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Wyszukujesz frazę ""Blotting, Northern"" wg kryterium: Temat


Tytuł :
Empirical Validation of Overlapping Virus lncRNAs and Coding Transcripts by Northern Blot.
Autorzy :
Kara M; Department of Molecular Biology and Genetics, College of Arts and Sciences, Uludağ University, Bursa, Turkey.; Department of Molecular Genetics & Microbiology, College of Medicine, UF Health Cancer Center, University of Florida, Gainesville, FL, USA.
Tibbetts SA; Department of Molecular Genetics & Microbiology, College of Medicine, UF Health Cancer Center, University of Florida, Gainesville, FL, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2348, pp. 243-253.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Blotting, Northern*/methods
Genes, Overlapping*
RNA, Long Noncoding/*genetics
RNA, Messenger/*genetics
RNA, Viral/*genetics
Electrophoresis, Polyacrylamide Gel ; Gene Expression Regulation, Viral ; Genome, Viral ; Herpesviridae/genetics ; Open Reading Frames ; Viruses/genetics
Czasopismo naukowe
Tytuł :
Visualizing RNA Cytidine Acetyltransferase Activity by Northern Blotting.
Autorzy :
Bryson KM; Chemical Biology Laboratory, National Cancer Institute, Frederick, Maryland.
Thalalla-Gamage S; Chemical Biology Laboratory, National Cancer Institute, Frederick, Maryland.
Meier JL; Chemical Biology Laboratory, National Cancer Institute, Frederick, Maryland.
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Źródło :
Current protocols in chemical biology [Curr Protoc Chem Biol] 2020 Dec; Vol. 12 (4), pp. e89.
Typ publikacji :
Journal Article; Research Support, N.I.H., Intramural
MeSH Terms :
Blotting, Northern*
Acetyltransferases/*analysis
Cytidine/*analysis
RNA/*chemistry
Acetyltransferases/metabolism ; Cytidine/metabolism ; Humans ; RNA/metabolism
Czasopismo naukowe
Tytuł :
Selectivity of mRNA degradation by autophagy in yeast.
Autorzy :
Makino S; Cell Biology Center, Tokyo Institute of Technology, Yokohama, Japan.
Kawamata T; Cell Biology Center, Tokyo Institute of Technology, Yokohama, Japan.
Iwasaki S; RNA Systems Biochemistry Laboratory, RIKEN Cluster for Pioneering Research, Saitama, Japan. .; Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Chiba, Japan. .; AMED-CREST, Japan Agency for Medical Research and Development, Saitama, Japan. .
Ohsumi Y; Cell Biology Center, Tokyo Institute of Technology, Yokohama, Japan. .
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Źródło :
Nature communications [Nat Commun] 2021 Apr 19; Vol. 12 (1), pp. 2316. Date of Electronic Publication: 2021 Apr 19.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Autophagy/*genetics
RNA Stability/*genetics
Ribosomes/*genetics
Saccharomyces cerevisiae/*genetics
Vacuoles/*genetics
Blotting, Northern ; RNA, Fungal/genetics ; RNA, Fungal/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA-Seq/methods ; Reverse Transcriptase Polymerase Chain Reaction ; Ribosomal Proteins/genetics ; Ribosomal Proteins/metabolism ; Ribosomes/metabolism ; Saccharomyces cerevisiae/metabolism ; Vacuoles/metabolism
Czasopismo naukowe
Tytuł :
[Differentially Expressed Long Noncoding RNAs in the Promoter Region of the fork head Gene in Drosophila melanogaster Detected by Northern Blot Hybridization].
Autorzy :
Burlin AI; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
Tillib SV; Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.; .
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Źródło :
Molekuliarnaia biologiia [Mol Biol (Mosk)] 2019 May-Jun; Vol. 53 (3), pp. 476-484.
