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Tytuł :
Integrated process development: The key to improve Fab production in E. coli.
Autorzy :
Fink M; Christian Doppler Laboratory for production of next-level biopharmaceuticals in E. coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
Schimek C; Christian Doppler Laboratory for production of next-level biopharmaceuticals in E. coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
Cserjan-Puschmann M; Christian Doppler Laboratory for production of next-level biopharmaceuticals in E. coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
Reinisch D; Boehringer Ingelheim RCV GmbH & Co KG, Vienna, Austria.
Brocard C; Boehringer Ingelheim RCV GmbH & Co KG, Vienna, Austria.
Hahn R; Christian Doppler Laboratory for production of next-level biopharmaceuticals in E. coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
Striedner G; Christian Doppler Laboratory for production of next-level biopharmaceuticals in E. coli, Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
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Źródło :
Biotechnology journal [Biotechnol J] 2021 Jun; Vol. 16 (6), pp. e2000562. Date of Electronic Publication: 2021 Mar 01.
Typ publikacji :
Journal Article
MeSH Terms :
Escherichia coli*/genetics
Immunoglobulin Fab Fragments*
Biotechnology ; Centrifugation ; Kinetics
Czasopismo naukowe
Tytuł :
Factors affecting sedimentational separation of bacteria from blood.
Autorzy :
Pitt WG; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Alizadeh M; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Blanco R; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Hunter AK; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Bledsoe CG; Chemical Engineering Department, Brigham Young University, Provo, Utah.
McClellan DS; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Wood ME; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah.
Wood RL; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Ravsten TV; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Hickey CL; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Cameron Beard W; Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah.
Stepan JR; Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah.
Carter A; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Husseini GA; Chemical Engineering Department, American University of Sharjah, Sharjah, United Arab Emirates.
Robison RA; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah.
Welling E; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Torgesen RN; Chemical Engineering Department, Brigham Young University, Provo, Utah.
Anderson CM; Chemical Engineering Department, Brigham Young University, Provo, Utah.
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Źródło :
Biotechnology progress [Biotechnol Prog] 2020 Jan; Vol. 36 (1), pp. e2892. Date of Electronic Publication: 2019 Sep 10.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Centrifugation*
Erythrocytes/*microbiology
Escherichia coli/*isolation & purification
Humans ; Particle Size
Czasopismo naukowe
Tytuł :
Towards safer stable isotope probing - effect of formamide on the separation of isotope-labeled and unlabeled Escherichia coli RNA by isopycnic density ultracentrifugation.
Autorzy :
Weis S; Faculty of Medical and Life Sciences, Institute of Precision Medicine, Microbiology and Hygiene Group, Furtwangen University, Villingen-Schwenningen, Germany.
Schnell S; Institute of Applied Microbiology, Research Center for BioSystems, Land Use, and Nutrition (IFZ), Justus-Liebig-University Giessen, Germany.
Egert M; Faculty of Medical and Life Sciences, Institute of Precision Medicine, Microbiology and Hygiene Group, Furtwangen University, Villingen-Schwenningen, Germany.
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Źródło :
Canadian journal of microbiology [Can J Microbiol] 2020 Aug; Vol. 66 (8), pp. 491-494. Date of Electronic Publication: 2020 Mar 05.
Typ publikacji :
Journal Article
MeSH Terms :
Escherichia coli/*genetics
RNA, Bacterial/*genetics
RNA, Bacterial/*isolation & purification
Carbon Isotopes/chemistry ; Centrifugation, Density Gradient/methods ; Escherichia coli/chemistry ; Formamides/chemistry ; Isotope Labeling/methods ; RNA, Bacterial/chemistry ; Ultracentrifugation/methods
Czasopismo naukowe
Tytuł :
Re-introducing non-optimal synonymous codons into codon-optimized constructs enhances soluble recovery of recombinant proteins from Escherichia coli.
Autorzy :
Konczal J; Drug Discovery Program, CRUK Beatson Institute, Glasgow, United Kingdom.
Bower J; Drug Discovery Program, CRUK Beatson Institute, Glasgow, United Kingdom.
Gray CH; Drug Discovery Program, CRUK Beatson Institute, Glasgow, United Kingdom.
