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Tytuł:
Development of a mobile laboratory system in hydrogen fuel cell buses and evaluation of the performance for COVID-19 RT-PCR testing.
Autorzy:
Okude M; LSI Medience Corporation, 3-30-1 Shimura, Itabashi, Tokyo, 174-8555, Japan.
Suzuki K; Center for Cybernics Research, University of Tsukuba, 1-1-1 Tennodai, TsukubaTsukuba, Ibaraki, 305-8573, Japan.
Naito A; LSI Medience Corporation, 3-30-1 Shimura, Itabashi, Tokyo, 174-8555, Japan.
Ebashi A; Department of Infectious Diseases, University of Tsukuba Hospital, 2-1-1 Amakubo, Tsukuba, Ibaraki, 305-8576, Japan.
Kusama T; Department of Infectious Diseases, University of Tsukuba Hospital, 2-1-1 Amakubo, Tsukuba, Ibaraki, 305-8576, Japan.
Kiyotaki J; Miroku Medical Laboratory Inc, 659-2 Innai, Saku, Nagano, 384-2201, Japan.; Department of Clinical Laboratory, Tsukuba Medical Center Hospital, 1-3-1 Amakubo, Tsukuba, Ibaraki, 305-8558, Japan.
Akashi Y; Department of Infectious Diseases, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
Kiyasu Y; Department of Infectious Diseases, University of Tsukuba Hospital, 2-1-1 Amakubo, Tsukuba, Ibaraki, 305-8576, Japan.; Department of Infectious Diseases, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.; Division of Infectious Diseases, Department of Medicine, Tsukuba Medical Center Hospital, 1-3-1 Amakubo Tsukuba, Ibaraki, 305-8558, Japan.
Kurihara Y; Department of Infectious Diseases, University of Tsukuba Hospital, 2-1-1 Amakubo, Tsukuba, Ibaraki, 305-8576, Japan.
Notake S; Department of Clinical Laboratory, Tsukuba Medical Center Hospital, 1-3-1 Amakubo, Tsukuba, Ibaraki, 305-8558, Japan.
Takanashi M; LSI Medience Corporation, 3-30-1 Shimura, Itabashi, Tokyo, 174-8555, Japan.
Setoyama T; LSI Medience Corporation, 3-30-1 Shimura, Itabashi, Tokyo, 174-8555, Japan.
Kawakami Y; Department of Laboratory Medicine, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan.
Suzuki H; Department of Infectious Diseases, University of Tsukuba Hospital, 2-1-1 Amakubo, Tsukuba, Ibaraki, 305-8576, Japan. .; Department of Infectious Diseases, Faculty of Medicine, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8575, Japan. .
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Źródło:
Scientific reports [Sci Rep] 2023 Oct 16; Vol. 13 (1), pp. 17546. Date of Electronic Publication: 2023 Oct 16.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19*/diagnosis
Humans ; SARS-CoV-2/genetics ; COVID-19 Nucleic Acid Testing ; COVID-19 Testing ; Motor Vehicles ; Sensitivity and Specificity
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Degradation of SARS-CoV-2 specific ribonucleic acid in samples for nucleic acid amplification detection.
Autorzy:
Takeuchi K; Institute of Education, Innovative Human Resource Development Division, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan.; Department of Genetics, Hyogo College of Medicine, Nishinomiya, Hyogo, Japan.
Yanagisawa H; Central Chemical Laboratory, SGS Japan Inc., Hodogaya-ku, Yokohama, Japan.
Kurosawa Y; Central Chemical Laboratory, SGS Japan Inc., Hodogaya-ku, Yokohama, Japan.
Iida Y; Institute of Education, Innovative Human Resource Development Division, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, Japan.
Kawai K; Central Chemical Laboratory, SGS Japan Inc., Hodogaya-ku, Yokohama, Japan.
Fujimaki S; Central Chemical Laboratory, SGS Japan Inc., Hodogaya-ku, Yokohama, Japan.
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Źródło:
PloS one [PLoS One] 2022 Mar 11; Vol. 17 (3), pp. e0264541. Date of Electronic Publication: 2022 Mar 11 (Print Publication: 2022).
Typ publikacji:
Journal Article
MeSH Terms:
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19 Nucleic Acid Testing*
Nucleic Acid Amplification Techniques*
RNA Stability*
RNA, Viral/*genetics
SARS-CoV-2/*genetics
Humans
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Single-tube collection and nucleic acid analysis of clinical samples for SARS-CoV-2 saliva testing.
Autorzy:
Cole KH; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697, USA.
Bouin A; Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA, 92697, USA.
Ruiz C; Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, 92697, USA.
Semler BL; Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA, 92697, USA.
Inlay MA; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697, USA.; Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA, 92697, USA.
Lupták A; Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA, 92697, USA. .; Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, 92697, USA. .; Department of Chemistry, University of California, Irvine, Irvine, CA, 92697, USA. .
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Źródło:
Scientific reports [Sci Rep] 2022 Mar 10; Vol. 12 (1), pp. 3951. Date of Electronic Publication: 2022 Mar 10.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
COVID-19 Nucleic Acid Testing/*methods
SARS-CoV-2/*genetics
Saliva/*virology
COVID-19 Nucleic Acid Testing/instrumentation ; Humans ; Molecular Diagnostic Techniques ; Nucleic Acid Amplification Techniques ; Real-Time Polymerase Chain Reaction ; Specimen Handling/instrumentation ; Specimen Handling/methods
SCR Protocol:
LAMP assay
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples.
