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Tytuł pozycji:

Interleukin-6 affects insulin secretion and glucose metabolism of rat pancreatic islets in vitro.

Tytuł :
Interleukin-6 affects insulin secretion and glucose metabolism of rat pancreatic islets in vitro.
Index Terms :
Sciences bio-médicales et agricoles
Adenine Nucleotides -- metabolism
Adenosine Triphosphate -- metabolism
Animals
Cells, Cultured
DNA -- metabolism
Energy Metabolism
Glucose -- metabolism
Insulin -- secretion
Interleukin-1 -- pharmacology
Interleukin-6 -- pharmacology
Islets of Langerhans -- metabolism
Male
Oxidation-Reduction
Oxygen Consumption
Proinsulin -- biosynthesis
Protein Biosynthesis
Rats
Rats, Inbred Strains
Recombinant Proteins
info:eu-repo/semantics/article
info:ulb-repo/semantics/articlePeerReview
info:ulb-repo/semantics/openurl/article
Wydawca :
1990
Dodane szczegóły :
Sandler, S
Bendtzen, K
Eizirik, Decio L.
Welsh, M
Typ dokumentu :
Zasób elektroniczny
URL :
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/55972
http://worldcat.org/search?q=on:EQY+http://difusion-oai.ulb.ac.be/oai/request+DCG_ENTIRE_REPOSITORY+CNTCOLL
Dostępność :
Open access content. Open access content
Pozostałe numery :
EQY oai:dipot.ulb.ac.be:2013/55972
uri/info:pmid/2404746
764603025
Źródło wspomagające :
UNIV LIBR DE BRUXELLES
From OAIster®, provided by the OCLC Cooperative.
Numer akcesji :
edsoai.ocn764603025
Zasób elektroniczny
Recently it has been postulated that interleukin-1 (IL-1) locally released by infiltrating mononuclear cells may destroy the pancreatic B cells during the development of insulin-dependent diabetes mellitus. Since IL-1 is a potent inducer of interleukin-6 (IL-6) in various cells, it is conceivable that IL-6 is a second mediator of the IL-1 action. In the present study the effects of IL-6 alone or in combination with IL-1 were studied on pancreatic islet function in vitro after tissue culture and compared with the effects observed after exposure to IL-1 only. Rat pancreatic islets were cultured in medium RPMI 1640 + 10% calf serum with or without the addition of human recombinant IL-6 (500-5000 pg/ml) for 48 h. The medium insulin accumulation was increased by 40-50% after culture with 500-2000 pg/ml IL-6, but was similar to the controls at 5000 pg/ml. When islets were cultured for 18 h only, also 5000 pg/ml IL-6 stimulated the medium insulin accumulation. IL-6 did not affect the islet insulin content and the rates of islet (pro)insulin and total protein biosynthesis. It inconsistently decreased the islet DNA content. In short-term experiments after 48-h culture with IL-6, there was a dose-dependent inhibition of the glucose-stimulated insulin release. On the other hand, islets cultured with IL-6 (5000 pg/ml) exhibited an elevated glucose oxidation and oxygen uptake, but a lower ATP content at 16.7 mM glucose and an unaffected glucose utilization and glutamine oxidation compared to the controls. This raises the possibility that IL-6 had induced a condition with an increased energy expenditure, resulting in an enhanced mitochondrial metabolism of glucose. Islets cultured with human recombinant IL-1 beta (25 units/ml) showed a strong inhibition of the insulin accumulation in the culture medium and of glucose-stimulated insulin release and a marked decrease in the islet DNA and insulin content. A combination of IL-1 (25 U/ml) + IL-6 (1000 pg/ml) did not alter the inhibito
Journal Article
Research Support, Non-U.S. Gov't
info:eu-repo/semantics/published

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