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Tytuł:
Single Molecule FRET Analysis of CRISPR Cas9 Single Guide RNA Folding Dynamics.
Autorzy:
Okafor IC; Department of Biology, Johns Hopkins University, Baltimore, Maryland 21218, United States.
Ha T; Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, United States.; Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States.; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States.; Howard Hughes Medical Institute, Baltimore, Maryland 21205, United States.
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Źródło:
The journal of physical chemistry. B [J Phys Chem B] 2023 Jan 12; Vol. 127 (1), pp. 45-51. Date of Electronic Publication: 2022 Dec 23.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
CRISPR-Cas Systems*
Gene Editing*/methods
RNA, Guide, CRISPR-Cas Systems ; Fluorescence Resonance Energy Transfer ; DNA/metabolism
Czasopismo naukowe
Szczegóły
Tytuł:
Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA.
Autorzy:
Dmytrenko O; Helmholtz Institute for RNA-based Infection Research, Helmholtz Centre for Infection Research, Würzburg, Germany.
Neumann GC; Benson Hill, St Louis, MO, USA.
Hallmark T; Department of Chemistry and Biochemistry, Utah State University, Logan, UT, USA.
Keiser DJ; Department of Chemistry and Biochemistry, Utah State University, Logan, UT, USA.
Crowley VM; Department of Chemistry and Biochemistry, Utah State University, Logan, UT, USA.
Vialetto E; Helmholtz Institute for RNA-based Infection Research, Helmholtz Centre for Infection Research, Würzburg, Germany.
Mougiakos I; Helmholtz Institute for RNA-based Infection Research, Helmholtz Centre for Infection Research, Würzburg, Germany.
Wandera KG; Helmholtz Institute for RNA-based Infection Research, Helmholtz Centre for Infection Research, Würzburg, Germany.
Domgaard H; Department of Chemistry and Biochemistry, Utah State University, Logan, UT, USA.
Weber J; Helmholtz Institute for RNA-based Infection Research, Helmholtz Centre for Infection Research, Würzburg, Germany.
Gaudin T; Helmholtz Institute for RNA-based Infection Research, Helmholtz Centre for Infection Research, Würzburg, Germany.
Metcalf J; Department of Chemistry and Biochemistry, Utah State University, Logan, UT, USA.
Gray BN; Benson Hill, St Louis, MO, USA.; Syngenta, Research Triangle Park, NC, USA.
Begemann MB; Benson Hill, St Louis, MO, USA. .
Jackson RN; Department of Chemistry and Biochemistry, Utah State University, Logan, UT, USA. .
Beisel CL; Helmholtz Institute for RNA-based Infection Research, Helmholtz Centre for Infection Research, Würzburg, Germany. .; Medical Faculty, University of Würzburg, Würzburg, Germany. .
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Źródło:
Nature [Nature] 2023 Jan; Vol. 613 (7944), pp. 588-594. Date of Electronic Publication: 2023 Jan 04.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
MeSH Terms:
CRISPR-Associated Proteins*/metabolism
CRISPR-Cas Systems*
DNA*/metabolism
RNA*/metabolism
DNA, Single-Stranded/metabolism ; SOS Response, Genetics ; DNA Damage ; RNA, Guide, CRISPR-Cas Systems ; Gene Editing
Czasopismo naukowe
Szczegóły
Tytuł:
Maximizing CRISPRi efficacy and accessibility with dual-sgRNA libraries and optimal effectors.
Autorzy:
Replogle JM; Medical Scientist Training Program, University of California, San Francisco, San Francisco, United States.; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States.; Whitehead Institute for Biomedical Research, Cambridge, United States.
Bonnar JL; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States.; Whitehead Institute for Biomedical Research, Cambridge, United States.
Pogson AN; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States.; Whitehead Institute for Biomedical Research, Cambridge, United States.
Liem CR; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.
Maier NK; Department of Microbiology, Harvard Medical School, Boston, United States.
Ding Y; Department of Microbiology, Harvard Medical School, Boston, United States.
Russell BJ; Department of Microbiology, Harvard Medical School, Boston, United States.