Typ publikacji :
Journal Article
MeSH Terms :
Blotting, Northern*
Gene Expression Regulation, Developmental*
Drosophila Proteins/*genetics
Drosophila melanogaster/*genetics
Forkhead Transcription Factors/*genetics
Promoter Regions, Genetic/*genetics
RNA, Long Noncoding/*analysis
RNA, Long Noncoding/*genetics
Animals ; Cell Line ; Drosophila melanogaster/embryology ; Drosophila melanogaster/growth & development ; Enhancer Elements, Genetic/genetics ; Female ; Male ; Organ Specificity
Czasopismo naukowe
Tytuł :
MicroRNA Detection Specificity: Recent Advances and Future Perspective.
Autorzy :
Ouyang T; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering , Southeast University , Nanjing 210096 , China.
Liu Z; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering , Southeast University , Nanjing 210096 , China.
Han Z; Department of Liver Diseases , Shenzhen Traditional Chinese Medicine Hospital , Shenzhen 518033 , China.
Ge Q; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering , Southeast University , Nanjing 210096 , China.
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Źródło :
Analytical chemistry [Anal Chem] 2019 Mar 05; Vol. 91 (5), pp. 3179-3186. Date of Electronic Publication: 2019 Feb 15.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Blotting, Northern*
Reverse Transcriptase Polymerase Chain Reaction*
High-Throughput Nucleotide Sequencing/*methods
MicroRNAs/*analysis
Oligonucleotide Array Sequence Analysis/*methods
DNA Ligases/metabolism ; Humans ; MicroRNAs/chemistry ; Nucleic Acid Conformation ; Sequence Analysis, RNA
Czasopismo naukowe
Tytuł :
Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs.
Autorzy :
Bhardwaj AR; Department of Botany, Ramjas College, University of Delhi North Campus, Delhi, India.
Pandey R; Department of Botany, SGTB Khalsa College, University of Delhi North Campus, Delhi, India.
Agarwal M; Department of Botany, University of Delhi North Campus, Delhi, India. .
Katiyar-Agarwal S; Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, India. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2170, pp. 155-183.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Blotting, Northern/*methods
RNA, Messenger/*genetics
RNA, Small Untranslated/genetics
Czasopismo naukowe
Tytuł :
Techniques for Characterizing Cytomegalovirus-Encoded miRNAs.
Autorzy :
Diggins NL; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Crawford LB; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Struthers HM; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Hook LM; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Landais I; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Skalsky RL; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA.
Hancock MH; Vaccine and Gene Therapy Institute, Oregon Health and Sciences University, Beaverton, OR, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2244, pp. 301-342.
Typ publikacji :
Journal Article
MeSH Terms :
Cytomegalovirus/*genetics
Immunoprecipitation/*methods
MicroRNAs/*analysis
3' Untranslated Regions/genetics ; Blotting, Northern/methods ; Gene Expression/genetics ; Gene Expression Regulation, Viral/genetics ; Humans ; MicroRNAs/genetics ; RNA, Messenger/genetics
Czasopismo naukowe
Tytuł :
Study of Circular RNA Expression by Nonradioactive Northern Blot Procedure.
Autorzy :
D'Ambra E; Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Rome, Italy.
Morlando M; Department of Pharmaceutical Sciences, 'Department of Excellence 2018-2022', University of Perugia, Perugia, Italy. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2348, pp. 371-383.
Typ publikacji :
Journal Article
MeSH Terms :
Gene Expression*
RNA, Circular*
Blotting, Northern/*methods
Humans ; RNA Probes ; RNA, Untranslated
Czasopismo naukowe
Tytuł :
Design and Evaluation of Guide RNA Transcripts with a 3'-Terminal HDV Ribozyme to Enhance CRISPR-Based Gene Inactivation.
Autorzy :
Berkhout B; Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. .
Gao Z; Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
Herrera-Carrillo E; Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2167, pp. 205-224.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
CRISPR-Cas Systems*
Bacterial Proteins/*metabolism
CRISPR-Associated Proteins/*metabolism
Endodeoxyribonucleases/*metabolism
Gene Editing/*methods
Hepatitis Delta Virus/*genetics
Hepatitis Delta Virus/*metabolism
RNA, Catalytic/*metabolism
RNA, Guide/*genetics
Bacterial Proteins/genetics ; Blotting, Northern ; Endonucleases/genetics ; Endonucleases/metabolism ; Enzyme Assays/methods ; Flow Cytometry ; Gene Knockout Techniques ; Gene Silencing ; Genes, Reporter ; Genetic Vectors ; HEK293 Cells ; HeLa Cells ; Humans ; INDEL Mutation ; Luciferases ; RNA, Catalytic/genetics ; RNA, Guide/metabolism ; Receptors, CCR5/genetics
Czasopismo naukowe
Tytuł :
Analysis of Queuosine tRNA Modification Using APB Northern Blot Assay.