Autorzy:
Papadakis G; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 100 N. Plastira Str., 70013, Heraklion, Greece. .; BIOPIX DNA TECHNOLOGY PC, Science and Technology Park of Crete, 100 N. Plastira Str., 70013, Heraklion, Greece. .
Pantazis AK; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 100 N. Plastira Str., 70013, Heraklion, Greece.; BIOPIX DNA TECHNOLOGY PC, Science and Technology Park of Crete, 100 N. Plastira Str., 70013, Heraklion, Greece.
Fikas N; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 100 N. Plastira Str., 70013, Heraklion, Greece.; BIOPIX DNA TECHNOLOGY PC, Science and Technology Park of Crete, 100 N. Plastira Str., 70013, Heraklion, Greece.
Chatziioannidou S; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 100 N. Plastira Str., 70013, Heraklion, Greece.; BIOPIX DNA TECHNOLOGY PC, Science and Technology Park of Crete, 100 N. Plastira Str., 70013, Heraklion, Greece.; Department of Biology, University of Crete, 70013, Voutes, Heraklion, Greece.
Tsiakalou V; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 100 N. Plastira Str., 70013, Heraklion, Greece.
Michaelidou K; Laboratory of Translational Oncology, School of Medicine, University of Crete, 71500, Heraklion, Greece.
Pogka V; National SARS-CoV-2 Reference Laboratory, Hellenic Pasteur Institute, 127 Vas. Sofias Ave., 11521, Athens, Greece.
Megariti M; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 100 N. Plastira Str., 70013, Heraklion, Greece.
Vardaki M; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 100 N. Plastira Str., 70013, Heraklion, Greece.; Department of Biology, University of Crete, 70013, Voutes, Heraklion, Greece.
Giarentis K; Department of Biology, University of Crete, 70013, Voutes, Heraklion, Greece.
Heaney J; Advanced Pathogens Diagnostics Unit, University College London Hospitals NHS Trust, London, WC1H 9AX, UK.; UCL Great Ormond Street Institute of Child Health, London, UK.
Nastouli E; Advanced Pathogens Diagnostics Unit, University College London Hospitals NHS Trust, London, WC1H 9AX, UK.; UCL Great Ormond Street Institute of Child Health, London, UK.
Karamitros T; National SARS-CoV-2 Reference Laboratory, Hellenic Pasteur Institute, 127 Vas. Sofias Ave., 11521, Athens, Greece.
Mentis A; National SARS-CoV-2 Reference Laboratory, Hellenic Pasteur Institute, 127 Vas. Sofias Ave., 11521, Athens, Greece.
Zafiropoulos A; Laboratory of Clinical Virology, School of Medicine, University of Crete, 71500, Heraklion, Greece.
Sourvinos G; Laboratory of Clinical Virology, School of Medicine, University of Crete, 71500, Heraklion, Greece.
Agelaki S; Department of Biology, University of Crete, 70013, Voutes, Heraklion, Greece.; Department of Medical Oncology, University General Hospital, 71110, Heraklion, Greece.
Gizeli E; Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, 100 N. Plastira Str., 70013, Heraklion, Greece. .; Department of Biology, University of Crete, 70013, Voutes, Heraklion, Greece. .
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Źródło:
Scientific reports [Sci Rep] 2022 Mar 08; Vol. 12 (1), pp. 3775. Date of Electronic Publication: 2022 Mar 08.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
COVID-19/virology ; COVID-19 Nucleic Acid Testing/instrumentation ; Colorimetry ; Humans ; Limit of Detection ; Molecular Diagnostic Techniques ; Nasopharynx/virology ; Neoplasms/diagnosis ; Neoplasms/genetics ; Neoplasms/pathology ; Nucleic Acid Amplification Techniques ; Point-of-Care Testing ; Proto-Oncogene Proteins B-raf/genetics ; RNA, Viral/analysis ; RNA, Viral/metabolism ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; Sensitivity and Specificity ; Smartphone
SCR Protocol:
LAMP assay
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
The association between real-time reverse transcriptase polymerase chain reaction cycle threshold values, symptoms and disease severity among COVID-19 patients in the community: a retrospective cohort study.
Autorzy:
Makov-Assif M; Innovation Division, Clalit Research Institute, Clalit Health Services, Tel Aviv, Israel.
Krispin S; Innovation Division, Clalit Research Institute, Clalit Health Services, Tel Aviv, Israel.
Ben-Shlomo Y; Innovation Division, Clalit Research Institute, Clalit Health Services, Tel Aviv, Israel.
Holander T; Innovation Division, Clalit Research Institute, Clalit Health Services, Tel Aviv, Israel.
Dagan N; Innovation Division, Clalit Research Institute, Clalit Health Services, Tel Aviv, Israel.; Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA.
Balicer R; Innovation Division, Clalit Research Institute, Clalit Health Services, Tel Aviv, Israel.; Faculty of Health Sciences, School of Public Health, Ben Gurion University of the Negev, Sheva, Be'er, Israel.
Barda N; Innovation Division, Clalit Research Institute, Clalit Health Services, Tel Aviv, Israel.; Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA.
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Źródło:
Infectious diseases (London, England) [Infect Dis (Lond)] 2022 Mar; Vol. 54 (3), pp. 205-212. Date of Electronic Publication: 2021 Nov 07.
Typ publikacji:
Journal Article
MeSH Terms:
COVID-19*/diagnosis
COVID-19 Nucleic Acid Testing*
Hospitalization ; Humans ; Models, Theoretical ; Real-Time Polymerase Chain Reaction ; Retrospective Studies ; Reverse Transcriptase Polymerase Chain Reaction ; Severity of Illness Index
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Investigation of discordant SARS-CoV-2 RT-PCR results using minimally processed saliva.
