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Wyszukujesz frazę ""Bioluminescence Resonance Energy Transfer Techniques"" wg kryterium: Temat


Tytuł :
A synthetic BRET-based optogenetic device for pulsatile transgene expression enabling glucose homeostasis in mice.
Autorzy :
Li T; Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.; School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
Chen X; Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.; School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.; CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, 200031, China.
Qian Y; Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.; School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
Shao J; Synthetic Biology and Biomedical Engineering Laboratory, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China.
Li X; Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.; School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
Liu S; Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.; School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
Zhu L; Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
Zhao Y; Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.; School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China.
Ye H; Synthetic Biology and Biomedical Engineering Laboratory, Biomedical Synthetic Biology Research Center, Shanghai Key Laboratory of Regulatory Biology, Institute of Biomedical Sciences and School of Life Sciences, East China Normal University, Dongchuan Road 500, Shanghai, 200241, China. .
Yang Y; Optogenetics & Synthetic Biology Interdisciplinary Research Center, State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Center for Biomanufacturing Technology, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China. .; School of Pharmacy, East China University of Science and Technology, 130 Mei Long Road, Shanghai, 200237, China. .; CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai, 200031, China. .
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Źródło :
Nature communications [Nat Commun] 2021 Jan 27; Vol. 12 (1), pp. 615. Date of Electronic Publication: 2021 Jan 27.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques*
Gene Expression*
Homeostasis*
Optogenetics*
Transgenes*
Blood Glucose/*metabolism
Animals ; Cell Line ; Diabetes Mellitus, Experimental/blood ; Diabetes Mellitus, Type 1/blood ; Humans ; Kinetics ; Mice, Inbred C57BL ; Time Factors
Czasopismo naukowe
Tytuł :
Development and characterisation of a compact device for rapid real-time-on-chip detection of thrombin activity in human serum using bioluminescence resonance energy transfer (BRET).
Autorzy :
Weihs F; CSIRO Health & Biosecurity, Parkville, 343 Royal Parade, Melbourne, VIC, 3030, Australia. Electronic address: .
Gel M; CSIRO Manufacturing, Clayton, VIC, 3168, Australia.
Wang J; CSIRO Health & Biosecurity, Canberra, ACT, 2601, Australia.
Anderson A; CSIRO Health & Biosecurity, Canberra, ACT, 2601, Australia.
Trowell S; CSIRO Health & Biosecurity, Canberra, ACT, 2601, Australia.
Dacres H; CSIRO Health & Biosecurity, Food Innovation Centre, 671 Sneydes Road, Werribee, VIC, 3030, Australia.
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Źródło :
Biosensors & bioelectronics [Biosens Bioelectron] 2020 Jun 15; Vol. 158, pp. 112162. Date of Electronic Publication: 2020 Mar 23.
Typ publikacji :
Journal Article
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques*
Biosensing Techniques*
Lab-On-A-Chip Devices*
Thrombin*/metabolism
Biomarkers/*blood
Luminescent Measurements/*instrumentation
Luminescent Measurements/*methods
Equipment Design ; Genes, Reporter ; Humans ; Luminescent Proteins ; Point-of-Care Testing ; Reproducibility of Results ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Noncanonical scaffolding of G αi and β-arrestin by G protein-coupled receptors.
Autorzy :
Smith JS; Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Pack TF; Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.; Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710, USA.
Inoue A; Department of Pharmaceutical Sciences, Tohoku University, Japan.
Lee C; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Zheng K; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Choi I; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Eiger DS; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Warman A; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Xiong X; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Ma Z; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Viswanathan G; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Levitan IM; Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.
Rochelle LK; Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.; Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA.
Staus DP; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Snyder JC; Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.; Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA.
Kahsai AW; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
Caron MG; Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.; Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, USA.; Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA.
