Diagnostic reliability of different RT-PCR protocols for the detection of bluetongue virus serotype 14 (BTV-14)

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Opis

Tytuł:: Diagnostic reliability of different RT-PCR protocols for the detection of bluetongue virus serotype 14 (BTV-14)
Autorzy:: Orłowska Anna
Żmudziński Jan F.
Smreczak Marcin
Trębas Paweł
Marzec Anna
Temat:: bluetongue
typing
genetic variability
poland
Veterinary medicine
SF600-1100
Źródło:: Journal of Veterinary Research, Vol 61, Iss 4, Pp 391-395 (2017)
Wydawca:: Sciendo, 2017.
Rok publikacji:: 2017
Kolekcja:: LCC:Veterinary medicine
Typ dokumentu:: article
Opis pliku:: electronic resource
Język:: English
ISSN:: 2450-8608
Relacje:: https://doaj.org/toc/2450-8608
DOI:: 10.1515/jvetres-2017-0065
Dostęp URL:: https://doaj.org/article/966b174382ac41bfa9ab1d17bcc0eeb9 Link otwiera się w nowym oknie
Numer akcesji:: edsdoj.966b174382ac41bfa9ab1d17bcc0eeb9
: Czasopismo naukowe

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Introduction: The reverse transcription polymerase chain reaction (RT-PCR) is one of the most extensively used methods for identification of animals infected with bluetongue virus (BTV). There are several RT-PCR protocols published and several real-time RT-PCR (rtRT-PCR) commercial kits available on the market. Because Poland faced BTV-14 infection in 2012, different protocols were implemented in the country to confirm the RT-PCR results positive for this virus. The article presents a comparative study of several RT-PCR protocols and discusses their diagnostic reliability and applicability.

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