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Tytuł :
Differential Efficacy of Novel Antiviral Substances in 3D and Monolayer Cell Culture.
Autorzy :
Koban R; Highly Pathogenic Viruses, Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Seestr. 10, 13353 Berlin, Germany.
Neumann M; Highly Pathogenic Viruses, Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Seestr. 10, 13353 Berlin, Germany.
Nelson PP; Highly Pathogenic Viruses, Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Seestr. 10, 13353 Berlin, Germany.
Ellerbrok H; Highly Pathogenic Viruses, Centre for Biological Threats and Special Pathogens, Robert Koch Institute, Seestr. 10, 13353 Berlin, Germany.; Public Health Laboratory Support, Centre for International Health Protection, Nordufer 20, 13353 Berlin, Germany.
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Źródło :
Viruses [Viruses] 2020 Nov 12; Vol. 12 (11). Date of Electronic Publication: 2020 Nov 12.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Culture Techniques*
Antiviral Agents/*pharmacology
Drug Discovery/*methods
Cell Line ; Cell Survival/drug effects ; Drug Synergism ; ErbB Receptors/antagonists & inhibitors ; ErbB Receptors/metabolism ; High-Throughput Screening Assays/methods ; Poxviridae/drug effects ; Poxviridae/physiology ; Primary Cell Culture ; Protein Kinase Inhibitors/pharmacology ; Spheroids, Cellular ; Virus Replication
Czasopismo naukowe
Tytuł :
Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells.
Autorzy :
Dobrica MO; Institute of Biochemistry of the Romanian Academy, Bucharest, Romania.
Lazar C; Institute of Biochemistry of the Romanian Academy, Bucharest, Romania.
Branza-Nichita N; Institute of Biochemistry of the Romanian Academy, Bucharest, Romania. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2183, pp. 83-94.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Batch Cell Culture Techniques*
Recombinant Fusion Proteins*
Hepatitis B Surface Antigens/*biosynthesis
Animals ; Cell Culture Techniques ; Gene Expression ; HEK293 Cells ; Hepatitis B Surface Antigens/genetics ; Hepatitis B Surface Antigens/immunology ; Hepatitis B Surface Antigens/isolation & purification ; Hepatitis B Vaccines/biosynthesis ; Hepatitis B Vaccines/immunology ; Humans ; Transfection
Czasopismo naukowe
Tytuł :
Production of Zika Virus Virus-Like Particles.
Autorzy :
Kuadkitkan A; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Ramphan S; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Worawichawong S; Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Sornjai W; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Wikan N; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Smith DR; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2183, pp. 183-203.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Batch Cell Culture Techniques*
Vaccines, Virus-Like Particle/*biosynthesis
Vaccines, Virus-Like Particle/*immunology
Zika Virus/*immunology
Cell Culture Techniques ; Cloning, Molecular ; Gene Expression ; Genetic Engineering ; Genetic Vectors/genetics ; HEK293 Cells ; Humans ; Plasmids/genetics ; Vaccines, Virus-Like Particle/isolation & purification ; Vaccines, Virus-Like Particle/ultrastructure
Czasopismo naukowe
Tytuł :
Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System.
Autorzy :
Harnischfeger J; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Käßer L; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Zitzmann J; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Salzig D; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Czermak P; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany. .; Faculty of Biology and Chemistry, Justus-Liebig-University Giessen, Giessen, Germany. .; Division Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, Germany. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2183, pp. 95-118.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Batch Cell Culture Techniques*
Bioreactors*
Recombinant Proteins*
Antigens/*biosynthesis
Antigens/*genetics
Baculoviridae/*genetics
Genetic Vectors/*genetics
Animals ; Antigens/isolation & purification ; Cell Culture Techniques ; Cloning, Molecular ; Gene Expression ; Genetic Engineering ; Sf9 Cells ; Transfection ; Workflow
Czasopismo naukowe
Tytuł :
Generation of Murine Primary Colon Epithelial Monolayers from Intestinal Crypts.
Autorzy :
Muraleedharan CK; Department of Pathology, School of Medicine, University of Michigan.
Mierzwiak J; Department of Pathology, School of Medicine, University of Michigan.
Feier D; Department of Pathology, School of Medicine, University of Michigan.
