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Tytuł:
The MyLO CRISPR-Cas9 toolkit: a markerless yeast localization and overexpression CRISPR-Cas9 toolkit.
Autorzy:
Bean BDM; Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC H4B1R6, Canada.
Whiteway M; Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC H4B1R6, Canada.
Martin VJJ; Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montréal, QC H4B1R6, Canada.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2022 Jul 29; Vol. 12 (8).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*/genetics
Saccharomyces cerevisiae*/genetics
Gene Editing/methods ; Green Fluorescent Proteins/genetics ; RNA, Guide, CRISPR-Cas Systems/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
A homing suppression gene drive with multiplexed gRNAs maintains high drive conversion efficiency and avoids functional resistance alleles.
Autorzy:
Yang E; Department of Computational Biology, Cornell University, Ithaca, NY 14853, USA.; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Metzloff M; Department of Computational Biology, Cornell University, Ithaca, NY 14853, USA.; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Langmüller AM; Department of Computational Biology, Cornell University, Ithaca, NY 14853, USA.; Institut für Populationsgenetik, Vetmeduni Vienna, 1210 Wien, Austria.; Vienna Graduate School of Population Genetics, 1210 Wien, Austria.
Xu X; Center for Bioinformatics, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
Clark AG; Department of Computational Biology, Cornell University, Ithaca, NY 14853, USA.; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Messer PW; Department of Computational Biology, Cornell University, Ithaca, NY 14853, USA.
Champer J; Department of Computational Biology, Cornell University, Ithaca, NY 14853, USA.; Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.; Center for Bioinformatics, School of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2022 May 30; Vol. 12 (6).
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms:
Gene Drive Technology*/methods
RNA, Guide, CRISPR-Cas Systems*/genetics
Alleles ; Animals ; CRISPR-Cas Systems ; Drosophila melanogaster/genetics ; Female ; Translocation, Genetic
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
iCRISEE: an integrative analysis of CRISPR screen by reducing false positive hits.
Autorzy:
Zhang T; Tongji University Cancer Center, Shanghai Tenth People's Hospital, School of Medicine, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Li Y; School of Medicine, Tongji University, Shanghai 200092, China.
Yang Y; School of Medicine, Tongji University, Shanghai 200092, China.
Weng L; Tongji University Cancer Center, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200092, China.
Wu Z; Tongji University Cancer Center, Shanghai Tenth People's Hospital, School of Medicine, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Zhu J; School of Medicine, Tongji University, Shanghai 200092, China.
Qin J; Tongji University Cancer Center, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200092, China.
Liu Q; School of Life Sciences and Technology, Tongji University, Shanghai 200092, China.
Wang P; Tongji University Cancer Center, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai 200092, China.
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Źródło:
Briefings in bioinformatics [Brief Bioinform] 2022 Jan 17; Vol. 23 (1).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*
Gene Editing*
Algorithms ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Pipeline for generating stable large genomic deletions in zebrafish, from small domains to whole gene excisions.
Autorzy:
Tromp A; Queensland Brain Institute, The University of Queensland, St Lucia, Queensland 4072, Australia.
Robinson K; Queensland Brain Institute, The University of Queensland, St Lucia, Queensland 4072, Australia.
Hall TE; Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia.
Mowry B; Queensland Brain Institute, The University of Queensland, St Lucia, Queensland 4072, Australia.; Queensland Centre for Mental Health Research, West Moreton Hospital and Health Service, Wacol, QLD 4076 Australia.
Giacomotto J; Queensland Brain Institute, The University of Queensland, St Lucia, Queensland 4072, Australia.; Queensland Centre for Mental Health Research, West Moreton Hospital and Health Service, Wacol, QLD 4076 Australia.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2021 Dec 08; Vol. 11 (12).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*
Zebrafish*/genetics
Animals ; Exons ; Genomics ; RNA, Guide, CRISPR-Cas Systems/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Multigene editing: current approaches and beyond.
