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Tytuł :
Solution NMR structure and functional analysis of the integral membrane protein YgaP from Escherichia coli.
Autorzy :
Eichmann C; From the Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland and.
Tzitzilonis C; From the Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland and the Structural Biology Laboratory, The Salk Institute, La Jolla, California 92037.
Bordignon E; From the Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland and.
Maslennikov I; the Structural Biology Laboratory, The Salk Institute, La Jolla, California 92037.
Choe S; the Structural Biology Laboratory, The Salk Institute, La Jolla, California 92037.
Riek R; From the Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland and the Structural Biology Laboratory, The Salk Institute, La Jolla, California 92037 .
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Źródło :
The Journal of biological chemistry [J Biol Chem] 2014 Aug 22; Vol. 289 (34), pp. 23482-503. Date of Electronic Publication: 2014 Jun 23.
Typ publikacji :
Journal Article
MeSH Terms :
Escherichia coli/*chemistry
Escherichia coli Proteins/*chemistry
Escherichia coli Proteins/*physiology
Membrane Proteins/*chemistry
Membrane Proteins/*physiology
Nuclear Magnetic Resonance, Biomolecular/*methods
Models, Molecular ; Protein Conformation ; Structure-Activity Relationship ; Thiosulfate Sulfurtransferase/chemistry
Czasopismo naukowe
Tytuł :
Drug screening strategy for human membrane proteins: from NMR protein backbone structure to in silica- and NMR-screened hits.
Autorzy :
Lindert S; Department of Chemistry and Biochemistry, Department of Pharmacology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Maslennikov I; Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Rd., La Jolla, CA 92037, USA; Joint Center for Biosciences, 301-B, Songdo Smart Valley 214, Songdo-dong, Yeonsu-ku, Incheon 406-840, Republic of Korea.
Chiu EJ; Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Rd., La Jolla, CA 92037, USA.
Pierce LC; Department of Chemistry and Biochemistry, Department of Pharmacology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
McCammon JA; Department of Chemistry and Biochemistry, Department of Pharmacology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Howard Hughes Medical Institute, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; NSF Center for Theoretical Biological Physics, National Biomedical Computation Resource, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Choe S; Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Rd., La Jolla, CA 92037, USA; Joint Center for Biosciences, 301-B, Songdo Smart Valley 214, Songdo-dong, Yeonsu-ku, Incheon 406-840, Republic of Korea; Drug Discovery Collaboratory, Carlsbad, CA 92008, USA. Electronic address: choe@salk.edu.
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Źródło :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2014 Mar 21; Vol. 445 (4), pp. 724-33. Date of Electronic Publication: 2014 Feb 10.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Drug Design*
Membrane Proteins/*chemistry
Neoplasm Proteins/*chemistry
Nuclear Magnetic Resonance, Biomolecular/*methods
Binding Sites ; Humans ; Intracellular Signaling Peptides and Proteins ; Ligands ; Membrane Proteins/metabolism ; Mitochondrial Proteins ; Molecular Docking Simulation ; Neoplasm Proteins/metabolism ; Protein Conformation
Czasopismo naukowe
Tytuł :
Advances in NMR structures of integral membrane proteins.
Autorzy :
Maslennikov I; Structural Biology Laboratory, Salk Institute, La Jolla, CA 92037, USA.
Choe S
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Źródło :
Current opinion in structural biology [Curr Opin Struct Biol] 2013 Aug; Vol. 23 (4), pp. 555-62. Date of Electronic Publication: 2013 May 27.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Membrane Proteins/*ultrastructure
Nuclear Magnetic Resonance, Biomolecular/*methods
Cell Communication ; Humans ; Membrane Proteins/metabolism ; Protein Structure, Tertiary ; Signal Transduction
Czasopismo naukowe
Tytuł :
Detergent/nanodisc screening for high-resolution NMR studies of an integral membrane protein containing a cytoplasmic domain.
Autorzy :
Tzitzilonis C; Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, ETH-Hönggerberg, Zürich, Switzerland.
Eichmann C
Maslennikov I
Choe S
Riek R
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Źródło :
PloS one [PLoS One] 2013; Vol. 8 (1), pp. e54378. Date of Electronic Publication: 2013 Jan 22.
Typ publikacji :
Journal Article
MeSH Terms :
Detergents/*chemistry
Escherichia coli Proteins/*chemistry
Membrane Proteins/*chemistry
Nanostructures/*chemistry
Biomimetics ; Crystallography, X-Ray ; Cytoplasm/chemistry ; Magnetic Resonance Spectroscopy ; Membrane Proteins/analysis ; Protein Structure, Secondary ; Protein Structure, Tertiary
Czasopismo naukowe
Tytuł :
Facile backbone structure determination of human membrane proteins by NMR spectroscopy.