Autorzy:
White D; Biointerfaces Institute, McMaster University, Hamilton, ON, L8S 4L8, Canada.
Gu J; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, L8N 3Z5, Canada.
Steinberg CJ; Department of Medicine, McMaster University, Hamilton, ON, L8N 3Z5, Canada.
Yamamura D; Michael DeGroote Institute of Infectious Disease Research, McMaster University, Hamilton, ON, L8N 3Z5, Canada.; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, L8N 3Z5, Canada.
Salena BJ; Department of Medicine, McMaster University, Hamilton, ON, L8N 3Z5, Canada.
Balion C; Department of Pathology and Molecular Medicine, McMaster University, Hamilton, ON, L8N 3Z5, Canada.
Filipe CDM; Department of Chemical Engineering, McMaster University, Hamilton, ON, L8S 4M1, Canada.
Capretta A; Biointerfaces Institute, McMaster University, Hamilton, ON, L8S 4L8, Canada.
Li Y; Biointerfaces Institute, McMaster University, Hamilton, ON, L8S 4L8, Canada. .; Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON, L8N 3Z5, Canada. .; Michael DeGroote Institute of Infectious Disease Research, McMaster University, Hamilton, ON, L8N 3Z5, Canada. .; School of Biomedical Engineering, McMaster University, Hamilton, ON, L8S 4K1, Canada. .
Brennan JD; Biointerfaces Institute, McMaster University, Hamilton, ON, L8S 4L8, Canada. .
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Źródło:
Scientific reports [Sci Rep] 2022 Feb 18; Vol. 12 (1), pp. 2806. Date of Electronic Publication: 2022 Feb 18.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19 Nucleic Acid Testing*
SARS-CoV-2/*isolation & purification
Saliva/*virology
False Negative Reactions ; Healthy Volunteers ; Humans ; Point-of-Care Testing
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Self-reported symptom study of COVID-19 chemosensory dysfunction in Malaysia.
Autorzy:
Lee SH; Department of Otorhinolaryngology, Hospital Sultanah Bahiyah, KM 6 Jalan Langgar, Alor Setar, 05450, Kedah, Malaysia. .
Yeoh ZX; Department of Otorhinolaryngology, Hospital Sultanah Bahiyah, KM 6 Jalan Langgar, Alor Setar, 05450, Kedah, Malaysia.
Sachlin IS; Department of Otorhinolaryngology, Hospital Sultanah Bahiyah, KM 6 Jalan Langgar, Alor Setar, 05450, Kedah, Malaysia.
Gazali N; Department of Otorhinolaryngology, Hospital Sultanah Bahiyah, KM 6 Jalan Langgar, Alor Setar, 05450, Kedah, Malaysia.
Soelar SA; Clinical Research Centre, Hospital Sultanah Bahiyah, Kedah, Malaysia.
Foo CY; Real World Insights, IQVIA Asia Pacific, Petaling Jaya, Malaysia.
Low LL; Department of Medicine, Hospital Sultanah Bahiyah, Kedah, Malaysia.
Syed Alwi SB; Department of Medicine, Hospital Sultanah Bahiyah, Kedah, Malaysia.
Tengku Kamalden TMI; Department of Otorhinolaryngology, Hospital Sultan Ismail, Johor, Malaysia.
Shanmuganathan J; Department of Otorhinolaryngology, Hospital Sultanah Aminah, Johor, Malaysia.
Zaid M; Department of Medicine, Hospital Sultanah Aminah, Johor, Malaysia.
Wong CY; Department of Otorhinolaryngology, Hospital Umum Sarawak, Sarawak, Malaysia.
Chua HH; Department of Medicine, Hospital Umum Sarawak, Sarawak, Malaysia.
Yusuf S; Department of Otorhinolaryngology, Hospital Tengku Ampuan Afzan, Pahang, Malaysia.
Muhamad D; Department of Medicine, Hospital Tengku Ampuan Afzan, Pahang, Malaysia.
Devesahayam PR; Department of Otorhinolaryngology, Hospital Raja Permaisuri Bainun, Perak, Malaysia.
Ker HB; Department of Medicine, Hospital Raja Permaisuri Bainun, Perak, Malaysia.
Salahuddin Z; Department of Otorhinolaryngology, Hospital Raja Perempuan Zainab II, Kelantan, Malaysia.
Mustafa M; Department of Medicine, Hospital Raja Perempuan Zainab II, Kelantan, Malaysia.
Sawali H; Department of Otorhinolaryngology, Hospital Queen Elizabeth, Sabah, Malaysia.
Lee HG; Department of Medicine, Hospital Queen Elizabeth, Sabah, Malaysia.
Din S; Department of Otorhinolaryngology, Hospital Sungai Buloh, Selangor, Malaysia.
Misnan NA; Department of Medicine, Hospital Sungai Buloh, Selangor, Malaysia.
Mohamad A; Department of Otorhinolaryngology, Hospital Sultanah Nur Zahirah, Terengganu, Malaysia.
Ismail MN; Department of Medicine, Hospital Sultanah Nur Zahirah, Terengganu, Malaysia.
Periasamy C; Department of Otorhinolaryngology, Hospital Pulau Pinang, Penang, Malaysia.
Chow TS; Department of Medicine, Hospital Pulau Pinang, Penang, Malaysia.
Krishnan EK; Department of Otorhinolaryngology, Hospital Kuala Lumpur, Kuala Lumpur, Malaysia.
