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Wyszukujesz frazę ""Cell Culture Techniques"" wg kryterium: Temat


Tytuł:
A long-awaited discovery: hypoxia prevents mouse cells from undergoing spontaneous p53-dependent transformation.
Autorzy:
Prockop DJ
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Źródło:
Cytotherapy [Cytotherapy] 2012 Oct; Vol. 14 (9), pp. 1029-31. Date of Electronic Publication: 2012 Aug 22.
Typ publikacji:
Journal Article
MeSH Terms:
Cell Culture Techniques*
Cell Hypoxia*
Cell Proliferation/*drug effects
Oxygen/*pharmacology
Tumor Suppressor Protein p53/*metabolism
Animals ; Cell Survival ; Cell Transformation, Neoplastic/drug effects ; Mice ; Mitochondria/metabolism ; Oxygen/metabolism ; Reactive Oxygen Species/metabolism
Czasopismo naukowe
Tytuł:
Intercellular cytosolic transfer correlates with mesenchymal stromal cell rescue of umbilical cord blood cell viability during ex vivo expansion.
Autorzy:
Chu PP; Department of Hematology, Singapore General Hospital, Singapore.
Bari S
Fan X
Gay FP
Ang JM
Chiu GN
Lim SK
Hwang WY
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Źródło:
Cytotherapy [Cytotherapy] 2012 Oct; Vol. 14 (9), pp. 1064-79. Date of Electronic Publication: 2012 Jul 10.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cell Culture Techniques*
Cytosol/*metabolism
Fetal Blood/*cytology
Leukocytes, Mononuclear/*cytology
Mesenchymal Stem Cells/*cytology
Animals ; Cell Death/genetics ; Cell Fusion ; Cell Proliferation ; Cell Survival ; Cell Transdifferentiation ; Coculture Techniques ; Green Fluorescent Proteins/analysis ; Humans ; Membrane Potential, Mitochondrial ; Mice ; NIH 3T3 Cells
Czasopismo naukowe
Tytuł:
The aggregate nature of human mesenchymal stromal cells in native bone marrow.
Autorzy:
Ahmadbeigi N; Department of Hematology, Tarbiat Modares University, Tehran, Iran.
Soleimani M
Babaeijandaghi F
Mortazavi Y
Gheisari Y
Vasei M
Azadmanesh K
Rostami S
Shafiee A
Nardi NB
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Źródło:
Cytotherapy [Cytotherapy] 2012 Sep; Vol. 14 (8), pp. 917-24. Date of Electronic Publication: 2012 Jun 11.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cell Culture Techniques*
Bone Marrow Cells/*cytology
Mesenchymal Stem Cells/*cytology
Cell Differentiation ; Culture Media ; Humans ; Leukocytes, Mononuclear/cytology
Czasopismo naukowe
Tytuł:
Effect of culture media on expansion properties of human umbilical cord matrix-derived mesenchymal cells.
Autorzy:
Salehinejad P; Institute of Bioscience, University Putra Malaysia, Kuala Lumpur, Malaysia.
Alitheen NB
Nematollahi-Mahani SN
Ali AM
Omar AR
Janzamin E
Hajghani M
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Źródło:
Cytotherapy [Cytotherapy] 2012 Sep; Vol. 14 (8), pp. 948-53. Date of Electronic Publication: 2012 May 15.
Typ publikacji:
Journal Article
MeSH Terms:
Cell Culture Techniques*
Mesenchymal Stem Cells/*cytology
Umbilical Cord/*cytology
Biomarkers ; Cell Differentiation ; Cell Proliferation ; Humans ; Organic Chemicals
Czasopismo naukowe
Tytuł:
Oxygen tension modifies the 'stemness' of human cord blood-derived stem cells.
