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Wyszukujesz frazę ""Cell Culture Techniques"" wg kryterium: Temat


Tytuł :
Bioreactor-Based Antigen Production Process Using the Baculovirus Expression Vector System.
Autorzy :
Harnischfeger J; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Käßer L; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Zitzmann J; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Salzig D; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany.
Czermak P; Institute of Bioprocess Engineering and Pharmaceutical Technology (IBPT), Technische Hochschule Mittelhessen (THM) - University of Applied Sciences, Giessen, Germany. .; Faculty of Biology and Chemistry, Justus-Liebig-University Giessen, Giessen, Germany. .; Division Bioresources, Fraunhofer Institute for Molecular Biology and Applied Ecology (IME), Giessen, Germany. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2183, pp. 95-118.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Batch Cell Culture Techniques*
Bioreactors*
Recombinant Proteins*
Antigens/*biosynthesis
Antigens/*genetics
Baculoviridae/*genetics
Genetic Vectors/*genetics
Animals ; Antigens/isolation & purification ; Cell Culture Techniques ; Cloning, Molecular ; Gene Expression ; Genetic Engineering ; Sf9 Cells ; Transfection ; Workflow
Czasopismo naukowe
Tytuł :
Production of Chimeric Hepatitis B Virus Surface Antigens in Mammalian Cells.
Autorzy :
Dobrica MO; Institute of Biochemistry of the Romanian Academy, Bucharest, Romania.
Lazar C; Institute of Biochemistry of the Romanian Academy, Bucharest, Romania.
Branza-Nichita N; Institute of Biochemistry of the Romanian Academy, Bucharest, Romania. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2183, pp. 83-94.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Batch Cell Culture Techniques*
Recombinant Fusion Proteins*
Hepatitis B Surface Antigens/*biosynthesis
Animals ; Cell Culture Techniques ; Gene Expression ; HEK293 Cells ; Hepatitis B Surface Antigens/genetics ; Hepatitis B Surface Antigens/immunology ; Hepatitis B Surface Antigens/isolation & purification ; Hepatitis B Vaccines/biosynthesis ; Hepatitis B Vaccines/immunology ; Humans ; Transfection
Czasopismo naukowe
Tytuł :
Production of Zika Virus Virus-Like Particles.
Autorzy :
Kuadkitkan A; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Ramphan S; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Worawichawong S; Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.
Sornjai W; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Wikan N; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand.
Smith DR; Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2183, pp. 183-203.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Batch Cell Culture Techniques*
Vaccines, Virus-Like Particle/*biosynthesis
Vaccines, Virus-Like Particle/*immunology
Zika Virus/*immunology
Cell Culture Techniques ; Cloning, Molecular ; Gene Expression ; Genetic Engineering ; Genetic Vectors/genetics ; HEK293 Cells ; Humans ; Plasmids/genetics ; Vaccines, Virus-Like Particle/isolation & purification ; Vaccines, Virus-Like Particle/ultrastructure
Czasopismo naukowe
Tytuł :
Effect of low-level ultrasound treatment on the production of L-leucine by Corynebacterium glutamicum in fed-batch culture.
Autorzy :
Zhang Y; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science & Technology, Tianjin, PR China.; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, PR China.
Chen Z; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science & Technology, Tianjin, PR China.; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, PR China.
Sun P; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science & Technology, Tianjin, PR China.; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, PR China.
Xu Q; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science & Technology, Tianjin, PR China.; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, PR China.
Chen N; National and Local United Engineering Lab of Metabolic Control Fermentation Technology, Tianjin University of Science & Technology, Tianjin, PR China.; College of Biotechnology, Tianjin University of Science & Technology, Tianjin, PR China.
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Źródło :
Bioengineered [Bioengineered] 2021 Dec; Vol. 12 (1), pp. 1078-1090.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Batch Cell Culture Techniques*
Ultrasonics*
Corynebacterium glutamicum/*metabolism
Leucine/*biosynthesis
Biomass ; Cell Membrane Permeability ; Corynebacterium glutamicum/cytology ; Corynebacterium glutamicum/ultrastructure ; Fermentation ; Glucose/analysis ; Leucine/analysis ; Time Factors
Czasopismo naukowe
Tytuł :
Thin fluorinated polymer film microcavity arrays for 3D cell culture and label-free automated feature extraction.
