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Wyszukujesz frazę ""CRISPR-Cas Systems"" wg kryterium: Temat


Tytuł:
Efficient, specific, and combinatorial control of endogenous exon splicing with dCasRx-RBM25.
Autorzy:
Li JD; Donnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Taipale M; Donnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada. Electronic address: .
Blencowe BJ; Donnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada. Electronic address: .
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Źródło:
Molecular cell [Mol Cell] 2024 Jul 11; Vol. 84 (13), pp. 2573-2589.e5. Date of Electronic Publication: 2024 Jun 24.
Typ publikacji:
Journal Article
MeSH Terms:
Exons*
CRISPR-Cas Systems*
RNA-Binding Proteins*/genetics
RNA-Binding Proteins*/metabolism
Alternative Splicing*
RNA Splicing Factors*/genetics
RNA Splicing Factors*/metabolism
Humans ; HEK293 Cells ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Animals ; Mice
Czasopismo naukowe
Tytuł:
ABE-ultramax for high-efficiency biallelic adenine base editing in zebrafish.
Autorzy:
Qin W; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Liang F; Institute of Modern Aquaculture Science and Engineering, School of Life Sciences, South China Normal University, Guangzhou, Guangdong, 510631, China.
Lin SJ; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Petree C; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Huang K; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Zhang Y; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Li L; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, South China Normal University, 510631, Guangzhou, China.; Institute for Brain Research and Rehabilitation, and Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, 510631, Guangzhou, China.
Varshney P; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
Mourrain P; Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.
Liu Y; Key Laboratory of Brain, Cognition and Education Sciences, Ministry of Education, South China Normal University, 510631, Guangzhou, China. .; Institute for Brain Research and Rehabilitation, and Guangdong Key Laboratory of Mental Health and Cognitive Science, South China Normal University, 510631, Guangzhou, China. .
Varshney GK; Genes & Human Disease Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA. .
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Źródło:
Nature communications [Nat Commun] 2024 Jul 04; Vol. 15 (1), pp. 5613. Date of Electronic Publication: 2024 Jul 04.
Typ publikacji:
Journal Article
MeSH Terms:
Zebrafish*/genetics
Gene Editing*/methods
Adenine*/metabolism
CRISPR-Cas Systems*
INDEL Mutation*
Animals ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Animals, Genetically Modified ; Alleles
Czasopismo naukowe
Tytuł:
Transposase-assisted target-site integration for efficient plant genome engineering.
Autorzy:
Liu P; Donald Danforth Plant Science Center, St Louis, MO, USA.
Panda K; Donald Danforth Plant Science Center, St Louis, MO, USA.
Edwards SA; Donald Danforth Plant Science Center, St Louis, MO, USA.; Division of Biological Sciences, University of Missouri, Columbia, MO, USA.
Swanson R; Donald Danforth Plant Science Center, St Louis, MO, USA.; Division of Biological Sciences, University of Missouri, Columbia, MO, USA.
Yi H; Plant Transformation Facility, Donald Danforth Plant Science Center, St Louis, MO, USA.
Pandesha P; Donald Danforth Plant Science Center, St Louis, MO, USA.; Division of Biology and Biomedical Sciences, Washington University, St Louis, MO, USA.
Hung YH; Donald Danforth Plant Science Center, St Louis, MO, USA.
Klaas G; Donald Danforth Plant Science Center, St Louis, MO, USA.
Ye X; Bayer Crop Science, St Louis, MO, USA.
Collins MV; University of South Carolina-Aiken, Aiken, SC, USA.
Renken KN; University of South Carolina-Aiken, Aiken, SC, USA.
Gilbertson LA; Bayer Crop Science, St Louis, MO, USA.
Veena V; Plant Transformation Facility, Donald Danforth Plant Science Center, St Louis, MO, USA.
Hancock CN; University of South Carolina-Aiken, Aiken, SC, USA.
Slotkin RK; Donald Danforth Plant Science Center, St Louis, MO, USA. .; Division of Biological Sciences, University of Missouri, Columbia, MO, USA. .
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Źródło:
Nature [Nature] 2024 Jul; Vol. 631 (8021), pp. 593-600. Date of Electronic Publication: 2024 Jun 26.
Typ publikacji:
Journal Article
MeSH Terms:
Transposases*/metabolism
Transposases*/genetics
Arabidopsis*/genetics
Oryza*/genetics
Genome, Plant*/genetics
DNA Transposable Elements*/genetics
Gene Editing*/methods
CRISPR-Cas Systems*/genetics
Open Reading Frames/genetics ; Enhancer Elements, Genetic/genetics ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Mutagenesis, Insertional/genetics ; CRISPR-Associated Proteins/metabolism ; CRISPR-Associated Proteins/genetics ; CRISPR-Associated Protein 9/metabolism ; CRISPR-Associated Protein 9/genetics ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Genetic Engineering/methods ; Plants, Genetically Modified/genetics ; Endodeoxyribonucleases
Czasopismo naukowe
Tytuł:
TnpB homologues exapted from transposons are RNA-guided transcription factors.
Autorzy:
Wiegand T; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Hoffmann FT; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Walker MWG; Department of Biological Sciences, Columbia University, New York, NY, USA.
Tang S; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Richard E; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Le HC; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Meers C; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.
Sternberg SH; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA. .
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Źródło:
Nature [Nature] 2024 Jul; Vol. 631 (8020), pp. 439-448. Date of Electronic Publication: 2024 Jun 26.
Typ publikacji:
Journal Article
MeSH Terms:
DNA Transposable Elements*/genetics
Enterobacteriaceae*/genetics
Enterobacteriaceae*/virology
Evolution, Molecular*
RNA, Guide, CRISPR-Cas Systems*/genetics
RNA, Guide, CRISPR-Cas Systems*/metabolism
Transcription Factors*/metabolism
Transcription Factors*/genetics
Transposases*/metabolism
Transposases*/genetics
Bacteriophages/genetics ; CRISPR-Associated Protein 9 ; CRISPR-Cas Systems/genetics ; Escherichia coli/genetics ; Escherichia coli/virology ; Phylogeny ; Promoter Regions, Genetic/genetics ; Repetitive Sequences, Nucleic Acid ; Repressor Proteins/metabolism ; Repressor Proteins/genetics ; Enterobacter/genetics ; Enterobacter/virology
Czasopismo naukowe
Tytuł:
PAM-flexible Engineered FnCas9 variants for robust and ultra-precise genome editing and diagnostics.
Autorzy:
Acharya S; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India. .; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India. .
Ansari AH; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.
Kumar Das P; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.
Hirano S; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Aich M; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.
Rauthan R; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.
Mahato S; Center for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, LV Prasad Eye Institute, Hyderabad, 500034, Telangana, India.; Manipal Academy of Higher Education, Manipal, Karnataka, India.
Maddileti S; Center for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, LV Prasad Eye Institute, Hyderabad, 500034, Telangana, India.
Sarkar S; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.
Kumar M; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.
Phutela R; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.
Gulati S; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.
Rahman A; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.
Goel A; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.
Afzal C; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.
Paul D; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.
Agrawal T; Center for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, LV Prasad Eye Institute, Hyderabad, 500034, Telangana, India.; Manipal Academy of Higher Education, Manipal, Karnataka, India.
Pulimamidi VK; Center for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, LV Prasad Eye Institute, Hyderabad, 500034, Telangana, India.; Schepens Eye Research Institute, Massachusetts Eye and Ear, Harvard Medical School, Boston, MA, 02114, USA.
Jalali S; Srimati Kannuri Santhamma Centre for vitreoretinal diseases, Anant Bajaj Retina Institute, Kallam Anji Reddy Campus, L V Prasad Eye Institute, Hyderabad, Telangana, India.
Nishimasu H; Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8656, Japan.; Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8904, Japan.; Inamori Research Institute for Science, 620 Suiginya-cho, Shimogyo-ku, Kyoto, 600-8411, Japan.
Mariappan I; Center for Ocular Regeneration, Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, LV Prasad Eye Institute, Hyderabad, 500034, Telangana, India.
Nureki O; Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
Maiti S; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India.; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.
Chakraborty D; CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India. .; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India. .
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Źródło:
Nature communications [Nat Commun] 2024 Jun 28; Vol. 15 (1), pp. 5471. Date of Electronic Publication: 2024 Jun 28.
