Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Tytuł pozycji:

Effect of process parameters on additive-free electrospinning of regenerated silk fibroin nonwovens

Tytuł:
Effect of process parameters on additive-free electrospinning of regenerated silk fibroin nonwovens
Autorzy:
Alexander Kopp
Ralf Smeets
Martin Gosau
Nadja Kröger
Sandra Fuest
Marius Köpf
Magnus Kruse
Judith Krieger
Rico Rutkowski
Anders Henningsen
Simon Burg
Temat:
Fibroin
Additive-free electrospinning
Nanofiber
Silk
Scaffolds
Materials of engineering and construction. Mechanics of materials
TA401-492
Biology (General)
QH301-705.5
Źródło:
Bioactive Materials, Vol 5, Iss 2, Pp 241-252 (2020)
Wydawca:
KeAi Communications Co., Ltd., 2020.
Rok publikacji:
2020
Kolekcja:
LCC:Materials of engineering and construction. Mechanics of materials
LCC:Biology (General)
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
2452-199X
Relacje:
http://www.sciencedirect.com/science/article/pii/S2452199X20300104; https://doaj.org/toc/2452-199X
DOI:
10.1016/j.bioactmat.2020.01.010
Dostęp URL:
https://doaj.org/article/eb395a7effcd471b8b1363e71e87907a  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.b395a7effcd471b8b1363e71e87907a
Czasopismo naukowe
Silk fibroin is a biomaterial with multiple beneficial properties for use in regenerative medicine and tissue engineering. When dissolving and processing the reconstituted silk fibroin solution by electrospinning, the arrangement and size of fibers can be manifold varied and according fiber diameters reduced to the nanometer range. Such nonwovens show high porosity as well as potential biocompatibility. Usually, electrospinning of most biomaterials demands for the application of additives, which enable stable electrospinning by adjusting viscosity, and are intended to evaporate during processing or to be washed out afterwards. However, the use of such additives increases costs and has to be taken into account in terms of biological risks when used for biomedical applications.In this study, we explored the possibilities of additive-free electrospinning of pure fibroin nonwovens and tried to optimize process parameters to enable stable processing. We used natural silk derived from the mulberry silkworm Bombyx mori. After degumming, the silk fibroin was dissolved and the viscosity of the spinning solution was controlled by partial evaporation of the initial solving agent. This way, we were able to completely avoid the use of additives and manufacture nonwovens, which potentially offer higher biocompatibility and reduced immunogenicity. Temperature and relative humidity during electrospinning were systematically varied (25–35 °C, 25–30% RH). In a second step, the nonwovens optionally underwent methanol treatment to initiate beta-sheet formation in order to increase structural integrity and strength. Comprehensive surface analysis on the different nonwovens was performed using scanning electron microscopy and supplemented by additional mechanical testing. Cytotoxicity was evaluated using BrdU-assay, XTT-assay, LDH-assay and live-dead staining. Our findings were, that an increase of temperature and relative humidity led to unequal fiber diameters and defective nonwovens. Resistance to penetration decreased accordingly. The most uniform fiber diameters of 998 ± 63 nm were obtained at 30 °C and 25% relative humidity, also showing the highest value for resistance to penetration (0.20 N). The according pure fibroin nonwoven also showed no signs of cytotoxicity. However, while the biological response showed statistical evidence, the material characteristics showed no statistically significant correlation to changes of the ambient conditions within the investigated ranges. We suggest that further experiments should explore additional ranges for temperature and humidity and further focus on the repeatability of material properties in dependency of suitable process windows.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies