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Tytuł :
Isolation and RNA purification of macrophages/microglia from the adult mouse spinal cord.
Autorzy :
Tsatas O; Centre for Research in Neuroscience, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada.
Ghasemlou N; Departments of Biomedical & Molecular Sciences, Queen's University, Kingston, Ontario, Canada; Departments of Anesthesiology & Perioperative Medicine, Queen's University, Kingston, Ontario, Canada; Centre for Neuroscience Studies, Queen's University, Kingston, Ontario, Canada. Electronic address: .
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Źródło :
Journal of immunological methods [J Immunol Methods] 2020 Feb; Vol. 477, pp. 112678. Date of Electronic Publication: 2019 Oct 15.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Separation/*methods
Macrophages/*immunology
Microglia/*immunology
Spinal Cord/*cytology
Animals ; Antibodies, Monoclonal/immunology ; CD11b Antigen/immunology ; Cell Line ; Cells, Cultured ; Centrifugation, Density Gradient/methods ; Female ; Hybridomas ; Mice ; Povidone/chemistry ; RNA/isolation & purification ; Rats ; Silicon Dioxide/chemistry ; Spinal Cord/immunology ; Time Factors
Czasopismo naukowe
Tytuł :
A simple tube adapter to expedite and automate thawing of viably frozen cells.
Autorzy :
Beddall M; ImmunoTechnology Section, Vaccine Research Center, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, United States.
Chattopadhyay PK; ImmunoTechnology Section, Vaccine Research Center, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, United States. Electronic address: .
Kao SF; ImmunoTechnology Section, Vaccine Research Center, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, United States.
Foulds K; ImmunoTechnology Section, Vaccine Research Center, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, United States.
Roederer M; ImmunoTechnology Section, Vaccine Research Center, National Institutes of Health, 40 Convent Drive, Bethesda, MD 20892, United States.
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Źródło :
Journal of immunological methods [J Immunol Methods] 2016 Dec; Vol. 439, pp. 74-78. Date of Electronic Publication: 2016 Sep 01.
Typ publikacji :
Journal Article; Research Support, N.I.H., Intramural
MeSH Terms :
Cryopreservation*
Centrifugation/*instrumentation
T-Lymphocytes/*physiology
Automation, Laboratory ; Cell Survival ; Centrifugation/standards ; Cryoprotective Agents/toxicity ; Culture Media/chemistry ; Equipment Design ; Flow Cytometry ; Humans ; T-Lymphocytes/drug effects ; Time Factors
Czasopismo naukowe
Tytuł :
A simple and effective method for the isolation and culture of human monocytes from small volumes of peripheral blood.
Autorzy :
Meital LT; Centre for Genetics, Ecology & Physiology, School of Health and Sport Sciences, University of the Sunshine Coast, QLD, Australia; VasoActive Research Group, School of Health and Sport Sciences, University of the Sunshine Coast, QLD, Australia.
Coward AS; Centre for Genetics, Ecology & Physiology, School of Health and Sport Sciences, University of the Sunshine Coast, QLD, Australia; VasoActive Research Group, School of Health and Sport Sciences, University of the Sunshine Coast, QLD, Australia.
Windsor MT; VasoActive Research Group, School of Health and Sport Sciences, University of the Sunshine Coast, QLD, Australia.
Bailey TG; VasoActive Research Group, School of Health and Sport Sciences, University of the Sunshine Coast, QLD, Australia; Centre for Research on Exercise, Physical Activity and Health, School of Human Movement and Nutrition Sciences, The University of Queensland, QLD, Australia.
Kuballa A; Centre for Genetics, Ecology & Physiology, School of Health and Sport Sciences, University of the Sunshine Coast, QLD, Australia.
Russell FD; Centre for Genetics, Ecology & Physiology, School of Health and Sport Sciences, University of the Sunshine Coast, QLD, Australia; VasoActive Research Group, School of Health and Sport Sciences, University of the Sunshine Coast, QLD, Australia. Electronic address: .