Typ publikacji:
Journal Article
MeSH Terms:
Gene Editing*/methods
CRISPR-Associated Protein 9*/metabolism
CRISPR-Associated Protein 9*/genetics
CRISPR-Cas Systems*/genetics
Francisella*/genetics
Humans ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Leber Congenital Amaurosis/genetics ; Streptococcus pyogenes/genetics ; HEK293 Cells ; Mutation ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Protein Engineering/methods ; Genome, Human
SCR Organism:
Francisella tularensis subsp. novicida
Czasopismo naukowe
Tytuł:
Identification of functional sgRNA mutants lacking canonical secondary structure using high-throughput FACS screening.
Autorzy:
Liang Z; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Huang C; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Xia Y; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Ye Z; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Fan S; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Zeng J; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Guo S; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Ma X; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China; Beijing Institute of Technology (Tangshan) Translational Research Center, Hebei 063611, China.
Sun L; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China; Beijing Institute of Technology (Tangshan) Translational Research Center, Hebei 063611, China.
Huo YX; Key Laboratory of Molecular Medicine and Biotherapy, Aerospace Center Hospital, School of Life Science, Beijing Institute of Technology, Beijing 100081, China; Beijing Institute of Technology (Tangshan) Translational Research Center, Hebei 063611, China. Electronic address: .
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Źródło:
Cell reports [Cell Rep] 2024 Jun 25; Vol. 43 (6), pp. 114290. Date of Electronic Publication: 2024 May 31.
Typ publikacji:
Journal Article
MeSH Terms:
RNA, Guide, CRISPR-Cas Systems*/metabolism
RNA, Guide, CRISPR-Cas Systems*/genetics
Flow Cytometry*/methods
CRISPR-Cas Systems/genetics ; Mutation/genetics ; Nucleic Acid Conformation ; High-Throughput Screening Assays/methods ; Butanols/metabolism ; Gene Editing/methods ; CRISPR-Associated Protein 9/metabolism ; CRISPR-Associated Protein 9/genetics
Czasopismo naukowe
Tytuł:
Conformational landscape of the type V-K CRISPR-associated transposon integration assembly.
Autorzy:
Tenjo-Castaño F; Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Sofos N; Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Stutzke LS; Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Temperini P; Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Fuglsang A; Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Pape T; Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark; Core Facility for Integrated Microscopy (CFIM), Faculty of Health and Medical Sciences University of Copenhagen; Blegdamsvej 3B, DK-2200 Copenhagen, Denmark.
Mesa P; Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark.
Montoya G; Structural Molecular Biology Group, Novo Nordisk Foundation Centre for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen, Denmark. Electronic address: .
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Źródło:
Molecular cell [Mol Cell] 2024 Jun 20; Vol. 84 (12), pp. 2353-2367.e5. Date of Electronic Publication: 2024 Jun 03.
Typ publikacji:
Journal Article
MeSH Terms:
Cryoelectron Microscopy*
DNA Transposable Elements*/genetics
CRISPR-Cas Systems*
Binding Sites ; Gene Editing/methods ; Models, Molecular ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Clustered Regularly Interspaced Short Palindromic Repeats ; Protein Conformation ; Nucleic Acid Conformation
Czasopismo naukowe
Tytuł:
Streptococcus pyogenes Cas9 ribonucleoprotein delivery for efficient, rapid and marker-free gene editing in Trypanosoma and Leishmania.
Autorzy:
Asencio C; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Hervé P; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Morand P; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Oliveres Q; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Morel CA; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Prouzet-Mauleon V; Univ. Bordeaux, CNRS/INSERM, TBMCore/CRISP'edit, UAR 3427/US005, Bordeaux, France.
Biran M; Univ. Bordeaux, CNRS, CRMSB, UMR 5536, Bordeaux, France.
Monic S; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Bonhivers M; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Robinson DR; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Ouellette M; Centre de Recherche en Infectiologie du Centre de Recherche du CHU de Québec, Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Québec, Canada.
Rivière L; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Bringaud F; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
Tetaud E; Univ. Bordeaux, CNRS, MFP, UMR 5234, Bordeaux, France.