Autorzy:
Peng H; Data Science Institute, University of Technology Sydney, PO Box 123, Ultimo, NSW 2007, Australia.; School of Computing, National University of Singapore, 13 Computing Drive, 117417, Singapore.
Zheng Y; Data Science Institute, University of Technology Sydney, PO Box 123, Ultimo, NSW 2007, Australia.
Zhao Z; Data Science Institute, University of Technology Sydney, PO Box 123, Ultimo, NSW 2007, Australia.
Li J; Data Science Institute, University of Technology Sydney, PO Box 123, Ultimo, NSW 2007, Australia.
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Źródło:
Briefings in bioinformatics [Brief Bioinform] 2021 Sep 02; Vol. 22 (5).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms:
CRISPR-Cas Systems*
Gene Editing/*methods
Neoplasms/*genetics
RNA, Guide, CRISPR-Cas Systems/*genetics
Animals ; BRCA1 Protein/genetics ; BRCA1 Protein/metabolism ; BRCA2 Protein/genetics ; BRCA2 Protein/metabolism ; Bacteria/genetics ; Bacteria/metabolism ; Disease Models, Animal ; Humans ; Multigene Family ; Neoplasm Proteins/genetics ; Neoplasm Proteins/metabolism ; Neoplasms/metabolism ; Neoplasms/pathology ; Neurofibromin 1/genetics ; Neurofibromin 1/metabolism ; PTEN Phosphohydrolase/genetics ; PTEN Phosphohydrolase/metabolism ; Plants/genetics ; Plants/metabolism ; Protein Isoforms/genetics ; Protein Isoforms/metabolism ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Repressor Proteins/genetics ; Repressor Proteins/metabolism ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Implementation of dCas9-mediated CRISPRi in the fission yeast Schizosaccharomyces pombe.
Autorzy:
Ishikawa K; Department of Cell Biology, Institute of Life Science, Kurume University, Asahi-machi 67, Kurume, Fukuoka 830-0011, Japan.
Soejima S; Department of Cell Biology, Institute of Life Science, Kurume University, Asahi-machi 67, Kurume, Fukuoka 830-0011, Japan.
Masuda F; Department of Cell Biology, Institute of Life Science, Kurume University, Asahi-machi 67, Kurume, Fukuoka 830-0011, Japan.
Saitoh S; Department of Cell Biology, Institute of Life Science, Kurume University, Asahi-machi 67, Kurume, Fukuoka 830-0011, Japan.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2021 Apr 15; Vol. 11 (4).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*/genetics
Schizosaccharomyces*/genetics
Gene Expression ; Plasmids ; RNA, Guide, CRISPR-Cas Systems/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
CRISpy-Pop: A Web Tool for Designing CRISPR/Cas9-Driven Genetic Modifications in Diverse Populations.
Autorzy:
Stoneman HR; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53726.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison WI 53726.; Wisconsin Energy Institute, University of Wisconsin-Madison, WI 53726.; J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, WI 53726.
Wrobel RL; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53726.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison WI 53726.; Wisconsin Energy Institute, University of Wisconsin-Madison, WI 53726.; J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, WI 53726.
Place M; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53726.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison WI 53726.
Graham M; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53726.; Wisconsin Energy Institute, University of Wisconsin-Madison, WI 53726.
Krause DJ; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53726.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison WI 53726.; Wisconsin Energy Institute, University of Wisconsin-Madison, WI 53726.; J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, WI 53726.
De Chiara M; Université Côte d'Azur, CNRS, INSERM, IRCAN, Nice, France.
Liti G; Université Côte d'Azur, CNRS, INSERM, IRCAN, Nice, France.
Schacherer J; Université de Strasbourg, CNRS, GMGM UMR 7156, Strasbourg, France.
Landick R; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53726.; Department of Biochemistry, University of Wisconsin-Madison, WI 53706.; Department of Bacteriology, University of Wisconsin-Madison, WI 53706.