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Źródło :
Journal of immunological methods [J Immunol Methods] 2019 Sep; Vol. 472, pp. 75-78. Date of Electronic Publication: 2019 Jun 20.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Separation/*methods
Monocytes/*cytology
Aged ; Aged, 80 and over ; Cells, Cultured ; Centrifugation, Density Gradient ; Humans ; Macrophages/cytology ; Male ; Middle Aged
Czasopismo naukowe
Tytuł :
Isolation of tumor endothelial cells from murine cancer.
Autorzy :
Taguchi K; National Hospital Organization, Kure Medical Center/Chugoku Cancer Center, Institute for Clinical Research, 3-1, Aoyamacho, Kure City, Hiroshima 737-0023, Japan; Department of Gastroenterological and Transplant Surgery, Institute of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8551, Japan.
Onoe T; National Hospital Organization, Kure Medical Center/Chugoku Cancer Center, Institute for Clinical Research, 3-1, Aoyamacho, Kure City, Hiroshima 737-0023, Japan; Department of Gastroenterological and Transplant Surgery, Institute of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8551, Japan. Electronic address: .
Yoshida T; National Hospital Organization, Kure Medical Center/Chugoku Cancer Center, Institute for Clinical Research, 3-1, Aoyamacho, Kure City, Hiroshima 737-0023, Japan. Electronic address: .
Yamashita Y; National Hospital Organization, Kure Medical Center/Chugoku Cancer Center, Institute for Clinical Research, 3-1, Aoyamacho, Kure City, Hiroshima 737-0023, Japan. Electronic address: .
Taniyama K; National Hospital Organization, Kure Medical Center/Chugoku Cancer Center, Institute for Clinical Research, 3-1, Aoyamacho, Kure City, Hiroshima 737-0023, Japan. Electronic address: .
Ohdan H; Department of Gastroenterological and Transplant Surgery, Institute of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8551, Japan. Electronic address: .
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Źródło :
Journal of immunological methods [J Immunol Methods] 2019 Jan; Vol. 464, pp. 105-113. Date of Electronic Publication: 2018 Nov 03.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Tumor Microenvironment*
Cell Separation/*methods
Endothelial Cells/*pathology
Melanoma, Experimental/*blood supply
Animals ; CD146 Antigen/immunology ; CD4-Positive T-Lymphocytes/immunology ; Cell Line, Tumor ; Cell Proliferation ; Cell Shape ; Centrifugation, Density Gradient ; Coculture Techniques ; Endothelial Cells/immunology ; Female ; Flow Cytometry ; Immunomagnetic Separation ; Mice, Inbred C57BL ; Phenotype ; Platelet Endothelial Cell Adhesion Molecule-1/immunology
Czasopismo naukowe
Tytuł :
Sample processing approach for detection of ricin in surface samples.
Autorzy :
Kane S; Lawrence Livermore National Laboratory, Livermore, CA, USA.
Shah S; US Environmental Protection Agency, National Homeland Security Research Center, USA. Electronic address: .
Erler AM; Lawrence Livermore National Laboratory, Livermore, CA, USA.
Alfaro T; Lawrence Livermore National Laboratory, Livermore, CA, USA.
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Źródło :
Journal of immunological methods [J Immunol Methods] 2017 Dec; Vol. 451, pp. 54-60. Date of Electronic Publication: 2017 Sep 05.
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Decontamination/*methods
Environmental Monitoring/*methods
Environmental Pollutants/*analysis
Fluoroimmunoassay/*methods
Ricin/*analysis
Centrifugation ; Environmental Pollutants/immunology ; False Negative Reactions ; False Positive Reactions ; Limit of Detection ; Reproducibility of Results ; Ricin/immunology ; Ultrafiltration
Czasopismo naukowe
Tytuł :
Improved recovery of functionally active eosinophils and neutrophils using novel immunomagnetic technology.
Autorzy :
Son K; Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
Mukherjee M; Department of Medicine, McMaster University, Hamilton, Ontario, Canada. Electronic address: .
McIntyre BAS; Miltenyi Biotec, 2303 Lindbergh St, Auburn, CA 95602, USA.
Eguez JC; Universidad de Especialidades Espíritu Santo, School of Medicine, Samborondón, Ecuador.
Radford K; Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
LaVigne N; Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
Ethier C; Pulmonary Research Group, University of Alberta, Edmonton, Alberta, Canada.
Davoine F; Pulmonary Research Group, University of Alberta, Edmonton, Alberta, Canada.