Nusrat A; Department of Pathology, School of Medicine, University of Michigan.
Quiros M; Department of Pathology, School of Medicine, University of Michigan; .
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Źródło :
Journal of visualized experiments : JoVE [J Vis Exp] 2021 Feb 06 (168). Date of Electronic Publication: 2021 Feb 06.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media
MeSH Terms :
Cell Differentiation*
Colon/*cytology
Epithelial Cells/*cytology
Intestinal Mucosa/*cytology
Primary Cell Culture/*methods
Animals ; Cell Culture Techniques/methods ; Cells, Cultured ; Mice ; Mice, Inbred C57BL
Czasopismo naukowe
Tytuł :
Magnetic separation of peripheral nerve-resident cells underscores key molecular features of human Schwann cells and fibroblasts: an immunochemical and transcriptomics approach.
Autorzy :
Peng K; Stark Neurosciences Research Institute and Department of Neurological Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.; Division of Spine Surgery, Department of Orthopedics, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Sant D; Department of Human Genetics, University of Miami Miller School of Medicine, Miami, FL, USA.; University of Utah, Salt Lake City, UT, USA.
Andersen N; The Miami Project To Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL, USA.; Instituto de Investigaciones Bioquímicas de Bahía Blanca (CONICET), Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Bahía Blanca, Argentina.
Silvera R; The Miami Project To Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL, USA.; Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
Camarena V; Department of Human Genetics, University of Miami Miller School of Medicine, Miami, FL, USA.
Piñero G; The Miami Project To Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL, USA.; Facultad de Farmacia Y Bioquímica, Departamento de Química Biológica, and CONICET, Instituto de Química Y Fisicoquímica Biológicas (IQUIFIB), Universidad de Buenos Aires, Buenos Aires, Argentina.
Graham R; Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, FL, USA.
Khan A; The Miami Project To Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL, USA.; Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, Miami, FL, USA.
Xu XM; Stark Neurosciences Research Institute and Department of Neurological Surgery, Indiana University School of Medicine, Indianapolis, IN, USA.
Wang G; Department of Human Genetics, University of Miami Miller School of Medicine, Miami, FL, USA.
Monje PV; Stark Neurosciences Research Institute and Department of Neurological Surgery, Indiana University School of Medicine, Indianapolis, IN, USA. .; The Miami Project To Cure Paralysis, University of Miami Miller School of Medicine, Miami, FL, USA. .; Department of Neurological Surgery, University of Miami Miller School of Medicine, Miami, FL, USA. .
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Źródło :
Scientific reports [Sci Rep] 2020 Oct 28; Vol. 10 (1), pp. 18433. Date of Electronic Publication: 2020 Oct 28.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Validation Study
MeSH Terms :
Cell Culture Techniques*
Cell Separation/*methods
Fibroblasts/*cytology
Peripheral Nerves/*cytology
Schwann Cells/*cytology
Adolescent ; Adult ; Aged ; Cell Line ; Child ; Cluster Analysis ; Female ; Gene Expression Profiling ; Humans ; Male ; Middle Aged ; Nerve Regeneration ; Primary Cell Culture ; Workflow ; Young Adult
Czasopismo naukowe
Tytuł :
Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes.
Autorzy :
Stefanska A; BHF Centre for Cardiovascular Science, Queen's Medical Research Center, University of Edinburgh, Edinburgh, UK.; Comprehensive Pneumology Center, Institute of Lung Biology and Disease, Helmholtz Zentrum München, German Center for Lung Research (DZL), Munich, Germany.
Briski A; BHF Centre for Cardiovascular Science, Queen's Medical Research Center, University of Edinburgh, Edinburgh, UK.; MRC for Regenerative Medicine, University of Edinburgh, Edinburgh, UK.
Khan NS; BHF Centre for Cardiovascular Science, Queen's Medical Research Center, University of Edinburgh, Edinburgh, UK.; MRC for Regenerative Medicine, University of Edinburgh, Edinburgh, UK.
Kenyon C; BHF Centre for Cardiovascular Science, Queen's Medical Research Center, University of Edinburgh, Edinburgh, UK.
Péault BM; Orthopedic Hospital Research Center and David Geffen School of Medicine, University of California, Los Angeles, CA, USA.; BHF Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK.
