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Wyszukujesz frazę ""Cloning, Molecular"" wg kryterium: Temat


Tytuł :
Principles and Practical Considerations for the Analysis of Disease-Associated Alternative Splicing Events Using the Gateway Cloning-Based Minigene Vectors pDESTsplice and pSpliceExpress.
Autorzy :
Putscher E; Division of Neuroimmunology, Department of Neurology, Rostock University Medical Center, Gehlsheimer Street 20, 18147 Rostock, Germany.
Hecker M; Division of Neuroimmunology, Department of Neurology, Rostock University Medical Center, Gehlsheimer Street 20, 18147 Rostock, Germany.
Fitzner B; Division of Neuroimmunology, Department of Neurology, Rostock University Medical Center, Gehlsheimer Street 20, 18147 Rostock, Germany.
Lorenz P; Rostock University Medical Center, Institute of Immunology, Schillingallee 70, 18057 Rostock, Germany.
Zettl UK; Division of Neuroimmunology, Department of Neurology, Rostock University Medical Center, Gehlsheimer Street 20, 18147 Rostock, Germany.
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Źródło :
International journal of molecular sciences [Int J Mol Sci] 2021 May 13; Vol. 22 (10). Date of Electronic Publication: 2021 May 13.
Typ publikacji :
Journal Article; Review
MeSH Terms :
Alternative Splicing*
Cloning, Molecular*
Genes, Reporter*
Genome, Human*
Mutation*
Genetic Diseases, Inborn/*diagnosis
Genetic Vectors/*genetics
DNA Restriction Enzymes ; Genetic Diseases, Inborn/genetics ; Humans
Czasopismo naukowe
Tytuł :
Fast and efficient purification of SARS-CoV-2 RNA dependent RNA polymerase complex expressed in Escherichia coli.
Autorzy :
Madru C; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.
Tekpinar AD; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.; Department of Molecular Biology and Genetics, Faculty of Science, Van Yüzüncü Yıl University, Van, Turkey.
Rosario S; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.
Czernecki D; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.; École Doctorale Complexité du Vivant, Sorbonne Université, Paris, France.
Brûlé S; Molecular Biophysics Platform, C2RT, Institut Pasteur, CNRS UMR, Paris, France.
Sauguet L; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.
Delarue M; Unit of Structural Dynamics of Macromolecules, Institut Pasteur & CNRS UMR, Paris, France.
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Źródło :
PloS one [PLoS One] 2021 Apr 29; Vol. 16 (4), pp. e0250610. Date of Electronic Publication: 2021 Apr 29 (Print Publication: 2021).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cloning, Molecular*/methods
Coronavirus RNA-Dependent RNA Polymerase/*genetics
Escherichia coli/*genetics
SARS-CoV-2/*genetics
Viral Nonstructural Proteins/*genetics
COVID-19/virology ; Coronavirus RNA-Dependent RNA Polymerase/isolation & purification ; Coronavirus RNA-Dependent RNA Polymerase/metabolism ; Humans ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; SARS-CoV-2/metabolism ; Viral Nonstructural Proteins/isolation & purification ; Viral Nonstructural Proteins/metabolism
Czasopismo naukowe
Tytuł :
Biosynthesis of Long-Chain Polyunsaturated Fatty Acids in Marine Gammarids: Molecular Cloning and Functional Characterisation of Three Fatty Acyl Elongases.
Autorzy :
Ribes-Navarro A; Instituto de Acuicultura de Torre de la Sal (IATS-CSIC), 12595 Ribera de Cabanes, Castellón, Spain.
Navarro JC; Instituto de Acuicultura de Torre de la Sal (IATS-CSIC), 12595 Ribera de Cabanes, Castellón, Spain.
Hontoria F; Instituto de Acuicultura de Torre de la Sal (IATS-CSIC), 12595 Ribera de Cabanes, Castellón, Spain.
Kabeya N; Department of Marine Biosciences, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
Standal IB; Department of Fisheries and New Biomarine Industry, SINTEF Ocean, 7010 Trondheim, Norway.
Evjemo JO; Department of Fisheries and New Biomarine Industry, SINTEF Ocean, 7010 Trondheim, Norway.
Monroig Ó; Instituto de Acuicultura de Torre de la Sal (IATS-CSIC), 12595 Ribera de Cabanes, Castellón, Spain.
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Źródło :
Marine drugs [Mar Drugs] 2021 Apr 16; Vol. 19 (4). Date of Electronic Publication: 2021 Apr 16.
Typ publikacji :
Journal Article
MeSH Terms :
Cloning, Molecular*
Amphipoda/*enzymology
Fatty Acid Elongases/*metabolism
Fatty Acids, Unsaturated/*biosynthesis
Amphipoda/genetics ; Animals ; Evolution, Molecular ; Fatty Acid Elongases/genetics ; Gene Expression Regulation, Enzymologic ; Phylogeny ; Substrate Specificity
Czasopismo naukowe
Tytuł :
Forkhead transcription factor O1 (FoxO1) in torafugu pufferfish Takifugu rubripes: Molecular cloning, in vitro DNA binding, and target gene screening in fish metagenome.
Autorzy :
Reyes AC; School of Arts & Sciences, University of Houston-Victoria, Victoria, TX 77901, USA.
Egwu E; School of Arts & Sciences, University of Houston-Victoria, Victoria, TX 77901, USA.
Yu E; School of Arts & Sciences, University of Houston-Victoria, Victoria, TX 77901, USA; Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute of CAFS, Xingyu Road No. 1, Guangzhou 510380, China.
