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Tytuł pozycji:

Essential roles of G9a in cell proliferation and differentiation during tooth development

Tytuł :
Essential roles of G9a in cell proliferation and differentiation during tooth development
Index Terms :
Animals
Bone Morphogenetic Proteins/metabolism
Cell Differentiation/*physiology
Cell Proliferation/*physiology
Epithelium/metabolism
Fibroblast Growth Factors/metabolism
Gene Expression Regulation, Developmental/*physiology
Histone-Lysine N-Methyltransferase/genetics/*metabolism
Mesoderm/cytology
Mice, Transgenic
Odontogenesis/physiology
Tooth/*growth & development
Transcription Factors/metabolism
Differentiation
Epigenetics
Tooth development
Journal Article
Tytuły dodatkowe :
Essential roles of G9a in cell proliferation and differentiation during tooth development
Wydawca :
2018-01-29
Dodane szczegóły :
Kamiunten, T.
Kamiunten, T.
Ideno, H.
Ideno, H.
Shimada, A.
Shimada, A.
Arai, Y.
Arai, Y.
Terashima, T.
Terashima, T.
Tomooka, Y.
Tomooka, Y.
Nakamura, Y.
Nakamura, Y.
Nakashima, K.
Nakashima, K.
木村, 宏
Kimura, Hiroshi
Shinkai, Y.
Shinkai, Y.
Tachibana, M.
Tachibana, M.
Nifuji, A.
Nifuji, A.
Typ dokumentu :
Zasób elektroniczny
URL :
http://jairo.nii.ac.jp/0083/00264681
10.1016/j.yexcr.2017.05.016
Dostępność :
Open access content. Open access content
Pozostałe numery :
JPNII oai:irdb.nii.ac.jp:0083/00264681
oai:t2r2.star.titech.ac.jp:50402691
0014-4827
Experimental cell research
357
No. 2
202
210
2017-08
1060305037
Źródło wspomagające :
NATIONAL INST OF INFO
From OAIster®, provided by the OCLC Cooperative.
Numer akcesji :
edsoai.on1060305037
Zasób elektroniczny
Teeth develop through interactions between epithelial and mesenchymal tissues mediated by a signaling network comprised of growth factors and transcription factors. However, little is known about how epigenetic modifiers affect signaling pathways and thereby regulate tooth formation. We previously reported that the histone 3 lysine 9 (H3K9) methyltransferase (MTase) G9a is specifically enriched in the tooth mesenchyme during mouse development. In this study, we investigated the functions of G9a in tooth development using G9a conditional knockout (KO) mice. We used Sox9-Cre mice to delete G9a in the tooth mesenchyme because Sox9 is highly expressed in the mesenchyme derived from the cranial neural crest. Immunohistochemical analyses revealed that G9a expression was significantly decreased in the mesenchyme of Sox9-Cre;G9afl/fl (G9a cKO) mice compared with that in Sox9-Cre;G9a fl/+(control) mice. Protein levels of the G9a substrate H3K9me2 were also decreased in the tooth mesenchyme. G9a cKO mice showed smaller tooth germ after embryonic day (E) 16.5 and E17.5, but not at E15.5. The developing cusp tips, which were visible in control mice, were absent in G9a cKO mice at E17.5. At 3 weeks after birth, small first molars with smaller cusps and unseparated roots were formed. Organ culture of tooth germs derived from E15.5 cKO mouse embryos showed impaired tooth development, suggesting that tooth development per se is affected independently of skull development. BrdU labeling experiments revealed that the proliferation rates were decreased in the mesenchyme in G9a cKO mice at E17.5. In addition, the proliferation rates in the tooth inner enamel epithelium were also decreased. In situ hybridization revealed altered localization of genes associated with tooth development. In cKO mice, intensively localized expression of mRNAs encoding bone morphogenic protein (Bmp2 and Bmp4) was observed in the tooth mesenchyme at E17.5, similar to the expression patterns observed in contro

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