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Tytuł pozycji:

GABA receptors containing Rdl subunits mediate fast inhibitory synaptic transmission in Drosophila neurons.

Tytuł :
GABA receptors containing Rdl subunits mediate fast inhibitory synaptic transmission in Drosophila neurons.
Index Terms :
Architecture
Life Sciences
Animals
Calcium: metabolism
Calcium Channels: metabolism
Cell Separation
Cells, Cultured
Chloride Channels: metabolism
Chlorides: metabolism
Drosophila
Drosophila Proteins
Embryo, Nonmammalian: innervation
Excitatory Postsynaptic Potentials: drug effects; physiology
GABA Antagonists: pharmacology
Neural Inhibition: drug effects; physiology
Neurons: drug effects; metabolism; physiology
Patch-Clamp Techniques
Picrotoxin: pharmacology
Protein Subunits: metabolism
Receptors, GABA: metabolism
Receptors, GABA-A: metabolism
Synaptic Transmission: drug effects; physiology
Time Factors
gamma-Aminobutyric Acid: metabolism
article
Źródło :
The Journal of neuroscience : the official journal of the Society for Neuroscience vol 23, no 11, 4625-4634; 0270-6474
Wydawca :
eScholarship, University of California 2003-06-01
Dodane szczegóły :
Lee, Daewoo
Su, Hailing
O'Dowd, Diane K
Typ dokumentu :
Zasób elektroniczny
URL :
http://www.escholarship.org/uc/item/7jb943fh">http://www.escholarship.org/uc/item/7jb943fh
Dostępność :
Open access content. Open access content
Attribution (CC BY): http://creativecommons.org/licenses/by/3.0
Pozostałe numery :
CDLER qt7jb943fh
qt7jb943fh
1022158175
Źródło wspomagające :
UC MASS DIGITIZATION
From OAIster®, provided by the OCLC Cooperative.
Numer akcesji :
edsoai.on1022158175
Zasób elektroniczny
GABAergic inhibition in Drosophila, as in other insects and mammals, is important for regulation of activity in the CNS. However, the functional properties of synaptic GABA receptors in Drosophila have not been described. Here, we report that spontaneous GABAergic postsynaptic currents (sPSCs) in cultured embryonic Drosophila neurons are mediated by picrotoxin-sensitive chloride-conducting receptors. A rapid increase in spontaneous firing in response to bath application of picrotoxin demonstrates that these GABA receptors mediate inhibition in the neuronal networks formed in culture. Many of the spontaneous GABAergic synaptic currents are sodium action potential independent [miniature IPSCs (mIPSCs)] but are regulated by external calcium levels. The large variation in mIPSC frequency, amplitude, and kinetics properties between neurons suggests heterogeneity in GABA receptor number, location, and/or subtype. A decrease in the mean mIPSC decay time constant between 2 and 5 d, in the absence of a correlated change in rise time, demonstrates that the functional properties of the synaptic GABA receptors are regulated during maturation in vitro. Finally, neurons from the GABA receptor subunit mutant Rdl exhibit reduced sensitivity to picrotoxin blockade of the mIPSCs and resistance to picrotoxin-induced increases in spontaneous firing frequency. This demonstrates that Rdl containing GABA receptors play a direct role in mediating synaptic inhibition in Drosophila neural circuits formed in culture.

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