Gasch AP; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53726.; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison WI 53726.; J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, WI 53726.
Sato TK; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53726.; Wisconsin Energy Institute, University of Wisconsin-Madison, WI 53726.; J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, WI 53726.
Hittinger CT; DOE Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, WI 53726 .; Laboratory of Genetics, Center for Genomic Science Innovation, University of Wisconsin-Madison WI 53726.; Wisconsin Energy Institute, University of Wisconsin-Madison, WI 53726.; J. F. Crow Institute for the Study of Evolution, University of Wisconsin-Madison, WI 53726.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2020 Nov 05; Vol. 10 (11), pp. 4287-4294. Date of Electronic Publication: 2020 Nov 05.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
CRISPR-Cas Systems*
Gene Editing*
Clustered Regularly Interspaced Short Palindromic Repeats ; Genome, Human ; Humans ; RNA, Guide, CRISPR-Cas Systems/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Simplified homology-assisted CRISPR for gene editing in Drosophila.
Autorzy:
Rankin AE; Phillips Exeter Academy, Exeter, NH 03833, USA.
Fox E; The Lawrenceville School, Lawrenceville, NJ 08648, USA.
Chisholm T; Phillips Exeter Academy, Exeter, NH 03833, USA.
Lantz N; The Lawrenceville School, Lawrenceville, NJ 08648, USA.
Rajan A; Phillips Exeter Academy, Exeter, NH 03833, USA.
Phillips W; The Lawrenceville School, Lawrenceville, NJ 08648, USA.
Griffin E; Phillips Exeter Academy, Exeter, NH 03833, USA.
Harper J; Phillips Exeter Academy, Exeter, NH 03833, USA.
Suhr C; Phillips Exeter Academy, Exeter, NH 03833, USA.
Tan M; Phillips Exeter Academy, Exeter, NH 03833, USA.
Wang J; Phillips Exeter Academy, Exeter, NH 03833, USA.
Yang A; Phillips Exeter Academy, Exeter, NH 03833, USA.
Kim ES; Phillips Exeter Academy, Exeter, NH 03833, USA.
Ankrah NKA; The Lawrenceville School, Lawrenceville, NJ 08648, USA.
Chakraborty P; The Lawrenceville School, Lawrenceville, NJ 08648, USA.
Lam ACK; The Lawrenceville School, Lawrenceville, NJ 08648, USA.
Laws ME; The Lawrenceville School, Lawrenceville, NJ 08648, USA.
Lee J; The Lawrenceville School, Lawrenceville, NJ 08648, USA.
Park KK; The Lawrenceville School, Lawrenceville, NJ 08648, USA.
Wesel E; Stanford University, Stanford, CA 94305, USA.
Covert PH; Stanford University, Stanford, CA 94305, USA.
Kockel L; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Park S; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Kim SK; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.; Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.; Department of Pediatrics, Stanford University School of Medicine, Stanford, CA 94305, USA.; Stanford Diabetes Research Center, Stanford, CA 94305, USA.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2024 Feb 07; Vol. 14 (2).
Typ publikacji:
Journal Article
MeSH Terms:
Gene Editing*/methods
Drosophila*/genetics
Animals ; Transgenes ; Genome ; CRISPR-Cas Systems
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Analyzing CRISPR screens in non-conventional microbes.
Autorzy:
Trivedi V; Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA.
Ramesh A; Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA.
Wheeldon I; Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA.; Center for Industrial Biotechnology, University of California, Riverside, CA 92521, USA.; Integrative Institute for Genome Biology, University of California, Riverside, CA 92521, USA.
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Źródło:
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2023 Feb 17; Vol. 50 (1).
Typ publikacji:
Review; Journal Article
MeSH Terms:
CRISPR-Cas Systems*
Clustered Regularly Interspaced Short Palindromic Repeats*/genetics
Animals ; Genomics ; Genome ; Computational Biology ; Mammals/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Specific Gene Disruption in the Major Livestock Pests Cochliomyia hominivorax and Lucilia cuprina Using CRISPR/Cas9.
