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Tytuł pozycji:

COUP-TF interacting protein 2 represses the initial phase of HIV-1 gene transcription in human microglial cells.

Tytuł :
COUP-TF interacting protein 2 represses the initial phase of HIV-1 gene transcription in human microglial cells.
Index Terms :
Sciences bio-médicales et agricoles
COUP Transcription Factors
Carrier Proteins -- physiology
Cell Line
Cell Nucleus Structures -- chemistry
Chromosomal Proteins, Non-Histone -- analysis
DNA-Binding Proteins -- analysis
DNA-Binding Proteins -- antagonists & inhibitors
DNA-Binding Proteins -- chemistry
DNA-Binding Proteins -- physiology
Gene Expression Regulation, Viral
HIV Long Terminal Repeat
HIV-1 -- genetics
HIV-1 -- physiology
Humans
Microglia -- metabolism
Microglia -- virology
Nuclear Proteins -- physiology
Protein Structure, Tertiary
Receptors, Steroid -- analysis
Receptors, Steroid -- antagonists & inhibitors
Receptors, Steroid -- chemistry
Repressor Proteins -- analysis
Repressor Proteins -- physiology
Sp1 Transcription Factor -- analysis
Sp1 Transcription Factor -- antagonists & inhibitors
Sp1 Transcription Factor -- chemistry
Transcription Factors -- analysis
Transcription Factors -- antagonists & inhibitors
Transcription Factors -- chemistry
Transcription, Genetic
Tumor Suppressor Proteins
Virus Replication
info:eu-repo/semantics/article
info:ulb-repo/semantics/articlePeerReview
info:ulb-repo/semantics/openurl/article
Wydawca :
2005
Dodane szczegóły :
Marban, Céline
Redel, Laetitia
Suzanne, Stella
Van Lint, Carine
Lecestre, Dominique
Chasserot-Golaz, Sylvette
Leid, Mark
Aunis, Dominique
Schaeffer, Evelyne
Rohr, Olivier
Typ dokumentu :
Zasób elektroniczny
URL :
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/51741">http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/51741
http://worldcat.org/search?q=on:EQY+http://difusion-oai.ulb.ac.be/oai/request+DCG_ENTIRE_REPOSITORY+CNTCOLL">http://worldcat.org/search?q=on:EQY+http://difusion-oai.ulb.ac.be/oai/request+DCG_ENTIRE_REPOSITORY+CNTCOLL
Dostępność :
Open access content. Open access content
1 full-text file(s): info:eu-repo/semantics/restrictedAccess
Pozostałe numery :
EQY oai:dipot.ulb.ac.be:2013/51741
855618151
Źródło wspomagające :
UNIV LIBR DE BRUXELLES
From OAIster®, provided by the OCLC Cooperative.
Numer akcesji :
edsoai.ocn855618151
Zasób elektroniczny
Human immunodeficiency virus type 1 (HIV-1) gene transcription is characterized by two temporally distinct phases. While the initial phase relies solely on cellular transcription factors, the subsequent phase is activated by the viral Tat transactivator. We have previously reported that the subsequent phase of viral gene transcription can be repressed by the chicken ovalbumin upstream promoter transcription factor (COUP-TF)-interacting protein 2 (CTIP2) in human microglial cells [O. Rohr, D. Lecestre, S. Chasserot-Golaz, C. Marban, D. Avram, D. Aunis, M. Leid and E. Schaeffer (2003), J. Virol. 77, 5415-5427]. Here, we demonstrate that CTIP proteins also repress the initial phase of HIV-1 gene transcription, mainly supported by the cellular transcription factors Sp1 and COUP-TF in microglial cells. We report that CTIP2 represses Sp1- and COUP-TF-mediated activation of HIV-1 gene transcription and viral replication as a result of physical interactions with COUP-TF and Sp1 in microglial nuclei. Using laser confocal microscopy CTIP2 was found to colocalize with Sp1, COUP-TF and the heterochromatin-associated protein Hp1alpha, which is mainly detected in transcriptionally repressed heterochromatic region. Moreover, we describe that CTIP2 can be recruited to the HIV-1 promoter via its association with Sp1 bound to the GC-box sequences of the long terminal repeat (LTR). Since our findings demonstrate that CTIP2 interacts with the HIV-1 proximal promoter, it is likely that CTIP2 promotes HIV-1 gene silencing by forcing transcriptionally repressed heterochromatic environment to the viral LTR region.
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.
SCOPUS: ar.j
info:eu-repo/semantics/published

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