Mullins JJ; BHF Centre for Cardiovascular Science, Queen's Medical Research Center, University of Edinburgh, Edinburgh, UK. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2235, pp. 169-180.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Pericytes/*metabolism
Primary Cell Culture/*methods
Renin-Angiotensin System/*physiology
Angiotensins/metabolism ; Animals ; Cell Culture Techniques/methods ; Cell Differentiation/physiology ; Humans ; Kidney/metabolism ; Renin/metabolism ; Renin-Angiotensin System/drug effects
Czasopismo naukowe
Tytuł :
Analysis of Cytomegalovirus Glycoprotein and Cellular Receptor Interactions.
Autorzy :
Mahmud J; Department of Microbiology & Immunology, SUNY Upstate Medical University, Syracuse, NY, USA.
Chan GC; Department of Microbiology & Immunology, SUNY Upstate Medical University, Syracuse, NY, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2244, pp. 199-211.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Culture Techniques/*methods
Cytomegalovirus/*metabolism
Membrane Glycoproteins/*metabolism
Animals ; Cell Line/virology ; Cytomegalovirus/genetics ; Fibroblasts/metabolism ; Humans ; Primary Cell Culture/methods ; Signal Transduction/genetics ; Signal Transduction/physiology ; Viral Envelope Proteins/metabolism ; Virus Internalization
Czasopismo naukowe
Tytuł :
Methods for Studying the Function of Cytomegalovirus GPCRs.
Autorzy :
O'Connor CM; Genomic Medicine, Lerner Research Institute, Cleveland Clinic, Cleveland, OH, USA. .
Miller WE; Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2244, pp. 159-197.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques/*methods
Cytomegalovirus/*genetics
Receptors, G-Protein-Coupled/*genetics
Animals ; Cell Line/virology ; Cytomegalovirus/metabolism ; Humans ; Primary Cell Culture/methods ; Signal Transduction/genetics ; Signal Transduction/physiology
Czasopismo naukowe
Tytuł :
Establishment and Characterization of Cell Lines from Primary Culture of Hemangioblastoma Stromal Cells.
Autorzy :
Liu W; Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
Zhang S; Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
Hui X; Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
You C; Department of Neurosurgery, West China Hospital of Sichuan University, Chengdu, Sichuan, China.
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Źródło :
Neurology India [Neurol India] 2020 Mar-Apr; Vol. 68 (2), pp. 383-388.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Line, Tumor*
Primary Cell Culture*
Brain Neoplasms/*pathology
Hemangioblastoma/*pathology
Adult ; Brain Neoplasms/ultrastructure ; Cell Culture Techniques ; Cell Proliferation ; Cell Survival ; Cryopreservation ; Female ; Hemangioblastoma/ultrastructure ; Humans ; Immunohistochemistry ; Male ; Microscopy, Electron, Transmission ; Middle Aged ; Young Adult
Czasopismo naukowe
Tytuł :
Isolation and characterization of the first Slovenian human triple-negative breast cancer cell line.
Autorzy :
Skok K; Faculty of Medicine, Institute of Biomedical Sciences and Department of Pharmacology, University of Maribor, Maribor, Slovenia.; Department of pathology, General Hospital Graz II, Location West, Graz, Austria.
Gradišnik L; Faculty of Medicine, Institute of Biomedical Sciences and Department of Pharmacology, University of Maribor, Maribor, Slovenia.
Čelešnik H; Faculty of Medicine, Center of Human Molecular Genetics and Pharmacogenomics, University of Maribor, Maribor, Slovenia.; Faculty of Chemistry & Chemical Engineering, University of Maribor, Maribor, Slovenia.
Potočnik U; Faculty of Medicine, Center of Human Molecular Genetics and Pharmacogenomics, University of Maribor, Maribor, Slovenia.; Faculty of Chemistry & Chemical Engineering, University of Maribor, Maribor, Slovenia.
Kavalar R; Department of Pathology, University Medical Centre Maribor, Maribor, Slovenia.
Takač I; Faculty of Medicine, Department of Gynaecology and Obstetrics, University of Maribor, Maribor, Slovenia.; Department of Gynaecology and Perinatology, University Medical Centre Maribor, Maribor, Slovenia.
