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Tytuł pozycji:

Detection of EGFR Mutations in cfDNA and CTCs, and Comparison to Tumor Tissue in Non-Small-Cell-Lung-Cancer (NSCLC) Patients

Tytuł :
Detection of EGFR Mutations in cfDNA and CTCs, and Comparison to Tumor Tissue in Non-Small-Cell-Lung-Cancer (NSCLC) Patients
Autorzy :
Haiyan E. Liu
Meghah Vuppalapaty
Charles Wilkerson
Corinne Renier
Michael Chiu
Clementine Lemaire
James Che
Melissa Matsumoto
James Carroll
Steve Crouse
Violet R. Hanft
Stefanie S. Jeffrey
Dino Di Carlo
Edward B. Garon
Jonathan Goldman
Elodie Sollier
Pokaż więcej
Temat :
Vortex technology
circulating tumor cell
total liquid biopsy
epidermal grow factor receptor
EGFR mutation analysis
Non-small cell carcinoma
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RC254-282
Źródło :
Frontiers in Oncology, Vol 10 (2020)
Wydawca :
Frontiers Media S.A., 2020.
Rok publikacji :
2020
Kolekcja :
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Typ dokumentu :
article
Opis pliku :
electronic resource
Język :
English
ISSN :
2234-943X
Relacje :
https://www.frontiersin.org/article/10.3389/fonc.2020.572895/full; https://doaj.org/toc/2234-943X
DOI :
10.3389/fonc.2020.572895
Dostęp URL :
https://doaj.org/article/723a9ab238364e0eb7b4e993350af58a
Numer akcesji :
edsdoj.723a9ab238364e0eb7b4e993350af58a
Czasopismo naukowe
Lung cancer is the leading cause of cancer-related mortality worldwide. Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) therapies, based on the evaluation of EGFR mutations, have shown dramatic clinical benefits. EGFR mutation assays are mainly performed on tumor biopsies, which carry risks, are not always successful and give results relevant to the timepoint of the assay. To detect secondary EGFR mutations, which cause resistance to 1st and 2nd generation TKIs and lead to the administration of a 3rd generation drug, effective and non-invasive monitoring of EGFR mutation status is needed. Liquid biopsy analytes, such as circulating tumor cells (CTCs) and circulating tumor DNA (cfDNA), allow such monitoring over the course of the therapy. The aim of this study was to develop and optimize a workflow for the evaluation of cfDNA and CTCs in NSCLC patients all from one blood sample. Using Vortex technology and EntroGen ctEGFR assay, EGFR mutations were identified at 0.5 ng of DNA (∼83 cells), with a sensitivity ranging from 0.1 to 2.0% for a total DNA varying from 25 ng (∼4 CTCs among 4000 white blood cells, WBCs) to 1 ng (∼4 CTCs among 200 WBCs). The processing of plasma-depleted-blood provided comparable capture recovery as whole blood, confirming the possibility of a multimodality liquid biopsy analysis (cfDNA and CTC DNA) from a single tube of blood. Different anticoagulants were evaluated and compared in terms of respective performance. Blood samples from 24 NSCLC patients and 6 age-matched healthy donors were analyzed with this combined workflow to minimize blood volume needed and sample-to-sample bias, and the EGFR mutation profile detected from CTCs and cfDNA was compared to matched tumor tissues. Despite the limited size of the patient cohort, results from this non-invasive EGFR mutation analysis are encouraging and this combined workflow represents a valuable means for informing therapy selection and for monitoring treatment of patients with NSCLC.

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