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Tytuł pozycji:

Spent media analysis suggests cultivated meat media will require species and cell type optimization

Tytuł:
Spent media analysis suggests cultivated meat media will require species and cell type optimization
Autorzy:
Edward N. O’Neill
Joshua C. Ansel
Grace A. Kwong
Michael E. Plastino
Jenny Nelson
Keith Baar
David E. Block
Temat:
Nutrition. Foods and food supply
TX341-641
Food processing and manufacture
TP368-456
Źródło:
npj Science of Food, Vol 6, Iss 1, Pp 1-10 (2022)
Wydawca:
Nature Portfolio, 2022.
Rok publikacji:
2022
Kolekcja:
LCC:Nutrition. Foods and food supply
LCC:Food processing and manufacture
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
2396-8370
Relacje:
https://doaj.org/toc/2396-8370
DOI:
10.1038/s41538-022-00157-z
Dostęp URL:
https://doaj.org/article/9bcf9e1439b74db69d4d7dc6eb450b1f  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.9bcf9e1439b74db69d4d7dc6eb450b1f
Czasopismo naukowe
Abstract Cell culture media design is perhaps the most significant hurdle currently facing the commercialization of cultivated meat as an alternative source of dietary protein. Since media optimization for a specific culture system requires a significant amount of effort and investment, a major question remaining is whether media formulations can be easily shared across multiple production schemes for cells of different species and lineages. Here, we perform spent medium analysis to compare the specific nutrient utilization of primary embryonic chicken muscle precursor cells and fibroblasts to the murine C2C12 myoblast cell line. We demonstrate that these related cell types have significantly different nutrient utilization patterns collectively and on a per-cell basis, and that many components of conventional media do not appear to be depleted by the cells. Namely, glucose was not consumed as rapidly nor as completely by the chicken muscle precursors compared to other cells overall, and there were significant differences in specific consumption rates for several other key nutrients over the first day of culture. Ultimately, our results indicate that no one medium is likely ideal and cost effective to culture multiple cell types and that novel methods to streamline media optimization efforts will be important for the industry to develop.

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