Leong CL; Department of Medicine, Hospital Kuala Lumpur Hospital, Kuala Lumpur, Malaysia.
Lim LPF; Department of Otorhinolaryngology, Hospital Melaka, Melaka, Malaysia.
Zaidan NZ; Department of Medicine, Hospital Melaka, Melaka, Malaysia.
Ibrahim MZ; Department of Otorhinolaryngology, Hospital Tuanku Fauziah, Perlis, Malaysia.
Abd Wahab S; Department of Medicine, Hospital Tuanku Fauziah, Perlis, Malaysia.
Mohd Hashim SS; Department of Otorhinolaryngology, Hospital Sultanah Bahiyah, KM 6 Jalan Langgar, Alor Setar, 05450, Kedah, Malaysia.
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Corporate Authors:
Malaysian COVID-19 Anosmia Research Group
Źródło:
Scientific reports [Sci Rep] 2022 Feb 08; Vol. 12 (1), pp. 2111. Date of Electronic Publication: 2022 Feb 08.
Typ publikacji:
Clinical Trial; Journal Article; Multicenter Study
MeSH Terms:
COVID-19 Nucleic Acid Testing*
Olfaction Disorders*/diagnosis
Olfaction Disorders*/epidemiology
Olfaction Disorders*/etiology
Olfaction Disorders*/physiopathology
SARS-CoV-2*
Self Report*
Surveys and Questionnaires*
Taste Disorders*/diagnosis
Taste Disorders*/epidemiology
Taste Disorders*/etiology
Taste Disorders*/physiopathology
Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19/complications ; COVID-19/diagnosis ; COVID-19/epidemiology ; COVID-19/physiopathology ; Female ; Humans ; Malaysia/epidemiology ; Male ; Middle Aged
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Development of SARS-CoV-2 packaged RNA reference material for nucleic acid testing.
Autorzy:
Lee SS; Bio-Metrology Group, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon, Korea.
Kim S; Bio-Metrology Group, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon, Korea.; Department of Bio-Analytical Science, University of Science & Technology (UST), Daejeon, 34113, Korea.; Convergent Research Center for Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon, 34114, Korea.
Yoo HM; Bio-Metrology Group, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon, Korea.; Department of Bio-Analytical Science, University of Science & Technology (UST), Daejeon, 34113, Korea.
Lee DH; Bio-Metrology Group, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon, Korea. .
Bae YK; Bio-Metrology Group, Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon, Korea. .; Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Republic of Korea. .
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Źródło:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2022 Feb; Vol. 414 (5), pp. 1773-1785. Date of Electronic Publication: 2021 Dec 27.
Typ publikacji:
Journal Article
MeSH Terms:
Genome, Viral*
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
RNA, Viral/*genetics
Reagent Kits, Diagnostic/*standards
SARS-CoV-2/*genetics
COVID-19/virology ; COVID-19 Nucleic Acid Testing/standards ; Coronavirus Envelope Proteins/genetics ; Coronavirus Envelope Proteins/metabolism ; Coronavirus Nucleocapsid Proteins/genetics ; Coronavirus Nucleocapsid Proteins/metabolism ; Coronavirus RNA-Dependent RNA Polymerase/genetics ; Coronavirus RNA-Dependent RNA Polymerase/metabolism ; Gene Dosage ; Gene Expression ; Humans ; Jurkat Cells ; Lentivirus/genetics ; Lentivirus/metabolism ; Phosphoproteins/genetics ; Phosphoproteins/metabolism ; RNA, Viral/metabolism ; RNA, Viral/standards ; Reagent Kits, Diagnostic/supply & distribution ; Reference Standards ; Reproducibility of Results ; Spike Glycoprotein, Coronavirus/genetics ; Spike Glycoprotein, Coronavirus/metabolism ; Viral Genome Packaging
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Estimate false-negative RT-PCR rates for SARS-CoV-2. A systematic review and meta-analysis.
Autorzy:
Pecoraro V; Department of Laboratory Medicine and Pathology, Azienda USL of Modena, Modena, Italy.
Negro A; Health and Social Regional Agency of Emilia-Romagna Region, Bologna, Italy.
Pirotti T; Department of Laboratory Medicine and Pathology, Azienda USL of Modena, Modena, Italy.
Trenti T; Department of Laboratory Medicine and Pathology, Azienda USL of Modena, Modena, Italy.
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Źródło:
European journal of clinical investigation [Eur J Clin Invest] 2022 Feb; Vol. 52 (2), pp. e13706. Date of Electronic Publication: 2021 Dec 05.
Typ publikacji:
Journal Article; Meta-Analysis; Systematic Review
MeSH Terms:
COVID-19 Nucleic Acid Testing*
COVID-19/*diagnosis
SARS-CoV-2/*genetics
Diagnostic Errors ; False Negative Reactions ; Humans ; RNA, Viral
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Pooling saliva samples as an excellent option to increase the surveillance for SARS-CoV-2 when re-opening community settings.
Autorzy:
Moreno-Contreras J; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología UNAM, Cuernavaca, Morelos, México.
Espinoza MA; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología UNAM, Cuernavaca, Morelos, México.
Sandoval-Jaime C; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología UNAM, Cuernavaca, Morelos, México.
Cantú-Cuevas MA; Secretaría de Salud del Edo. de Morelos, Cuernavaca, Morelos, México.
Madrid-González DA; Secretaría de Salud del Edo. de Morelos, Cuernavaca, Morelos, México.
Barón-Olivares H; Servicios de Salud del Edo. de Morelos, Cuernavaca, Morelos, México.