Autorzy :
Klammt C; Structural Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, USA.
Maslennikov I
Bayrhuber M
Eichmann C
Vajpai N
Chiu EJ
Blain KY
Esquivies L
Kwon JH
Balana B
Pieper U
Sali A
Slesinger PA
Kwiatkowski W
Riek R
Choe S
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Źródło :
Nature methods [Nat Methods] 2012 May 20; Vol. 9 (8), pp. 834-9. Date of Electronic Publication: 2012 May 20.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Membrane Proteins/*chemistry
Nuclear Magnetic Resonance, Biomolecular/*methods
Databases, Protein ; Humans ; Models, Molecular ; Molecular Weight ; Protein Conformation
Czasopismo naukowe
Tytuł :
Mechanism underlying selective regulation of G protein-gated inwardly rectifying potassium channels by the psychostimulant-sensitive sorting nexin 27.
Autorzy :
Balana B; Clayton Foundation Laboratories for Peptide Biology, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Maslennikov I
Kwiatkowski W
Stern KM
Bahima L
Choe S
Slesinger PA
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Źródło :
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2011 Apr 05; Vol. 108 (14), pp. 5831-6. Date of Electronic Publication: 2011 Mar 21.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Models, Molecular*
G Protein-Coupled Inwardly-Rectifying Potassium Channels/*metabolism
Intracellular Signaling Peptides and Proteins/*metabolism
Membrane Proteins/*metabolism
Neurons/*physiology
Sorting Nexins/*metabolism
Amino Acid Motifs/physiology ; Animals ; COS Cells ; Chlorocebus aethiops ; Crystallization ; Disks Large Homolog 4 Protein ; Electrophysiology ; G Protein-Coupled Inwardly-Rectifying Potassium Channels/chemistry ; G Protein-Coupled Inwardly-Rectifying Potassium Channels/genetics ; Hippocampus/cytology ; Humans ; Image Processing, Computer-Assisted ; Immunohistochemistry ; Intracellular Signaling Peptides and Proteins/chemistry ; Intracellular Signaling Peptides and Proteins/genetics ; Membrane Proteins/chemistry ; Membrane Proteins/genetics ; Microscopy, Confocal ; Mutagenesis, Site-Directed ; Neurons/metabolism ; Protein Structure, Tertiary ; Rats
Czasopismo naukowe
Tytuł :
Rapid exploration of the folding topology of helical membrane proteins using paramagnetic perturbation.
Autorzy :
Yeo KJ; Division of Magnetic Resonance, Korea Basic Science Institute (KBSI), Yangcheong-Ri, Ochang, Chungbuk 363-883, Korea.
Kim HY
Kim YP
Hwang E
Kim MH
Cheong C
Choe S
Jeon YH
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Źródło :
Protein science : a publication of the Protein Society [Protein Sci] 2010 Dec; Vol. 19 (12), pp. 2409-17. Date of Electronic Publication: 2010 Nov 11.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Membrane Proteins/*chemistry
Magnetic Resonance Spectroscopy ; Membrane Proteins/metabolism ; Micelles ; Phosphorylcholine/analogs & derivatives ; Phosphorylcholine/chemistry ; Protein Folding ; Protein Structure, Secondary
Czasopismo naukowe
Tytuł :
Membrane domain structures of three classes of histidine kinase receptors by cell-free expression and rapid NMR analysis.
Autorzy :
Maslennikov I; Structural Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Klammt C
Hwang E
Kefala G
Okamura M
Esquivies L
Mörs K
Glaubitz C
Kwiatkowski W
Jeon YH
Choe S
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Źródło :
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2010 Jun 15; Vol. 107 (24), pp. 10902-7. Date of Electronic Publication: 2010 May 24.
Typ publikacji :
Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Validation Study
MeSH Terms :
Escherichia coli Proteins/*chemistry
Membrane Proteins/*chemistry
Protein Kinases/*chemistry
Amino Acid Sequence ; Bacteriological Techniques ; Carbon Isotopes ; Escherichia coli/chemistry ; Escherichia coli/genetics ; Escherichia coli Proteins/genetics ; Histidine Kinase ; Membrane Proteins/genetics ; Models, Molecular ; Molecular Sequence Data ; Multiprotein Complexes/chemistry ; Nitrogen Isotopes ; Nuclear Magnetic Resonance, Biomolecular ; Protein Kinases/genetics ; Protein Structure, Tertiary ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics
Czasopismo naukowe
Tytuł :
Bacterial expression of a eukaryotic membrane protein in fusion to various Mistic orthologs.