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Źródło :
PloS one [PLoS One] 2019 Apr 23; Vol. 14 (4), pp. e0215892. Date of Electronic Publication: 2019 Apr 23 (Print Publication: 2019).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Codon/*genetics
Escherichia coli/*genetics
Recombinant Proteins/*genetics
Ribosomes/*genetics
Amino Acid Sequence/genetics ; Centrifugation ; Guanosine Triphosphate/genetics ; Protein Biosynthesis/genetics ; RNA, Transfer/genetics ; Recombinant Proteins/chemistry ; Solubility
Czasopismo naukowe
Tytuł :
Effect of rotor type on the separation of isotope-labeled and unlabeled Escherichia coli RNA by isopycnic density ultracentrifugation.
Autorzy :
Herrmann E; a Faculty of Medical and Life Sciences, Institute of Precision Medicine, Microbiology & Hygiene Group, Furtwangen University, Campus Villingen-Schwenningen, Germany.
Koch P; a Faculty of Medical and Life Sciences, Institute of Precision Medicine, Microbiology & Hygiene Group, Furtwangen University, Campus Villingen-Schwenningen, Germany.
Riedel CU; b Institute of Microbiology and Biotechnology, University of Ulm, 89069 Ulm, Germany.
Young W; c AgResearch Ltd., Grasslands Research Centre, Palmerston North 4442, New Zealand.
Egert M; a Faculty of Medical and Life Sciences, Institute of Precision Medicine, Microbiology & Hygiene Group, Furtwangen University, Campus Villingen-Schwenningen, Germany.
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Źródło :
Canadian journal of microbiology [Can J Microbiol] 2017 Jan; Vol. 63 (1), pp. 83-87. Date of Electronic Publication: 2016 Sep 27.
Typ publikacji :
Evaluation Study; Journal Article
MeSH Terms :
Centrifugation, Density Gradient/*methods
Centrifugation, Isopycnic/*methods
Escherichia coli/*chemistry
RNA, Bacterial/*isolation & purification
Carbon Isotopes/chemistry ; Carbon Isotopes/metabolism ; Centrifugation, Density Gradient/instrumentation ; Centrifugation, Isopycnic/instrumentation ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Isotope Labeling ; RNA, Bacterial/chemistry ; RNA, Bacterial/genetics ; RNA, Bacterial/metabolism
Czasopismo naukowe
Tytuł :
Iron-sulfur cluster carrier proteins involved in the assembly of Escherichia coli NADH:ubiquinone oxidoreductase (complex I).
Autorzy :
Burschel S; Albert-Ludwigs-Universität, Institut für Biochemie, Albertstr. 21, D-79104, Freiburg, Germany.
Kreuzer Decovic D; Albert-Ludwigs-Universität, Institut für Biochemie, Albertstr. 21, D-79104, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Germany.
Nuber F; Albert-Ludwigs-Universität, Institut für Biochemie, Albertstr. 21, D-79104, Freiburg, Germany.
Stiller M; Albert-Ludwigs-Universität, Institut für Biochemie, Albertstr. 21, D-79104, Freiburg, Germany.
Hofmann M; Albert-Ludwigs-Universität, Institut für Biochemie, Albertstr. 21, D-79104, Freiburg, Germany.
Zupok A; University of Potsdam, Institut für Biochemie und Biologie, Karl-Liebknecht-Str. 24-25, 14476, Potsdam-Golm, Germany.
Siemiatkowska B; Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany.
Gorka M; Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam-Golm, Germany.
Leimkühler S; University of Potsdam, Institut für Biochemie und Biologie, Karl-Liebknecht-Str. 24-25, 14476, Potsdam-Golm, Germany.
Friedrich T; Albert-Ludwigs-Universität, Institut für Biochemie, Albertstr. 21, D-79104, Freiburg, Germany.; Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Germany.