Janssen L; Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
Lacy P; Pulmonary Research Group, University of Alberta, Edmonton, Alberta, Canada.
Nair P; Department of Medicine, McMaster University, Hamilton, Ontario, Canada.
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Źródło :
Journal of immunological methods [J Immunol Methods] 2017 Oct; Vol. 449, pp. 44-55. Date of Electronic Publication: 2017 Jun 21.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Eosinophils/*physiology
Immunomagnetic Separation/*methods
Neutrophils/*physiology
Centrifugation, Density Gradient ; Flow Cytometry ; Humans ; Leukocyte Count
Czasopismo naukowe
Tytuł :
Characterization of human leukocyte-HUVEC adhesion: Effect of cell preparation methods.
Autorzy :
Marino F; Center of Research in Medical Pharmacology, University of Insubria, Varese, Italy. Electronic address: .
Schembri L; Center of Research in Medical Pharmacology, University of Insubria, Varese, Italy.
Rasini E; Center of Research in Medical Pharmacology, University of Insubria, Varese, Italy.
Pinoli M; Center of Research in Medical Pharmacology, University of Insubria, Varese, Italy.
Scanzano A; Center of Research in Medical Pharmacology, University of Insubria, Varese, Italy.
Luini A; Center of Research in Medical Pharmacology, University of Insubria, Varese, Italy.
Congiu T; Department of Morphological and Surgical Sciences, University of Insubria, Varese, Italy.
Cosentino M; Center of Research in Medical Pharmacology, University of Insubria, Varese, Italy.
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Źródło :
Journal of immunological methods [J Immunol Methods] 2017 Apr; Vol. 443, pp. 55-63. Date of Electronic Publication: 2017 Feb 03.
Typ publikacji :
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Adhesion*/drug effects
Cell Adhesion Molecules/*metabolism
Cell Separation/*methods
Human Umbilical Vein Endothelial Cells/*metabolism
Neutrophils/*metabolism
CD18 Antigens/metabolism ; Cell Shape ; Cells, Cultured ; Centrifugation, Density Gradient ; Coculture Techniques ; E-Selectin/metabolism ; Flow Cytometry ; Human Umbilical Vein Endothelial Cells/drug effects ; Human Umbilical Vein Endothelial Cells/ultrastructure ; Humans ; Intercellular Adhesion Molecule-1/metabolism ; Lipopolysaccharides/pharmacology ; Microscopy, Electron, Scanning ; Neutrophils/ultrastructure ; Phenotype ; Tumor Necrosis Factor-alpha/pharmacology ; Vascular Cell Adhesion Molecule-1/metabolism
Czasopismo naukowe
Tytuł :
Utility of Systematic Isolation of immune cell subsets from HIV-infected individuals for miRNA profiling.
Autorzy :
Bargalló ME; Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic de Barcelona, Barcelona, Spain.
Guardo AC; Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic de Barcelona, Barcelona, Spain.
Maleno MJ; Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic de Barcelona, Barcelona, Spain.
Miralles L; Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic de Barcelona, Barcelona, Spain.
Egaña-Gorroño L; Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic de Barcelona, Barcelona, Spain.
Escribà T; Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic de Barcelona, Barcelona, Spain.
García F; Infectious Diseases Department, Hospital Clínic de Barcelona, Barcelona, Spain.
Gatell JM; Infectious Diseases Department, Hospital Clínic de Barcelona, Barcelona, Spain.
Arnedo M; Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic de Barcelona, Barcelona, Spain.
Plana M; Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic de Barcelona, Barcelona, Spain. Electronic address: .
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Źródło :
Journal of immunological methods [J Immunol Methods] 2017 Mar; Vol. 442, pp. 12-19. Date of Electronic Publication: 2016 Dec 27.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Oligonucleotide Array Sequence Analysis*
Gene Expression Profiling/*methods
HIV Infections/*genetics
Immunomagnetic Separation/*methods
Leukocytes/*chemistry
RNA, Messenger/*genetics
Adult ; Anti-HIV Agents/therapeutic use ; Antigens, CD/blood ; Antigens, CD/immunology ; Case-Control Studies ; Centrifugation, Density Gradient ; Feasibility Studies ; Female ; Flow Cytometry ; Genotype ; HIV Infections/blood ; HIV Infections/drug therapy ; HIV Infections/immunology ; Humans ; Immunophenotyping ; Leukocytes/classification ; Leukocytes/immunology ; Leukocytes/virology ; Male ; Middle Aged ; Phenotype ; RNA Stability ; RNA, Messenger/blood
Czasopismo naukowe
Tytuł :
Superior isolation of antigen-specific brain infiltrating T cells using manual homogenization technique.