Wang X; Department of Microbiology, Harvard Medical School, Boston, United States.
Leng K; Medical Scientist Training Program, University of California, San Francisco, San Francisco, United States.; Institute for Neurodegenerative Disease, University of California, San Francisco, San Francisco, United States.
Guna A; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.; Whitehead Institute for Biomedical Research, Cambridge, United States.
Norman TM; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.
Pak RA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.
Ramos DM; Center for Alzheimer's Disease and Related Dementias, National Institutes of Health, Bethesda, United States.; National Institute on Aging, National Institutes of Health, Bethesda, United States.
Ward ME; National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States.
Gilbert LA; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.; Department of Urology and Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, United States.; Arc Institute, Palo Alto, United States.
Kampmann M; Institute for Neurodegenerative Disease, University of California, San Francisco, San Francisco, United States.; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States.
Weissman JS; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.; Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, United States.; Whitehead Institute for Biomedical Research, Cambridge, United States.; Department of Biology, Massachusetts Institute of Technology, Cambridge, United States.; David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, United States.
Jost M; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, United States.; Department of Microbiology, Harvard Medical School, Boston, United States.
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Źródło:
ELife [Elife] 2022 Dec 28; Vol. 11. Date of Electronic Publication: 2022 Dec 28.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, N.I.H., Extramural
MeSH Terms:
RNA, Guide, CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats*
Cell Line ; CRISPR-Cas Systems
Czasopismo naukowe
Szczegóły
Tytuł:
Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage.
Autorzy:
Jianwei L; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Jobichen C; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Machida S; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Meng S; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Read RJ; Department of Haematology, University of Cambridge, Cambridge, United Kingdom.
Hongying C; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Jian S; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Yuan YA; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Sivaraman J; Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
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Źródło:
PLoS biology [PLoS Biol] 2023 Mar 14; Vol. 21 (3), pp. e3002023. Date of Electronic Publication: 2023 Mar 14 (Print Publication: 2023).
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
RNA, Guide, CRISPR-Cas Systems*
DNA Cleavage ; RNA/chemistry ; DNA/chemistry ; Bacterial Proteins/metabolism
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Adsorption-Free Self-Priming Direct Digital Dual-crRNA CRISPR/Cas12a-Assisted Chip for Ultrasensitive Detection of Pathogens.
Autorzy:
Xia L; School of Information and Electrical Engineering, Zhejiang University City College, Hangzhou, Zhejiang Province 310015, China.; Research Centre for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province 310027, China.
Yin J; School of Information and Electrical Engineering, Zhejiang University City College, Hangzhou, Zhejiang Province 310015, China.; Research Centre for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province 310027, China.
Zhuang J; Department of Clinical Pharmacology, Key Laboratory of Clinical Cancer Pharmacology and Toxicology Research of Zhejiang Province, Affiliated Hangzhou First People's Hospital, Cancer Center, Zhejiang University School of Medicine, Hangzhou 310006, China.
Yin W; Research Centre for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province 310027, China.
Zou Z; Research Centre for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province 310027, China.
Mu Y; Research Centre for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, Zhejiang Province 310027, China.; Huzhou Institute of Zhejiang University, Huzhou 313002, China.
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Źródło:
Analytical chemistry [Anal Chem] 2023 Mar 14; Vol. 95 (10), pp. 4744-4752. Date of Electronic Publication: 2023 Mar 03.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*/genetics
RNA, Guide, CRISPR-Cas Systems*
Adsorption ; Biological Assay ; Cell Nucleus ; Nucleic Acid Amplification Techniques
Czasopismo naukowe
Szczegóły
Tytuł:
Application of CRISPR Cas systems in DNA recorders and writers.
Autorzy:
Sen D; Department of Microbiology, University of Kalyani, Nadia, West Bengal, 741235, India. Electronic address: .
Mukhopadhyay P; Department of Microbiology, Barrackpore Rastraguru Surendranath College, Barrackpore, Kolkata, West Bengal, 700120, India. Electronic address: .
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Źródło:
Bio Systems [Biosystems] 2023 Mar; Vol. 225, pp. 104870. Date of Electronic Publication: 2023 Feb 25.