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Źródło :
Molecular microbiology [Mol Microbiol] 2019 Jan; Vol. 111 (1), pp. 31-45. Date of Electronic Publication: 2018 Oct 23.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Protein Multimerization*
Carrier Proteins/*metabolism
Electron Transport Complex I/*metabolism
Escherichia coli/*enzymology
Escherichia coli/*metabolism
Escherichia coli Proteins/*metabolism
Iron-Sulfur Proteins/*metabolism
Centrifugation, Density Gradient ; Electron Spin Resonance Spectroscopy ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli/genetics ; Gene Deletion
Czasopismo naukowe
Tytuł :
Rapid separation of bacteria from blood - Chemical aspects.
Autorzy :
Alizadeh M; Chemical Engineering Department, Brigham Young University, Provo, UT 84602, United States.
Wood RL; Chemical Engineering Department, Brigham Young University, Provo, UT 84602, United States.
Buchanan CM; Chemical Engineering Department, Brigham Young University, Provo, UT 84602, United States.
Bledsoe CG; Chemical Engineering Department, Brigham Young University, Provo, UT 84602, United States.
Wood ME; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, United States.
McClellan DS; Chemical Engineering Department, Brigham Young University, Provo, UT 84602, United States.
Blanco R; Chemical Engineering Department, Brigham Young University, Provo, UT 84602, United States.
Ravsten TV; Chemical Engineering Department, Brigham Young University, Provo, UT 84602, United States.
Husseini GA; Chemical Engineering Department, American University of Sharjah, Sharjah, United Arab Emirates.
Hickey CL; Chemical Engineering Department, Brigham Young University, Provo, UT 84602, United States.
Robison RA; Department of Microbiology and Molecular Biology, Brigham Young University, Provo, UT 84602, United States.
Pitt WG; Chemical Engineering Department, Brigham Young University, Provo, UT 84602, United States. Electronic address: .
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Źródło :
Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2017 Jun 01; Vol. 154, pp. 365-372. Date of Electronic Publication: 2017 Mar 16.
Typ publikacji :
Journal Article
MeSH Terms :
Equipment Design*
Cell Separation/*instrumentation
Centrifugation/*instrumentation
Escherichia coli/*isolation & purification
Anticoagulants/pharmacology ; Bacteremia/blood ; Blood Platelets/cytology ; Blood Platelets/drug effects ; Cell Separation/methods ; Centrifugation/methods ; Citrates/pharmacology ; Edetic Acid/pharmacology ; Erythrocytes/cytology ; Erythrocytes/drug effects ; Heparin/pharmacology ; Humans ; Leukocytes/cytology ; Leukocytes/drug effects ; Models, Biological ; Sodium Citrate
Czasopismo naukowe
Tytuł :
Evaluation of options for harvest of a recombinant E. Coli fermentation producing a domain antibody using ultra scale-down techniques and pilot-scale verification.
Autorzy :
Voulgaris I; Dept. of Biochemical Engineering, The Advanced Centre for Biochemical Engineering, UCL, Gower St, London, WC1E 6BT.; Biopharm Process Research, BioPharm R&D, GlaxoSmithKline, R&D, Stevenage, SG1 2NY.
Chatel A; Dept. of Biochemical Engineering, The Advanced Centre for Biochemical Engineering, UCL, Gower St, London, WC1E 6BT.
Hoare M; Dept. of Biochemical Engineering, The Advanced Centre for Biochemical Engineering, UCL, Gower St, London, WC1E 6BT.
Finka G; Biopharm Process Research, BioPharm R&D, GlaxoSmithKline, R&D, Stevenage, SG1 2NY.
Uden M; Biopharm Process Research, BioPharm R&D, GlaxoSmithKline, R&D, Stevenage, SG1 2NY.
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Źródło :
Biotechnology progress [Biotechnol Prog] 2016 Mar; Vol. 32 (2), pp. 382-92. Date of Electronic Publication: 2016 Jan 12.
Typ publikacji :
Journal Article
MeSH Terms :
Centrifugation*
Fermentation*
Filtration*
Antibodies/*metabolism
Escherichia coli/*metabolism
Antibodies/chemistry ; Cell Separation ; Escherichia coli/cytology
Czasopismo naukowe
Tytuł :
Physiologically distinct subpopulations formed in Escherichia coli cultures in response to heat shock.
Autorzy :
Bruhn-Olszewska B; Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Szczepaniak P; Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Matuszewska E; Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Kuczyńska-Wiśnik D; Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Stojowska-Swędrzyńska K; Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Moruno Algara M; Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland.