Autorzy :
Cumba Garcia LM; Immunology Graduate Program, Mayo Clinic, Rochester, MN, United States.
Huseby Kelcher AM; Immunology Graduate Program, Mayo Clinic, Rochester, MN, United States.
Malo CS; Immunology Graduate Program, Mayo Clinic, Rochester, MN, United States.
Johnson AJ; Department of Immunology, Mayo Clinic, Rochester, MN, United States. Electronic address: .
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Źródło :
Journal of immunological methods [J Immunol Methods] 2016 Dec; Vol. 439, pp. 23-28. Date of Electronic Publication: 2016 Sep 10.
Typ publikacji :
Comparative Study; Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Lymphocyte Activation*
Antigens/*immunology
Brain/*immunology
CD8-Positive T-Lymphocytes/*immunology
Cardiovirus Infections/*immunology
Cell Separation/*methods
Ovalbumin/*immunology
Theilovirus/*immunology
Animals ; Antigens, CD19/immunology ; B-Lymphocytes/immunology ; Brain/metabolism ; Capsid Proteins/immunology ; Cardiovirus Infections/virology ; Cell Survival ; Centrifugation, Density Gradient ; Collagen Type IV/metabolism ; Collagenases/metabolism ; Disease Models, Animal ; Epitopes ; Female ; Flow Cytometry ; Hyaluronan Receptors/immunology ; Male ; Mice, Inbred C57BL ; Povidone/chemistry ; Selectins/immunology ; Silicon Dioxide/chemistry ; Theilovirus/pathogenicity
Czasopismo naukowe
Tytuł :
The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray.
Autorzy :
Bæk R; Department of Clinical Immunology, Part of Extracellular Vesicle Research Center Denmark (EVsearch.dk), Aalborg University Hospital, Aalborg, Denmark. Electronic address: .
Søndergaard EK; Department of Clinical Immunology, Part of Extracellular Vesicle Research Center Denmark (EVsearch.dk), Aalborg University Hospital, Aalborg, Denmark.
Varming K; Department of Clinical Immunology, Part of Extracellular Vesicle Research Center Denmark (EVsearch.dk), Aalborg University Hospital, Aalborg, Denmark.
Jørgensen MM; Department of Clinical Immunology, Part of Extracellular Vesicle Research Center Denmark (EVsearch.dk), Aalborg University Hospital, Aalborg, Denmark.
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Źródło :
Journal of immunological methods [J Immunol Methods] 2016 Nov; Vol. 438, pp. 11-20. Date of Electronic Publication: 2016 Aug 24.
Typ publikacji :
Journal Article
MeSH Terms :
Specimen Handling*
Biomarkers/*blood
Blood Specimen Collection/*methods
Exosomes/*physiology
Protein Array Analysis/*methods
Antibodies/chemistry ; Centrifugation/methods ; Freezing ; Healthy Volunteers ; Humans ; Phenotype ; Temperature ; Transportation
Czasopismo naukowe
Tytuł :
Flow-based sorting of neonatal lymphocyte populations for transcriptomics analysis.
Autorzy :
Misra RS; Department of Pediatrics, Division of Neonatology, University of Rochester Medical Center, Rochester, NY 14642, United States.
Bhattacharya S; Department of Pediatrics, Division of Neonatology, University of Rochester Medical Center, Rochester, NY 14642, United States; Pediatric Molecular and Personalized Medicine Program, University of Rochester Medical Center, Rochester, NY 14642, United States.
Huyck HL; Department of Pediatrics, Division of Neonatology, University of Rochester Medical Center, Rochester, NY 14642, United States.
Wang JC; Rochester Human Immunology Center, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, United States.
Slaunwhite CG; Department of Pediatrics, Division of Neonatology, University of Rochester Medical Center, Rochester, NY 14642, United States.