Maver U; Faculty of Medicine, Institute of Biomedical Sciences and Department of Pharmacology, University of Maribor, Maribor, Slovenia.
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Źródło :
The breast journal [Breast J] 2020 Feb; Vol. 26 (2), pp. 328-330. Date of Electronic Publication: 2019 Nov 20.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Cell Line, Tumor*
Cell Separation*
Triple Negative Breast Neoplasms*
Female ; Humans ; Middle Aged ; Primary Cell Culture ; Slovenia
Czasopismo naukowe
Tytuł :
Isolation and enrichment of melanocytes from human corneal limbus using CD117 (c-Kit) as selection marker.
Autorzy :
Polisetti N; Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Killianstrasse 5, 79106, Freiburg, Germany. .
Schlötzer-Schrehardt U; Department of Ophthalmology, University Hospital Erlangen, Friedrich-Alexander-University of Erlangen-Nürnberg, Schwabachanlage 6, 91054, Erlangen, Germany.
Reinhard T; Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Killianstrasse 5, 79106, Freiburg, Germany.
Schlunck G; Eye Center, Medical Center, Faculty of Medicine, University of Freiburg, Killianstrasse 5, 79106, Freiburg, Germany. .
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Źródło :
Scientific reports [Sci Rep] 2020 Oct 16; Vol. 10 (1), pp. 17588. Date of Electronic Publication: 2020 Oct 16.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Culture Techniques/*methods
Limbus Corneae/*cytology
Melanocytes/*cytology
Biomarkers/metabolism ; Epithelium, Corneal/cytology ; Humans ; Limbus Corneae/metabolism ; Melanocytes/metabolism ; Primary Cell Culture/methods ; Proto-Oncogene Proteins c-kit/immunology ; Proto-Oncogene Proteins c-kit/metabolism ; Stem Cell Niche/physiology ; Stem Cells/cytology ; Tissue Engineering/methods
Czasopismo naukowe
Tytuł :
Isolation and Culture of Human Skin Mast Cells.
Autorzy :
Siiskonen H; Department of Dermatology and Allergy, Allergie-Centrum-Charité, Charité-Universitätsmedizin Berlin, Berlin, Germany. .; Department of Dermatology, Kuopio University Hospital and University of Eastern Finland, Kuopio, Finland. .
Scheffel J; Department of Dermatology and Allergy, Allergie-Centrum-Charité, Charité-Universitätsmedizin Berlin, Berlin, Germany.
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2154, pp. 33-43.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Cell Separation/*methods
Mast Cells/*cytology
Mast Cells/*metabolism
Skin/*cytology
Flow Cytometry ; Humans ; Immunomagnetic Separation/methods ; Primary Cell Culture
Czasopismo naukowe
Tytuł :
A systematic review on thyroid organoid models: time-trend and its achievements.
Autorzy :
Samimi H; Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Atlasi R; Evidence Based Practice Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Parichehreh-Dizaji S; Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
Khazaei S; Department of Cell and Molecular Biology, Faculty of Biological Science and Technology, Isfahan University, Isfahan, Iran.; Department of Hematology and Cell Therapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Akhavan Rahnama M; Department of Hematology and Cell Therapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Seifirad S; Department of Internal Medicine, Hackensack Meridian Health Mountainside Medical Center, Montclair, New Jersey.
Haghpanah V; Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.; Personalized Medicine Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
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Źródło :
American journal of physiology. Endocrinology and metabolism [Am J Physiol Endocrinol Metab] 2021 Mar 01; Vol. 320 (3), pp. E581-E590. Date of Electronic Publication: 2021 Jan 11.
Typ publikacji :
Historical Article; Journal Article; Systematic Review
MeSH Terms :
Cell Culture Techniques/*methods
Cell Culture Techniques/*trends
Organoids/*cytology
Thyroid Gland/*cytology
Cell Culture Techniques/history ; History, 20th Century ; History, 21st Century ; Humans ; Models, Biological ; Regenerative Medicine/history ; Regenerative Medicine/methods ; Regenerative Medicine/trends
Czasopismo naukowe
Tytuł :
Potential Effects of Nonadherent on Adherent Human Umbilical Venous Endothelial Cells in Cell Culture.