Laskowska E; Department of General and Medical Biochemistry, Faculty of Biology, University of Gdansk, Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland. Electronic address: .
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Źródło :
Microbiological research [Microbiol Res] 2018 Apr; Vol. 209, pp. 33-42. Date of Electronic Publication: 2018 Feb 13.
Typ publikacji :
Journal Article
MeSH Terms :
Escherichia coli*/classification
Escherichia coli*/growth & development
Escherichia coli*/metabolism
Heat-Shock Response/*physiology
Centrifugation, Density Gradient ; Hydrogen Peroxide/pharmacology ; Oxidation-Reduction ; Oxidative Stress/physiology ; Protein Aggregates ; Ribosomes/metabolism
Czasopismo naukowe
Tytuł :
Removal of protein S1 from Escherichia coli ribosomes without the use of affinity chromatography.
Autorzy :
Baranovskaya MD; Institute of Protein Research of the Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
Ugarov VI; Institute of Protein Research of the Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
Chetverina HV; Institute of Protein Research of the Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
Chetverin AB; Institute of Protein Research of the Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia. Electronic address: .
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Źródło :
Analytical biochemistry [Anal Biochem] 2017 Jan 15; Vol. 517, pp. 53-55. Date of Electronic Publication: 2016 Nov 16.
Typ publikacji :
Journal Article
MeSH Terms :
Escherichia coli/*chemistry
Escherichia coli Proteins/*isolation & purification
Ribosomal Proteins/*isolation & purification
Ribosomes/*chemistry
Centrifugation, Density Gradient/methods ; Chromatography, Affinity ; Poly U/chemistry ; Sucrose/analogs & derivatives ; Sucrose/chemistry
Czasopismo naukowe
Tytuł :
Growth phase dependent changes in the structure and protein composition of nucleoid in Escherichia coli.
Autorzy :
Talukder A; Department of Microbiology, Jahangirnagar University, Dhaka, 1342, Bangladesh. .; Micro-Nano Technology Research Center, Hosei University, Tokyo, 184-0003, Japan. .
Ishihama A; Department of Molecular Genetics, National Institute of Genetics, Shizuoka, 411-8540, Japan.; Micro-Nano Technology Research Center, Hosei University, Tokyo, 184-0003, Japan.
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Źródło :
Science China. Life sciences [Sci China Life Sci] 2015 Sep; Vol. 58 (9), pp. 902-11. Date of Electronic Publication: 2015 Jul 24.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Escherichia coli/*growth & development
Escherichia coli/*metabolism
Escherichia coli Proteins/*metabolism
Antibodies/chemistry ; Bacterial Outer Membrane Proteins/metabolism ; Centrifugation, Density Gradient ; DNA, Bacterial/genetics ; DNA-Binding Proteins/metabolism ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli/genetics ; Factor For Inversion Stimulation Protein ; Genomics ; Host Factor 1 Protein/metabolism ; Microscopy, Fluorescence ; Molecular Chaperones/metabolism ; Phenotype ; Sucrose/chemistry
Czasopismo naukowe
Tytuł :
A simple separation method for downstream biochemical analysis of aquatic microbes.
Autorzy :
Garrison CE; Old Dominion University, Department of Ocean, Earth and Atmospheric Sciences, 4600 Elkhorn Ave., Norfolk, VA 23529, USA. Electronic address: .
Bochdansky AB; Old Dominion University, Department of Ocean, Earth and Atmospheric Sciences, 4600 Elkhorn Ave., Norfolk, VA 23529, USA. Electronic address: .
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Źródło :
Journal of microbiological methods [J Microbiol Methods] 2015 Apr; Vol. 111, pp. 78-86. Date of Electronic Publication: 2015 Feb 02.
