Information

Dear user, the application need JavaScript support. Please enable JavaScript in your browser.

You are browsing as a GUEST
Title of the item:

Label-Free Mass Spectrometry-Based Quantification of Linker Histone H1 Variants in Clinical Samples

Title :
Label-Free Mass Spectrometry-Based Quantification of Linker Histone H1 Variants in Clinical Samples
Authors :
Roberta Noberini
Cristina Morales Torres
Evelyn Oliva Savoia
Stefania Brandini
Maria Giovanna Jodice
Giovanni Bertalot
Giuseppina Bonizzi
Maria Capra
Giuseppe Diaferia
Paola Scaffidi
Tiziana Bonaldi
Show more
Subject Terms :
epigenetics
label-free quantification
linker histone H1
mass spectrometry
proteomics
laser micro-dissection
Biology (General)
QH301-705.5
Chemistry
QD1-999
Source :
International Journal of Molecular Sciences, Vol 21, Iss 7330, p 7330 (2020)
Publisher :
MDPI AG, 2020.
Publication Year :
2020
Collection :
LCC:Biology (General)
LCC:Chemistry
Document Type :
article
File Description :
electronic resource
Language :
English
ISSN :
1422-0067
1661-6596
Relation :
https://www.mdpi.com/1422-0067/21/19/7330; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067
DOI :
10.3390/ijms21197330
Access URL :
https://doaj.org/article/b9a0c5cbda5b4b93b11ada2c53d1a6c2
Accession Number :
edsdoj.b9a0c5cbda5b4b93b11ada2c53d1a6c2
Academic Journal
Epigenetic aberrations have been recognized as important contributors to cancer onset and development, and increasing evidence suggests that linker histone H1 variants may serve as biomarkers useful for patient stratification, as well as play an important role as drivers in cancer. Although traditionally histone H1 levels have been studied using antibody-based methods and RNA expression, these approaches suffer from limitations. Mass spectrometry (MS)-based proteomics represents the ideal tool to accurately quantify relative changes in protein abundance within complex samples. In this study, we used a label-free quantification approach to simultaneously analyze all somatic histone H1 variants in clinical samples and verified its applicability to laser micro-dissected tissue areas containing as low as 1000 cells. We then applied it to breast cancer patient samples, identifying differences in linker histone variants patters in primary triple-negative breast tumors with and without relapse after chemotherapy. This study highlights how label-free quantitation by MS is a valuable option to accurately quantitate histone H1 levels in different types of clinical samples, including very low-abundance patient tissues.
Please log in to access the full text.

We use cookies to help identify your computer so we can tailor your user experience, track shopping basket contents and remember where you are in the order process.