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Tytuł pozycji:

miR-525-5p Modulates Proliferation and Epithelial–Mesenchymal Transition of Glioma by Targeting Stat-1

Tytuł:
miR-525-5p Modulates Proliferation and Epithelial–Mesenchymal Transition of Glioma by Targeting Stat-1
Autorzy:
Xie P
Han Q
Liu D
Yao D
Lu X
Wang Z
Zuo X
Temat:
mir-525-5p
glioma
stat 1
proliferation
emt
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
RC254-282
Źródło:
OncoTargets and Therapy, Vol Volume 13, Pp 9957-9966 (2020)
Wydawca:
Dove Medical Press, 2020.
Rok publikacji:
2020
Kolekcja:
LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Typ dokumentu:
article
Opis pliku:
electronic resource
Język:
English
ISSN:
1178-6930
Relacje:
https://www.dovepress.com/mir-525-5p-modulates-proliferation-and-epithelialndashmesenchymal-tran-peer-reviewed-article-OTT; https://doaj.org/toc/1178-6930
Dostęp URL:
https://doaj.org/article/ff9e826b92ae476d9dbeea3128fd645f  Link otwiera się w nowym oknie
Numer akcesji:
edsdoj.ff9e826b92ae476d9dbeea3128fd645f
Czasopismo naukowe
Peng Xie,1,* Qiu Han,2,* Dachao Liu,3,* Dan Yao,4 Xiaoqing Lu,5 Ziyu Wang,6 Xiaohua Zuo7 1Department of Neurosurgery, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 2Department of Neurology, Huai’an First People’s Hospital, The Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu 223000, People’s Republic of China; 3Department of Image, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 4Department of General Surgery, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 5Department of Orthopedic, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 6Department of Emergency Intensive Care Unit, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China; 7Department of Pain Management, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, Huai’an 223002, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xiaohua ZuoDepartment of Pain Management, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, No. 62, Huaihai Road (S.), Huai’an 223002, People’s Republic of ChinaEmail xiaohuazuo@yahoo.comZiyu WangDepartment of Emergency Intensive Care Unit, The Affiliated Huai’an Hospital of Xuzhou Medical University, The Second People’s Hospital of Huai’an, No. 62, Huaihai Road (S.), Huai’an 223002, People’s Republic of ChinaEmail 495366275@qq.comBackground: Glioma is the most aggressive human brain tumor. Recent studies revealed that microRNAs play vital roles in glioma. However, the function of microRNA-525-5p (miR-525-5p) in glioma remains unclear.Methods: qRT-PCR and Western blotting were used to evaluate mRNA and protein levels in glioma tissues and cells. Colony formation, CCK-8, and Edu assays evaluated the growth of glioma cells. Wound-healing, transwell, and 3D invasion assays examined the migration and invasion activities of glioma cells. Luciferase reporter assays assessed the regulatory relationship interaction between miR-525-5p and Stat-1. A mouse xenograft model was used to examine the effect of miR-525-5p on glioma in vivo.Results: miR-525-5p expression was downregulated in glioma tissues and cells. Overexpressing miR-525-5p decreased the growth of glioma cells and reduced the migration, invasion, and epithelial–mesenchymal transition of glioma cells. Bioinformatics analysis identified Stat-1 as a potential target of miR-525-5p, and dual luciferase reporter assays revealed that miR-525-5p negatively regulates Stat-1. Decreased Stat-1 led to the inhibition of FOXM1, affecting NF-κB signaling activity. Overexpressing miR-525-5p reduced tumor development in vivo.Conclusion: miR-525-5p negatively regulates cell proliferation, migration, invasion, and epithelial–mesenchymal transition in glioma, and Stat 1 is a target of miR-525-5p. miR-525-5p may be a potential target for glioma treatment.Keywords: miR-525-5p, glioma, Stat 1, proliferation, EMT
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