Autorzy :
Samal P; MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, The Netherlands. .
Gubbins E; MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, The Netherlands. .
van Blitterswijk C; MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, The Netherlands. .
Truckenmüller R; MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, The Netherlands. .
Giselbrecht S; MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Universiteitssingel 40, 6229 ER Maastricht, The Netherlands. .
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Źródło :
Biomaterials science [Biomater Sci] 2021 Nov 23; Vol. 9 (23), pp. 7838-7850. Date of Electronic Publication: 2021 Nov 23.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Fluorocarbon Polymers*
Animals ; Drug Discovery ; Mice ; Stem Cells
Czasopismo naukowe
Tytuł :
Establishment of myoblast cell line and identification of key genes regulating myoblast differentiation in a marine teleost, Sebastes schlegelii.
Autorzy :
Kong X; MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Wang X; MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Li M; MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Song W; MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Huang K; MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Zhang F; MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
Zhang Q; MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering, Sanya Oceanographic Institution, Ocean University of China, Sanya, China.
Qi J; MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering, Sanya Oceanographic Institution, Ocean University of China, Sanya, China.
He Y; MOE Key Laboratory of Molecular Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering, Sanya Oceanographic Institution, Ocean University of China, Sanya, China. Electronic address: .
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Źródło :
Gene [Gene] 2021 Nov 15; Vol. 802, pp. 145869. Date of Electronic Publication: 2021 Aug 02.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Cell Line*
Muscle Development/*genetics
Myoblasts, Skeletal/*physiology
Perciformes/*anatomy & histology
Animals ; Cryopreservation ; Transcriptome
Czasopismo naukowe
Tytuł :
Non-coating method for non-adherent cell culture using high molecular weight dextran sulfate and bovine serum albumin.
Autorzy :
Kikuchi T; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan. Electronic address: .
Matsuura K; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
Shimizu T; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University (TWIns), 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.
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Źródło :
Journal of bioscience and bioengineering [J Biosci Bioeng] 2021 Nov; Vol. 132 (5), pp. 537-542. Date of Electronic Publication: 2021 Sep 10.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Serum Albumin, Bovine*
Adsorption ; Cell Adhesion ; Dextran Sulfate ; Molecular Weight ; Surface Properties
Czasopismo naukowe
Tytuł :
A superhydrophobic chip integrated with an array of medium reservoirs for long-term hanging drop spheroid culture.
Autorzy :
Sun B; Key Laboratory of Optoelectronic Technology and Systems, Ministry of Education, Defence Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University, Chongqing 400044, China.
Zhao Y; Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital, Chongqing, 400030, China.
Wu W; School of Mechanical and Power Engineering, Chongqing University of Science and Technology, Chongqing 401331, China.
Zhao Q; Key Laboratory of Optoelectronic Technology and Systems, Ministry of Education, Defence Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University, Chongqing 400044, China.
Li G; Key Laboratory of Optoelectronic Technology and Systems, Ministry of Education, Defence Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University, Chongqing 400044, China. Electronic address: .
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Źródło :
Acta biomaterialia [Acta Biomater] 2021 Nov; Vol. 135, pp. 234-242. Date of Electronic Publication: 2021 Aug 10.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Culture Techniques*
Spheroids, Cellular*
Culture Media ; Tissue Engineering ; Wettability
Czasopismo naukowe
Tytuł :
Equal mixing time enables scale-down and optimization of a CHO cell culture process using a shaken microbioreactor system.
Autorzy :
Wiegmann V; The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Gordon Street, London, WC1E 6BT, UK.
Gardner RA; Ludger Ltd, Culham Science Centre, Abingdon, Oxfordshire, UK.
Spencer DIR; Ludger Ltd, Culham Science Centre, Abingdon, Oxfordshire, UK.
Baganz F; The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Gordon Street, London, WC1E 6BT, UK.
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Źródło :
Biotechnology journal [Biotechnol J] 2021 Nov; Vol. 16 (11), pp. e2100360. Date of Electronic Publication: 2021 Sep 21.
Typ publikacji :
Journal Article
MeSH Terms :
Bioreactors*
Cell Culture Techniques*
Animals ; Biotechnology ; CHO Cells ; Cricetinae ; Cricetulus
Czasopismo naukowe
Tytuł :
Development of 3D cultures of zebrafish liver and embryo cell lines: a comparison of different spheroid formation methods.
