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Tytuł pozycji:

Transcription factor binding sites in the pol gene intragenic regulatory region of HIV-1 are important for virus infectivity.

Tytuł :
Transcription factor binding sites in the pol gene intragenic regulatory region of HIV-1 are important for virus infectivity.
Index Terms :
Sciences bio-médicales et agricoles
Animals
Binding Sites
Cell Line
DNA Mutational Analysis
DNA-Binding Proteins -- metabolism
Gene Expression Regulation, Viral
Gene Products, pol -- genetics
Gene Products, tat -- metabolism
HIV-1 -- genetics
HIV-1 -- physiology
Humans
Mice
Octamer Transcription Factor-1
Point Mutation
Promoter Regions, Genetic
Proto-Oncogene Proteins -- metabolism
Response Elements
Sp1 Transcription Factor -- metabolism
Sp3 Transcription Factor
Thymidine Kinase -- genetics
Trans-Activators -- metabolism
Transcription Factors -- chemistry
Transcription Factors -- metabolism
Transcriptional Activation
Virus Replication
Zinc Fingers
tat Gene Products, Human Immunodeficiency Virus
info:eu-repo/semantics/article
info:ulb-repo/semantics/articlePeerReview
info:ulb-repo/semantics/openurl/article
Wydawca :
2005
Dodane szczegóły :
Goffin, Véronique
Demonte, Dominique
Vanhulle, Caroline
De Walque, Stéphane
De Launoit, Yvan
Burny, Arsène
Collette, Yves
Van Lint, Carine
Typ dokumentu :
Zasób elektroniczny
URL :
http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/51743">http://hdl.handle.net/2013/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/51743
http://worldcat.org/search?q=on:EQY+http://difusion-oai.ulb.ac.be/oai/request+DCG_ENTIRE_REPOSITORY+CNTCOLL">http://worldcat.org/search?q=on:EQY+http://difusion-oai.ulb.ac.be/oai/request+DCG_ENTIRE_REPOSITORY+CNTCOLL
Dostępność :
Open access content. Open access content
1 full-text file(s): info:eu-repo/semantics/restrictedAccess
Pozostałe numery :
EQY oai:dipot.ulb.ac.be:2013/51743
855619343
Źródło wspomagające :
UNIV LIBR DE BRUXELLES
From OAIster®, provided by the OCLC Cooperative.
Numer akcesji :
edsoai.ocn855619343
Zasób elektroniczny
We have previously identified in the pol gene of human immunodeficiency virus type 1 (HIV-1) a new positive transcriptional regulatory element (nt 4481-4982) containing recognition sites for nuclear proteins (sites B, C, D and a GC-box) [C. Van Lint, J. Ghysdael, P. Paras, Jr, A. Burny and E. Verdin (1994) J. Virol. 68, 2632-2648]. In this study, we have further physically characterized each binding site and have shown that the transcription factors Oct-1, Oct-2, PU.1, Sp1 and Sp3 interact in vitro with the pol region. Chromatin immunoprecipitation assays using HIV-infected cell lines demonstrated in the context of chromatin that Sp1, Sp3, Oct-1 and PU.1 are recruited to the HS7 region in vivo. For each site, we have identified mutations abolishing factor binding to their cognate DNA sequences without altering the underlying amino acid sequence of the integrase. By transient transfection assays, we have demonstrated the involvement of the pol binding sites in the transcriptional enhancing activity of the intragenic region. Our functional results with multimerized wild-type and mutated pol binding sites separately (i.e. in the absence of the other sites) have demonstrated that the PU.1, Sp1, Sp3 and Oct-1 transcription factors regulate the transcriptional activity of a heterologous promoter through their respective HS7 binding sites. Finally, we have investigated the physiological role of the HS7 binding sites in HIV-1 replication and have shown that these sites are important for viral infectivity.
Journal Article
Research Support, Non-U.S. Gov't
info:eu-repo/semantics/published

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