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Title of the item:

Flap loop of GluD2 binds to Cbln1 and induces presynaptic differentiation

Title :
Flap loop of GluD2 binds to Cbln1 and induces presynaptic differentiation
Index Terms :
Glutamate receptor δ2
N-terminal domain
Flap loop
Cbln1
Synapse formation
Amino Acid Sequence
Animals
Cell Line
Humans
Mice
Molecular Sequence Data
Nerve Tissue Proteins/chemistry
Nerve Tissue Proteins/metabolism
Neurogenesis
Protein Precursors/chemistry
Protein Precursors/metabolism
Protein Structure, Secondary
Receptors, Glutamate/chemistry
Receptors, Glutamate/genetics
Receptors, Glutamate/metabolism
Synapses/physiology
Journal Article
Source :
Biochemical and biophysical research communications
Publisher :
Elsevier Inc. 2010-10-19T06:38:57Z 2010-10-19T06:38:57Z 2010-07-30
Added Details :
平野, 丈夫
Kuroyanagi, Tomoaki
Hirano, Tomoo
Document Type :
Electronic Resource
URL :
http://hdl.handle.net/2433/128851
10.1016/j.bbrc.2010.06.114
Availability :
© 2010 Elsevier Inc.
Other Numbers :
YJ@ oai:repository.kulib.kyoto-u.ac.jp:2433/128851
0006-291X
AA00564395
Biochemical and biophysical research communications
398
3
537
541
20599760
826573530
Contributing Source :
From OAIster®, provided by the OCLC Cooperative.
Accession Number :
edsoai.ocn826573530
Electronic Resource
Glutamate receptor delta2 (GluD2) is selectively expressed on the postsynaptic spines at parallel-fiber (PF)-Purkinje neuron (PN) synapses. GluD2 knockout mice show a reduced number of PF-PN synapses, suggesting that GluD2 is involved in synapse formation. Recent studies revealed that GluD2 induces presynaptic differentiation in a manner dependent on its N-terminal domain (NTD) through binding of Cbln1 secreted from cerebellar granule neurons. However, the underlying mechanism of the specific binding of the NTD to Cbln1 remains elusive. Here, we have identified the flap loop (Arg321-Trp339) in the NTD of GluD2 (GluD2-NTD) as a crucial region for the binding to Cbln1 and the induction of presynaptic differentiation. Both induction of presynaptic differentiation and binding of Cbln1 were abolished in the HEK cells expressing not wild-type GluD2 but GluD2 with mutations in the flap loop. Especially, single amino acid substitution of either Arg321 or Trp323 to alanine was sufficient to disable the GluD2 function. Finally, a homology model of GluD2-NTD suggested that the flap loop is located at the distal end, which appears consistent with an interaction with Cbln1 and a presynaptic varicosity.

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