Ortiz-Orozco OD; Servicios de Salud del Edo. de Morelos, Cuernavaca, Morelos, México.
Muñoz-Rangel AV; Servicios de Salud del Edo. de Morelos, Cuernavaca, Morelos, México.
Guzmán-Rodríguez C; Servicios de Salud del Edo. de Morelos, Cuernavaca, Morelos, México.
Hernández-de la Cruz M; Servicios de Salud del Edo. de Morelos, Cuernavaca, Morelos, México.
Eroza-Osorio CM; Servicios de Salud del Edo. de Morelos, Cuernavaca, Morelos, México.
Arias CF; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología UNAM, Cuernavaca, Morelos, México.
López S; Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología UNAM, Cuernavaca, Morelos, México.
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Źródło:
PloS one [PLoS One] 2022 Jan 25; Vol. 17 (1), pp. e0263114. Date of Electronic Publication: 2022 Jan 25 (Print Publication: 2022).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
Saliva/*virology
COVID-19/epidemiology ; COVID-19/prevention & control ; COVID-19 Nucleic Acid Testing/standards ; Humans ; Mass Screening/methods ; Mass Screening/standards ; Quarantine/standards ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/pathogenicity ; Sensitivity and Specificity
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
The application of a deep learning system developed to reduce the time for RT-PCR in COVID-19 detection.
Autorzy:
Lee Y; Department of Emergency Medicine, College of Medicine, Hallym University, Chuncheon, South Korea.; Hallym Bioinformatics & Convergence Research Laboratory, Hallym Translation Research Center, Kangnam Sacred-Heart Hospital, Hallym University, Chuncheon, South Korea.
Kim YS; Department of Convergence Software, Hallym University, Chuncheon, South Korea.
Lee DI; Department of Convergence Software, Hallym University, Chuncheon, South Korea.
Jeong S; Department of Laboratory Medicine, Kangnam Sacred Heart Hospital, Hallym University College of Medicine, 6, Singil-ro, 1-gil, Youngdeungpo-gu, Seoul, 07441, South Korea. .
Kang GH; Department of Emergency Medicine, College of Medicine, Hallym University, Chuncheon, South Korea.; Hallym Bioinformatics & Convergence Research Laboratory, Hallym Translation Research Center, Kangnam Sacred-Heart Hospital, Hallym University, Chuncheon, South Korea.
Jang YS; Department of Emergency Medicine, College of Medicine, Hallym University, Chuncheon, South Korea.; Hallym Bioinformatics & Convergence Research Laboratory, Hallym Translation Research Center, Kangnam Sacred-Heart Hospital, Hallym University, Chuncheon, South Korea.
Kim W; Department of Emergency Medicine, College of Medicine, Hallym University, Chuncheon, South Korea.; Hallym Bioinformatics & Convergence Research Laboratory, Hallym Translation Research Center, Kangnam Sacred-Heart Hospital, Hallym University, Chuncheon, South Korea.
Choi HY; Department of Emergency Medicine, College of Medicine, Hallym University, Chuncheon, South Korea.; Hallym Bioinformatics & Convergence Research Laboratory, Hallym Translation Research Center, Kangnam Sacred-Heart Hospital, Hallym University, Chuncheon, South Korea.
Kim JG; Department of Emergency Medicine, College of Medicine, Hallym University, Chuncheon, South Korea.; Hallym Bioinformatics & Convergence Research Laboratory, Hallym Translation Research Center, Kangnam Sacred-Heart Hospital, Hallym University, Chuncheon, South Korea.
Choi SH; Hallym Bioinformatics & Convergence Research Laboratory, Hallym Translation Research Center, Kangnam Sacred-Heart Hospital, Hallym University, Chuncheon, South Korea.; Department of Biomedical Engineering, Yonsei University, Wonju, South Korea.
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Źródło:
Scientific reports [Sci Rep] 2022 Jan 24; Vol. 12 (1), pp. 1234. Date of Electronic Publication: 2022 Jan 24.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19 Nucleic Acid Testing*
Deep Learning*
Reverse Transcriptase Polymerase Chain Reaction*
SARS-CoV-2/*genetics
Humans ; Sensitivity and Specificity
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Distinct Outcomes in COVID-19 Patients with Positive or Negative RT-PCR Test.
Autorzy:
Saad Menezes MC; Emergency Medicine Department, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil.
Santinelli Pestana DV; Emergency Medicine Department, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil.
Ferreira JC; Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903l, Brazil.
Ribeiro de Carvalho CR; Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903l, Brazil.
Felix MC; Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903l, Brazil.
Marcilio IO; Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903l, Brazil.
da Silva KR; Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903l, Brazil.
Junior VC; Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903l, Brazil.
Marchini JF; Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903l, Brazil.
Alencar JC; Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903l, Brazil.
Gomez LMG; Emergency Medicine Department, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil.
Mauá DD; Institute of Mathematics and Statistics, Universidade de São Paulo, São Paulo 05508-090, Brazil.
Souza HP; Emergency Medicine Department, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil.
Emergency Usp Covid-Group; Emergency Medicine Department, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903, Brazil.
Hcfmusp Covid-Study Group; Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo 01246-903l, Brazil.