Autorzy :
Cirzi C; Division of Epigenetics, German Cancer Research Center (DKFZ), Heidelberg, Germany and Faculty of Biosciences, University of Heidelberg, Heidelberg, Germany.
Tuorto F; Division of Biochemistry, Mannheim Institute for Innate Immunoscience (MI3), Medical Faculty Mannheim, Heidelberg University, Mannheim, and Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg, Germany. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2298, pp. 217-230.
Typ publikacji :
Journal Article
MeSH Terms :
Blotting, Northern/*methods
Nucleoside Q/*genetics
RNA Processing, Post-Transcriptional/*genetics
RNA, Transfer/*genetics
Animals ; Boronic Acids/metabolism ; Humans
Czasopismo naukowe
Tytuł :
Cloning and Detection of Genomic Retrozymes and Their circRNA Intermediates.
Autorzy :
Cervera A; Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València (CSIS-UPV), Valencia, Spain.
de la Peña M; Instituto de Biología Molecular y Celular de Plantas (IBMCP), Consejo Superior de Investigaciones Científicas-Universitat Politècnica de València (CSIS-UPV), Valencia, Spain. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2167, pp. 27-44.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Blotting, Northern/*methods
Cloning, Molecular/*methods
RNA, Catalytic/*genetics
RNA, Catalytic/*isolation & purification
RNA, Circular/*genetics
Retroelements/*genetics
Animals ; Genome ; Nucleotide Motifs ; Plants/enzymology ; Plants/genetics ; Plants/metabolism ; RNA, Catalytic/metabolism ; RNA, Circular/metabolism
Czasopismo naukowe
Tytuł :
tRNA-Derived Fragments in Alzheimer's Disease: Implications for New Disease Biomarkers and Neuropathological Mechanisms.
Autorzy :
Wu W; Department of Pediatrics, The University of Texas Medical Branch, Galveston, TX, USA.
Lee I; miRcore, Ann Arbor, MI, USA.
Spratt H; Department of Preventive Medicine and Population Health, The University of Texas Medical Branch, Galveston, TX, USA.; The Institute of Translational Sciences, The University of Texas Medical Branch, Galveston, TX, USA.
Fang X; Department of Neurology and Mitchell Center for Neurodegenerative Diseases, The University of Texas Medical Branch, Galveston, TX, USA.
Bao X; Department of Pediatrics, The University of Texas Medical Branch, Galveston, TX, USA.; The Institute of Translational Sciences, The University of Texas Medical Branch, Galveston, TX, USA.; Sealy Center for Molecular Medicine, and The University of Texas Medical Branch, Galveston, TX, USA.; The Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, TX, USA.
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Źródło :
Journal of Alzheimer's disease : JAD [J Alzheimers Dis] 2021; Vol. 79 (2), pp. 793-806.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Alzheimer Disease/*diagnosis
Hippocampus/*metabolism
RNA, Transfer/*metabolism
Aged ; Alzheimer Disease/etiology ; Alzheimer Disease/pathology ; Biomarkers ; Blotting, Northern ; Case-Control Studies ; Humans ; Polymerase Chain Reaction ; RNA, Small Untranslated
Czasopismo naukowe
Tytuł :
Design and Evaluation of AgoshRNAs with 3'-Terminal HDV Ribozymes to Enhance the Silencing Activity.
Autorzy :
Berkhout B; Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. .
