poisoning, domestic turkey, acetylcholinesterase, pathmorphology, brain, synapses, hyperemia, necrosis
Veterinary medicine
SF600-1100
Źródło:
Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки, Vol 20, Iss 92, Pp 222-228 (2018)
Wydawca:
Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, 2018.
Rok publikacji:
2018
Kolekcja:
LCC:Veterinary medicine
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
Russian
Ukrainian
ISSN:
2518-7554
2518-1327
Relacje:
https://nvlvet.com.ua/index.php/journal/article/view/1781; https://doaj.org/toc/2518-7554; https://doaj.org/toc/2518-1327
DOI:
10.32718/nvlvet9246
Dostęp URL:
https://doaj.org/article/6db55303cb984f01aa75558c834f2750  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.6db55303cb984f01aa75558c834f2750
Czasopismo naukowe
For today, the actual problem of veterinary medicine is the poisoning of animals with organophosphorus pesticides, which are used as insecticides and acaricides, fungicides, herbicides, rodenticides, nematocides. One of the highly effective nonspecific organophosphorus insecticides is diazinon, which enters the body causing poisoning of various species of birds. The basis of the toxic action of diazinon is the ability to block the enzyme acetylcholinesterase. As a result, acetylcholine is accumulated in cholinergic synapses, excessive stimulation of the nerves and muscles is noted, and in the future, a violation of the normal passage of nerve impulses develops. Research was carried out of domestic turkeys (Meleagris gallopavo) in which spontaneous poisoning with diazinon was recorded. Detailed study of clinical signs that develop as a result of poisoning. The activity of the acetylcholinesterase of the serum of blood in the experimental birds was determined by the method of Hestrin. The section of birds that were killed was carried out by the method of Shore. Sections of organs were fixed in 10% neutral formalin, 96% ethanol and Carnoua liquid. The histo-cuttings were made by means of a stent microtome, stained with hematoxylin and eosin. The histological study of the preparations and their photographing was performed using a Leica DM-2500 (Switzerland) microscope, a Leica DFC450C camera and the Leica Application Suite Version 4.4 software. For the production of ultrathin sections, as well as for conducting electron microscopy, pieces of organs were fixed in Millonig’s buffer pH 7,36. Half-thin and ultrathin cuts were made on ultramicrotome LKB-2188 Ultrotome NOVA. The specimens were viewed and photographed in a Tesla BS-500 transmissive electron microscope, at an accelerating voltage of 60, 90 kV. In the pathoanatomical study, expressed discirculatory processes in the form of acute congestive hyperemia, stasis, perivascular edema and hemorrhages, as well as necrotic changes in cardiomyces, hepatocytes, nephrocytes and brain neurons. An electron microscopic study also found destruction of synapses in the cerebral cortex, cerebellum and medulla oblongata. When autopsy selected material (feed the masses crop of birds) for chemical-toxicological research. As a result the research the extract of selected content crop of birds by the method of thin-layer chromatography paper manifestation of iodine bismuth quality received positive reaction on the compound diazinon.

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