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Źródło:
Molecular microbiology [Mol Microbiol] 2024 Jun; Vol. 121 (6), pp. 1079-1094. Date of Electronic Publication: 2024 Apr 01.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Gene Editing*/methods
CRISPR-Cas Systems*
Ribonucleoproteins*/metabolism
Ribonucleoproteins*/genetics
Streptococcus pyogenes/genetics ; Streptococcus pyogenes/metabolism ; Leishmania/genetics ; Leishmania/metabolism ; CRISPR-Associated Protein 9/genetics ; CRISPR-Associated Protein 9/metabolism ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Trypanosoma brucei brucei/genetics ; Trypanosoma brucei brucei/metabolism ; Trypanosoma/genetics ; Trypanosoma/metabolism ; Transfection
Czasopismo naukowe
Tytuł:
Unity among the diverse RNA-guided CRISPR-Cas interference mechanisms.
Autorzy:
Ganguly C; Department of Chemistry and Biochemistry, Price Family Foundation Institute of Structural Biology, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma, USA.
Rostami S; Department of Chemistry and Biochemistry, Price Family Foundation Institute of Structural Biology, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma, USA.
Long K; Department of Chemistry and Biochemistry, Price Family Foundation Institute of Structural Biology, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma, USA.
Aribam SD; Department of Chemistry and Biochemistry, Price Family Foundation Institute of Structural Biology, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma, USA.
Rajan R; Department of Chemistry and Biochemistry, Price Family Foundation Institute of Structural Biology, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, Oklahoma, USA. Electronic address: .
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Źródło:
The Journal of biological chemistry [J Biol Chem] 2024 Jun; Vol. 300 (6), pp. 107295. Date of Electronic Publication: 2024 Apr 18.
Typ publikacji:
Journal Article; Review
MeSH Terms:
CRISPR-Cas Systems*
RNA, Guide, CRISPR-Cas Systems/metabolism ; RNA, Guide, CRISPR-Cas Systems/genetics ; Archaea/genetics ; Archaea/metabolism ; Bacteria/genetics ; Bacteria/metabolism ; CRISPR-Associated Proteins/metabolism ; CRISPR-Associated Proteins/genetics ; CRISPR-Associated Proteins/chemistry ; Clustered Regularly Interspaced Short Palindromic Repeats
Czasopismo naukowe
Tytuł:
Live-cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a.
Autorzy:
Olivi L; Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.
Bagchus C; Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.; Laboratory of Biophysics, Wageningen University & Research, Wageningen, The Netherlands.
Pool V; Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.; Laboratory of Biophysics, Wageningen University & Research, Wageningen, The Netherlands.
Bekkering E; Laboratory of Biophysics, Wageningen University & Research, Wageningen, The Netherlands.
Speckner K; Laboratory of Biophysics, Wageningen University & Research, Wageningen, The Netherlands.
Offerhaus H; Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, The Netherlands.
Wu WY; Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.
Depken M; Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Delft, The Netherlands.
Martens KJA; Laboratory of Biophysics, Wageningen University & Research, Wageningen, The Netherlands.
Staals RHJ; Laboratory of Microbiology, Wageningen University & Research, Wageningen, The Netherlands.
Hohlbein J; Laboratory of Biophysics, Wageningen University & Research, Wageningen, The Netherlands.; Microspectroscopy Research Facility, Wageningen University & Research, Wageningen, The Netherlands.
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Źródło:
Nucleic acids research [Nucleic Acids Res] 2024 May 22; Vol. 52 (9), pp. 5241-5256.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Associated Protein 9*/metabolism
CRISPR-Associated Protein 9*/genetics
CRISPR-Associated Proteins*/metabolism
CRISPR-Associated Proteins*/genetics
CRISPR-Cas Systems*
Gene Editing*/methods
Bacterial Proteins/metabolism ; Bacterial Proteins/genetics ; DNA/metabolism ; DNA/genetics ; DNA/chemistry ; Endodeoxyribonucleases/metabolism ; Endodeoxyribonucleases/genetics ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Kinetics ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism
Czasopismo naukowe
Tytuł:
Determinants of CRISPR Cas12a nuclease activation by DNA and RNA targets.
Autorzy:
Nalefski EA; Global Health Labs, Inc, Bellevue, WA 98007, USA.
Kooistra RM; Global Health Labs, Inc, Bellevue, WA 98007, USA.