Autorzy :
de Souza IR; Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), Paraná, Brazil.
Canavez ADPM; Grupo Boticário, R&D Department, São José dos Pinhais, Paraná, Brazil.
Schuck DC; Grupo Boticário, R&D Department, São José dos Pinhais, Paraná, Brazil.
Gagosian VSC; Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), Paraná, Brazil.
de Souza IR; Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), Paraná, Brazil.
Vicari T; Graduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), Paraná, Brazil.
da Silva Trindade E; Department of Cell Biology, Federal University of Paraná (UFPR), Paraná, Brazil.
Cestari MM; Department of Genetics, Federal University of Paraná (UFPR), Paraná, Brazil.
Lorencini M; Grupo Boticário, R&D Department, São José dos Pinhais, Paraná, Brazil.
Leme DM; Department of Genetics, Federal University of Paraná (UFPR), Paraná, Brazil. .
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Źródło :
Ecotoxicology (London, England) [Ecotoxicology] 2021 Nov; Vol. 30 (9), pp. 1893-1909. Date of Electronic Publication: 2021 Aug 11.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Zebrafish*
Animals ; Cell Line, Tumor ; Liver ; Spheroids, Cellular
Czasopismo naukowe
Tytuł :
Droplet contact-based spheroid transfer technique as a multi-step assay tool for spheroid arrays.
Autorzy :
Kim H; Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. .
Roh H; Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. .
Kim H; Synthetic Biology and Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Park JK; Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. .; KAIST Institute for Health Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
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Źródło :
Lab on a chip [Lab Chip] 2021 Oct 26; Vol. 21 (21), pp. 4155-4165. Date of Electronic Publication: 2021 Oct 26.
Typ publikacji :
Journal Article
MeSH Terms :
Cell Culture Techniques*
Spheroids, Cellular*
Biological Assay ; Cell Line, Tumor ; High-Throughput Screening Assays
Czasopismo naukowe
Tytuł :
Microfluidic arrays of dermal spheroids: a screening platform for active ingredients of skincare products.
Autorzy :
Chen Z; Department of Chemistry, University of Toronto, 80 Saint George Street, Toronto, Ontario, M5S 3H6, Canada. .
Kheiri S; Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, Ontario, M5S 3G8, Canada.
Gevorkian A; Department of Chemistry, University of Toronto, 80 Saint George Street, Toronto, Ontario, M5S 3H6, Canada. .
Young EWK; Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, Ontario, M5S 3G8, Canada.; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, M5S 3G9, Canada.
Andre V; BASF Beauty Care Solutions France S.A.S, 32, rue Saint Jean de Dieu, 69007, Lyon, France.
Deisenroth T; BASF Advanced Formulation Research North America, 500 White Plains Road, Tarrytown, New York, 10591, USA.
Kumacheva E; Department of Chemistry, University of Toronto, 80 Saint George Street, Toronto, Ontario, M5S 3H6, Canada. .; Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, Ontario, M5S 3E5, Canada.; Institute of Biomedical Engineering, University of Toronto, 164 College Street, Toronto, Ontario, M5S 3G9, Canada.
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Źródło :
Lab on a chip [Lab Chip] 2021 Oct 12; Vol. 21 (20), pp. 3952-3962. Date of Electronic Publication: 2021 Oct 12.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Culture Techniques*
Microfluidics*
Animals ; Hydrogels ; Spheroids, Cellular
Czasopismo naukowe
Tytuł :
Possibility of culturing the early developing kidney cells by utilizing simulated microgravity environment.
Autorzy :
Nishimura Y; Course of Clinical Engineering, Kitasato Junior College of Health Hygienic Sciences, Niigata, Japan. Electronic address: .
Wang PC; Division of Bioindustrial Science, Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki, Japan.
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Źródło :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2021 Oct 08; Vol. 573, pp. 9-12. Date of Electronic Publication: 2021 Aug 07.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Culture Techniques*
Weightlessness Simulation*
Kidney/*cytology
Animals ; Cell Differentiation ; Cells, Cultured ; Endothelial Cells/cytology ; Epithelial Cells/cytology ; Female ; Male ; Mice ; Mice, Inbred ICR
Czasopismo naukowe
Tytuł :
Chemical speciation of trace metals in mammalian cell culture media: looking under the hood to boost cellular performance and product quality.