Rajagopal S; Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA. .; Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
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Źródło :
Science (New York, N.Y.) [Science] 2021 Mar 12; Vol. 371 (6534). Date of Electronic Publication: 2021 Jan 21.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
GTP-Binding Protein alpha Subunits, Gi-Go/*metabolism
Receptors, G-Protein-Coupled/*metabolism
beta-Arrestins/*metabolism
Bioluminescence Resonance Energy Transfer Techniques ; Cell Movement ; Extracellular Signal-Regulated MAP Kinases/metabolism ; HEK293 Cells ; Humans ; Signal Transduction
Czasopismo naukowe
Tytuł :
An improved fluorescent protein-based expression reporter system that utilizes bioluminescence resonance energy transfer and peptide-assisted complementation.
Autorzy :
Kakizuka T; Laboratory for Comprehensive Bioimaging, RIKEN Center for Biosystems Dynamics Research (BDR), 6-2-3 Furuedai, Suita-Shi, Osaka 565-0874, Japan. .
Takai A
Yoshizawa K
Okada Y
Watanabe TM
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Źródło :
Chemical communications (Cambridge, England) [Chem Commun (Camb)] 2020 Mar 28; Vol. 56 (25), pp. 3625-3628. Date of Electronic Publication: 2020 Feb 27.
Typ publikacji :
Journal Article
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques*
Bacterial Proteins/*genetics
Genes, Reporter/*genetics
Luminescent Proteins/*genetics
Peptides/*metabolism
Bacterial Proteins/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Humans ; Luminescent Measurements ; Luminescent Proteins/metabolism
Czasopismo naukowe
Tytuł :
Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells.
Autorzy :
Tsuboi S; Riken Center for Biosystems Dynamics Research, Riken, Osaka, Japan.
Jin T; Riken Center for Biosystems Dynamics Research, Riken, Osaka, Japan. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2081, pp. 15-27.
Typ publikacji :
Journal Article
MeSH Terms :
Apoptosis*
Bioluminescence Resonance Energy Transfer Techniques*
Molecular Imaging*/methods
Flow Cytometry ; Glutathione ; Humans ; Luciferases, Renilla/genetics ; Luciferases, Renilla/metabolism ; Luminescent Measurements/methods ; Optical Imaging/methods ; Quantum Dots
Czasopismo naukowe
Tytuł :
Portable and Field-Ready Detection of Circulating MicroRNAs with Paper-Based Bioluminescent Sensing and Isothermal Amplification.
Autorzy :
Li Y
Zhou L
Ni W; Hubei Provincial Hospital of Traditional Chinese Medicine , Hubei Province Academy of Traditional Chinese Medicine , Wuhan 430061 , P. R. China.
Luo Q; Research Center for Micro/Nano System & Bionic Medicine, Institute of Biomedical & Health Engineering , Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences , Shenzhen 518055 , P. R. China.
Zhu C
Wu Y
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Źródło :
Analytical chemistry [Anal Chem] 2019 Dec 03; Vol. 91 (23), pp. 14838-14841. Date of Electronic Publication: 2019 Nov 11.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques*
Carcinoma, Non-Small-Cell Lung/*diagnosis
Circulating MicroRNA/*genetics
Lung Neoplasms/*diagnosis
MicroRNAs/*genetics
RNA, Neoplasm/*genetics
Carcinoma, Non-Small-Cell Lung/blood ; Carcinoma, Non-Small-Cell Lung/genetics ; Circulating MicroRNA/blood ; Humans ; Lung Neoplasms/blood ; Lung Neoplasms/genetics ; MicroRNAs/blood ; Nucleic Acid Amplification Techniques ; Paper ; RNA, Neoplasm/blood ; Reagent Strips/analysis ; Smartphone
Czasopismo naukowe
Tytuł :
Heteromeric complex formation between human cytochrome P450 CYP1A1 and heme oxygenase-1.
Autorzy :
Connick JP; Department of Pharmacology and Experimental Therapeutics, and the Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA, U.S.A.
Reed JR; Department of Pharmacology and Experimental Therapeutics, and the Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA, U.S.A.
Cawley GF; Department of Pharmacology and Experimental Therapeutics, and the Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA, U.S.A.
Backes WL; Department of Pharmacology and Experimental Therapeutics, and the Stanley S. Scott Cancer Center, Louisiana State University Health Sciences Center, New Orleans, LA, U.S.A.
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Źródło :
The Biochemical journal [Biochem J] 2021 Jan 29; Vol. 478 (2), pp. 377-388.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Cytochrome P-450 CYP1A1/*metabolism
Heme Oxygenase-1/*metabolism
Bioluminescence Resonance Energy Transfer Techniques ; Cytochrome P-450 CYP1A1/chemistry ; Cytochrome P-450 Enzyme System/chemistry ; Cytochrome P-450 Enzyme System/metabolism ; Heme Oxygenase-1/chemistry ; Humans ; Protein Interaction Domains and Motifs
Czasopismo naukowe
Tytuł :
Recent advances in visual detection for cancer biomarkers and infectious pathogens.
Autorzy :
Pan MM; Tongji School of Pharmacy, HuaZhong University of Science and Technology, Wuhan 430030, China. .
Wang YF
Wang L
Yu X
Xu L
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Źródło :
Journal of materials chemistry. B [J Mater Chem B] 2021 Jan 07; Vol. 9 (1), pp. 35-52. Date of Electronic Publication: 2020 Nov 23.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques/*methods
Biomarkers, Tumor/*metabolism
Biosensing Techniques/*methods
Neoplasms/*metabolism
Animals ; Biomarkers, Tumor/genetics ; CRISPR-Cas Systems/physiology ; Colorimetry/methods ; Humans ; Neoplasms/diagnosis ; Surface Plasmon Resonance/methods
Czasopismo naukowe
Tytuł :
Monitoring Opioid Receptor Interaction in Living Cells by Bioluminescence Resonance Energy Transfer (BRET).
Autorzy :
Baiula M; Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Bologna, Italy. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2201, pp. 35-43.
Typ publikacji :
Journal Article
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques/*methods
Receptors, Opioid, mu/*metabolism
Animals ; Carrier Proteins/metabolism ; Cell Line ; Cell Membrane/metabolism ; Energy Transfer ; Fluorescence ; Fluorescence Resonance Energy Transfer/methods ; Humans ; Luciferases, Renilla/metabolism ; Luminescent Measurements ; Signal Transduction/drug effects
Czasopismo naukowe
Tytuł :
Bioluminescence Resonance Energy Transfer (BRET) to Detect the Interactions Between Kappa Opioid Receptor and Nonvisual Arrestins.
Autorzy :
Bedini A; Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Bologna, Italy. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2201, pp. 45-58.
Typ publikacji :
Journal Article
MeSH Terms :
Arrestins/*metabolism
Bioluminescence Resonance Energy Transfer Techniques/*methods
Receptors, Opioid, kappa/*metabolism
Animals ; Cell Line ; Cell Membrane/metabolism ; Energy Transfer ; Fluorescence ; Fluorescence Resonance Energy Transfer/methods ; HEK293 Cells ; Humans ; Ligands ; Luciferases, Renilla/metabolism ; Luminescent Measurements ; Protein Binding ; Receptors, G-Protein-Coupled/metabolism ; beta-Arrestin 1/metabolism ; beta-Arrestin 2/metabolism ; beta-Arrestins/metabolism
Czasopismo naukowe
Tytuł :
Evaluating Opioid-Mediated Adenylyl Cyclase Inhibition in Live Cells Using a BRET-Based Assay.
Autorzy :
Manandhar P; Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.
Sachdev S; Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia.
Santiago M; Department of Biomedical Sciences, Faculty of Medicine, Health and Human Sciences, Macquarie University, Sydney, NSW, Australia. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2201, pp. 117-125.
Typ publikacji :
Journal Article
MeSH Terms :
Adenylyl Cyclase Inhibitors/*analysis
Bioluminescence Resonance Energy Transfer Techniques/*methods
Molecular Imaging/*methods
Adenylate Cyclase Toxin ; Adenylyl Cyclase Inhibitors/metabolism ; Adenylyl Cyclases/metabolism ; Analgesics, Opioid ; Animals ; Colforsin/pharmacology ; Cyclic AMP ; Energy Transfer ; HEK293 Cells ; Humans ; Receptors, Opioid/chemistry ; Receptors, Opioid/metabolism ; Receptors, Opioid, mu
Czasopismo naukowe
Tytuł :
The GTPase-activating protein-related domain of neurofibromin interacts with MC1R and regulates pigmentation-mediated signaling in human melanocytes.
Autorzy :
Deraredj Nadim W; Centre de Biophysique Moléculaire, CNRS UPR4301 Affiliated to the University of Orléans, Rue Charles Sadron, 45071, Orléans, Cedex 2, France.
Hassanaly S; Centre de Biophysique Moléculaire, CNRS UPR4301 Affiliated to the University of Orléans, Rue Charles Sadron, 45071, Orléans, Cedex 2, France.
Bénédetti H; Centre de Biophysique Moléculaire, CNRS UPR4301 Affiliated to the University of Orléans, Rue Charles Sadron, 45071, Orléans, Cedex 2, France.
Kieda C; Centre de Biophysique Moléculaire, CNRS UPR4301 Affiliated to the University of Orléans, Rue Charles Sadron, 45071, Orléans, Cedex 2, France.
Grillon C; Centre de Biophysique Moléculaire, CNRS UPR4301 Affiliated to the University of Orléans, Rue Charles Sadron, 45071, Orléans, Cedex 2, France.
Morisset-Lopez S; Centre de Biophysique Moléculaire, CNRS UPR4301 Affiliated to the University of Orléans, Rue Charles Sadron, 45071, Orléans, Cedex 2, France. Electronic address: .
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Źródło :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Jan 01; Vol. 534, pp. 758-764. Date of Electronic Publication: 2020 Nov 10.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Melanocytes/*metabolism
Neurofibromin 1/*metabolism
Receptor, Melanocortin, Type 1/*metabolism
Bioluminescence Resonance Energy Transfer Techniques ; GTPase-Activating Proteins/metabolism ; HEK293 Cells ; Humans ; Melanins/metabolism ; Mutation ; Neurofibromatosis 1/genetics ; Neurofibromin 1/genetics ; Pigmentation/physiology ; Protein Interaction Domains and Motifs ; Signal Transduction/physiology
Czasopismo naukowe
Tytuł :
Innovative Bioluminescence Resonance Energy Transfer Assay Reveals Differential Agonist-Induced D2 Receptor Intracellular Trafficking and Arrestin-3 Recruitment.
Autorzy :
De Vries L; Central Nervous System Innovation Unit, CEPC Campans - Belair de Campans, Castres, France (L.D.V., F.F., C.C., D.C.) and CNRS, UMR5547, Centre de Biologie du Développement, Université de Toulouse III-Paul Sabatier, Toulouse, France (B.R.) .
Finana F; Central Nervous System Innovation Unit, CEPC Campans - Belair de Campans, Castres, France (L.D.V., F.F., C.C., D.C.) and CNRS, UMR5547, Centre de Biologie du Développement, Université de Toulouse III-Paul Sabatier, Toulouse, France (B.R.).
Cathala C; Central Nervous System Innovation Unit, CEPC Campans - Belair de Campans, Castres, France (L.D.V., F.F., C.C., D.C.) and CNRS, UMR5547, Centre de Biologie du Développement, Université de Toulouse III-Paul Sabatier, Toulouse, France (B.R.).