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Water Microbiology*
Chemical Fractionation/*methods
Escherichia coli/*chemistry
Escherichia coli/*isolation & purification
Euglenozoa/*chemistry
Euglenozoa/*isolation & purification
Stramenopiles/*chemistry
Stramenopiles/*isolation & purification
Aquatic Organisms/chemistry ; Aquatic Organisms/isolation & purification ; Centrifugation, Density Gradient ; Diatoms/chemistry ; Diatoms/isolation & purification ; Filtration/methods ; Lipids/isolation & purification ; Nucleic Acids/isolation & purification ; Polysaccharides, Bacterial/isolation & purification ; Proteins/isolation & purification
Czasopismo naukowe
Tytuł :
Integration of host strain bioengineering and bioprocess development using ultra-scale down studies to select the optimum combination: an antibody fragment primary recovery case study.
Autorzy :
Aucamp JP; Bioprocess Research and Development, Novartis Phama AG, Basel, Switzerland. .
Davies R
Hallet D
Weiss A
Titchener-Hooker NJ
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Źródło :
Biotechnology and bioengineering [Biotechnol Bioeng] 2014 Oct; Vol. 111 (10), pp. 1971-81. Date of Electronic Publication: 2014 Jun 04.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Escherichia coli/*genetics
Immunoglobulin Fab Fragments/*genetics
Immunoglobulin Fab Fragments/*isolation & purification
Industrial Microbiology/*instrumentation
Bioengineering/instrumentation ; Bioreactors ; Centrifugation ; Equipment Design ; Escherichia coli/cytology ; Immunoglobulin Fragments
Czasopismo naukowe
Tytuł :
Characterization of the RNase R association with ribosomes.
Autorzy :
Malecki M
Bárria C
Arraiano CM; Instituto de Tecnologia Química e Biológica (ITQB), Oeiras, Portugal. .
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Źródło :
BMC microbiology [BMC Microbiol] 2014 Feb 11; Vol. 14, pp. 34. Date of Electronic Publication: 2014 Feb 11.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Escherichia coli/*enzymology
Escherichia coli/*metabolism
Escherichia coli Proteins/*metabolism
Exoribonucleases/*metabolism
Ribosomes/*metabolism
Blotting, Western ; Centrifugation, Density Gradient ; Escherichia coli/growth & development ; Escherichia coli/radiation effects ; Protein Binding ; Temperature
Czasopismo naukowe
Tytuł :
Amplification and purification of T4-like escherichia coli phages for phage therapy: from laboratory to pilot scale.
Autorzy :
Bourdin G; Nestlé Research Centre, Nestec Ltd, Vers-chez-les-Blanc, Lausanne, Switzerland.
Schmitt B
Marvin Guy L
Germond JE
Zuber S
Michot L
Reuteler G
Brüssow H
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Źródło :
Applied and environmental microbiology [Appl Environ Microbiol] 2014 Feb; Vol. 80 (4), pp. 1469-76. Date of Electronic Publication: 2013 Dec 20.
Typ publikacji :
Journal Article
MeSH Terms :
Bacteriophage T4/*growth & development
Bacteriophage T4/*isolation & purification
Biological Therapy/*methods
Escherichia coli/*virology
Centrifugation/methods ; Drug Stability ; Drug Storage ; Filtration/methods ; Mass Spectrometry ; Microscopy, Electron ; Virology/methods
Czasopismo naukowe
Tytuł :
An ultra scale-down approach to study the interaction of fermentation, homogenization, and centrifugation for antibody fragment recovery from rec E. coli.
Autorzy :
Li Q; Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, United Kingdom. />Mannall GJ
Ali S
Hoare M
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Źródło :
Biotechnology and bioengineering [Biotechnol Bioeng] 2013 Aug; Vol. 110 (8), pp. 2150-60. Date of Electronic Publication: 2013 May 07.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Biotechnology/*methods
Escherichia coli/*metabolism
Immunoglobulin Fragments/*isolation & purification
Centrifugation/methods ; Fermentation ; Recombinant Proteins/isolation & purification
Czasopismo naukowe
Tytuł :
In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation.