Autorzy:
Buchheiser A; Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University Medical Center, Düsseldorf, Germany. />Houben AP
Bosch J
Marbach J
Liedtke S
Kögler G
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Źródło:
Cytotherapy [Cytotherapy] 2012 Sep; Vol. 14 (8), pp. 967-82. Date of Electronic Publication: 2012 Apr 12.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cell Culture Techniques*
Fetal Blood/*cytology
Mesenchymal Stem Cells/*cytology
Oxygen/*pharmacology
Adipogenesis/drug effects ; Adult ; Calcium-Binding Proteins ; Cell Differentiation/drug effects ; Cell Proliferation/drug effects ; Cell- and Tissue-Based Therapy ; Fetal Blood/metabolism ; Gene Expression Regulation/drug effects ; Genes, Homeobox/genetics ; Humans ; Intercellular Signaling Peptides and Proteins/metabolism ; Male ; Membrane Proteins/metabolism ; Mesenchymal Stem Cells/metabolism ; Middle Aged ; Osteogenesis/drug effects ; Telomere Shortening ; Wnt Proteins/metabolism
Czasopismo naukowe
Tytuł:
Development of a surrogate angiogenic potency assay for clinical-grade stem cell production.
Autorzy:
Lehman N; Athersys Inc., Regenerative Medicine Program, Cleveland, Ohio 44115, USA.
Cutrone R
Raber A
Perry R
Van't Hof W
Deans R
Ting AE
Woda J
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Źródło:
Cytotherapy [Cytotherapy] 2012 Sep; Vol. 14 (8), pp. 994-1004. Date of Electronic Publication: 2012 Jun 11.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cell Culture Techniques*
Cell- and Tissue-Based Therapy*
Neovascularization, Physiologic*
Endothelial Cells/*cytology
Multipotent Stem Cells/*cytology
Bone Marrow Cells/cytology ; Cell Differentiation ; Culture Media, Conditioned ; Culture Media, Serum-Free ; Cytokines/metabolism ; Gene Expression ; Humans ; Interleukin-8/metabolism ; Multipotent Stem Cells/transplantation ; Myocardial Infarction/therapy ; Receptors, G-Protein-Coupled/metabolism ; Vascular Endothelial Growth Factor A/metabolism
Czasopismo naukowe
Tytuł:
Cord blood culture in hypoxia: making the cells feel at home.
Autorzy:
Larochelle A; National Institutes of Health, National Heart, Lung, and Blood Institute, Hematology Branch, Bethesda, MD 20892, USA.
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Źródło:
Cytotherapy [Cytotherapy] 2012 Sep; Vol. 14 (8), pp. 900-1.
Typ publikacji:
Journal Article; Comment
MeSH Terms:
Cell Culture Techniques*
Fetal Blood/*cytology
Mesenchymal Stem Cells/*cytology
Oxygen/*pharmacology
Humans ; Male
Czasopismo naukowe
Tytuł:
Development and validation of a multiplex quantitative polymerase chain reaction assay for the detection of Mollicutes impurities in human cells, cultured under good manufacturing practice conditions, and following European Pharmacopoeia requirements and the International Conference on Harmonization guidelines.
Autorzy:
Vanni I; Molecular Medicine, Istituto Giannina Gaslini, Genova, Italy.
Ugolotti E
Raso A
Di Marco E
Melioli G
Biassoni R
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Źródło:
Cytotherapy [Cytotherapy] 2012 Jul; Vol. 14 (6), pp. 752-66. Date of Electronic Publication: 2012 Mar 29.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms:
DNA Contamination*
Guidelines as Topic*
Pharmacopoeias as Topic*
Cell Culture Techniques/*standards
Multiplex Polymerase Chain Reaction/*methods
Multiplex Polymerase Chain Reaction/*standards
Tenericutes/*isolation & purification
Adult ; Base Sequence ; Biological Assay ; Cell Culture Techniques/methods ; Cells, Cultured ; DNA Primers/metabolism ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Europe ; Humans ; Limit of Detection ; Molecular Sequence Data ; Reference Standards ; Reproducibility of Results ; Sensitivity and Specificity ; Sequence Alignment ; Tenericutes/genetics
Czasopismo naukowe
Tytuł:
Replicative aging and differentiation potential of human adipose tissue-derived mesenchymal stromal cells expanded in pooled human or fetal bovine serum.