Pokaż więcej
Źródło:
Viruses [Viruses] 2022 Jan 18; Vol. 14 (2). Date of Electronic Publication: 2022 Jan 18.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*statistics & numerical data
Reverse Transcriptase Polymerase Chain Reaction/*statistics & numerical data
SARS-CoV-2/*genetics
Academic Medical Centers/statistics & numerical data ; Aged ; Brazil ; COVID-19/mortality ; COVID-19/virology ; COVID-19 Nucleic Acid Testing/methods ; Female ; Hospital Mortality ; Hospitalization/statistics & numerical data ; Humans ; Intensive Care Units/statistics & numerical data ; Male ; Middle Aged ; Nasopharynx/virology ; Retrospective Studies ; Risk Factors
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Nucleocapsid (N) Gene Mutations of SARS-CoV-2 Can Affect Real-Time RT-PCR Diagnostic and Impact False-Negative Results.
Autorzy:
Lesbon JCC; Laboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, São Paulo, Brazil.
Poleti MD; Laboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, São Paulo, Brazil.
de Mattos Oliveira EC; Laboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, São Paulo, Brazil.
Patané JSL; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Clemente LG; Functional Genomic Center, Department of Animal Science, Luiz de Queiroz School of Agriculture, University of Sao Paulo, Piracicaba 13418-900, São Paulo, Brazil.
Viala VL; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Ribeiro G; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Giovanetti M; Fundação Oswaldo Cruz, FIOCRUZ, Manguinhos 21040-900, Rio de Janeiro, Brazil.; Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, Minas Gerais, Brazil.
de Alcantara LCJ; Fundação Oswaldo Cruz, FIOCRUZ, Manguinhos 21040-900, Rio de Janeiro, Brazil.
Teixeira O; Ribeirao Preto Protein Crystallography Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Ribeirao Preto 14040-903, São Paulo, Brazil
Nonato MC; Ribeirao Preto Protein Crystallography Laboratory, School of Pharmaceutical Sciences, University of São Paulo, Ribeirao Preto 14040-903, São Paulo, Brazil
de Lima LPO; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Martins AJ; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Dos Santos Barros CR; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Marqueze EC; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
de Souza Todão Bernardino J; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Moretti DB; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Brassaloti RA; Functional Genomic Center, Department of Animal Science, Luiz de Queiroz School of Agriculture, University of Sao Paulo, Piracicaba 13418-900, São Paulo, Brazil.
de Lello Rocha Campos Cassano R; Functional Genomic Center, Department of Animal Science, Luiz de Queiroz School of Agriculture, University of Sao Paulo, Piracicaba 13418-900, São Paulo, Brazil.
Mariani PDSC; NGS Soluções Genômicas, Piracicaba 13416-030, São Paulo, Brazil.
Slavov SN; Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14051-060, São Paulo, Brazil
Dos Santos RB; Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14051-060, São Paulo, Brazil
Rodrigues ES; Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14051-060, São Paulo, Brazil
Santos EV; Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14051-060, São Paulo, Brazil
Borges JS; Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14051-060, São Paulo, Brazil
de La Roque DGL; Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14051-060, São Paulo, Brazil
Kitajima JP; Mendelics Genomic Analysis, São Paulo 02511-000, São Paulo, Brazil.
Santos B; Mendelics Genomic Analysis, São Paulo 02511-000, São Paulo, Brazil.
Assato PA; School of Agricultural Sciences, São Paulo State University, Botucatu 18618-970, São Paulo, Brazil.
da Silva da Costa FA; School of Agricultural Sciences, São Paulo State University, Botucatu 18618-970, São Paulo, Brazil.
Banho CA; Laboratório de Pesquisas em Virologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto 15090-000, São Paulo, Brazil.
Sacchetto L; Laboratório de Pesquisas em Virologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto 15090-000, São Paulo, Brazil.
Moraes MM; Laboratório de Pesquisas em Virologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto 15090-000, São Paulo, Brazil.
Palmieri M; Coordenação de Vigilância em Saúde-Secretaria Municipal da Saúde, São Paulo, São Paulo 01223-906, Brazil.
da Silva FEV; Laboratory Assistance, Coordination of Primary Care, Municipal Health Department, São Paulo, São Paulo 01223-906, Brazil.
Grotto RMT; School of Agricultural Sciences, São Paulo State University, Botucatu 18618-970, São Paulo, Brazil.
Souza-Neto JA; School of Agricultural Sciences, São Paulo State University, Botucatu 18618-970, São Paulo, Brazil.
Nogueira ML; Laboratório de Pesquisas em Virologia, Departamento de Doenças Dermatológicas, Infecciosas e Parasitárias, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto 15090-000, São Paulo, Brazil.
Coutinho LL; Functional Genomic Center, Department of Animal Science, Luiz de Queiroz School of Agriculture, University of Sao Paulo, Piracicaba 13418-900, São Paulo, Brazil.
Calado RT; Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14051-060, São Paulo, Brazil
Neto RM; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Covas DT; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.; Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14051-060, São Paulo, Brazil
Kashima S; Blood Center of Ribeirão Preto, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto 14051-060, São Paulo, Brazil
Elias MC; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Sampaio SC; Butantan Institute, São Paulo 05503-000, São Paulo, Brazil.
Fukumasu H; Laboratory of Comparative and Translational Oncology, Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of Sao Paulo, Pirassununga 13635-900, São Paulo, Brazil.
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Źródło:
Viruses [Viruses] 2021 Dec 10; Vol. 13 (12). Date of Electronic Publication: 2021 Dec 10.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19 Nucleic Acid Testing*
COVID-19/*diagnosis
Coronavirus Nucleocapsid Proteins/*genetics
SARS-CoV-2/*isolation & purification
Brazil/epidemiology ; COVID-19/epidemiology ; Coronavirus RNA-Dependent RNA Polymerase/genetics ; DNA Primers ; False Negative Reactions ; Genome, Viral/genetics ; Humans ; Mutation ; Phosphoproteins/genetics ; RNA, Viral/genetics ; SARS-CoV-2/genetics
SCR Organism:
SARS-CoV-2 variants
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting.