Typ publikacji:
Journal Article; Review
MeSH Terms:
CRISPR-Cas Systems*/genetics
Prokaryotic Cells*
DNA/genetics
Czasopismo naukowe
Szczegóły
Tytuł:
Antiviral signalling by a cyclic nucleotide activated CRISPR protease.
Autorzy:
Rouillon C; Institute of Structural Biology, University of Bonn, Bonn, Germany. .; Max Planck Institute for Neurobiology of Behavior-caesar, Bonn, Germany. .
Schneberger N; Institute of Structural Biology, University of Bonn, Bonn, Germany.
Chi H; School of Biology, University of St Andrews, St Andrews, UK.
Blumenstock K; Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn and University Hospital Bonn, Bonn, Germany.
Da Vela S; European Molecular Biology Laboratory (EMBL), Hamburg Site, Hamburg, Germany.
Ackermann K; EaStCHEM School of Chemistry, Biomedical Sciences Research Complex, and Centre of Magnetic Resonance, University of St Andrews, North Haugh, St Andrews, UK.
Moecking J; Institute of Structural Biology, University of Bonn, Bonn, Germany.
Peter MF; Institute of Structural Biology, University of Bonn, Bonn, Germany.
Boenigk W; Max Planck Institute for Neurobiology of Behavior-caesar, Bonn, Germany.
Seifert R; Max Planck Institute for Neurobiology of Behavior-caesar, Bonn, Germany.
Bode BE; EaStCHEM School of Chemistry, Biomedical Sciences Research Complex, and Centre of Magnetic Resonance, University of St Andrews, North Haugh, St Andrews, UK.
Schmid-Burgk JL; Institute of Clinical Chemistry and Clinical Pharmacology, University of Bonn and University Hospital Bonn, Bonn, Germany.
Svergun D; European Molecular Biology Laboratory (EMBL), Hamburg Site, Hamburg, Germany.
Geyer M; Institute of Structural Biology, University of Bonn, Bonn, Germany.
White MF; School of Biology, University of St Andrews, St Andrews, UK.
Hagelueken G; Institute of Structural Biology, University of Bonn, Bonn, Germany. .
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Źródło:
Nature [Nature] 2023 Feb; Vol. 614 (7946), pp. 168-174. Date of Electronic Publication: 2022 Nov 24.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Associated Proteins*/metabolism
CRISPR-Cas Systems*/genetics
CRISPR-Cas Systems*/physiology
Nucleotides, Cyclic*/immunology
Nucleotides, Cyclic*/metabolism
Protease La*/chemistry
Protease La*/metabolism
Bacteriophages*/immunology
Bacteriophages*/metabolism
Bacteria*/enzymology
Bacteria*/immunology
Bacteria*/metabolism
Bacteria*/virology
Sigma Factor ; Operon ; Cyclic AMP/analogs & derivatives ; Cyclic AMP/chemistry ; Enzyme Activation ; Transcription, Genetic ; Gene Expression Regulation, Bacterial ; RNA, Viral
Czasopismo naukowe
Szczegóły
Tytuł:
CRISPR-Cas12a-assisted elimination of the non-specific signal from non-specific amplification in the Exponential Amplification Reaction.
Autorzy:
Niu C; MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China; Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Liu J; Department of Chemistry, Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, Ontario, N2L 3G1, Canada.
Xing X; MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China.
Zhang C; MOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing, 100084, China. Electronic address: .
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Źródło:
Analytica chimica acta [Anal Chim Acta] 2023 Apr 22; Vol. 1251, pp. 340998. Date of Electronic Publication: 2023 Feb 22.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
Nucleic Acid Amplification Techniques*/methods
Czasopismo naukowe
Szczegóły
Tytuł:
Improving the on-target activity of high-fidelity Cas9 editors by combining rational design and random mutagenesis.
Autorzy:
Spasskaya DS; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Vavilov St. 32, Moscow, 119991, Russia.
Davletshin AI; Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Vavilov St. 32, Moscow, 119991, Russia.
Bachurin SS; FSBEI HE Rostov State Medical University Ministry of Health, Nakhichevanskiy Lane 29, Rostov-On-Don, 344022, Russia.
