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Tytuł pozycji:

DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis

Tytuł :
DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis
Autorzy :
Ma C
Ma N
Qin L
Miao C
Luo M
Liu S
Pokaż więcej
Temat :
dicer1-as1
mir-296-5p
crc
proliferation
metastasis
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RC254-282
Źródło :
Cancer Management and Research, Vol Volume 12, Pp 10035-10046 (2020)
Wydawca :
Dove Medical Press, 2020.
Rok publikacji :
2020
Kolekcja :
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Typ dokumentu :
article
Opis pliku :
electronic resource
Język :
English
ISSN :
1179-1322
Relacje :
https://www.dovepress.com/dicer1-as1-promotes-the-malignant-behaviors-of-colorectal-cancer-cells-peer-reviewed-article-CMAR; https://doaj.org/toc/1179-1322
Dostęp URL :
https://doaj.org/article/c8819dcb0c00459a960c18a4cab0837f
Numer akcesji :
edsdoj.8819dcb0c00459a960c18a4cab0837f
Czasopismo naukowe
Chuanyu Ma,1 Ning Ma,1 Lili Qin,1 Chuanna Miao,1 Minglei Luo,1 Shuhong Liu2 1Department of Proctology, Linyi Central Hospital, Linyi, Shandong Province, People’s Republic of China; 2Department of Radiotherapy, Linyi Cancer Hospital, Linyi, Shandong Province, People’s Republic of ChinaCorrespondence: Shuhong LiuDepartment of Radiotherapy, Linyi Cancer Hospital, Lingyuan East Street No. 6, Lanshan District, Linyi 276400, Shandong Province, People’s Republic of ChinaEmail yiyan0663363@163.comBackground: Long non-coding RNA (lncRNA) exerts a regulatory role in the occurrence and progression of tumors. This study aimed at probing into the function and mechanism of lncRNA DICER1 antisense RNA 1 (DICER1-AS1) in colorectal cancer (CRC).Methods: The expressions of DICER1-AS1, miR-296-5p and STAT3 mRNA were tested by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay was employed to detect cell proliferation, and Transwell was used to detect cell migration and invasion. In addition, the expressions of apoptosis-related proteins Bax and Bcl2 were detected by Western blot. Interactions between DICER1-AS1 and miR-296-5p, and miR-296-5p and STAT3 were predicted and determined by bioinformatics analysis, luciferase reporter assay and RNA binding protein immunoprecipitation (RIP) assay.Results: The expressions of DICER1-AS1 and STAT3 mRNA were significantly up-regulated while miR-296-5p expression was remarkably down-regulated in CRC tissues and cell lines. Over-expression of DICER1-AS1 or transfection of miR-296-5p inhibitors could promote the proliferation, migration and invasion and inhibit apoptosis of CRC cells, whereas knockdown of DICER1-AS1 or transfection of miR-296-5p mimics had the opposite effects. Additionally, DICER1-AS1 could down-regulate miR-296-5p expression via sponging it. DICER1-AS1 also enhanced the expression of STAT3, which was identified as a target gene of miR-296-5p.Conclusion: DICER1-AS1 acts as an oncogenic lncRNA in CRC via modulating miR-296-5p/STAT3 axis. Our results provide a new direction for the diagnosis and treatment of CRC.Keywords: DICER1-AS1, miR-296-5p, CRC, proliferation, metastasis

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