Autorzy :
Dvir H; Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
Choe S
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Źródło :
Protein expression and purification [Protein Expr Purif] 2009 Nov; Vol. 68 (1), pp. 28-33. Date of Electronic Publication: 2009 Jun 12.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Bacterial Proteins/*metabolism
Membrane Proteins/*metabolism
Potassium Channels/*metabolism
Recombinant Fusion Proteins/*metabolism
Amino Acid Sequence ; Bacillus/genetics ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Hydrogen-Ion Concentration ; Light ; Membrane Proteins/chemistry ; Membrane Proteins/genetics ; Models, Biological ; Molecular Sequence Data ; Potassium Channels/chemistry ; Potassium Channels/genetics ; Recombinant Fusion Proteins/chemistry ; Recombinant Fusion Proteins/genetics ; Scattering, Radiation ; Sequence Alignment
Czasopismo naukowe
Tytuł :
Mistic: cellular localization, solution behavior, polymerization, and fibril formation.
Autorzy :
Dvir H; Structural Biology Laboratory, The Salk Institute for Biological Studies, San Diego, California 92037, USA.
Lundberg ME
Maji SK
Riek R
Choe S
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Źródło :
Protein science : a publication of the Protein Society [Protein Sci] 2009 Jul; Vol. 18 (7), pp. 1564-70.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Bacillus subtilis/*metabolism
Bacterial Proteins/*chemistry
Membrane Proteins/*chemistry
Amino Acid Sequence ; Bacillus subtilis/genetics ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Circular Dichroism ; Crystallography, X-Ray ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Light ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Molecular Sequence Data ; Mutation ; Nuclear Magnetic Resonance, Biomolecular ; Protein Multimerization ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Scattering, Radiation ; Sequence Alignment ; Solubility
Czasopismo naukowe
Tytuł :
2007 annual progress report synopsis of the Center for Structures of Membrane Proteins.
Autorzy :
Stroud RM; The Center for Structures of Membrane Proteins (CSMP), UCSF, San Francisco, CA 94158-2517, USA. />Choe S
Holton J
Kaback HR
Kwiatkowski W
Minor DL
Riek R
Sali A
Stahlberg H
Harries W
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Źródło :
Journal of structural and functional genomics [J Struct Funct Genomics] 2009 Apr; Vol. 10 (2), pp. 193-208. Date of Electronic Publication: 2009 Jan 16.
Typ publikacji :
Journal Article
MeSH Terms :
Membrane Proteins/*chemistry
Proteomics/*methods
Membrane Proteins/metabolism ; Multi-Institutional Systems ; Protein Conformation ; Proteome/chemistry ; Proteome/metabolism ; Structure-Activity Relationship
Czasopismo naukowe
Tytuł :
Characterization of protein detergent complexes by NMR, light scattering, and analytical ultracentrifugation.
Autorzy :
Maslennikov I; Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Rd, La Jolla, CA 92037, USA.
Krupa M
Dickson C
Esquivies L
Blain K
Kefala G
Choe S
Kwiatkowski W
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Źródło :
Journal of structural and functional genomics [J Struct Funct Genomics] 2009 Mar; Vol. 10 (1), pp. 25-35. Date of Electronic Publication: 2009 Feb 12.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Detergents/*chemistry
Magnetic Resonance Spectroscopy/*methods
Membrane Proteins/*chemistry
Ultracentrifugation/*methods
Light ; Membrane Proteins/metabolism ; Scattering, Radiation ; Surface Properties
Czasopismo naukowe
Tytuł :
A continuum method for determining membrane protein insertion energies and the problem of charged residues.
Autorzy :
Choe S; Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA.
Hecht KA
Grabe M
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Źródło :
The Journal of general physiology [J Gen Physiol] 2008 Jun; Vol. 131 (6), pp. 563-73. Date of Electronic Publication: 2008 May 12.
Typ publikacji :
Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Static Electricity*
Biophysics/*methods
Membrane Proteins/*metabolism
Protein Processing, Post-Translational/*physiology
Cell Membrane/chemistry ; Cell Membrane/metabolism ; Computer Simulation ; Elasticity ; Energy Transfer/physiology ; Extracellular Space ; Hydrophobic and Hydrophilic Interactions ; Lipid Bilayers/chemistry ; Lipid Bilayers/metabolism ; Membrane Fluidity/physiology ; Membrane Proteins/chemistry ; Models, Chemical ; Models, Molecular ; Protein Conformation ; Protein Transport/physiology ; Thermodynamics
Czasopismo naukowe
Tytuł :
Neogenin interacts with hemojuvelin through its two membrane-proximal fibronectin type III domains.
Autorzy :
Yang F; Graduate Option in Chemistry, California Institute of Technology, Pasadena, California 91125, USA.
