Informacja

Drogi użytkowniku, aplikacja do prawidłowego działania wymaga obsługi JavaScript. Proszę włącz obsługę JavaScript w Twojej przeglądarce.

Przeglądasz jako GOŚĆ
Tytuł pozycji:

Acid-sensing channels in human bladder: expression, function and alterations during bladder pain syndrome.

Tytuł :
Acid-sensing channels in human bladder: expression, function and alterations during bladder pain syndrome.
Index Terms :
Aged; Amiloride/pharmacology; Capsaicin/pharmacology; Cell Line; Cells, Cultured; Cystitis, Interstitial/metabolism; Cystitis, Interstitial/physiopathology; Electrophysiological Phenomena/drug effects; Female; Humans; Immunohistochemistry; Male; Middle Aged; Nerve Tissue Proteins/metabolism; Patch-Clamp Techniques; Sodium Channels/metabolism; TRPV Cation Channels/metabolism; Urinary Bladder/metabolism; Urothelium/metabolism
info:eu-repo/semantics/article
article
Wydawca :
2011
Dodane szczegóły :
Sánchez-Freire, V.
Blanchard, M.G.
Burkhard, F.C.
Kessler, T.M.
Kellenberger, S.
Monastyrskaya, K.
Typ dokumentu :
Zasób elektroniczny
URL :
http://nbn-resolving.org/urn/resolver.pl?urn=urn:nbn:ch:serval-BIB_9046470460E66
Dostępność :
Open access content. Open access content
info:eu-repo/semantics/openAccess
Copying allowed only for non-profit organizations
https://serval.unil.ch/disclaimer
Pozostałe numery :
CHLSR oai:serval.unil.ch:BIB_9046470460E6
https://serval.unil.ch/notice/serval:BIB_9046470460E6
info:pmid:21855903
https://serval.unil.ch/resource/serval:BIB_9046470460E6.P001/REF
urn:nbn:ch:serval-BIB_9046470460E66
1008922569
Źródło wspomagające :
UNIV DE LAUSANNE-LETTRES,HIST,SCI RELG
From OAIster®, provided by the OCLC Cooperative.
Numer akcesji :
edsoai.on1008922569
Zasób elektroniczny
Purpose: To examine the possible role of H+-activated acid-sensing ion channels (ASICs) in pain perception we characterized their expression in bladder dome biopsies of Bladder Pain Syndrome (BPS) patients and controls, in cultured human urothelium and in urothelial TEU-2 cells.Materials and Methods: Cold cut biopsies from the bladder dome were obtained in 8 asymptomatic controls and 28 patients with symptoms of BPS. ASIC expression was analyzed by QPCR and immunofluorescence. The channel function was measured by electrophysiology.Results: ASIC1a, ASIC2a and ASIC3 mRNAs were detected in human bladder. Similar amounts of ASIC1a and -3 were detected in detrusor smooth muscle, whereas in urothelium ASIC3 levels were higher than -1a. ASIC2a mRNA levels were lower than either -1a or -3 in both layers. ASIC currents were measured in TEU-2 cells and in primary cultures of human urothelium, and ASIC expression was confirmed by QPCR. Differentiation of TEU-2 cells caused an up-regulation of ASIC2a and ASIC3, and a down-regulation of ASIC1a mRNAs. BPS patients showed an up-regulation of ASIC2a and -3 mRNA, whereas ASIC1a remained unchanged. In contrast, the mRNA levels of TRPV1 were down-regulated during BPS. All differences were statistically significant (p<0.05)Conclusions: Several different ASIC subunits are expressed in human bladder and TEU-2 cells, where their levels are regulated during urothelial differentiation. An up-regulation of ASIC2a and -3 in BPS suggests their involvement in increased pain and hyperalgesia. A down-regulation of TRPV1 mRNA levels might indicate a different regulatory mechanism, controlling its expression in human bladder.

Ta witryna wykorzystuje pliki cookies do przechowywania informacji na Twoim komputerze. Pliki cookies stosujemy w celu świadczenia usług na najwyższym poziomie, w tym w sposób dostosowany do indywidualnych potrzeb. Korzystanie z witryny bez zmiany ustawień dotyczących cookies oznacza, że będą one zamieszczane w Twoim komputerze. W każdym momencie możesz dokonać zmiany ustawień dotyczących cookies