Autorzy:
Bieback K; Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, German Red Cross Blood Service Baden-Württemberg - Hessen, Germany. />Hecker A
Schlechter T
Hofmann I
Brousos N
Redmer T
Besser D
Klüter H
Müller AM
Becker M
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Źródło:
Cytotherapy [Cytotherapy] 2012 May; Vol. 14 (5), pp. 570-83. Date of Electronic Publication: 2012 Feb 02.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cell Culture Techniques*
Culture Media*/pharmacology
Serum*
Adipose Tissue/*cytology
Mesenchymal Stem Cells/*cytology
Adipose Tissue/metabolism ; Adult ; Animals ; Cattle ; Cell Differentiation/drug effects ; Cell Proliferation/drug effects ; Cells, Cultured ; Female ; Fibroblasts/cytology ; Humans ; Middle Aged
Czasopismo naukowe
Tytuł:
Characterization of adipose tissue-derived cells isolated with the Celution system.
Autorzy:
Lin K; Corporate R&D Center, Olympus Corporation, Olympus Corporation, Kobe International Business Center 607, Hachioji-shi, Tokyo, Japan. konghua_ />Matsubara Y
Masuda Y
Togashi K
Ohno T
Tamura T
Toyoshima Y
Sugimachi K
Toyoda M
Marc H
Douglas A
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Źródło:
Cytotherapy [Cytotherapy] 2008; Vol. 10 (4), pp. 417-26.
Typ publikacji:
Evaluation Study; Journal Article
MeSH Terms:
Cell Culture Techniques*/instrumentation
Cell Culture Techniques*/methods
Adipocytes/*cytology
Adipose Tissue/*cytology
Stem Cells/*cytology
Adipogenesis ; Animals ; Antigens, CD/metabolism ; Biomarkers/metabolism ; Cell Differentiation ; Cells, Cultured ; Flow Cytometry ; Humans ; Materials Testing ; Osteogenesis
Czasopismo naukowe
Tytuł:
Production of good manufacturing practice-grade cytotoxic T lymphocytes specific for Epstein-Barr virus, cytomegalovirus and adenovirus to prevent or treat viral infections post-allogeneic hematopoietic stem cell transplant.
Autorzy:
Sili U; Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children's Hospital, The Methodist Hospital, Houston, Texas 77030, USA.
Leen AM
Vera JF
Gee AP
Huls H
Heslop HE
Bollard CM
Rooney CM
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Źródło:
Cytotherapy [Cytotherapy] 2012 Jan; Vol. 14 (1), pp. 7-11.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms:
Cell Culture Techniques*
Adenoviridae/*immunology
Cytomegalovirus/*immunology
Hematopoietic Stem Cell Transplantation/*adverse effects
Herpesvirus 4, Human/*immunology
T-Lymphocytes, Cytotoxic/*immunology
Virus Diseases/*etiology
Antigen-Presenting Cells/immunology ; Antigen-Presenting Cells/transplantation ; Cell Line, Transformed ; Cell Proliferation ; Cytotoxicity, Immunologic ; HLA Antigens/immunology ; Humans ; Phosphoproteins/immunology ; T-Lymphocytes, Cytotoxic/transplantation ; Transplantation, Homologous/adverse effects ; Viral Matrix Proteins/immunology ; Virus Diseases/therapy
Czasopismo naukowe
Tytuł:
Epidermal growth factor, basic fibroblast growth factor and platelet-derived growth factor-bb can substitute for fetal bovine serum and compete with human platelet-rich plasma in the ex vivo expansion of mesenchymal stromal cells derived from adipose tissue.
Autorzy:
Chieregato K; Department of Cell Therapy and Hematology, San Bortolo Hospital, Vicenza, Italy.
Castegnaro S
Madeo D
Astori G
Pegoraro M
Rodeghiero F
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Źródło:
Cytotherapy [Cytotherapy] 2011 Sep; Vol. 13 (8), pp. 933-43. Date of Electronic Publication: 2011 May 30.
