Elevated expression of tumour necrosis factor-α-induced protein 8 (TNFAIP8)-like 2 m RNA in peripheral blood mononuclear cells is associated with disease progression of acute-on-chronic hepatitis B liver failure.

Aberrant immunity response contributes to the pathogenesis of acute-on-chronic hepatitis B liver failure. Tumour necrosis factor-α-induced protein-8 like-2 ( TIPE2) is a recently identified molecular to maintain immune homoeostasis, but its role in acute-on-chronic hepatitis B liver failure ( ACHBLF) is unknown. We detected TIPE2 m RNA levels in peripheral blood mononuclear cells ( PBMCs) from 56 patients with ACHBLF, 60 chronic hepatitis B patients, 24 healthy controls and analysed its role in disease severity and prognosis. TIPE2 m RNA expression in patients with ACHBLF was higher than that of patients with chronic infection or healthy controls. In patients with ACHBLF, TIPE2 m RNA level was positively correlated with serum total bilirubin, international normalized ratio and model for end-stage liver disease scores. Furthermore, the level of TIPE2 m RNA was significantly higher in nonsurvivors than survivors in patients with ACHBLF. The m RNA level of TIPE2 gradually decreased week by week in survivors accompanied by recovery from patients with ACHBLF, while its expression sustained at high levels in nonsurvivors. TIPE2 m RNA level after lipopolysaccharide ( LPS) stimulation ex vivo in patients with ACHBLF was higher compared with controls and patients with chronic infection. Meanwhile, cytokines ex vivo secreted were measured as a marker of immune activation. After LPS stimulation, interleukin (IL)-6 and tumour necrosis factor (TNF)-α m RNA expression were reduced in patients with ACHBLF, and a significantly negative correlation was found between TIPE2 and TNF-α m RNA levels. In conclusion, our results identified the potential role of TIPE2 in predicting disease progression and prognosis in patients with ACHBLF by negative regulating of cell-mediated immunity. [ABSTRACT FROM AUTHOR]
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