Ronsin B; Central Nervous System Innovation Unit, CEPC Campans - Belair de Campans, Castres, France (L.D.V., F.F., C.C., D.C.) and CNRS, UMR5547, Centre de Biologie du Développement, Université de Toulouse III-Paul Sabatier, Toulouse, France (B.R.).
Cussac D; Central Nervous System Innovation Unit, CEPC Campans - Belair de Campans, Castres, France (L.D.V., F.F., C.C., D.C.) and CNRS, UMR5547, Centre de Biologie du Développement, Université de Toulouse III-Paul Sabatier, Toulouse, France (B.R.).
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Źródło :
Molecular pharmacology [Mol Pharmacol] 2019 Sep; Vol. 96 (3), pp. 308-319. Date of Electronic Publication: 2019 Jul 02.
Typ publikacji :
Journal Article
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques*
Arrestins/*metabolism
Green Fluorescent Proteins/*metabolism
Receptors, Dopamine D2/*agonists
Animals ; CHO Cells ; Cricetulus ; Dopamine Antagonists/pharmacology ; Haloperidol/pharmacology ; Lysosomes/metabolism ; Proto-Oncogene Proteins p21(ras)/metabolism ; Signal Transduction ; rab GTP-Binding Proteins/metabolism
Czasopismo naukowe
Tytuł :
Comparison of the ligand-binding properties of fluorescent VEGF-A isoforms to VEGF receptor 2 in living cells and membrane preparations using NanoBRET.
Autorzy :
Peach CJ; Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.; Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, The Midlands, UK.
Kilpatrick LE; Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.; Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, The Midlands, UK.
Woolard J; Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.; Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, The Midlands, UK.
Hill SJ; Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK.; Centre of Membrane Proteins and Receptors, University of Birmingham and University of Nottingham, The Midlands, UK.
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Źródło :
British journal of pharmacology [Br J Pharmacol] 2019 Sep; Vol. 176 (17), pp. 3220-3235. Date of Electronic Publication: 2019 Jul 15.
Typ publikacji :
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques*
Fluorescence*
Protein Kinase Inhibitors/*pharmacology
Quinazolines/*pharmacology
Vascular Endothelial Growth Factor A/*antagonists & inhibitors
Vascular Endothelial Growth Factor Receptor-2/*antagonists & inhibitors
Binding Sites/drug effects ; Cell Membrane/drug effects ; Cell Membrane/metabolism ; Cells, Cultured ; Dose-Response Relationship, Drug ; Endosomes/drug effects ; Endosomes/metabolism ; HEK293 Cells ; Humans ; Ligands ; Protein Isoforms/drug effects ; Protein Kinase Inhibitors/chemistry ; Quinazolines/chemistry ; Structure-Activity Relationship ; Vascular Endothelial Growth Factor A/metabolism ; Vascular Endothelial Growth Factor Receptor-2/metabolism
Czasopismo naukowe
Tytuł :
In vivo imaging of long-term accumulation of cancer-derived exosomes using a BRET-based reporter.
Autorzy :
Hikita T; Division of Cancer Cell Regulation, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, 464-8681, Japan.
Miyata M; Division of Cancer Cell Regulation, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, 464-8681, Japan.
Watanabe R; Division of Cancer Cell Regulation, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, 464-8681, Japan.
Oneyama C; Division of Cancer Cell Regulation, Aichi Cancer Center Research Institute, Chikusa-ku, Nagoya, 464-8681, Japan. .; Department of Target and Drug Discovery, Graduate School of Medicine, Nagoya University, Showa-ku, Nagoya, Japan. .; Department of Oncology, Graduate School of Pharmaceutical Sciences, Nagoya City University, Mizuho-ku, Nagoya, Japan. .; JST, PRESTO, Nagoya, Japan. .