Typ publikacji:
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cell Culture Techniques*
Regenerative Medicine*/trends
Adipose Tissue/*cytology
Mesenchymal Stem Cells/*metabolism
Platelet-Rich Plasma/*metabolism
Adipogenesis/drug effects ; Animals ; Antigens, CD/metabolism ; Cattle ; Cell Proliferation/drug effects ; Cells, Cultured ; Epidermal Growth Factor/pharmacology ; Fibroblast Growth Factor 2/pharmacology ; Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology ; Humans ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/drug effects ; Osteogenesis/drug effects ; Platelet-Derived Growth Factor/pharmacology
Czasopismo naukowe
Tytuł:
Use of animal protein-free products for passaging adherent human adipose-derived stromal/stem cells.
Autorzy:
Carvalho PP; Stem Cell Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, Louisiana 70808, USA.
Wu X
Yu G
Dietrich M
Dias IR
Gomes ME
Reis RL
Gimble JM
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Źródło:
Cytotherapy [Cytotherapy] 2011 May; Vol. 13 (5), pp. 594-7. Date of Electronic Publication: 2011 Jan 03.
Typ publikacji:
Comparative Study; Journal Article
MeSH Terms:
Cell Culture Techniques*
Cell Separation*
Adipose Tissue/*cytology
Mesenchymal Stem Cells/*cytology
Recombinant Proteins/*chemistry
Trypsin/*chemistry
Adipose Tissue/physiology ; Adult ; Animals ; Cell Count ; Cell Differentiation ; Cell Survival ; Cells, Cultured ; Culture Media ; Female ; Humans ; Immunophenotyping ; Mesenchymal Stem Cells/physiology ; Middle Aged ; Stromal Cells/cytology ; Stromal Cells/physiology ; Swine ; Young Adult
Czasopismo naukowe
Tytuł:
Effect of cell-seeding density on the proliferation and gene expression profile of human umbilical vein endothelial cells within ex vivo culture.
Autorzy:
Heng BC; School of Materials Science and Engineering, Nanyang Technological University, Singapore. />Bezerra PP
Preiser PR
Law SK
Xia Y
Boey F
Venkatraman SS
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Źródło:
Cytotherapy [Cytotherapy] 2011 May; Vol. 13 (5), pp. 606-17. Date of Electronic Publication: 2010 Dec 20.
Typ publikacji:
Journal Article
MeSH Terms:
Cell Culture Techniques*
Endothelium, Vascular/*cytology
Endothelium, Vascular/*metabolism
Umbilical Veins/*cytology
Cell Count ; Cell Proliferation ; Gelatin/metabolism ; Gene Expression Profiling ; Humans ; Lactic Acid/metabolism ; Polyesters ; Polymers/metabolism ; Polystyrenes/metabolism
Czasopismo naukowe
Tytuł:
Multicenter cell processing for cardiovascular regenerative medicine applications: the Cardiovascular Cell Therapy Research Network (CCTRN) experience.
Autorzy:
Gee AP; Center for Cell and Gene Therapy, Baylor College of Medicine, MC3-3320, Feigin Center, 102 Bates Street, Houston, TX 77030, USA.
Richman S
Durett A
McKenna D
Traverse J
Henry T
Fisk D
Pepine C
Bloom J
Willerson J
Prater K
Zhao D
Koç JR
Ellis S
Taylor D
Cogle C
Moyé L
Simari R
Skarlatos S
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Źródło:
Cytotherapy [Cytotherapy] 2010 Sep; Vol. 12 (5), pp. 684-91.
Typ publikacji:
Clinical Trial, Phase II; Journal Article; Multicenter Study; Randomized Controlled Trial; Research Support, N.I.H., Extramural
MeSH Terms:
Cell Culture Techniques/*standards
Cell- and Tissue-Based Therapy/*standards
Mesenchymal Stem Cells/*metabolism
Myocardial Infarction/*therapy
Automation, Laboratory ; Bone Marrow/pathology ; Cell Count ; Cell Culture Techniques/methods ; Cell Separation/methods ; Cell- and Tissue-Based Therapy/economics ; Flow Cytometry ; Humans ; Laboratory Proficiency Testing ; Mesenchymal Stem Cells/pathology ; Myocardial Infarction/pathology ; Myocardial Infarction/physiopathology ; Practice Guidelines as Topic ; Quality Control ; Regenerative Medicine/methods ; Validation Studies as Topic
Czasopismo naukowe
Tytuł:
A simplified culture and polymerase chain reaction identification assay for quality control performance testing of stem cell media products.