Autorzy :
Schulz C; Fraunhofer Project Group PZ-Syn of the Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses (IZI-BB), 14476 Potsdam-Golm, Germany, Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, 01968 Brandenburg, Germany.; Institute of Active Polymers and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513 Teltow, Germany.
Krüger-Genge A; Institute of Active Polymers and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513 Teltow, Germany.; Department of Anesthesia, Pain Management and Perioperative Medicine, Faculty of Medicine, Dalhousie University, Halifax, NS 6299, Canada.
Lendlein A; Institute of Active Polymers and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513 Teltow, Germany.; Institute of Chemistry, University of Potsdam, Karl-Liebknecht-Straße 24-25, 14469 Potsdam, Germany.
Küpper JH; Institute of Biotechnology, Molecular Cell Biology, Brandenburg University of Technology, 01968 Senftenberg, Germany.
Jung F; Institute of Active Polymers and Berlin-Brandenburg Center for Regenerative Therapies, Helmholtz-Zentrum Geesthacht, 14513 Teltow, Germany.; Institute of Biotechnology, Molecular Cell Biology, Brandenburg University of Technology, 01968 Senftenberg, Germany.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 Feb 02; Vol. 22 (3). Date of Electronic Publication: 2021 Feb 02.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Cell Culture Techniques*/instrumentation
Cell Culture Techniques*/methods
Cell Adhesion/*physiology
Human Umbilical Vein Endothelial Cells/*cytology
Apoptosis ; Cell Death ; Cell Division ; Culture Media, Conditioned/chemistry ; Cytokines/analysis ; Epoprostenol/analysis ; Human Umbilical Vein Endothelial Cells/drug effects ; Humans ; Inflammation Mediators/analysis ; Intercellular Signaling Peptides and Proteins/analysis ; L-Lactate Dehydrogenase/analysis ; Polystyrenes ; Recombinant Proteins/pharmacology ; Surface Properties ; Thromboxane A2/analysis ; Tumor Necrosis Factor-alpha/pharmacology
Czasopismo naukowe
Tytuł :
Large-scale in vitro production of red blood cells from human peripheral blood mononuclear cells.
Autorzy :
Heshusius S; Sanquin Research, Department of Hematopoiesis and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.; ​Department of Cell Biology, Erasmus Medical Center, Rotterdam, The Netherlands.
Heideveld E; Sanquin Research, Department of Hematopoiesis and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
Burger P; Sanquin Research, Department of Hematopoiesis and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.; Laboratory for Cell Therapy, Sanquin Research, Amsterdam, The Netherlands; and.
Thiel-Valkhof M; Laboratory for Cell Therapy, Sanquin Research, Amsterdam, The Netherlands; and.
Sellink E; Laboratory for Cell Therapy, Sanquin Research, Amsterdam, The Netherlands; and.
Varga E; Sanquin Research, Department of Hematopoiesis and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
Ovchynnikova E; Sanquin Research, Department of Hematopoiesis and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
Visser A; Sanquin Research, Department of Hematopoiesis and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
Martens JHA; Department of Molecular Biology, Faculty of Science, Nijmegen Centre for Molecular Life Sciences, Radboud University, Nijmegen, The Netherlands.
von Lindern M; Sanquin Research, Department of Hematopoiesis and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
van den Akker E; Sanquin Research, Department of Hematopoiesis and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands.
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Źródło :
Blood advances [Blood Adv] 2019 Nov 12; Vol. 3 (21), pp. 3337-3350.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Batch Cell Culture Techniques*/standards
Cell Differentiation*/genetics
Erythropoiesis*/genetics
Erythroblasts/*cytology
Erythrocytes/*cytology
Leukocytes, Mononuclear/*cytology
Biomarkers ; Bioreactors ; Cell Proliferation ; Erythroblasts/metabolism ; Erythrocytes/metabolism ; Gene Expression Profiling ; Humans ; Immunophenotyping ; Leukocytes, Mononuclear/metabolism ; Primary Cell Culture ; Reticulocytes/metabolism ; Transcriptome
Czasopismo naukowe
Tytuł :
Short-term cultured autologous peripheral blood mononuclear cells as a potential immunogen to activate Tax-specific CTL response in adult T-cell leukemia patients.