Autorzy:
Paulo DF; Centre for Molecular Biology and Genetic Engineering, Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas.; Laboratory of Ecological and Evolutionary Genomics, Smithsonian Tropical Research Institute, Gamboa, Panama.
Williamson ME; Department of Entomology and Plant Pathology, North Carolina State University, Raleigh NC.
Arp AP; USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, Kerrville TX, and.
Li F; Department of Entomology and Plant Pathology, North Carolina State University, Raleigh NC.
Sagel A; USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, Screwworm Research Site, Pacora, Panama.
Skoda SR; USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, Screwworm Research Site, Pacora, Panama.
Sanchez-Gallego J; USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, Screwworm Research Site, Pacora, Panama.
Vasquez M; USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, Screwworm Research Site, Pacora, Panama.
Quintero G; USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, Screwworm Research Site, Pacora, Panama.
Pérez de León AA; USDA-ARS, Knipling-Bushland U.S. Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, Kerrville TX, and.
Belikoff EJ; Department of Entomology and Plant Pathology, North Carolina State University, Raleigh NC.
Azeredo-Espin AML; Centre for Molecular Biology and Genetic Engineering, Department of Genetics, Evolution, Microbiology and Immunology, University of Campinas.
McMillan WO; Laboratory of Ecological and Evolutionary Genomics, Smithsonian Tropical Research Institute, Gamboa, Panama.
Concha C; Laboratory of Ecological and Evolutionary Genomics, Smithsonian Tropical Research Institute, Gamboa, Panama .
Scott MJ; Department of Entomology and Plant Pathology, North Carolina State University, Raleigh NC .
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2019 Sep 04; Vol. 9 (9), pp. 3045-3055. Date of Electronic Publication: 2019 Sep 04.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
CRISPR-Cas Systems*
Diptera/*genetics
Insect Proteins/*genetics
Animals ; Animals, Genetically Modified ; Diptera/embryology ; Embryo, Nonmammalian ; Female ; Male ; Mutation ; Pest Control/methods ; RNA, Guide, CRISPR-Cas Systems ; Sex Determination Processes
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
High-Efficiency CRISPR/Cas9-Mediated Gene Editing in Honeybee ( Apis mellifera ) Embryos.
Autorzy:
Hu XF; Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Zhang B; Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Liao CH; Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China.
Zeng ZJ; Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, China .
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2019 May 07; Vol. 9 (5), pp. 1759-1766. Date of Electronic Publication: 2019 May 07.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*
Embryo, Nonmammalian*
Gene Editing*
Bees/*genetics
Animals ; Base Sequence ; Gene Knockdown Techniques ; Glycoproteins/chemistry ; Glycoproteins/genetics ; Insect Proteins/chemistry ; Insect Proteins/genetics ; Mutagenesis ; Mutation ; PAX6 Transcription Factor/chemistry ; PAX6 Transcription Factor/genetics ; RNA, Guide, CRISPR-Cas Systems
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
A systematic evaluation of data processing and problem formulation of CRISPR off-target site prediction.
Autorzy:
Yaish O; School of Electrical and Computer Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel.
Asif M; School of Electrical and Computer Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel.
Orenstein Y; School of Electrical and Computer Engineering, Ben-Gurion University of the Negev, Beer Sheva, Israel.
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Źródło:
Briefings in bioinformatics [Brief Bioinform] 2022 Sep 20; Vol. 23 (5).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*
RNA, Guide, CRISPR-Cas Systems*/genetics
Gene Editing/methods ; Research Design
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Systematic investigation of CRISPR-Cas9 configurations for flexible and efficient genome editing in Corynebacterium glutamicum NRRL-B11474.
Autorzy:
Cameron Coates R; Zymergen Inc., 5980 Horton St #105, Emeryville, CA, 94608, USA.
Blaskowski S; Zymergen Inc., 5980 Horton St #105, Emeryville, CA, 94608, USA.
