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Tytuł pozycji:

Alteration of the Immune Microenvironment in HBsAg and HBeAg Dual-Positive Pregnant Women Presenting a High HBV Viral Load

Tytuł:
Alteration of the Immune Microenvironment in HBsAg and HBeAg Dual-Positive Pregnant Women Presenting a High HBV Viral Load
Autorzy:
Gao F
Wang H
Li X
Guo F
Yuan Y
Wang X
Zhang Y
Bai G
Temat:
single-cell rna sequencing
hbv infection
pregnancy
immune cells
cd8+ t cells
Pathology
RB1-214
Therapeutics. Pharmacology
RM1-950
Źródło:
Journal of Inflammation Research, Vol Volume 14, Pp 5619-5632 (2021)
Wydawca:
Dove Medical Press, 2021.
Rok publikacji:
2021
Kolekcja:
LCC:Pathology
LCC:Therapeutics. Pharmacology
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
1178-7031
Relacje:
https://www.dovepress.com/alteration-of-the-immune-microenvironment-in-hbsag-and-hbeag-dual-posi-peer-reviewed-fulltext-article-JIR; https://doaj.org/toc/1178-7031
Dostęp URL:
https://doaj.org/article/97ab8eaa124e4c3fb4c8a60698cecb93  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.97ab8eaa124e4c3fb4c8a60698cecb93
Czasopismo naukowe
Fan Gao,1,* Hongyan Wang,2,* Xia Li,2 Fanfan Guo,2 Yufei Yuan,2 Xiaona Wang,2 Yidan Zhang,2 Guiqin Bai2 1Clinical Research Center, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China; 2Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guiqin BaiDepartment of Gynecology and Obstetrics, The First Affiliated Hospital of Xi’an Jiaotong University, 277 Yanta West Road, Xi’an, 710061, Shaanxi, People’s Republic of ChinaTel +86-18991232517Email baigq@126.comPurpose: The aim of this study was to compare the differences in the immune microenvironment between HBV-infected pregnant women with a high HBV viral load and healthy pregnant women, with an emphasis on T cell subset alteration.Patients and Methods: We compared the differences of cellular and molecular signatures between HBV-infected and healthy pregnant women by performing single-cell RNA and T cell receptor sequencing of peripheral blood mononuclear cells from 51,836 women in the mid-trimester pregnancy stage. Specific trajectories of the different T clusters throughout the course of T cell differentiation were investigated. Flow cytometry was used to validate the proportion of different T cell subtypes.Results: We identified nine cellular subtypes and found an increased proportion of effector/memory CD8+ T cells in HBV-infected pregnant women. Both CD4+ and CD8+ effector/memory T cells in HBV-related samples expressed higher levels of metallothionein (MT)-related genes (MT2A, MTIE, MTIF, MTIX), metal ion pathways, and multiple inflammatory responses. Among CD8+ T cell clusters, we identified a particular subset of effector/memory CD8+ T cells (CD8-cluster 2) with MTs as the top-ranking genes, which may be enriched in HBV-related samples. These cells showed an increased clonal expansion in HBV infection. Moreover, we found more active immune responses, according to cellular interaction patterns, between immune cell subsets in HBV-infected samples.Conclusion: This study shows significant differences between HBV-infected and healthy samples, including cell clusters, dominant gene sets, T cell function, clonal expansion, and V/J gene usage of T cell clonotypes, and identifies a distinct CD8+ T cell cluster with immune-active and antiviral properties. These findings pave the way for a deeper understanding of the impact of HBV infection on the immune microenvironment during pregnancy.Keywords: single-cell RNA sequencing, HBV infection, pregnancy, immune cells, CD8+ T cells

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