Preparation, Biocompatibility and Antitumor Activity of Nanodelivery System Targeting Breast Cancer Base on a Silica Nanoparticle

Jiuzhou Liu,1,* Shasha Ren,2,* Xiangyu Zhang,3 Yun Feng,2 Zhenglun Qiu,2 Li Ma,4 Jingwen Huang4 1Department of Breast and Thyroid Surgery, The First Affiliated Hospital of Henan University of Science and Technology, Luoyang, 471003, People’s Republic of China; 2Department of Breast and Thyroid Surgery, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang, 471009, People’s Republic of China; 3Department of Pathology, Jining First People’s Hospital, Jining Medical University, Jining, 272000, People’s Republic of China; 4The First Affiliated Hospital of Bengbu Medical College, Bengbu, 233004, People’s Republic of China*These authors contributed equally to this workCorrespondence: Jingwen Huang; Jiuzhou Liu Email byyfyhjw1988@163.com; Liu_JiuzhouBC@163.comBackground: Breast cancer (BC) is the most common type of cancer among women worldwide, and about 30% of males will have recurrent disease.Methods: In order to treat recurrent BC, we designed a type of silica nanodelivery system loaded with epirubicin and curcumin (composite nanoparticles, CNPs). To promote CNPs clinical application, the stability, the blood, immune and cell compatibility, skin stimulation experiments, anti-tumor activity in vivo and in vitro were studied.Results: In our study, the CNPs had a particle size of 73.9 nm and a uniform size and morphology; moreover, they maintained physical and chemical stability in the blood protein environment. Additionally, results showed that nanoparticles had good blood and immune compatibility, and they did not affect intracellular superoxide dismutase (SOD) and intracellular catalase (CAT). Skin stimulation experiments showed that CNPs did not cause any obvious irritative damage to the intact skin of rabbits. In the cytotoxicity study, CNPs showed strongest antitumor activity. The results of cell cycle and apoptosis studies showed that CNPs could mainly induce apoptosis of S and G2/M phase cells. In vivo, CNPs showed strongest aggregation in the tumor after 6 h of tail vein administration, and a large amount of CNPs continued to accumulate in the blood after 12 h of administration, indicating that CNPs had long circulation ability. The in vivo antitumor activities showed that CNPs had the strongest antitumor activity and tumor targeting ability, and hematoxylin-eosin staining of internal organs showed no obvious difference between treatment groups and negative control.Conclusion: CNPs have an ideal biosafety and therapeutic effect for recurrent BC, and they have potential clinical application value.Keywords: nanoparticles, breast cancer, chemotherapy, targeting, biocompatibility