Parikh I; Global Health Labs, Inc, Bellevue, WA 98007, USA.
Hedley S; Global Health Labs, Inc, Bellevue, WA 98007, USA.
Rajaraman K; Global Health Labs, Inc, Bellevue, WA 98007, USA.
Madan D; Global Health Labs, Inc, Bellevue, WA 98007, USA.
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Źródło:
Nucleic acids research [Nucleic Acids Res] 2024 May 08; Vol. 52 (8), pp. 4502-4522.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
MeSH Terms:
CRISPR-Associated Proteins*/metabolism
CRISPR-Cas Systems*
DNA, Single-Stranded*/metabolism
Endodeoxyribonucleases*/metabolism
Endodeoxyribonucleases*/genetics
RNA*/metabolism
RNA*/chemistry
RNA*/genetics
Bacterial Proteins/metabolism ; Bacterial Proteins/genetics ; DNA/metabolism ; DNA/genetics ; DNA/chemistry ; Enzyme Activation ; Kinetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; RNA, Guide, CRISPR-Cas Systems/genetics
Czasopismo naukowe
Tytuł:
FTH1 overexpression using a dCasRx translation enhancement system protects the kidney from calcium oxalate crystal-induced injury.
Autorzy:
He Z; Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People's Republic of China.
Dong C; Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People's Republic of China.
Song T; Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People's Republic of China.
Zhou J; Department of Urology, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People's Republic of China.
Xu T; Department of Urology, Huanggang Central Hospital of Yangtze University, Huanggang, 438000, Hubei, People's Republic of China.
He R; Department of Thoracic Surgery, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, People's Republic of China. hry_.
Li S; Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, 430071, Hubei, People's Republic of China. .
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Źródło:
Cellular & molecular biology letters [Cell Mol Biol Lett] 2024 May 07; Vol. 29 (1), pp. 65. Date of Electronic Publication: 2024 May 07.
Typ publikacji:
Journal Article
MeSH Terms:
Calcium Oxalate*/metabolism
CRISPR-Cas Systems*/genetics
Kidney*/metabolism
Kidney*/pathology
Animals ; Humans ; Male ; Mice ; Eukaryotic Initiation Factor-4G/metabolism ; Eukaryotic Initiation Factor-4G/genetics ; Ferritins ; Ferroptosis/genetics ; Gene Editing/methods ; HEK293 Cells ; Kidney Calculi/genetics ; Kidney Calculi/metabolism ; Oxidoreductases/metabolism ; Oxidoreductases/genetics ; Protein Biosynthesis/genetics ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism
Czasopismo naukowe
Tytuł:
Harnessing noncanonical crRNA for highly efficient genome editing.
Autorzy:
Xun G; Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA.; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA.
Zhu Z; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA.; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA.
Singh N; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA.
Lu J; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA.
Jain PK; Department of Chemical Engineering, University of Florida, Gainesville, FL, 32611, USA.
Zhao H; Department of Bioengineering, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA. .; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA. .; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Champaign, IL, 61801, USA. .
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Źródło:
Nature communications [Nat Commun] 2024 May 07; Vol. 15 (1), pp. 3823. Date of Electronic Publication: 2024 May 07.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Gene Editing*/methods
CRISPR-Cas Systems*
Humans ; HEK293 Cells ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; RNA/genetics ; RNA/metabolism ; CRISPR-Associated Proteins/metabolism ; CRISPR-Associated Proteins/genetics ; Bacterial Proteins ; Endodeoxyribonucleases
Czasopismo naukowe
Tytuł:
CRISPR-Cas9 and Cas12a target site richness reflects genomic diversity in natural populations of Anopheles gambiae and Aedes aegypti mosquitoes.
Autorzy:
Collier TC; Independent researcher, Vero Beach, FL, 32962, USA.
Lee Y; Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32962, USA.
Mathias DK; Florida Medical Entomology Laboratory, Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32962, USA.
Del Amo VL; Center for Infectious Diseases, Department of Epidemiology, Human Genetics, and Environmental Sciences, School of Public Health, University of Texas Health Science Center, Houston, TX, 77030, USA. .
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Źródło:
BMC genomics [BMC Genomics] 2024 Jul 17; Vol. 25 (1), pp. 700. Date of Electronic Publication: 2024 Jul 17.