Szyjka S; Zymergen Inc., 5980 Horton St #105, Emeryville, CA, 94608, USA.
van Rossum HM; Zymergen Inc., 5980 Horton St #105, Emeryville, CA, 94608, USA.
Vallandingham J; Zymergen Inc., 5980 Horton St #105, Emeryville, CA, 94608, USA.
Patel K; Zymergen Inc., 5980 Horton St #105, Emeryville, CA, 94608, USA.
Serber Z; Zymergen Inc., 5980 Horton St #105, Emeryville, CA, 94608, USA.
Dean J; Zymergen Inc., 5980 Horton St #105, Emeryville, CA, 94608, USA. .
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Źródło:
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2019 Feb; Vol. 46 (2), pp. 187-201. Date of Electronic Publication: 2018 Nov 27.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
Gene Editing*
Corynebacterium glutamicum/*genetics
Gene Deletion ; Plasmids/genetics ; Polymorphism, Single Nucleotide ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/isolation & purification
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Nebulous without white: annotated long-read genome assembly and CRISPR/Cas9 genome engineering in Drosophila nebulosa.
Autorzy:
Sottolano CJ; Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.
Revaitis NT; Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.
Geneva AJ; Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.; Department of Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.
Yakoby N; Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.; Department of Biology, Rutgers, The State University of New Jersey, Camden, NJ 08103, USA.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2022 Nov 04; Vol. 12 (11).
Typ publikacji:
Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, N.I.H., Extramural
MeSH Terms:
CRISPR-Cas Systems*
Gene Editing*
Animals ; Male ; Drosophila/genetics ; Drosophila melanogaster/genetics ; Animals, Genetically Modified
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli.
Autorzy:
Li Y; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
Yan F; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Wu H; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Li G; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Han Y; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Ma Q; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
Fan X; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
Zhang C; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
Xu Q; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China.; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China.
Xie X; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. .; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China. .; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China. .
Chen N; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China. .; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science and Technology, Tianjin, 300457, China. .; Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China. .
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Źródło:
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2019 Jan; Vol. 46 (1), pp. 81-90. Date of Electronic Publication: 2018 Nov 23.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
DNA Fragmentation*
Chromosomes, Bacterial/*genetics
DNA, Bacterial/*genetics
Escherichia coli/*genetics
Base Sequence ; Cloning, Molecular ; Gene Deletion ; Gene Editing ; Genes, Bacterial ; Plasmids/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Streptococcus pyogenes/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Generation and Phenotype Identification of PAX4 Gene Knockout Rabbit by CRISPR/Cas9 System.
Autorzy:
Xu Y; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China.
Wang Y; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China.
Song Y; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China.
Deng J; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China.
Chen M; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China.
Ouyang H; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China.
Lai L; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China lizj__.
Li Z; Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University, Changchun 130062, China lizj__.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2018 Jul 31; Vol. 8 (8), pp. 2833-2840. Date of Electronic Publication: 2018 Jul 31.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*
Phenotype*
Paired Box Transcription Factors/*deficiency
Paired Box Transcription Factors/*genetics
Animals ; Animals, Genetically Modified ; Gene Expression ; Gene Knockout Techniques ; Gene Order ; Gene Targeting ; Genetic Vectors ; Immunohistochemistry ; Mutation ; Paired Box Transcription Factors/metabolism ; RNA, Guide, CRISPR-Cas Systems ; Rabbits
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
ExsgRNA: reduce off-target efficiency by on-target mismatched sgRNA.
Autorzy:
Hu WX; Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.
Rong Y; Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.
Guo Y; Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.
Jiang F; Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.
Tian W; Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.
Chen H; Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.
Dong SS; Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.; Research Institute of Xi'an Jiaotong University, Zhejiang, China.
Yang TL; Biomedical Informatics & Genomics Center, Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, P.R. China.