Autorzy :
Stone AT; Department of Environmental Health and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Dhara VG; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Naik HM; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Aliyu L; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Lai J; Department of Environmental Health and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Jenkins J; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Betenbaugh MJ; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD 21218, USA. Electronic address: .
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Źródło :
Current opinion in biotechnology [Curr Opin Biotechnol] 2021 Oct; Vol. 71, pp. 216-224. Date of Electronic Publication: 2021 Aug 31.
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Review
MeSH Terms :
Cell Culture Techniques*
Metals*
Animals ; Biological Availability ; Ions ; Kinetics
Czasopismo naukowe
Tytuł :
Novel transfection methods in primary lung cell cultures.
Autorzy :
Kryvenko V; Department of Internal Medicine, Justus Liebig University, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research (DZL), Giessen, Germany.; The Cardio-Pulmonary Institute, Giessen, Germany.
Vadász I; Department of Internal Medicine, Justus Liebig University, Universities of Giessen and Marburg Lung Center, Member of the German Center for Lung Research (DZL), Giessen, Germany.; The Cardio-Pulmonary Institute, Giessen, Germany.; Institute for Lung Health, Giessen, Germany.
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Źródło :
American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2021 Oct 01; Vol. 321 (4), pp. L734-L735. Date of Electronic Publication: 2021 Sep 22.
Typ publikacji :
Editorial; Research Support, Non-U.S. Gov't; Comment
MeSH Terms :
Cell Culture Techniques*
Lung*
Transfection
Raport
Tytuł :
An efficient cell culture system for the studies of heterogeneous astrocytes: Time gradient digestion.
Autorzy :
Li Y; Department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Zhou P; Laboratory of Medical Systems Biology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623 Guangzhou, China. Electronic address: .
Zheng S; Laboratory of Medical Systems Biology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623 Guangzhou, China.
Liao C; Laboratory of Medical Systems Biology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623 Guangzhou, China.
Yang M; Laboratory of Medical Systems Biology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623 Guangzhou, China.
Gao S; Department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Wang X; Department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Chen Y; Department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Sun C; Department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Luo R; Department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
Huang Y; Guangzhou First People's Hospital, 510623 Guangzhou, China.
Li N; Laboratory of Medical Systems Biology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623 Guangzhou, China.
Hu H; Laboratory of Medical Systems Biology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623 Guangzhou, China; Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510623 Guangzhou, China; Third Affiliated Hospital of Zhengzhou University, 450052 Zhengzhou, China; School of Medicine, South China University of Technology, 510006 Guangzhou, China. Electronic address: .
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Źródło :
Journal of neuroscience methods [J Neurosci Methods] 2021 Oct 01; Vol. 362, pp. 109292. Date of Electronic Publication: 2021 Jul 22.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Astrocytes*
Cell Culture Techniques*
Animals ; Digestion ; Glial Fibrillary Acidic Protein ; Mice ; Mice, Inbred C57BL
Czasopismo naukowe
Tytuł :
Optimization of lymphapheresis for manufacturing autologous CAR-T cells.
Autorzy :
Yamanaka I; Center for Cellular and Molecular Medicine, Kyushu University Hospital, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Yamauchi T; Center for Cellular and Molecular Medicine, Kyushu University Hospital, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Henzan T; Center for Cellular and Molecular Medicine, Kyushu University Hospital, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Sakoda T; Center for Cellular and Molecular Medicine, Kyushu University Hospital, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Miyamoto K; Center for Cellular and Molecular Medicine, Kyushu University Hospital, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Mishima H; Department of Medical Technology, Kyushu University Hospital, Fukuoka, 812-8582, Japan.
Ono H; Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
Koga Y; Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
Nakashima Y; Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
Kato K; Department of Medicine and Biosystemic Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
Miyamoto T; Department of Medicine and Biosystemic Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
Mizuno S; Molecular and Cell Processing Center, Kyushu University Hospital, Fukuoka, 812-8582, Japan.
