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Tytuł pozycji:

Cold seawater induces early sexual developmental stages in the BPG axis of European eel males

Tytuł:
Cold seawater induces early sexual developmental stages in the BPG axis of European eel males
Autorzy:
Christoffer Rozenfeld
Víctor García-Carpintero
Luz Pérez
Victor Gallego
Juan Germán Herranz-Jusdado
Helge Tveiten
Helge K. Johnsen
Romain Fontaine
Finn-Arne Weltzien
Joaquín Cañizares
Juan F. Asturiano
David S. Peñaranda
Temat:
Anguilla anguilla
RNA-sequencing
Epigenetics
Temperature
Spermatogonial proliferation
Migration
Biotechnology
TP248.13-248.65
Genetics
QH426-470
Źródło:
BMC Genomics, Vol 20, Iss 1, Pp 1-20 (2019)
Wydawca:
BMC, 2019.
Rok publikacji:
2019
Kolekcja:
LCC:Biotechnology
LCC:Genetics
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
1471-2164
Relacje:
http://link.springer.com/article/10.1186/s12864-019-5969-6; https://doaj.org/toc/1471-2164
DOI:
10.1186/s12864-019-5969-6
Dostęp URL:
https://doaj.org/article/9948641eb6a64ee491e144c95c7cd9a9  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.9948641eb6a64ee491e144c95c7cd9a9
Czasopismo naukowe
Abstract Background The impossibility of closing the life cycle of the European eel (Anguilla anguilla) in captivity troubles the future of this critically endangered species. In addition, the European eel is a highly valued and demanded resource, thus the successful closing of its life cycle would have a substantial economic and ecological impact. With the aim of obtaining the highest gamete quality, the study of the effects of environmental factors, such as temperature, on reproductive performance may prove valuable. This is especially true for the exposure to cold water, which has been reported to improve sexual development in multiple other Actinopterygii species. Results European eel males treated with cold seawater (10 °C, T10) for 2 weeks showed an increase in the proliferation and differentiation of spermatogonial cells until the differentiated spermatogonial type A cell stage, and elevated testosterone and 11-ketotestosterone plasma levels. Transcriptomes from the tissues of the brain-pituitary-gonad (BPG) axis of T10 samples revealed a differential gene expression profile compared to the other experimental groups, with clustering in a principal component analysis and in heat maps of all differentially expressed genes. Furthermore, a functional analysis of differentially expressed genes revealed enriched gene ontology terms involved in the regulation of circadian rhythm, histone modification, meiotic nuclear division, and others. Conclusions Cold seawater treatment had a clear effect on the activity of the BPG-axis of European eel males. In particular, our cold seawater treatment induces the synchronization and increased proliferation and differentiation of specific spermatogonial cells. In the transcriptomic results, genes related to thermoception were observed. This thermoception may have caused the observed effects through epigenetic mechanisms, since all analysed tissues further revealed differentially expressed genes involved in histone modification. The presented results support our hypothesis that a low temperature seawater treatment induces an early sexual developmental stage in European eels. This hypothesis is logical given that the average temperature experienced by eels in the early stages of their oceanic reproductive migration is highly similar to that of this cold seawater treatment. Further studies are needed to test whether a cold seawater treatment can improve the response of European eels to artificial hormonal treatment, as the results suggest.
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