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Źródło:
Briefings in bioinformatics [Brief Bioinform] 2022 Sep 20; Vol. 23 (5).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*
RNA, Small Untranslated*/genetics
RNA, Small Untranslated*/metabolism
DNA ; Gene Editing/methods ; Luciferases/genetics ; Luciferases/metabolism
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Rapid and Efficient CRISPR/Cas9-Based Mating-Type Switching of Saccharomyces cerevisiae .
Autorzy:
Xie ZX; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.; Institute for Systems Genetics, NYU Langone Health, New York 10016.
Mitchell LA; Department of Biochemistry and Molecular Pharmacology NYU Langone Health, New York 10016.; Institute for Systems Genetics, NYU Langone Health, New York 10016.
Liu HM; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Li BZ; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Liu D; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Agmon N; Department of Biochemistry and Molecular Pharmacology NYU Langone Health, New York 10016.; Institute for Systems Genetics, NYU Langone Health, New York 10016.
Wu Y; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.; Institute for Systems Genetics, NYU Langone Health, New York 10016.
Li X; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Zhou X; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Li B; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Xiao WH; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Ding MZ; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Wang Y; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Yuan YJ; Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072, China.; SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 300072, China.
Boeke JD; Department of Biochemistry and Molecular Pharmacology NYU Langone Health, New York 10016 .; Institute for Systems Genetics, NYU Langone Health, New York 10016.
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Źródło:
G3 (Bethesda, Md.) [G3 (Bethesda)] 2018 Jan 04; Vol. 8 (1), pp. 173-183. Date of Electronic Publication: 2018 Jan 04.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
CRISPR-Cas Systems*
Genes, Mating Type, Fungal*
Genome, Fungal*
DNA, Fungal/*genetics
Gene Editing/*methods
Saccharomyces cerevisiae/*genetics
Cell Engineering/methods ; Chromosomes, Artificial/chemistry ; DNA Breaks, Double-Stranded ; DNA, Fungal/metabolism ; Genetic Loci ; Plasmids/chemistry ; Plasmids/metabolism ; Ploidies ; RNA, Guide, CRISPR-Cas Systems/genetics ; RNA, Guide, CRISPR-Cas Systems/metabolism ; Saccharomyces cerevisiae/metabolism
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
Simultaneous Targeting of Multiple Gene Homeologs to Alter Seed Oil Production in Camelina sativa.
Autorzy:
Aznar-Moreno JA; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66506, USA.
Durrett TP; Department of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS 66506, USA.
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Źródło:
Plant & cell physiology [Plant Cell Physiol] 2017 Jul 01; Vol. 58 (7), pp. 1260-1267.
Typ publikacji:
Journal Article
MeSH Terms:
CRISPR-Cas Systems*
Camellia/*genetics
Clustered Regularly Interspaced Short Palindromic Repeats/*genetics
Fatty Acids/*metabolism
RNA, Guide, CRISPR-Cas Systems/*metabolism
Camellia/metabolism ; Gene Editing ; Mutation ; Phylogeny ; Plant Oils/metabolism ; Plants, Genetically Modified ; Polyploidy ; RNA, Guide, CRISPR-Cas Systems/genetics ; Seeds/genetics ; Seeds/metabolism
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknieSzczegóły
Tytuł:
GuideMaker: Software to design CRISPR-Cas guide RNA pools in non-model genomes.
Autorzy:
Poudel R; Genomics and Bioinformatics Research Unit, USDA Agricultural Research Service, Gainesville, FL 32608, USA.; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32601, USA.
Rodriguez LT; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32601, USA.
Reisch CR; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32601, USA.
Rivers AR; Genomics and Bioinformatics Research Unit, USDA Agricultural Research Service, Gainesville, FL 32608, USA.
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Źródło:
GigaScience [Gigascience] 2022 Apr 01; Vol. 11.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
CRISPR-Cas Systems*
RNA, Guide, CRISPR-Cas Systems*/genetics
Gene Editing ; Genome ; Software
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Wyświetlanie 1-20 z 164

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