Tutyaeva VV; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Vavilov St. 32, Moscow, 119991, Russia.
Garbuz DG; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Vavilov St. 32, Moscow, 119991, Russia.
Karpov DS; Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Engelhardt Institute of Molecular Biology Russian Academy of Sciences, Vavilov St. 32, Moscow, 119991, Russia. .
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Źródło:
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Apr; Vol. 107 (7-8), pp. 2385-2401. Date of Electronic Publication: 2023 Mar 14.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
RNA, Guide, Kinetoplastida*
Humans ; Mutagenesis ; Gene Editing ; Chromatin
Czasopismo naukowe
Szczegóły
Tytuł:
Sensitive detection of viable salmonella bacteria based on tertiary cascade signal amplification via splintR ligase ligation-PCR amplification-CRISPR/Cas12a cleavage.
Autorzy:
Zhou C; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Li W; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Zhao Y; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Gu K; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Liao Z; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Guo B; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Huang Z; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Yang M; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Wei H; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Ma P; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Li C; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Li H; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Tang Y; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
Lei C; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China. Electronic address: .
Wang H; Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu, 610064, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China. Electronic address: .
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Źródło:
Analytica chimica acta [Anal Chim Acta] 2023 Apr 01; Vol. 1248, pp. 340885. Date of Electronic Publication: 2023 Jan 23.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
Food Microbiology*
Ligases*/genetics
Salmonella*/isolation & purification
Nucleic Acid Amplification Techniques/methods ; Polymerase Chain Reaction ; RNA
Czasopismo naukowe
Szczegóły
Tytuł:
A universal CRISPR/Cas12a-powered intelligent point-of-care testing platform for multiple small molecules in the healthcare, environment, and food.
Autorzy:
Zhao Y; Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Wuhan, 430062, PR China.
Wu W; Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Wuhan, 430062, PR China.
Tang X; Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Wuhan, 430062, PR China.
Zhang Q; Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Wuhan, 430062, PR China; Hubei Hongshan Laboratory, Wuhan, 430070, PR China.
Mao J; Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Wuhan, 430062, PR China; Hubei Hongshan Laboratory, Wuhan, 430070, PR China.
Yu L; Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Wuhan, 430062, PR China.
Li P; Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Wuhan, 430062, PR China; Hubei Hongshan Laboratory, Wuhan, 430070, PR China; Zhejiang Xianghu Laboratory, Hangzhou, 311231, PR China.
Zhang Z; Oil Crops Research Institute of Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Wuhan, 430062, PR China; Hubei Hongshan Laboratory, Wuhan, 430070, PR China. Electronic address: .
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Źródło:
Biosensors & bioelectronics [Biosens Bioelectron] 2023 Apr 01; Vol. 225, pp. 115102. Date of Electronic Publication: 2023 Jan 25.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
Biosensing Techniques*
Tandem Mass Spectrometry ; Aflatoxin B1 ; Capsaicin ; Coloring Agents ; Point-of-Care Testing ; Delivery of Health Care
Czasopismo naukowe
Szczegóły
Tytuł:
CRISPR-Cas engineering in food science and sustainable agriculture: recent advancements and applications.
Autorzy:
Aman Mohammadi M; Student Research Committee, Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences, Food Science and Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Maximiano MR; S-Inova Biotech, Graduate Program in Biotechnology, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil.; Centro de Análises Proteômicas e Bioquímicas, Graduate Program in Genomic Science and Biotechnology, Universidade Católica de Brasília, Brasília, DF, Brazil.
Hosseini SM; Student Research Committee, Department of Food Science and Technology, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences, Food Science and Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran. sm_.
Franco OL; S-Inova Biotech, Graduate Program in Biotechnology, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil.; Centro de Análises Proteômicas e Bioquímicas, Graduate Program in Genomic Science and Biotechnology, Universidade Católica de Brasília, Brasília, DF, Brazil.
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Źródło:
Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2023 Apr; Vol. 46 (4), pp. 483-497. Date of Electronic Publication: 2023 Jan 28.