Slaunwhite SL; Shared Resources Laboratories, University of Rochester Medical Center, Rochester, NY 14642, United States.
Wightman TR; Shared Resources Laboratories, University of Rochester Medical Center, Rochester, NY 14642, United States.
Secor-Socha S; Rochester Human Immunology Center, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, United States.
Misra SK; Department of Pediatrics, Division of Neonatology, University of Rochester Medical Center, Rochester, NY 14642, United States.
Bushnell TP; Shared Resources Laboratories, University of Rochester Medical Center, Rochester, NY 14642, United States.
Reynolds AM; Department of Pediatrics, University at Buffalo, Buffalo, NY 14222, United States.
Ryan RM; Department of Pediatrics, Medical University of South Carolina, Charleston, SC 29425, United States.
Quataert SA; Rochester Human Immunology Center, David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, United States.
Pryhuber GS; Department of Pediatrics, Division of Neonatology, University of Rochester Medical Center, Rochester, NY 14642, United States; Department of Environmental Medicine, University of Rochester Medical Center, Rochester, NY 14642, United States.
Mariani TJ; Department of Pediatrics, Division of Neonatology, University of Rochester Medical Center, Rochester, NY 14642, United States; Pediatric Molecular and Personalized Medicine Program, University of Rochester Medical Center, Rochester, NY 14642, United States.
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Źródło :
Journal of immunological methods [J Immunol Methods] 2016 Oct; Vol. 437, pp. 13-20. Date of Electronic Publication: 2016 Jul 18.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Gene Expression Profiling/*methods
Lymphocytes/*physiology
Cell Separation ; Centrifugation, Density Gradient ; Feasibility Studies ; Ficoll ; Flow Cytometry ; High-Throughput Nucleotide Sequencing ; Humans ; Infant, Newborn ; Lymphocyte Count ; Miniaturization
Czasopismo naukowe
Tytuł :
Novel nasal secretion collection method for the analysis of allergen specific antibodies and inflammatory biomarkers.
Autorzy :
Lü FX; Greer Laboratories Inc., 639 Nuway Circle, P.O. Box 800, Lenoir, NC 28645, United States. />Esch RE
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Źródło :
Journal of immunological methods [J Immunol Methods] 2010 Apr 30; Vol. 356 (1-2), pp. 6-17. Date of Electronic Publication: 2010 Mar 07.
Typ publikacji :
Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Antibody Specificity*
Allergens/*immunology
Centrifugation/*methods
Cytokines/*analysis
Immunoglobulin A/*analysis
Immunoglobulin G/*analysis
Rhinitis, Allergic, Seasonal/*immunology
Adult ; Biomarkers/analysis ; Centrifugation/instrumentation ; Cytokines/immunology ; Female ; Humans ; Immunoglobulin A/immunology ; Immunoglobulin G/immunology ; Male ; Middle Aged
Czasopismo naukowe
Tytuł :
Validation of a commercially available enzyme-linked immunoabsorbent assay for the quantification of human α-Synuclein in cerebrospinal fluid.
Autorzy :
Kruse N; Department of Neuropathology, University Medical Center, Robert-Koch-Str. 40, 37075 Göttingen, Germany. Electronic address: .
Mollenhauer B; Department of Neuropathology, University Medical Center, Robert-Koch-Str. 40, 37075 Göttingen, Germany; Paracelsus-Elena-Klinik, Klinikstr. 16, 34128 Kassel, Germany. Electronic address: .
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Źródło :
Journal of immunological methods [J Immunol Methods] 2015 Nov; Vol. 426, pp. 70-5. Date of Electronic Publication: 2015 Aug 10.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms :
Enzyme-Linked Immunosorbent Assay*
alpha-Synuclein/*cerebrospinal fluid
Biomarkers/cerebrospinal fluid ; Centrifugation ; Freezing ; Humans ; Neurodegenerative Diseases/blood ; Neurodegenerative Diseases/diagnosis ; Observer Variation
Czasopismo naukowe
Tytuł :
Centrifugation facilitates transduction of green fluorescent protein in human monocytes and macrophages by adenovirus at low multiplicity of infection.