Typ publikacji:
Journal Article
MeSH Terms:
Anopheles*/genetics
CRISPR-Cas Systems*
Aedes*/genetics
Animals ; Genetic Variation ; RNA, Guide, CRISPR-Cas Systems/genetics ; Endodeoxyribonucleases/genetics ; Endodeoxyribonucleases/metabolism ; CRISPR-Associated Proteins/genetics ; CRISPR-Associated Proteins/metabolism ; Genome, Insect ; Mosquito Vectors/genetics ; Gene Editing ; Bacterial Proteins
Czasopismo naukowe
Tytuł:
CRISPR-Cas9 high-throughput screening to study drug resistance in Leishmania infantum .
Autorzy:
Queffeulou M; Centre de Recherche en Infectiologie du Centre de Recherche du CHU Québec, Université Laval, Québec, Canada.; Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Canada.
Leprohon P; Centre de Recherche en Infectiologie du Centre de Recherche du CHU Québec, Université Laval, Québec, Canada.; Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Canada.
Fernandez-Prada C; Centre de Recherche en Infectiologie du Centre de Recherche du CHU Québec, Université Laval, Québec, Canada.; Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Canada.
Ouellette M; Centre de Recherche en Infectiologie du Centre de Recherche du CHU Québec, Université Laval, Québec, Canada.; Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Canada.
Mejía-Jaramillo AM; Centre de Recherche en Infectiologie du Centre de Recherche du CHU Québec, Université Laval, Québec, Canada.; Département de Microbiologie, Infectiologie et Immunologie, Faculté de Médecine, Université Laval, Québec, Canada.
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Źródło:
MBio [mBio] 2024 Jul 17; Vol. 15 (7), pp. e0047724. Date of Electronic Publication: 2024 Jun 12.
Typ publikacji:
Journal Article
MeSH Terms:
Leishmania infantum*/genetics
Leishmania infantum*/drug effects
CRISPR-Cas Systems*
Drug Resistance*/genetics
Antiprotozoal Agents*/pharmacology
High-Throughput Screening Assays*/methods
Phosphorylcholine/pharmacology ; Phosphorylcholine/analogs & derivatives ; Amphotericin B/pharmacology ; RNA, Guide, CRISPR-Cas Systems/genetics ; Genome, Protozoan
Czasopismo naukowe
Tytuł:
Antagonistic conflict between transposon-encoded introns and guide RNAs.
Autorzy:
Žedaveinytė R; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Meers C; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Le HC; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Mortman EE; Department of Genetics and Development, Columbia University, New York, NY 10032, USA.
Tang S; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Lampe GD; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Pesari SR; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Gelsinger DR; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Wiegand T; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
Sternberg SH; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
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Źródło:
Science (New York, N.Y.) [Science] 2024 Jul 12; Vol. 385 (6705), pp. eadm8189. Date of Electronic Publication: 2024 Jul 12.
Typ publikacji:
Journal Article
MeSH Terms:
DNA Transposable Elements*
Introns*
RNA, Guide, CRISPR-Cas Systems*/genetics
Clostridium botulinum*/genetics
Bacterial Proteins*/genetics
Bacterial Proteins*/metabolism
Endodeoxyribonucleases*/genetics
Endodeoxyribonucleases*/metabolism
CRISPR-Associated Proteins*/genetics
CRISPR-Associated Proteins*/metabolism
CRISPR-Cas Systems ; Homologous Recombination ; RNA Splicing ; Transposases/metabolism ; Transposases/genetics
Czasopismo naukowe
Tytuł:
Generating, modeling and evaluating a large-scale set of CRISPR/Cas9 off-target sites with bulges.
Autorzy:
Yaish O; School of Electrical and Computer Engineering, Ben-Gurion University of the Negev, Beer Sheva 8410501, Israel.
Orenstein Y; Department of Computer Science, Bar-Ilan University, Ramat Gan 5290002, Israel.; The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat Gan 5290002, Israel.
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Źródło:
Nucleic acids research [Nucleic Acids Res] 2024 Jul 08; Vol. 52 (12), pp. 6777-6790.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
Gene Editing*/methods
Machine Learning*
Humans ; RNA, Guide, CRISPR-Cas Systems/genetics
Czasopismo naukowe
Tytuł:
Direct Parental (DIPA) CRISPR in the jewel wasp, Nasonia vitripennis.