Typ publikacji:
Journal Article; Review
MeSH Terms:
CRISPR-Cas Systems*
Gene Editing*
Food Technology ; Bacteria/genetics ; Crops, Agricultural ; Agriculture
Czasopismo naukowe
Szczegóły
Tytuł:
Strategies for generation of mice via CRISPR/HDR-mediated knock-in.
Autorzy:
Mathew SM; Central Animal Facility, Indian Institute of Science, Bangalore, 560 012, India. .
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Źródło:
Molecular biology reports [Mol Biol Rep] 2023 Apr; Vol. 50 (4), pp. 3189-3204. Date of Electronic Publication: 2023 Jan 26.
Typ publikacji:
Review; Journal Article
MeSH Terms:
CRISPR-Cas Systems*/genetics
Gene Editing*/methods
Animals ; Mice ; Recombinational DNA Repair ; Genetic Therapy ; DNA/genetics
Czasopismo naukowe
Szczegóły
Tytuł:
Elution-free DNA detection using CRISPR/Cas9-mediated light-up aptamer transcription: Toward all-in-one DNA purification and detection tube.
Autorzy:
Song J; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Song Y; Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea; Division of Nano-Bio Sensors/Chips Development, National NanoFab Center (NNFC), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Jang H; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Moon J; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea; Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Kang H; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea; Department of Chemistry, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Huh YM; Department of Radiology, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea; Department of Biochemistry & Molecular Biology, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea; Severance Biomedical Science Institute, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea; YUHS-KRIBB Medical Convergence Research Institute, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
Son HY; Department of Radiology, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea; Severance Biomedical Science Institute, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
Rho HW; Department of Radiology, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
Park M; Department of Radiology, College of Medicine, Yonsei University, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea.
Lim EK; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea; Department of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea; School of Pharmacy, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 16419, Republic of Korea.
Jung J; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea; School of Pharmacy, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 16419, Republic of Korea.
Jung Y; Department of Chemistry, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Park HG; Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
Lee KG; Division of Nano-Bio Sensors/Chips Development, National NanoFab Center (NNFC), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea. Electronic address: .
Im SG; Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea. Electronic address: .
Kang T; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea; School of Pharmacy, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 16419, Republic of Korea. Electronic address: .
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Źródło:
Biosensors & bioelectronics [Biosens Bioelectron] 2023 Apr 01; Vol. 225, pp. 115085. Date of Electronic Publication: 2023 Jan 16.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*/genetics
Biosensing Techniques*
Mice ; Animals ; DNA/analysis ; Oligonucleotides
Czasopismo naukowe
Szczegóły
Tytuł:
Microfluidics: the propellant of CRISPR-based nucleic acid detection.
Autorzy:
Chen Y; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Qian S; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Yu X; College of Life Sciences, China Jiliang University, Hangzhou 310018, China. Electronic address: .
Wu J; College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, No. 733, Jianshe 3rd Road, Hangzhou 311200, China. Electronic address: .
Xu J; Key Laboratory of Traceability for Agricultural Genetically Modified Organisms, Ministry of Agriculture and Rural Affairs, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China. Electronic address: .
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Źródło:
Trends in biotechnology [Trends Biotechnol] 2023 Apr; Vol. 41 (4), pp. 557-574. Date of Electronic Publication: 2022 Aug 18.
Typ publikacji:
Journal Article; Review
MeSH Terms:
CRISPR-Cas Systems*
Nucleic Acids*/genetics
Microfluidics
Czasopismo naukowe
Szczegóły
Tytuł:
CRISPR-interceded CHO cell line development approaches.
Autorzy:
Amiri S; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Adibzadeh S; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Ghanbari S; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Rahmani B; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Kheirandish MH; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.; Department of Medical Biotechnology, School of Advanced Technologies, Tehran University of Medical Sciences, Tehran, Iran.
Farokhi-Fard A; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Dastjerdeh MS; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Davami F; Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
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Źródło:
Biotechnology and bioengineering [Biotechnol Bioeng] 2023 Apr; Vol. 120 (4), pp. 865-902. Date of Electronic Publication: 2023 Jan 12.