Autorzy:
Boucher S; Life Technologies, Primary and Stem Cell Systems, Frederick, Maryland 21704,USA. />Lakshmipathy U
Vemuri M
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Źródło:
Cytotherapy [Cytotherapy] 2009; Vol. 11 (6), pp. 761-7, 767.e1-2.
Typ publikacji:
Evaluation Study; Journal Article
MeSH Terms:
Cell Culture Techniques*
Adipogenesis/*genetics
Mesenchymal Stem Cells/*physiology
Oligonucleotide Array Sequence Analysis/*methods
Polymerase Chain Reaction/*methods
Culture Media/analysis ; Fatty Acid-Binding Proteins/genetics ; Gene Expression ; Humans ; Quality Control ; Up-Regulation
Czasopismo naukowe
Tytuł:
Optimization and validation of a robust human T-cell culture method for monitoring phenotypic and polyfunctional antigen-specific CD4 and CD8 T-cell responses.
Autorzy:
Lin Y; Ludwig Center for Cancer Immunotherapy, Immunology Program, Sloan-Kettering Institute, Newy York, New York 10021, USA.
Gallardo HF
Ku GY
Li H
Manukian G
Rasalan TS
Xu Y
Terzulli SL
Old LJ
Allison JP
Houghton AN
Wolchok JD
Yuan J
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Źródło:
Cytotherapy [Cytotherapy] 2009; Vol. 11 (7), pp. 912-22.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cell Culture Techniques*
CD4-Positive T-Lymphocytes/*metabolism
CD8-Positive T-Lymphocytes/*metabolism
Neoplasms/*immunology
Antigens/immunology ; Antigens, CD/metabolism ; Antigens, Differentiation/metabolism ; CD4-Positive T-Lymphocytes/immunology ; CD4-Positive T-Lymphocytes/pathology ; CD8-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/pathology ; Cancer Vaccines ; Cells, Cultured ; Cytokines/metabolism ; Feasibility Studies ; Humans ; Neoplasms/therapy
Czasopismo naukowe
Tytuł:
Heterogeneity of mesenchymal stromal cell preparations.
Autorzy:
Ho AD; Department of Medicine V, University of Heidelberg, Heidelberg, Germany. Anthony_ />Wagner W
Franke W
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Źródło:
Cytotherapy [Cytotherapy] 2008; Vol. 10 (4), pp. 320-30.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms:
Cell Culture Techniques*
Coculture Techniques*
Mesenchymal Stem Cells*/cytology
Mesenchymal Stem Cells*/physiology
Stromal Cells*/cytology
Stromal Cells*/physiology
Animals ; Biomarkers/metabolism ; Cell Differentiation ; Cell Lineage ; Cell Separation ; Cells, Cultured ; Humans ; Intercellular Junctions/metabolism ; Phenotype
Czasopismo naukowe
Tytuł:
Retronectin-assisted retroviral transduction of primary human T lymphocytes under good manufacturing practice conditions: tissue culture bag critically determines cell yield.
Autorzy:
Lamers CH; Laboratory of Clinical and Tumor Immunology, Erasmus MC-Daniel den Hoed Cancer Center, Rotterdam, The Netherlands. />van Elzakker P
van Steenbergen SC
Sleijfer S
Debets R
Gratama JW
Pokaż więcej
Źródło:
Cytotherapy [Cytotherapy] 2008; Vol. 10 (4), pp. 406-16.
Typ publikacji:
Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cell Culture Techniques/*instrumentation
Retroviridae/*metabolism
T-Lymphocytes/*physiology
Transduction, Genetic/*methods
Animals ; Carcinoma, Renal Cell/immunology ; Carcinoma, Renal Cell/therapy ; Cell Culture Techniques/methods ; Cells, Cultured ; Cytotoxicity, Immunologic ; Humans ; Immunotherapy, Adoptive/methods ; Materials Testing ; Retroviridae/genetics ; T-Lymphocytes/cytology
Czasopismo naukowe

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