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Źródło :
Scientific reports [Sci Rep] 2020 Oct 06; Vol. 10 (1), pp. 16616. Date of Electronic Publication: 2020 Oct 06.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Energy Transfer*
Bioluminescence Resonance Energy Transfer Techniques/*methods
Exosomes/*metabolism
Molecular Imaging/*methods
Prostatic Neoplasms/*metabolism
Prostatic Neoplasms/*pathology
Animals ; Dasatinib/pharmacology ; Heterografts ; Male ; Mice ; Neoplasm Transplantation ; Protein Kinase Inhibitors/pharmacology ; Time Factors
Czasopismo naukowe
Tytuł :
Unique Pharmacological Properties of the Kappa Opioid Receptor Signaling Through G α z as Shown with Bioluminescence Resonance Energy Tranfer.
Autorzy :
Barnett ME; Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry, Rochester, New York.
Knapp BI; Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry, Rochester, New York.
Bidlack JM; Department of Pharmacology and Physiology, University of Rochester, School of Medicine and Dentistry, Rochester, New York .
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Źródło :
Molecular pharmacology [Mol Pharmacol] 2020 Oct; Vol. 98 (4), pp. 462-474. Date of Electronic Publication: 2020 Jul 27.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Analgesics, Opioid/*pharmacology
Bioluminescence Resonance Energy Transfer Techniques/*methods
GTP-Binding Protein alpha Subunits/*metabolism
Receptors, Opioid, kappa/*metabolism
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer/pharmacology ; Diterpenes, Clerodane/pharmacology ; Dynorphins/pharmacology ; HEK293 Cells ; Humans ; Morphinans/pharmacology ; Signal Transduction/drug effects ; Spiro Compounds/pharmacology
Czasopismo naukowe
Tytuł :
Agonist-induced formation of unproductive receptor-G 12 complexes.
Autorzy :
Okashah N; Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, GA 30912.
Wright SC; Institute for Research in Immunology and Cancer, Department of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC H3T 1J4, Canada.
Kawakami K; Graduate School of Pharmaceutical Sciences, Tohoku University, 980-8578 Sendai, Japan.
Mathiasen S; Department of Psychiatry, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032.; Department of Pharmacology, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032.; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032.
Zhou J; Institute for Research in Immunology and Cancer, Department of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC H3T 1J4, Canada.
Lu S; Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, GA 30912.
Javitch JA; Department of Psychiatry, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032.; Department of Pharmacology, Columbia University Vagelos College of Physicians and Surgeons, New York, NY 10032.; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032.
Inoue A; Graduate School of Pharmaceutical Sciences, Tohoku University, 980-8578 Sendai, Japan.
Bouvier M; Institute for Research in Immunology and Cancer, Department of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC H3T 1J4, Canada.
Lambert NA; Department of Pharmacology and Toxicology, Medical College of Georgia, Augusta University, Augusta, GA 30912; .
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Źródło :
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2020 Sep 01; Vol. 117 (35), pp. 21723-21730. Date of Electronic Publication: 2020 Aug 17.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
GTP-Binding Protein alpha Subunits, G12-G13/*metabolism
Receptors, G-Protein-Coupled/*agonists
Receptors, G-Protein-Coupled/*metabolism
Bioluminescence Resonance Energy Transfer Techniques/methods ; Cyclic AMP/metabolism ; GTP-Binding Protein alpha Subunits, G12-G13/physiology ; GTP-Binding Protein alpha Subunits, Gs/metabolism ; GTP-Binding Protein alpha Subunits, Gs/physiology ; GTP-Binding Proteins/metabolism ; HEK293 Cells ; Humans ; Ligands ; Protein Binding/physiology ; Receptors, Vasopressin/metabolism ; Signal Transduction/physiology ; Vasopressins/metabolism ; beta-Arrestins/metabolism
Czasopismo naukowe
Tytuł :
Bioluminescence Resonance Energy Transfer as a Method to Study Protein-Protein Interactions: Application to G Protein Coupled Receptor Biology.