Typ publikacji:
Journal Article; Review
MeSH Terms:
Gene Editing*
CRISPR-Cas Systems*
Cricetinae ; Animals ; Humans ; Cricetulus ; CHO Cells ; Recombinant Proteins/genetics
Czasopismo naukowe
Szczegóły
Tytuł:
Prime editing: A potential treatment option for β-thalassemia.
Autorzy:
Arif T; Department of Human Genetics and Molecular Biology, University of Health Sciences Lahore, Lahore, Punjab, Pakistan.
Farooq A; Department of Human Genetics and Molecular Biology, University of Health Sciences Lahore, Lahore, Punjab, Pakistan.
Ahmad FJ; Department of Human Genetics and Molecular Biology, University of Health Sciences Lahore, Lahore, Punjab, Pakistan.
Akhtar M; School of Biological Sciences, University of Punjab Lahore, Lahore, Punjab, Pakistan.
Choudhery MS; Department of Human Genetics and Molecular Biology, University of Health Sciences Lahore, Lahore, Punjab, Pakistan.
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Źródło:
Cell biology international [Cell Biol Int] 2023 Apr; Vol. 47 (4), pp. 699-713. Date of Electronic Publication: 2022 Dec 08.
Typ publikacji:
Journal Article; Review
MeSH Terms:
CRISPR-Cas Systems*
beta-Thalassemia*/genetics
Humans ; Gene Editing ; Genome
Czasopismo naukowe
Szczegóły
Tytuł:
The abortive infection functions of CRISPR-Cas and Argonaute.
Autorzy:
Chen Y; State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070 Wuhan, China.
Zeng Z; State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070 Wuhan, China.
She Q; CRISPR and Archaea Biology Research Center, State Key Laboratory of Microbial Technology, Shandong University, Binhai Road 72, Jimo, 266237, Qingdao, China.
Han W; State Key Laboratory of Agricultural Microbiology and College of Life Science and Technology, Hubei Hongshan Laboratory, Huazhong Agricultural University, 430070 Wuhan, China. Electronic address: .
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Źródło:
Trends in microbiology [Trends Microbiol] 2023 Apr; Vol. 31 (4), pp. 405-418. Date of Electronic Publication: 2022 Nov 30.
Typ publikacji:
Journal Article; Review
MeSH Terms:
CRISPR-Cas Systems*/genetics
Prokaryotic Cells*
Czasopismo naukowe
Szczegóły
Tytuł:
Research progress on nucleic acid detection and genome editing of CRISPR/Cas12 system.
Autorzy:
Yang Y; School of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu Province, People's Republic of China. .
Wang D; School of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu Province, People's Republic of China.
Lü P; School of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu Province, People's Republic of China.
Ma S; School of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu Province, People's Republic of China.
Chen K; School of Life Sciences, Jiangsu University, 301 Xuefu Road, Zhenjiang, 212013, Jiangsu Province, People's Republic of China.
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Źródło:
Molecular biology reports [Mol Biol Rep] 2023 Apr; Vol. 50 (4), pp. 3723-3738. Date of Electronic Publication: 2023 Jan 17.
Typ publikacji:
Journal Article; Review
MeSH Terms:
Gene Editing*
CRISPR-Cas Systems*/genetics
Animals ; Bacteria/genetics ; DNA, Single-Stranded/genetics ; DNA
Czasopismo naukowe
Szczegóły
Tytuł:
Collateral activity of the CRISPR/RfxCas13d system in human cells.
Autorzy:
Shi P; Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA. .
Murphy MR; Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.
Aparicio AO; Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.
Kesner JS; Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.
Fang Z; Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.
Chen Z; Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.; Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA, USA.
Trehan A; Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.
Guo Y; Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA.
Wu X; Department of Medicine and Department of Systems Biology, Columbia University Irving Medical Center, New York, NY, USA. .
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Źródło:
Communications biology [Commun Biol] 2023 Mar 28; Vol. 6 (1), pp. 334. Date of Electronic Publication: 2023 Mar 28.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
RNA*/genetics
Humans ; Transcriptome ; Bacteria/genetics
Czasopismo naukowe
Szczegóły
Wyświetlanie 1-20 z 14265

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