Autorzy:
Ferreira BIDS; Real Time PCR Platform RPT09A, Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
da Silva-Gomes NL; Real Time PCR Platform RPT09A, Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Coelho WLDCNP; Laboratory of Technological Development in Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
da Costa VD; Laboratory of Viral Hepatitis, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Carneiro VCS; Laboratory of Molecular Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Kader RL; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Amaro MP; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Villar LM; Laboratory of Viral Hepatitis, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Miyajima F; Oswaldo Cruz Foundation (Fiocruz), Branch Ceará, Eusebio, Brazil.
Alves-Leon SV; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
de Paula VS; Laboratory of Molecular Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Leon LAA; Laboratory of Technological Development in Virology, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
Moreira OC; Real Time PCR Platform RPT09A, Laboratory of Molecular Biology and Endemic Diseases, Oswaldo Cruz Institute/ Fiocruz, Rio de Janeiro, Brazil.
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Źródło:
PloS one [PLoS One] 2021 Nov 22; Vol. 16 (11), pp. e0260087. Date of Electronic Publication: 2021 Nov 22 (Print Publication: 2021).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms:
COVID-19 Nucleic Acid Testing/*methods
Real-Time Polymerase Chain Reaction/*methods
Adult ; COVID-19 Nucleic Acid Testing/standards ; Female ; Humans ; Male ; Nucleic Acid Denaturation ; Oligonucleotides/chemistry ; Real-Time Polymerase Chain Reaction/standards ; Respiratory Mucosa/virology ; SARS-CoV-2/genetics ; SARS-CoV-2/pathogenicity ; Saliva/virology ; Sensitivity and Specificity
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Comparative analysis of loop-mediated isothermal amplification (LAMP)-based assays for rapid detection of SARS-CoV-2 genes.
Autorzy:
Urrutia-Cabrera D; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Australia.; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia.
Liou RH; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Australia.; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia.
Wang JH; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Australia.; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia.
Chan J; Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Australia.; Departments of Medicine and Neurology, University of Melbourne, Royal Melbourne Hospital, Melbourne, Australia.
Hung SS; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Australia.; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia.
Hewitt AW; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Australia.; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia.
Martin KR; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Australia.; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia.
Edwards TL; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Australia.; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia.
Kwan P; Department of Neuroscience, Central Clinical School, Monash University, Melbourne, Australia.; Departments of Medicine and Neurology, University of Melbourne, Royal Melbourne Hospital, Melbourne, Australia.
Wong RC; Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital, Melbourne, Australia. .; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia. .; Shenzhen Eye Hospital, Shenzhen University School of Medicine, Shenzhen, China. .
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Źródło:
Scientific reports [Sci Rep] 2021 Nov 18; Vol. 11 (1), pp. 22493. Date of Electronic Publication: 2021 Nov 18.
Typ publikacji:
Clinical Trial; Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19*/metabolism
COVID-19 Nucleic Acid Testing*
Molecular Diagnostic Techniques*
Nucleic Acid Amplification Techniques*
SARS-CoV-2/*genetics
Saliva/*metabolism
Adult ; Female ; Humans ; Male ; Saliva/virology
SCR Protocol:
LAMP assay
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
High sensitivity-low cost detection of SARS-CoV-2 by two steps end point RT-PCR with agarose gel electrophoresis visualization.
Autorzy:
Figueroa S; One Health Research Group, Universidad de Las Americas, Quito, Ecuador.
Freire-Paspuel B; One Health Research Group, Universidad de Las Americas, Quito, Ecuador.
Vega-Mariño P; Agencia de Regulación y Control de la Bioseguridad y Cuarentena para Galápagos, Puerto Ayora, Ecuador.
Velez A; Agencia de Regulación y Control de la Bioseguridad y Cuarentena para Galápagos, Puerto Ayora, Ecuador.
Cruz M; Agencia de Regulación y Control de la Bioseguridad y Cuarentena para Galápagos, Puerto Ayora, Ecuador.
Cardenas WB; Laboratorio para Investigaciones Biomédicas, Escuela Superior Politécnica del Litoral, Guayaquil, Ecuador.
Garcia-Bereguiain MA; One Health Research Group, Universidad de Las Americas, Quito, Ecuador. .
Pokaż więcej
Źródło:
Scientific reports [Sci Rep] 2021 Nov 04; Vol. 11 (1), pp. 21658. Date of Electronic Publication: 2021 Nov 04.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
SARS-CoV-2/*genetics
COVID-19/genetics ; COVID-19 Nucleic Acid Testing/economics ; COVID-19 Testing/methods ; Coronavirus Nucleocapsid Proteins/genetics ; DNA Primers ; Electrophoresis, Agar Gel/methods ; Humans ; Laboratories ; Nasopharynx/virology ; RNA, Viral/genetics ; Ribonuclease P/genetics ; Ribonuclease P/metabolism ; SARS-CoV-2/pathogenicity ; Sensitivity and Specificity
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Optimizing and evaluating PCR-based pooled screening during COVID-19 pandemics.
Autorzy:
Yu J; Tsinghua-Berkeley Shenzhen Institute (TBSI), Tsinghua University, Shenzhen, China.
Huang Y; Department of Civil and Environmental Engineering, University of California Berkeley, Berkeley, CA, USA.
