Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs

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Opis

Tytuł:: Protocol to Reprogram Human Menstrual Blood-Derived Stromal Cells to Generate AOX15-iPSCs
Autorzy:: Alicia Sanzhez-Mata
Alberto Ferez-Gomez
Elena Gonzalez-Muñoz
Temat:: Cell culture
Stem Cells
Cell Differentiation
Science (General)
Q1-390
Źródło:: STAR Protocols, Vol 1, Iss 3, Pp 100183- (2020)
Wydawca:: Elsevier, 2020.
Rok publikacji:: 2020
Kolekcja:: LCC:Science (General)
Typ dokumentu:: article
Opis pliku:: electronic resource
Język:: English
ISSN:: 2666-1667
Relacje:: http://www.sciencedirect.com/science/article/pii/S2666166720301702; https://doaj.org/toc/2666-1667
DOI:: 10.1016/j.xpro.2020.100183
Dostęp URL:: https://doaj.org/article/97d7c75d193e4f7488f51fc857029e0a Link otwiera się w nowym oknie
Numer akcesji:: edsdoj.97d7c75d193e4f7488f51fc857029e0a
: Czasopismo naukowe

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Summary: Cell reprogramming has revolutionized the fields of cell and regenerative biology. However, human induced pluripotent stem cell (iPSC) derivation remains inefficient and variable. Here, we present a protocol that uses human menstrual blood-derived stromal cells (MnSCs), which are susceptible to reprogramming, as a source of somatic cells. We describe an oocyte-based reprogramming combination to generate AOX15-iPSCs that can be used to study different states of pluripotency.For complete details on the use and execution of this protocol, please refer to Lopez-Caraballo et al. (2020).

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