Autorzy :
Mayne GC; Department of Microbiology and Infectious Diseases, School of Medicine, Flinders University, GPO Box 2100, Adelaide 5001, Australia.
Borowicz RA
Greeneklee KV
Finlay-Jones JJ
Williams KA
Hart PH
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Źródło :
Journal of immunological methods [J Immunol Methods] 2003 Jul; Vol. 278 (1-2), pp. 45-56.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Centrifugation*/methods
Macrophages/*cytology
Monocytes/*cytology
Transduction, Genetic/*methods
Adenoviridae/genetics ; Animals ; Cell Survival ; Extracellular Space/chemistry ; Flow Cytometry ; Genetic Vectors ; Green Fluorescent Proteins ; Humans ; Integrin alphaVbeta3/biosynthesis ; Integrins/biosynthesis ; Interleukin-1/analysis ; Luminescent Proteins/genetics ; Receptors, Vitronectin/biosynthesis ; Synovial Fluid/cytology ; Tumor Necrosis Factor-alpha/analysis
Czasopismo naukowe
Tytuł :
Effect of isolation protocol on eosinophil function: Percoll gradients versus immunomagnetic beads.
Autorzy :
Sedgwick JB; Department of Medicine, University of Wisconsin, Madison, USA.
Shikama Y
Nagata M
Brener K
Busse WW
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Źródło :
Journal of immunological methods [J Immunol Methods] 1996 Oct 30; Vol. 198 (1), pp. 15-24.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Centrifugation, Density Gradient*
Immunomagnetic Separation*
Povidone*
Silicon Dioxide*
Cell Separation/*methods
Eosinophils/*immunology
Adolescent ; Adult ; Antibodies, Monoclonal/immunology ; Asthma/pathology ; Cell Adhesion/physiology ; Colloids ; Endothelium, Vascular/cytology ; Eosinophils/physiology ; Flow Cytometry ; Humans ; Leukotriene C4/biosynthesis ; Middle Aged ; Receptors, IgG/immunology ; Rhinitis, Allergic, Seasonal/pathology ; Umbilical Veins/cytology
Czasopismo naukowe
Tytuł :
Isolation of human blood dendritic cells by discontinuous Nycodenz gradient centrifugation.
Autorzy :
McLellan AD; Haematology/Immunology Research Laboratory, Christchurch Hospital, New Zealand.
Starling GC
Hart DN
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Źródło :
Journal of immunological methods [J Immunol Methods] 1995 Jul 17; Vol. 184 (1), pp. 81-9.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Centrifugation, Density Gradient*
Dendritic Cells*/immunology
Dendritic Cells*/physiology
Iohexol*
Cell Separation/*methods
Cell Survival ; Humans ; Immunophenotyping
Czasopismo naukowe
Tytuł :
Influences of nasal lavage collection-, processing- and storage methods on inflammatory markers--evaluation of a method for non-invasive sampling of epithelial lining fluid in cystic fibrosis and other respiratory diseases.
Autorzy :
Hentschel J; CF-Center, Pediatrics, Jena University Hospital, Germany. Electronic address: .
Müller U; CF-Center, Pediatrics, Jena University Hospital, Germany.
Doht F; CF-Center, Pediatrics, Jena University Hospital, Germany.
Fischer N; CF-Center, Pediatrics, Jena University Hospital, Germany.
Böer K; Institute for Clinical Chemistry and Laboratory Diagnostics, Jena University Hospital, Germany.
Sonnemann J; Pediatric Haematology and Oncology, Pediatrics, Jena University Hospital, Germany.
Hipler C; Department of Dermatology, Jena University Hospital, Germany.
Hünniger K; Septomics Research Center, Friedrich Schiller University, Germany; Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
Kurzai O; Septomics Research Center, Friedrich Schiller University, Germany; Leibniz Institute for Natural Product Research and Infection Biology - Hans Knoell Institute, Jena, Germany.
Markert UR; Department of Obstetrics, Placenta Laboratory, Jena University Hospital, Germany.
Mainz JG; CF-Center, Pediatrics, Jena University Hospital, Germany.
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Źródło :
Journal of immunological methods [J Immunol Methods] 2014 Feb; Vol. 404, pp. 41-51. Date of Electronic Publication: 2013 Dec 23.