Shen ZJ; College of Engineering, University of California Berkeley, Berkeley, CA, USA. .; Faculty of Engineering and Faculty of Business and Economics, University of Hong Kong, Hong Kong, China. .
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Źródło:
Scientific reports [Sci Rep] 2021 Nov 02; Vol. 11 (1), pp. 21460. Date of Electronic Publication: 2021 Nov 02.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
SARS-CoV-2/*genetics
Antigens, Viral/genetics ; Antigens, Viral/metabolism ; COVID-19/epidemiology ; COVID-19/virology ; COVID-19 Nucleic Acid Testing/economics ; False Negative Reactions ; False Positive Reactions ; Humans ; Pandemics ; Polymerase Chain Reaction/economics ; Reproducibility of Results ; SARS-CoV-2/isolation & purification ; Viral Load
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
SARS-CoV-2 RNA may rarely be present in a uterine cervix LBC sample at the asymptomatic early stage of COVID 19 disease.
Autorzy:
Ondič O; Department of Pathology, Medical Faculty, Charles University and Charles University Hospital Pilsen, Pilsen, Czech Republic.; Bioptická Laboratoř, s.r.o., Pilsen, Czech Republic.
Černá K; Department of Pathology, Medical Faculty, Charles University and Charles University Hospital Pilsen, Pilsen, Czech Republic.; Bioptická Laboratoř, s.r.o., Pilsen, Czech Republic.
Kinkorová-Luňáčková I; Bioptická Laboratoř, s.r.o., Pilsen, Czech Republic.
Němcová J; Department of Pathology, Medical Faculty, Charles University and Charles University Hospital Pilsen, Pilsen, Czech Republic.; Bioptická Laboratoř, s.r.o., Pilsen, Czech Republic.
Mejchar B; Private Gynaecologist Office, Pilsen, Czech Republic.
Chytra J; Department of Gynecology and Obstetrics, Medical Faculty, Charles University and Charles University Hospital Pilsen, Pilsen, Czech Republic.
Bouda J; Department of Gynecology and Obstetrics, Medical Faculty, Charles University and Charles University Hospital Pilsen, Pilsen, Czech Republic.
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Źródło:
Cytopathology : official journal of the British Society for Clinical Cytology [Cytopathology] 2021 Nov; Vol. 32 (6), pp. 766-770. Date of Electronic Publication: 2021 May 31.
Typ publikacji:
Journal Article
MeSH Terms:
COVID-19*/diagnosis
COVID-19*/genetics
COVID-19*/metabolism
COVID-19 Nucleic Acid Testing*
Papanicolaou Test*
SARS-CoV-2*/genetics
SARS-CoV-2*/metabolism
Vaginal Smears*
Adult ; Female ; Humans ; Middle Aged ; Retrospective Studies ; Uterine Cervical Neoplasms/diagnosis ; Uterine Cervical Neoplasms/genetics ; Uterine Cervical Neoplasms/metabolism ; Uterine Cervical Neoplasms/virology
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Predictive usefulness of RT-PCR testing in different patterns of Covid-19 symptomatology: analysis of a French cohort of 12,810 outpatients.
Corporate Authors:
AP-HP/Universities/Inserm COVID-19 Research Collaboration
Źródło:
Scientific reports [Sci Rep] 2021 Oct 27; Vol. 11 (1), pp. 21233. Date of Electronic Publication: 2021 Oct 27.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
SARS-CoV-2*
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
Adolescent ; Adult ; Aged ; Aged, 80 and over ; COVID-19/diagnostic imaging ; COVID-19/epidemiology ; COVID-19 Nucleic Acid Testing/statistics & numerical data ; Cohort Studies ; Female ; France/epidemiology ; Humans ; Male ; Middle Aged ; Pandemics ; Predictive Value of Tests ; Retrospective Studies ; Telemedicine/methods ; Telemedicine/statistics & numerical data ; Young Adult
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
SARS-CoV-2 infection in central North Carolina: Protocol for a population-based longitudinal cohort study and preliminary participant results.
Autorzy:
Miller EM; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Law EA; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Ajeen R; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Karasik J; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Mendoza C; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Abernathy H; Institute of Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Garrett H; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
King E; Institute of Global Health and Infectious Diseases, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Wallace J; North Carolina Institute of Public Health, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Zelek M; Chatham County Public Health Department, Pittsboro, NC, United States of America.
Edwards JK; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Xiong K; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Beatty C; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Fleischauer AT; Division of State and Local Readiness, United States Centers for Disease Control and Prevention, Atlanta, GA, United States of America.; Epidemiology Branch, North Carolina Department of Health and Human Services, Raleigh, NC, United States of America.
Ciccone EJ; Division of Infectious Diseases, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Shook-Sa BE; Department of Biostatistics, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Aiello AE; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.; Carolina Population Center, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
Boyce RM; Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.; Division of Infectious Diseases, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.
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Źródło:
PloS one [PLoS One] 2021 Oct 25; Vol. 16 (10), pp. e0259070. Date of Electronic Publication: 2021 Oct 25 (Print Publication: 2021).
Typ publikacji:
Journal Article; Observational Study; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
MeSH Terms:
Population Surveillance*
COVID-19/*epidemiology
COVID-19 Nucleic Acid Testing/*statistics & numerical data
COVID-19 Serological Testing/*statistics & numerical data
Adult ; COVID-19/diagnosis ; COVID-19 Nucleic Acid Testing/methods ; COVID-19 Serological Testing/methods ; Cohort Studies ; Demography/statistics & numerical data ; Female ; Humans ; Male ; North Carolina ; Practice Guidelines as Topic ; Risk
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
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