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Title of the item:

Tetramethylpyrazine and Paeoniflorin Inhibit Oxidized LDL-Induced Angiogenesis in Human Umbilical Vein Endothelial Cells via VEGF and Notch Pathways

Title :
Tetramethylpyrazine and Paeoniflorin Inhibit Oxidized LDL-Induced Angiogenesis in Human Umbilical Vein Endothelial Cells via VEGF and Notch Pathways
Authors :
Rong Yuan
Weili Shi
Qiqi Xin
Binrui Yang
Maggie Puiman Hoi
Simon Mingyuan Lee
Weihong Cong
Keji Chen
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Subject Terms :
Other systems of medicine
RZ201-999
Source :
Evidence-Based Complementary and Alternative Medicine, Vol 2018 (2018)
Publisher :
Hindawi Limited, 2018.
Publication Year :
2018
Collection :
LCC:Other systems of medicine
Document Type :
article
File Description :
electronic resource
Language :
English
ISSN :
1741-427X
1741-4288
Relation :
https://doaj.org/toc/1741-427X; https://doaj.org/toc/1741-4288
DOI :
10.1155/2018/3082507
Access URL :
https://doaj.org/article/a9e443753c544f5696174509f8a2d006
Accession Number :
edsdoj.9e443753c544f5696174509f8a2d006
Academic Journal
Atherosclerotic plaque angiogenesis is key factor in plaque instability and vulnerability, and low concentrations of oxidized low density lipoprotein (ox-LDL) promote the in vitro angiogenesis of endothelial cells and play an important role in plaque angiogenesis. Ligusticum chuanxiong Hort. and Radix Paeoniae Rubra herb pair in Chinese medicine obtains the optimum therapeutic efficacy in atherosclerosis, and their major active ingredients tetramethylpyrazine (TMP) and paeoniflorin (PF) are reported to alleviate atherosclerosis. The aim of this study was to investigate the effects of TMP and PF on ox-LDL-induced angiogenesis and the underlying mechanism. Human umbilical vein endothelial cells (HUVECs) were incubated with ox-LDL and were then treated with TMP, PF, or a combination of TMP and PF. Cell proliferation, migration, tube formation, and the expression of angiogenesis-related proteins were measured. Synergism was evaluated using the combination index in cell proliferation. We found that TMP and PF attenuated the in vitro angiogenesis in ox-LDL-induced HUVECs. In addition, the combination of TMP and PF not only inhibited the ox-LDL-induced expression of CD31, vascular endothelial growth factor (VEGF), and VEGF receptor 2 (VEGFR2) but also decreased the ox-LDL-induced expression of Notch1, Jagged1, and Hes1. In summary, the combination of TMP and PF suppresses ox-LDL-induced angiogenesis in HUVECs by inhibiting both the VEGF/VEGFR2 and the Jagged1/Notch1 signaling pathways, which might contribute to the stability of plaques in atherosclerosis.

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