Autorzy :
Jewett MC; Department of Genetics, Harvard Medical School, Boston, MA 02115, USA. />Fritz BR
Timmerman LE
Church GM
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Źródło :
Molecular systems biology [Mol Syst Biol] 2013 Jun 25; Vol. 9, pp. 678. Date of Electronic Publication: 2013 Jun 25.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Genetic Engineering*
Escherichia coli/*genetics
RNA, Ribosomal, 23S/*genetics
Ribosomal Proteins/*genetics
Ribosomes/*genetics
Centrifugation, Density Gradient ; Clindamycin/pharmacology ; Drug Resistance, Bacterial/genetics ; Escherichia coli/chemistry ; Escherichia coli/drug effects ; Escherichia coli/metabolism ; Mutation ; Protein Synthesis Inhibitors/pharmacology ; RNA, Ribosomal, 23S/metabolism ; Ribosomal Proteins/metabolism ; Ribosomes/chemistry ; Ribosomes/drug effects ; Ribosomes/metabolism ; Transcription, Genetic
Czasopismo naukowe
Tytuł :
Human CCT4 and CCT5 chaperonin subunits expressed in Escherichia coli form biologically active homo-oligomers.
Autorzy :
Sergeeva OA; Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Chen B
Haase-Pettingell C
Ludtke SJ
Chiu W
King JA
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Źródło :
The Journal of biological chemistry [J Biol Chem] 2013 Jun 14; Vol. 288 (24), pp. 17734-44. Date of Electronic Publication: 2013 Apr 23.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Escherichia coli*
Chaperonin Containing TCP-1/*chemistry
Adenosine Triphosphate/chemistry ; Centrifugation, Density Gradient ; Chaperonin 60/ultrastructure ; Chaperonin Containing TCP-1/biosynthesis ; Chaperonin Containing TCP-1/isolation & purification ; Chaperonin Containing TCP-1/ultrastructure ; Chromatography, Gel ; Cryoelectron Microscopy ; Humans ; Hydrolysis ; Luciferases/chemistry ; Protein Multimerization ; Protein Refolding ; Protein Stability ; Protein Structure, Quaternary ; Protein Structure, Secondary ; Protein Subunits/biosynthesis ; Protein Subunits/chemistry ; Serum Albumin, Bovine/chemistry ; Transition Temperature ; gamma-Crystallins/chemistry
Czasopismo naukowe
Tytuł :
Screening and identification of genetic loci involved in producing more/denser inclusion bodies in Escherichia coli.
Autorzy :
Pandey N; BioChemical Sciences & Engineering, E.I DuPont India Pvt. Ltd. DuPont Knowledge Centre, Survey No 542/2. DS-9, ICICI Knowledge Park, Ranga Reddy District, Turkapally, Hyderabad 500078, India.
Sachan A
Chen Q
Ruebling-Jass K
Bhalla R
Panguluri KK
Rouviere PE
Cheng Q
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Źródło :
Microbial cell factories [Microb Cell Fact] 2013 May 02; Vol. 12, pp. 43. Date of Electronic Publication: 2013 May 02.
Typ publikacji :
Journal Article
MeSH Terms :
Escherichia coli/*metabolism
Inclusion Bodies/*metabolism
Centrifugation, Density Gradient ; Escherichia coli/genetics ; Escherichia coli/growth & development ; Escherichia coli Proteins/genetics ; Gene Knockout Techniques ; Gene Library ; Genetic Loci ; Povidone/chemistry ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics ; Silicon Dioxide/chemistry
Czasopismo naukowe
Tytuł :
Protein interaction studies point to new functions for Escherichia coli glyceraldehyde-3-phosphate dehydrogenase.
Autorzy :
Ferreira E; Departament de Bioquímica i Biología Molecular, Facultat de Farmàcia, Institut de Biomedicina de la Universitat de Barcelona (IBUB), Universitat de Barcelona, Av. Diagonal, 643, E-08028 Barcelona, Spain. />Giménez R
Aguilera L
Guzmán K
Aguilar J
Badia J
Baldomà L
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Źródło :
Research in microbiology [Res Microbiol] 2013 Feb-Mar; Vol. 164 (2), pp. 145-54. Date of Electronic Publication: 2012 Nov 27.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Protein Interaction Mapping*
Escherichia coli/*enzymology
Escherichia coli Proteins/*metabolism
Glyceraldehyde-3-Phosphate Dehydrogenases/*metabolism
Centrifugation ; Cross-Linking Reagents/metabolism ; Formaldehyde/metabolism ; Immunoblotting ; Mass Spectrometry ; Protein Binding
Czasopismo naukowe

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