West AP Jr
Allendorph GP
Choe S
Bjorkman PJ
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Źródło :
Biochemistry [Biochemistry] 2008 Apr 08; Vol. 47 (14), pp. 4237-45. Date of Electronic Publication: 2008 Mar 13.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Cell Membrane/*metabolism
Fibronectins/*metabolism
Membrane Proteins/*metabolism
Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins/metabolism ; Fibronectins/chemistry ; Fibronectins/genetics ; GPI-Linked Proteins ; Hemochromatosis Protein ; Humans ; Membrane Proteins/genetics ; Membrane Proteins/isolation & purification ; Peptide Fragments/chemistry ; Peptide Fragments/metabolism ; Protein Binding ; Surface Plasmon Resonance ; Transforming Growth Factor beta/metabolism
Czasopismo naukowe
Tytuł :
Application of Mistic to improving the expression and membrane integration of histidine kinase receptors from Escherichia coli.
Autorzy :
Kefala G; Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Rd, La Jolla, CA 92037, USA.
Kwiatkowski W
Esquivies L
Maslennikov I
Choe S
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Źródło :
Journal of structural and functional genomics [J Struct Funct Genomics] 2007 Dec; Vol. 8 (4), pp. 167-72. Date of Electronic Publication: 2007 Nov 06.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Bacillus subtilis/*chemistry
Escherichia coli/*enzymology
Escherichia coli Proteins/*metabolism
Membrane Proteins/*metabolism
Protein Kinases/*metabolism
Receptors, Cell Surface/*metabolism
Recombinant Fusion Proteins/*metabolism
Blotting, Western ; Histidine Kinase ; Membrane Proteins/chemistry ; Membrane Proteins/genetics ; Phosphorylation ; Polymerase Chain Reaction ; Recombinant Fusion Proteins/genetics
Czasopismo naukowe
Tytuł :
NMR spectroscopic and analytical ultracentrifuge analysis of membrane protein detergent complexes.
Autorzy :
Maslennikov I; Structural Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Rd., La Jolla, CA 92037, USA. />Kefala G
Johnson C
Riek R
Choe S
Kwiatkowski W
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Źródło :
BMC structural biology [BMC Struct Biol] 2007 Nov 08; Vol. 7, pp. 74. Date of Electronic Publication: 2007 Nov 08.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
Detergents/*chemistry
Membrane Proteins/*chemistry
Cell Membrane/chemistry ; Crystallography, X-Ray ; Electrophoresis, Polyacrylamide Gel ; Gene Expression ; Magnetic Resonance Spectroscopy ; Membrane Proteins/genetics ; Reproducibility of Results ; Solubility ; Ultracentrifugation
Czasopismo naukowe
Tytuł :
NMR studies of interactions between C-terminal tail of Kir2.1 channel and PDZ1,2 domains of PSD95.
Autorzy :
Pegan S; Structural Biology, The Salk Institute, La Jolla, California 92037, USA.
Tan J
Huang A
Slesinger PA
Riek R
Choe S
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Źródło :
Biochemistry [Biochemistry] 2007 May 08; Vol. 46 (18), pp. 5315-22. Date of Electronic Publication: 2007 Apr 17.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms :
Adaptor Proteins, Signal Transducing/*metabolism
Carrier Proteins/*metabolism
Intracellular Signaling Peptides and Proteins/*metabolism
Membrane Proteins/*metabolism
Peptide Fragments/*metabolism
Potassium Channels, Inwardly Rectifying/*metabolism
Adaptor Proteins, Signal Transducing/chemistry ; Amino Acid Motifs ; Amino Acid Sequence ; Animals ; Carrier Proteins/chemistry ; Cytoplasm/chemistry ; Cytoplasm/metabolism ; Cytoskeletal Proteins ; Disks Large Homolog 4 Protein ; Humans ; Intracellular Signaling Peptides and Proteins/chemistry ; Membrane Proteins/chemistry ; Mice ; Molecular Sequence Data ; Nuclear Magnetic Resonance, Biomolecular ; Peptide Fragments/chemistry ; Potassium Channels, Inwardly Rectifying/chemistry ; Protein Binding ; Protein Structure, Secondary ; Protein Structure, Tertiary ; Rats ; Thermodynamics
Czasopismo naukowe
Tytuł :
Redesigning an integral membrane K+ channel into a soluble protein.
Autorzy :
Roosild TP; Structural Biology Laboratory, Salk Institute and Division of Biology, University of California at San Diego, San Diego, CA 92037, USA.
Choe S
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Źródło :
Protein engineering, design & selection : PEDS [Protein Eng Des Sel] 2005 Feb; Vol. 18 (2), pp. 79-84. Date of Electronic Publication: 2005 Mar 23.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.
MeSH Terms :
Membrane Proteins/*chemistry
Potassium Channels/*chemistry
Amino Acid Sequence ; Guanidine/chemistry ; Membrane Proteins/genetics ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Potassium Channels/genetics ; Sequence Homology, Amino Acid
Czasopismo naukowe

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