Herrera-Carrillo E; Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2167, pp. 225-252.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Silencing*
Argonaute Proteins/*genetics
HIV Infections/*genetics
HIV-1/*genetics
Hepatitis Delta Virus/*genetics
RNA, Catalytic/*genetics
RNA, Guide/*genetics
RNA, Small Interfering/*genetics
Argonaute Proteins/metabolism ; Blotting, Northern ; Cloning, Molecular/methods ; Enzyme Assays/methods ; Genetic Vectors ; HEK293 Cells ; HIV-1/metabolism ; Humans ; Inverted Repeat Sequences/genetics ; Luciferases/metabolism ; RNA Interference ; RNA, Catalytic/metabolism ; RNA, Guide/metabolism ; RNA, Small Interfering/metabolism ; T-Lymphocytes/virology ; Transfection/methods
Czasopismo naukowe
Tytuł :
Use of a Lariat Capping Ribozyme to Study Cap Function In Vivo.
Autorzy :
Pietschmann M; Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.
Tempel G; Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.
Halladjian M; Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.
Krogh N; Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark.
Nielsen H; Department of Cellular and Molecular Medicine, University of Copenhagen, Copenhagen, Denmark. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2167, pp. 271-285.
Typ publikacji :
Journal Article
MeSH Terms :
Dinucleoside Phosphates/*metabolism
RNA, Catalytic/*genetics
RNA, Catalytic/*metabolism
RNA, Messenger/*metabolism
Yeasts/*metabolism
Blotting, Northern ; Dinucleoside Phosphates/genetics ; Electrophoresis, Polyacrylamide Gel ; Exonucleases/metabolism ; Flow Cytometry ; Internal Ribosome Entry Sites/genetics ; Models, Molecular ; Nucleic Acid Conformation ; RNA Caps/metabolism ; RNA, Messenger/chemistry ; Yeasts/genetics
Czasopismo naukowe
Tytuł :
Methods for the Study of Long Noncoding RNA in Cancer Cell Signaling.
Autorzy :
Feng Y; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Jiang J; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Hu Z; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Yuan J; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Zhang T; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Pan Y; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Xu M; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Li C; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Zhang Y; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Zhang L; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.; Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Hu X; Center for Reproduction and Women's Health, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. .; Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2174, pp. 89-118.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Molecular Biology/*methods
Neoplasms/*genetics
Neoplasms/*metabolism
RNA, Long Noncoding/*genetics
Biomarkers, Tumor/genetics ; Blotting, Northern/methods ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Genes, Tumor Suppressor ; Genome, Human ; Humans ; Immunoprecipitation/methods ; In Situ Hybridization/methods ; Neoplasms/pathology ; Oncogenes ; RNA, Long Noncoding/metabolism ; Signal Transduction
Czasopismo naukowe
Tytuł :
Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation.
Autorzy :
Vayssières M; Laboratoire CiTCoM 'Cibles Thérapeutique et Conception des Médicaments', CNRS UMR 8038, Faculté de Pharmacie, Université de Paris, Paris, France.
Bourgeois G; Laboratoire de Biochimie, CNRS UMR 7654, Ecole Polytechnique, Palaiseau, France.
Chardon F; ZMBE Zentrum für Molekularbuiologie der Entzündung, Münster, Germany.
Tillault AS; Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, Lethbridge, AB, Canada.
Blaud M; Laboratoire CiTCoM 'Cibles Thérapeutique et Conception des Médicaments', CNRS UMR 8038, Faculté de Pharmacie, Université de Paris, Paris, France. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2300, pp. 65-72.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
MicroRNAs/*analysis
MicroRNAs/*chemistry
Oligonucleotide Probes/*metabolism
Blotting, Northern ; DNA Ligases/metabolism ; Nucleic Acid Hybridization ; Oligonucleotide Probes/chemistry ; Staining and Labeling
Czasopismo naukowe
Tytuł :
Cloning and Detection of Aptamer-Ribozyme Conjugations.
Autorzy :
Goguen RP; Lady Davis Institute for Medical Research, Montréal, QC, Canada.; Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada.
Gatignol A; Lady Davis Institute for Medical Research, Montréal, QC, Canada. .; Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada. .; Department of Medicine, Division of Experimental Medicine, McGill University, Montréal, QC, Canada. .
Scarborough RJ; Lady Davis Institute for Medical Research, Montréal, QC, Canada. .; Department of Microbiology and Immunology, McGill University, Montréal, QC, Canada. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2167, pp. 253-267.