Autorzy :
Ishizawa M; Deparment of Immunotherapeutics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Ganbaatar U; Deparment of Immunotherapeutics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Hasegawa A; Deparment of Immunotherapeutics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Takatsuka N; Deparment of Immunotherapeutics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Kondo N; Deparment of Immunotherapeutics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Yoneda T; Deparment of Immunotherapeutics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Katagiri K; Deparment of Immunotherapeutics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Masuda T; Deparment of Immunotherapeutics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
Utsunomiya A; Department of Hematology, Imamura General Hospital, Kagoshima, Japan.
Kannagi M; Deparment of Immunotherapeutics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.; Department of Molecular Virology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.; Department of Microbiology, Kansai Medical University, Osaka, Japan.
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Źródło :
Cancer science [Cancer Sci] 2021 Mar; Vol. 112 (3), pp. 1161-1172. Date of Electronic Publication: 2021 Feb 05.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Cancer Vaccines/*administration & dosage
HTLV-I Infections/*therapy
Immunotherapy/*methods
Leukemia-Lymphoma, Adult T-Cell/*therapy
Leukocytes, Mononuclear/*transplantation
Antigens, Viral/immunology ; Cancer Vaccines/immunology ; Cell Line, Tumor ; Coculture Techniques ; Cross-Priming/immunology ; Gene Products, tax/immunology ; HLA-A2 Antigen/immunology ; HLA-A2 Antigen/metabolism ; HTLV-I Infections/blood ; HTLV-I Infections/immunology ; Histone Deacetylase Inhibitors/pharmacology ; Human T-lymphotropic virus 1/immunology ; Humans ; Leukemia-Lymphoma, Adult T-Cell/blood ; Leukemia-Lymphoma, Adult T-Cell/immunology ; Leukocytes, Mononuclear/drug effects ; Leukocytes, Mononuclear/immunology ; Mitomycin/pharmacology ; T-Lymphocytes, Cytotoxic/immunology ; Time Factors ; Transplantation, Autologous
Czasopismo naukowe
Tytuł :
In Vitro Development of Human iPSC-Derived Functional Neuronal Networks on Laser-Fabricated 3D Scaffolds.
Autorzy :
Koroleva A; Institute of Quantum Optics, Leibniz University Hannover, 30167 Hannover, Germany.; Institute for Regenerative Medicine, Sechenov University, 119991 Moscow, Russia.; Laser Zentrum Hannover e.V., 30419 Hannover, Germany.
Deiwick A; Institute of Quantum Optics, Leibniz University Hannover, 30167 Hannover, Germany.
El-Tamer A; Laser Zentrum Hannover e.V., 30419 Hannover, Germany.
Koch L; Institute of Quantum Optics, Leibniz University Hannover, 30167 Hannover, Germany.
Shi Y; Axol Bioscience Ltd., CB10 1XL Cambridge, UK.
Estévez-Priego E; Departament de Física de la Matèria Condensada, Universitat de Barcelona, 08028 Barcelona, Spain.; Universitat de Barcelona Institute of Complex Systems (UBICS), 08028 Barcelona, Spain.
Ludl AA; Departament de Física de la Matèria Condensada, Universitat de Barcelona, 08028 Barcelona, Spain.; Universitat de Barcelona Institute of Complex Systems (UBICS), 08028 Barcelona, Spain.; Computational Biology Unit, Department of Informatics, University of Bergen, 5020 Bergen, Norway.
Soriano J; Departament de Física de la Matèria Condensada, Universitat de Barcelona, 08028 Barcelona, Spain.; Universitat de Barcelona Institute of Complex Systems (UBICS), 08028 Barcelona, Spain.
Guseva D; Cellular Neurophysiology, Hannover Medical School, 30625 Hannover, Germany.; Department of Nutritional Medicine, University of Hohenheim, 70599 Stuttgart, Germany.
Ponimaskin E; Cellular Neurophysiology, Hannover Medical School, 30625 Hannover, Germany.
Chichkov B; Institute of Quantum Optics, Leibniz University Hannover, 30167 Hannover, Germany.