Autorzy:
Zhang X; Department of Natural Sciences, Pitzer College and Scripps College, 925 N Mills Ave, Claremont, CA 91711, USA.
Singh A; Department of Natural Sciences, Pitzer College and Scripps College, 925 N Mills Ave, Claremont, CA 91711, USA.
Soriano Martinez K; Department of Natural Sciences, Pitzer College and Scripps College, 925 N Mills Ave, Claremont, CA 91711, USA.
Ferree PM; Department of Natural Sciences, Pitzer College and Scripps College, 925 N Mills Ave, Claremont, CA 91711, USA.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2024 Jul 08; Vol. 14 (7).
Typ publikacji:
Journal Article
MeSH Terms:
Wasps*/genetics
CRISPR-Cas Systems*
Gene Editing*/methods
Clustered Regularly Interspaced Short Palindromic Repeats*
Animals ; RNA, Guide, CRISPR-Cas Systems
Czasopismo naukowe
Tytuł:
LncRNAway: a web-based sgRNA design tool for precise and effective suppression of long noncoding RNAs.
Autorzy:
Zhang S; Medical Research Center, Chongqing General Hospital, Chongqing University, Chongqing 401147, China.
Wang S; China State Key Laboratory of Chemical Oncogenomics, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China.; The Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China.
Lu F; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing 400715, China.
Bie L; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing 400715, China.
Luo Y; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing 400715, China.
Sun J; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing 400715, China.
Zhang Y; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing 400715, China.
Wang Y; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Chongqing 400715, China.
Zhang Y; China State Key Laboratory of Chemical Oncogenomics, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China.; The Institute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Shenzhen 518055, China.
Lyu QR; Medical Research Center, Chongqing General Hospital, Chongqing University, Chongqing 401147, China.
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Źródło:
Nucleic acids research [Nucleic Acids Res] 2024 Jul 05; Vol. 52 (W1), pp. W95-W101.
Typ publikacji:
Journal Article
MeSH Terms:
RNA, Long Noncoding*/genetics
RNA, Long Noncoding*/metabolism
Internet*
Software*
RNA, Guide, CRISPR-Cas Systems*/genetics
Humans ; Gene Knockout Techniques ; CRISPR-Cas Systems
Czasopismo naukowe
Tytuł:
Testing multiplexed anti-ASFV CRISPR-Cas9 in reducing African swine fever virus.
Autorzy:
Zheng Z; South China Agricultural University, Guangzhou, China.
Xu L; Qihan Biotechnology, Hangzhou, China.
Gao Y; Qihan Biotechnology, Hangzhou, China.
Dou H; Qihan Biotechnology, Hangzhou, China.
Zhou Y; Qihan Biotechnology, Hangzhou, China.
Feng X; Qihan Biotechnology, Hangzhou, China.
He X; Qihan Biotechnology, Hangzhou, China.
Tian Z; Qihan Biotechnology, Hangzhou, China.
Song L; Qihan Biotechnology, Hangzhou, China.
Mo G; Qihan Biotechnology, Hangzhou, China.
Hu J; Qihan Biotechnology, Hangzhou, China.
Zhao H; Yunan Agriculture University, Kunming, China.
Wei H; Yunan Agriculture University, Kunming, China.
Church GM; Harvard University, Cambridge, Massachusetts, USA.
Yang L; Qihan Biotechnology, Hangzhou, China.
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Źródło:
Microbiology spectrum [Microbiol Spectr] 2024 Jul 02; Vol. 12 (7), pp. e0216423. Date of Electronic Publication: 2024 Apr 02.
Typ publikacji:
Journal Article
MeSH Terms:
African Swine Fever Virus*/genetics
CRISPR-Cas Systems*
African Swine Fever*/virology
African Swine Fever*/immunology
African Swine Fever*/prevention & control
Gene Editing*/methods
Virus Replication*/genetics
Animals ; Swine ; Animals, Genetically Modified/genetics ; RNA, Guide, CRISPR-Cas Systems/genetics ; Genome, Viral/genetics
Czasopismo naukowe

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