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Tytuł pozycji:

Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer

Tytuł :
Enhanced expression of G-protein coupled estrogen receptor (GPER/GPR30) in lung cancer
Autorzy :
Jala Venkatakrishna Rao
Radde Brandie N
Haribabu Bodduluri
Klinge Carolyn M
Pokaż więcej
Temat :
GPER
GPR30
Estrogen
Estrogen receptor
Lung cancer
Protein expression
Immunohistochemistry
Tissue microarray
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RC254-282
Źródło :
BMC Cancer, Vol 12, Iss 1, p 624 (2012)
Wydawca :
BMC, 2012.
Rok publikacji :
2012
Kolekcja :
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Typ dokumentu :
article
Opis pliku :
electronic resource
Język :
English
ISSN :
1471-2407
Relacje :
http://www.biomedcentral.com/1471-2407/12/624; https://doaj.org/toc/1471-2407
DOI :
10.1186/1471-2407-12-624
Dostęp URL :
https://doaj.org/article/b1e65ddee5404b8eb470e658ed61c66b
Prawa :
Journal Licence: CC BY
Numer akcesji :
edsdoj.b1e65ddee5404b8eb470e658ed61c66b
Czasopismo naukowe
Abstract Background G-protein-coupled estrogen receptor (GPER/GPR30) was reported to bind 17β-estradiol (E2), tamoxifen, and ICI 182,780 (fulvestrant) and promotes activation of epidermal growth factor receptor (EGFR)-mediated signaling in breast, endometrial and thyroid cancer cells. Although lung adenocarcinomas express estrogen receptors α and β (ERα and ERβ), the expression of GPER in lung cancer has not been investigated. The purpose of this study was to examine the expression of GPER in lung cancer. Methods The expression patterns of GPER in various lung cancer lines and lung tumors were investigated using standard quantitative real time PCR (at mRNA levels), Western blot and immunohistochemistry (IHC) methods (at protein levels). The expression of GPER was scored and the pairwise comparisons (cancer vs adjacent tissues as well as cancer vs normal lung tissues) were performed. Results Analysis by real-time PCR and Western blotting revealed a significantly higher expression of GPER at both mRNA and protein levels in human non small cell lung cancer cell (NSCLC) lines relative to immortalized normal lung bronchial epithelial cells (HBECs). The virally immortalized human small airway epithelial cell line HPL1D showed higher expression than HBECs and similar expression to NSCLC cells. Immunohistochemical analysis of tissue sections of murine lung adenomas as well as human lung adenocarcinomas, squamous cell carcinomas and non-small cell lung carcinomas showed consistently higher expression of GPER in the tumor relative to the surrounding non-tumor tissue. Conclusion The results from this study demonstrate increased GPER expression in lung cancer cells and tumors compared to normal lung. Further evaluation of the function and regulation of GPER will be necessary to determine if GPER is a marker of lung cancer progression.

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