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Źródło :
ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2021 Feb 24; Vol. 13 (7), pp. 7839-7853. Date of Electronic Publication: 2021 Feb 09.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Lasers*
Neural Networks, Computer*
Induced Pluripotent Stem Cells/*cytology
Cells, Cultured ; Humans
Czasopismo naukowe
Tytuł :
Cell quality evaluation with gene expression analysis of spheroids (3D) and adherent (2D) adipose stem cells.
Autorzy :
Di Stefano AB; BIOPLAST-Laboratory of BIOlogy and Regenerative Medicine-PLASTic Surgery, Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy. Electronic address: .
Grisafi F; BIOPLAST-Laboratory of BIOlogy and Regenerative Medicine-PLASTic Surgery, Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy.
Perez-Alea M; Advanced BioDesign, Parc Technologique de Lyon, Woodstock - Bâtiment Cèdre 1, Saint Priest, France.
Castiglia M; Medical Oncology, Department of Surgical, Oncological and Oral Sciences, University of Palermo, Palermo, Italy.
Di Simone M; Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), University of Palermo, Palermo, Italy; Department of Biomedicine, Neurosciences and Advanced Diagnosis, University of Palermo, Palermo, Italy.
Meraviglia S; Central Laboratory of Advanced Diagnosis and Biomedical Research (CLADIBIOR), University of Palermo, Palermo, Italy; Department of Biomedicine, Neurosciences and Advanced Diagnosis, University of Palermo, Palermo, Italy.
Cordova A; BIOPLAST-Laboratory of BIOlogy and Regenerative Medicine-PLASTic Surgery, Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy; Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy; Plastic and Reconstructive Surgery, Department of Oncology, Azienda Ospedaliera Universitaria Policlinico 'Paolo Giaccone', 90127 Palermo, Italy.
Moschella F; BIOPLAST-Laboratory of BIOlogy and Regenerative Medicine-PLASTic Surgery, Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy.
Toia F; BIOPLAST-Laboratory of BIOlogy and Regenerative Medicine-PLASTic Surgery, Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy; Plastic and Reconstructive Surgery, Department of Surgical, Oncological and Oral Sciences, University of Palermo, 90127 Palermo, Italy; Plastic and Reconstructive Surgery, Department of Oncology, Azienda Ospedaliera Universitaria Policlinico 'Paolo Giaccone', 90127 Palermo, Italy.
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Źródło :
Gene [Gene] 2021 Feb 05; Vol. 768, pp. 145269. Date of Electronic Publication: 2020 Oct 24.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Stem Cell Transplantation*
Adipocytes/*metabolism
Adipose Tissue/*cytology
Stem Cells/*cytology
Adipocytes/cytology ; Adolescent ; Adult ; Aged ; Aging/genetics ; Cell Adhesion/genetics ; Cell Survival/genetics ; Cell- and Tissue-Based Therapy/methods ; Cells, Cultured ; Cyclin-Dependent Kinase Inhibitor p16/metabolism ; DNA Repair/genetics ; Female ; Humans ; Male ; Middle Aged ; Oxidative Stress/genetics ; RNA-Binding Proteins/metabolism ; Sirtuins/metabolism ; Spheroids, Cellular/cytology ; Telomere Homeostasis/genetics ; Tissue Engineering/methods ; Young Adult
Czasopismo naukowe
Tytuł :
Polymer fraction including exosomes derived from Chinese hamster ovary cells promoted their growth during serum-free repeated batch culture.
Autorzy :
Takagi M; Division of Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan. Electronic address: .
Jimbo S; Division of Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan.
Oda T; Division of Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan.
Goto Y; Division of Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan.
Fujiwara M; Division of Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan.
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Źródło :
Journal of bioscience and bioengineering [J Biosci Bioeng] 2021 Feb; Vol. 131 (2), pp. 183-189. Date of Electronic Publication: 2020 Oct 10.
Typ publikacji :
Journal Article
MeSH Terms :
Batch Cell Culture Techniques*
Biopolymers/*pharmacology
Culture Media, Serum-Free/*chemistry
Exosomes/*chemistry
Animals ; Biopolymers/isolation & purification ; CHO Cells ; Cell Count ; Cell Proliferation/drug effects ; Cricetinae ; Cricetulus ; Perfusion
Czasopismo naukowe

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