High-resolution, multicolor 3D imaging at the speed of life.

The article reports on a study that uses swept confocally aligned planar excitation microscopy at Columbia University to overcome the limitations of earlier methods and offer broad life sciences utility. Highlights include the use of an off-axis path through the edge of a high numerical-aperture objective, how the problem of imaging a sloped plane is addressed, and the innovative advantages of optically-pumped semiconductor laser technology for the study.