Ogawa Y; Department of Medicine and Bioregulatory Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
Ohga S; Department of Pediatrics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
Akashi K; Department of Medicine and Biosystemic Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
Maeda T; Center for Cellular and Molecular Medicine, Kyushu University Hospital, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.; Division of Precision Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
Kunisaki Y; Center for Cellular and Molecular Medicine, Kyushu University Hospital, 3-1-1, Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan. .
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Źródło :
International journal of hematology [Int J Hematol] 2021 Oct; Vol. 114 (4), pp. 449-458. Date of Electronic Publication: 2021 Jul 17.
Typ publikacji :
Journal Article
MeSH Terms :
Batch Cell Culture Techniques*/methods
Immunotherapy, Adoptive*
Receptors, Chimeric Antigen/*immunology
Receptors, Chimeric Antigen/*metabolism
T-Lymphocytes/*immunology
T-Lymphocytes/*metabolism
Adolescent ; Adult ; Aged ; Biomarkers ; Child ; Child, Preschool ; Female ; Humans ; Immunophenotyping ; Infant ; Leukapheresis/methods ; Male ; Middle Aged ; T-Lymphocytes/cytology ; Young Adult
Czasopismo naukowe
Tytuł :
Taking the pulse of bioprocesses: at-line and in-line monitoring of mammalian cell cultures.
Autorzy :
Fung Shek C; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, United States; Pivotal Bioprocess Sciences and Technologies, Amgen, 360 Binney Street, Cambridge, MA 02141, United States. Electronic address: .
Betenbaugh M; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, United States.
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Źródło :
Current opinion in biotechnology [Curr Opin Biotechnol] 2021 Oct; Vol. 71, pp. 191-197. Date of Electronic Publication: 2021 Aug 25.
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Review
MeSH Terms :
Bioreactors*
Cell Culture Techniques*
Animals ; Mammals
Czasopismo naukowe
Tytuł :
A liquid culture cancer spheroid model reveals low PI3K/Akt pathway activity and low adhesiveness to the extracellular matrix.
Autorzy :
Abe-Fukasawa N; Biological Research Laboratories, Nissan Chemical Corporation, Saitama, Japan.
Watanabe R; Biological Research Laboratories, Nissan Chemical Corporation, Saitama, Japan.
Gen Y; Faculty of Health Sciences, University of Bristol, UK.
Nishino T; Biological Research Laboratories, Nissan Chemical Corporation, Saitama, Japan.
Itasaki N; Faculty of Health Sciences, University of Bristol, UK.
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Źródło :
The FEBS journal [FEBS J] 2021 Oct; Vol. 288 (19), pp. 5650-5667. Date of Electronic Publication: 2021 May 02.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Culture Techniques*
Cell Adhesion/*genetics
Neoplasms/*genetics
Spheroids, Cellular/*pathology
Animals ; Cell Line, Tumor ; Cell Polarity/genetics ; Extracellular Matrix/genetics ; Humans ; Mice ; Neoplasm Metastasis ; Neoplasms/pathology ; Oncogene Protein v-akt/genetics ; Phosphatidylinositol 3-Kinases/genetics ; Signal Transduction/genetics ; Spheroids, Cellular/metabolism ; Transplantation, Heterologous
Czasopismo naukowe
Tytuł :
Towards integrated production of an influenza A vaccine candidate with MDCK suspension cells.
Autorzy :
Bissinger T; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
Wu Y; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
Marichal-Gallardo P; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
Riedel D; Facility for Transmission Electron Microscopy, Max Planck Institute for Biophysical Chemistry, Goettingen, Germany.
Liu X; Shanghai BioEngine Sci-Tech Co., Shanghai, China.
Genzel Y; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.
Tan WS; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.; Shanghai BioEngine Sci-Tech Co., Shanghai, China.
Reichl U; Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany.; Chair of Bioprocess Engineering, Otto von Guericke University Magdeburg, Magdeburg, Germany.
Pokaż więcej
Źródło :
Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Oct; Vol. 118 (10), pp. 3996-4013. Date of Electronic Publication: 2021 Jul 20.
Typ publikacji :
Journal Article
MeSH Terms :
COVID-19*
Cell Culture Techniques*
Influenza A Virus, H1N1 Subtype/*growth & development
Influenza Vaccines/*metabolism
SARS-CoV-2/*growth & development
Animals ; Dogs ; Madin Darby Canine Kidney Cells
Czasopismo naukowe

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