Typ publikacji :
Journal Article
MeSH Terms :
Aptamers, Nucleotide/*genetics
Aptamers, Nucleotide/*isolation & purification
Cloning, Molecular/*methods
Polymerase Chain Reaction/*methods
RNA, Catalytic/*genetics
RNA, Catalytic/*isolation & purification
Aptamers, Nucleotide/chemistry ; Blotting, Northern ; Electrophoresis, Polyacrylamide Gel ; Genetic Vectors ; HEK293 Cells ; Humans ; RNA, Catalytic/chemistry ; RNA, Small Untranslated/isolation & purification
Czasopismo naukowe
Tytuł :
Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting.
Autorzy :
Krüßel L; Molecular Plant Genetics, Universität Hamburg, Institute of Plant Science and Microbiology, Hamburg, Germany.
Ostendorp S; Molecular Plant Genetics, Universität Hamburg, Institute of Plant Science and Microbiology, Hamburg, Germany.
Ostendorp A; Molecular Plant Genetics, Universität Hamburg, Institute of Plant Science and Microbiology, Hamburg, Germany.
Kehr J; Molecular Plant Genetics, Universität Hamburg, Institute of Plant Science and Microbiology, Hamburg, Germany. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2170, pp. 45-51.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Blotting, Northern/*methods
RNA/*chemistry
RNA-Binding Proteins/*chemistry
RNA-Binding Proteins/*metabolism
Ribonucleoproteins/*chemistry
Ribonucleoproteins/*metabolism
Nucleic Acids/chemistry ; Nucleic Acids/metabolism
Czasopismo naukowe
Tytuł :
Northern Blot Detection of Tiny RNAs.
Autorzy :
Wiegard JC; Institute of Pharmaceutical Chemistry, Philipps-University Marburg, Marburg, Germany.
Schlüter MAC; Institute of Pharmaceutical Chemistry, Philipps-University Marburg, Marburg, Germany.
Burenina OY; Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, Russia.; Chemistry Department and A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
Kubareva EA; Chemistry Department and A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
Klug G; Institute of Microbiology and Molecular Biology, Justus-Liebig-University-Gießen, Gießen, Germany.
Grünweller A; Institute of Pharmaceutical Chemistry, Philipps-University Marburg, Marburg, Germany.
Hartmann RK; Institute of Pharmaceutical Chemistry, Philipps-University Marburg, Marburg, Germany. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2300, pp. 41-58.
Typ publikacji :
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cross-Linking Reagents/*chemistry
DNA Probes/*metabolism
Ethyldimethylaminopropyl Carbodiimide/*chemistry
RNA/*analysis
Blotting, Northern ; DNA Probes/chemistry ; Denaturing Gradient Gel Electrophoresis ; Digoxigenin/chemistry ; Native Polyacrylamide Gel Electrophoresis ; RNA/chemistry
Czasopismo naukowe
Tytuł :
Methods for detection and study of virus-derived small RNAs produced from the intramolecular base-pairing region of the picornavirus genome.
Autorzy :
Lee KM; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Gong YN; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan.
Shih SR; Research Center for Emerging Viral Infections, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, Taiwan; Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Research Center for Chinese Herbal Medicine, Research Center for Food and Cosmetic Safety, and Graduate Institute of Health Industry Technology, College of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan. Electronic address: .
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Źródło :
Methods (San Diego, Calif.) [Methods] 2020 Nov 01; Vol. 183, pp. 4-12. Date of Electronic Publication: 2019 Sep 05.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Genome, Viral*
Blotting, Northern/*methods
Enterovirus A, Human/*genetics
RNA, Viral/*analysis
Animals ; Cell Line, Tumor ; DEAD-box RNA Helicases ; Electrophoresis, Polyacrylamide Gel/methods ; Electrophoretic Mobility Shift Assay/methods ; Gene Expression Regulation, Viral ; Humans ; Internal Ribosome Entry Sites/genetics ; Mice ; RNA Interference ; RNA, Small Interfering ; RNA, Viral/metabolism ; Ribonuclease III ; Sequence Analysis, RNA/methods ; Virus Replication/genetics
Czasopismo naukowe

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