Typ publikacji :
Journal Article
MeSH Terms :
Cystic Fibrosis/*metabolism
Nasal Lavage Fluid/*chemistry
Specimen Handling/*standards
Adolescent ; Adult ; Aged ; Biomarkers/analysis ; Case-Control Studies ; Centrifugation/standards ; Child ; Child, Preschool ; Cystic Fibrosis/diagnosis ; Cystic Fibrosis/immunology ; Enzyme-Linked Immunosorbent Assay ; Female ; Freezing ; Humans ; Inflammation ; Interleukin-1/analysis ; Interleukin-6/analysis ; Interleukin-8/analysis ; Leukocyte Elastase/analysis ; Male ; Middle Aged ; Peroxidase/analysis ; Protease Inhibitors/chemistry ; Tumor Necrosis Factor-alpha/analysis
Czasopismo naukowe
Tytuł :
Induction of low density and up-regulation of CD11b expression of neutrophils and eosinophils by dextran sedimentation and centrifugation.
Autorzy :
Berends C; Department of Allergology, University Hospital Groningen, Netherlands.
Dijkhuizen B
de Monchy JG
Gerritsen J
Kauffman HF
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Źródło :
Journal of immunological methods [J Immunol Methods] 1994 Jan 03; Vol. 167 (1-2), pp. 183-93.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Centrifugation, Density Gradient*
Eosinophils/*physiology
Macrophage-1 Antigen/*biosynthesis
Neutrophils/*physiology
Cell Fractionation ; Dextrans ; Humans ; Lactoferrin/metabolism ; Specific Gravity ; Up-Regulation
Czasopismo naukowe
Tytuł :
An analysis of endothelial microparticles as a function of cell surface antibodies and centrifugation techniques.
Autorzy :
Venable AS; University of North Texas, Applied Physiology Laboratory, Denton, TX, United States.
Williams RR; University of North Texas, Applied Physiology Laboratory, Denton, TX, United States.
Haviland DL; Methodist Research Institute, Core Flow Cytometry Laboratory, Houston, TX, United States.
McFarlin BK; University of North Texas, Applied Physiology Laboratory, Denton, TX, United States; University of North Texas, Dept. of Biology, Denton, TX, United States. Electronic address: .
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Źródło :
Journal of immunological methods [J Immunol Methods] 2014 Apr; Vol. 406, pp. 117-23. Date of Electronic Publication: 2014 Mar 04.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Atherosclerosis/*diagnosis
Cell-Derived Microparticles/*immunology
Endothelial Cells/*immunology
Receptors, Cell Surface/*immunology
Antigens, CD/immunology ; Antigens, Surface/immunology ; Atherosclerosis/immunology ; Biomarkers/blood ; Blood Platelets/cytology ; Cadherins/immunology ; Centrifugation/methods ; Endothelial Cells/cytology ; Endothelium, Vascular/cytology ; Endothelium, Vascular/immunology ; Flow Cytometry ; Humans ; Integrin alphaV/immunology ; Platelet Endothelial Cell Adhesion Molecule-1/immunology ; Platelet Glycoprotein GPIb-IX Complex/immunology ; Staining and Labeling
Czasopismo naukowe
Tytuł :
A high throughput method for enrichment of natural killer cells and lymphocytes and assessment of in vitro cytotoxicity.
Autorzy :
So EC; Division of Research and Development and Surgical Service, Baltimore Veterans Administration Medical Center, 10 N. Greene Street, 5C Surgical Services Area, Baltimore, MD 21201, USA. />Sallin MA
Zhang X
Chan SL
Sahni L
Schulze DH
Davila E
Strome SE
Jain A
Pokaż więcej
Źródło :
Journal of immunological methods [J Immunol Methods] 2013 Aug 30; Vol. 394 (1-2), pp. 40-8. Date of Electronic Publication: 2013 May 13.
Typ publikacji :
Journal Article
MeSH Terms :
Cytotoxicity, Immunologic*
High-Throughput Screening Assays/*methods
Killer Cells, Natural/*immunology
Lymphocytes/*immunology
Centrifugation, Density Gradient ; HT29 Cells ; Humans ; Immunomagnetic Separation ; K562 Cells
Czasopismo naukowe

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