Autorzy :
El Khamlichi C; Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM, 45071 Orléans, France. .; PRC, INRA, CNRS, Université François Rabelais-Tours, 37380 Nouzilly, France. .
Reverchon-Assadi F; Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM, 45071 Orléans, France. .
Hervouet-Coste N; Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM, 45071 Orléans, France. .
Blot L; Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM, 45071 Orléans, France. .
Reiter E; PRC, INRA, CNRS, Université François Rabelais-Tours, 37380 Nouzilly, France. .
Morisset-Lopez S; Centre de Biophysique Moléculaire, CNRS, UPR 4301, University of Orléans and INSERM, 45071 Orléans, France. .
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Źródło :
Molecules (Basel, Switzerland) [Molecules] 2019 Feb 01; Vol. 24 (3). Date of Electronic Publication: 2019 Feb 01.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques*
High-Throughput Screening Assays*
Protein Interaction Domains and Motifs/*drug effects
Receptors, G-Protein-Coupled/*metabolism
Small Molecule Libraries/*pharmacology
Antibodies/chemistry ; Antibodies/pharmacology ; Aptamers, Nucleotide/chemical synthesis ; Aptamers, Nucleotide/pharmacology ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Carbocyanines/chemistry ; Carbocyanines/metabolism ; Genes, Reporter ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Humans ; Luciferases/genetics ; Luciferases/metabolism ; Luminescent Proteins/genetics ; Luminescent Proteins/metabolism ; Peptidomimetics/chemical synthesis ; Peptidomimetics/pharmacology ; Protein Multimerization ; Quantum Dots/chemistry ; Quantum Dots/metabolism ; Receptors, G-Protein-Coupled/agonists ; Receptors, G-Protein-Coupled/antagonists & inhibitors ; Receptors, G-Protein-Coupled/genetics ; Signal Transduction/drug effects ; Small Molecule Libraries/chemical synthesis
Czasopismo naukowe
Tytuł :
Development of Nonspecific BRET-Based Biosensors to Monitor Plasma Membrane Inositol Lipids in Living Cells.
Autorzy :
Tóth JT; Faculty of Medicine, Department of Physiology, Semmelweis University, Budapest, Hungary.; Faculty of Medicine, Department of Anesthesiology and Intensive Therapy, Semmelweis University, Budapest, Hungary.
Gulyás G; Faculty of Medicine, Department of Physiology Semmelweis University, Budapest, Hungary.
Hunyady L; Faculty of Medicine, Department of Physiology, Semmelweis University, Budapest, Hungary.; MTA-SE Laboratory of Molecular Physiology, Budapest, Hungary.
Várnai P; Faculty of Medicine, Department of Physiology, Semmelweis University, Budapest, Hungary. .; MTA-SE Laboratory of Molecular Physiology, Budapest, Hungary. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1949, pp. 23-34.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques*
Biosensing Techniques*
Cell Membrane/*metabolism
Inositol/*metabolism
Animals ; Cell Line ; Data Analysis ; Humans ; Lipid Metabolism
Czasopismo naukowe
Tytuł :
Monitoring Non-vesicular Transport of Phosphatidylserine and Phosphatidylinositol 4-Phosphate in Intact Cells by BRET Analysis.
Autorzy :
Sohn M; Section on Molecular Signal Transduction, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Toth DJ; Section on Molecular Signal Transduction, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA.
Balla T; Section on Molecular Signal Transduction, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1949, pp. 13-22.
Typ publikacji :
Journal Article; Research Support, N.I.H., Intramural
MeSH Terms :
Bioluminescence Resonance Energy Transfer Techniques*
Phosphatidylinositol Phosphates/*metabolism
Phosphatidylserines/*metabolism
Biological Transport ; Carrier Proteins/metabolism ; Cell Membrane/metabolism ; Humans ; Lipid Metabolism
Czasopismo naukowe

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