Sanchez AN; School of Arts & Sciences, University of Houston-Victoria, Victoria, TX 77901, USA.
De La O L; School of Arts & Sciences, University of Houston-Victoria, Victoria, TX 77901, USA.
Elijah OE; School of Arts & Sciences, University of Houston-Victoria, Victoria, TX 77901, USA.
Muschalek TJ; School of Arts & Sciences, University of Houston-Victoria, Victoria, TX 77901, USA.
Zhang W; School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang 453003, China.
Ji H; College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Ehsan H; School of Arts & Sciences, University of Houston-Victoria, Victoria, TX 77901, USA.
Kaneko G; School of Arts & Sciences, University of Houston-Victoria, Victoria, TX 77901, USA. Electronic address: .
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Źródło :
Gene [Gene] 2021 Feb 05; Vol. 768, pp. 145335. Date of Electronic Publication: 2020 Dec 02.
Typ publikacji :
Journal Article
MeSH Terms :
Cloning, Molecular*/drug effects
Fish Proteins/*genetics
Fish Proteins/*metabolism
Forkhead Box Protein O1/*genetics
Forkhead Box Protein O1/*metabolism
Takifugu/*metabolism
Animals ; Binding Sites ; Computer Simulation ; DNA/metabolism ; Gene Expression Regulation ; Gene Regulatory Networks ; Introns ; Phylogeny ; Protein Binding ; Takifugu/classification ; Takifugu/genetics
Czasopismo naukowe
Tytuł :
Molecular cloning, the characterization of metallothionein and catalase, and the evaluation of testicular toxicity of Cd in the Chinese fire-bellied newt (Cynops orientalis).
Autorzy :
Ruan SL; College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, PR China.
Xie L; College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, PR China; National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Wenzhou University, Wenzhou 325035, PR China.
Ou JW; College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, PR China; National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Wenzhou University, Wenzhou 325035, PR China.
Sun XS; College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, PR China.
Zhang YP; College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, PR China; National and Local Joint Engineering Research Center of Ecological Treatment Technology for Urban Water Pollution, Wenzhou University, Wenzhou 325035, PR China. Electronic address: .
Hu JR; College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, PR China. Electronic address: .
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Źródło :
Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2021 Jan 15; Vol. 208, pp. 111731. Date of Electronic Publication: 2020 Dec 04.
Typ publikacji :
Journal Article
MeSH Terms :
Cloning, Molecular*
Cadmium/*toxicity
Catalase/*metabolism
Hazardous Substances/*toxicity
Metallothionein/*metabolism
Salamandridae/*physiology
Testis/*drug effects
Animals ; Base Sequence ; Humans ; Liver/metabolism ; Male ; RNA, Messenger/metabolism ; Salamandridae/genetics ; Salamandridae/metabolism ; Sertoli Cells/metabolism ; Spermatozoa/metabolism ; Testis/metabolism
Czasopismo naukowe
Tytuł :
Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering.
Autorzy :
Jiang S; CAS Key Laboratory of Quantitative Engineering Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Luo Z; CAS Key Laboratory of Quantitative Engineering Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China.
Dai J; CAS Key Laboratory of Quantitative Engineering Biology, Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic Genomics, Shenzhen Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, China. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2196, pp. 167-180.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cloning, Molecular*/methods
Metabolic Engineering*/methods
Genetic Vectors/*genetics
Yeasts/*genetics
Yeasts/*metabolism
Gene Expression ; Gene Order ; Open Reading Frames ; Polymerase Chain Reaction ; Saccharomycetales/genetics ; Saccharomycetales/metabolism ; Synthetic Biology/methods ; Transformation, Genetic
Czasopismo naukowe
Tytuł :
Screening and Production of Recombinant Human Proteins: Ligation-Independent Cloning.
Autorzy :
Strain-Damerell C; Diamond Light Source Ltd., Didcot, Oxfordshire, UK.
Mahajan P; Astex Pharmaceuticals, Cambridge, UK.
Fernandez-Cid A; Structural Genomics Consortium, University of Oxford, Oxford, UK.
Gileadi O; Structural Genomics Consortium, University of Oxford, Oxford, UK.
Burgess-Brown NA; Structural Genomics Consortium, University of Oxford, Oxford, UK. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2199, pp. 23-43.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cloning, Molecular*
Escherichia coli*/genetics
Escherichia coli*/metabolism
Gene Expression*
Recombinant Proteins*/biosynthesis
Recombinant Proteins*/genetics
Baculoviridae/*genetics
Genetic Vectors/*genetics
Humans
Czasopismo naukowe
Tytuł :
Molecular Expression of Bioactive Recombinant Methionine Sulfoxide Reductase A (MsrA).
Autorzy :
Indhu MS; Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar - 243122, Bareilly, U.P., India.
Nanjundappa S; Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar - 243122, Bareilly, U.P., India.
Muttu R; Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar - 243122, Bareilly, U.P., India.
Vikramaditya U; Division of Biological Standardization, Indian Veterinary Research Institute, Izatnagar - 243122, Bareilly, U.P., India.
Mahawar M; Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar - 243122, Bareilly, U.P., India.
Sarkar M; Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar - 243122, Bareilly, U.P., India.
Guttula TS; Division of Physiology and Climatology, Indian Veterinary Research Institute, Izatnagar - 243122, Bareilly, U.P., India.
Bhure S; Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar - 243122, Bareilly, U.P., India.
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Źródło :
Protein and peptide letters [Protein Pept Lett] 2021; Vol. 28 (1), pp. 11-17.
Typ publikacji :
Journal Article
MeSH Terms :
Cloning, Molecular*
Gene Expression*
Methionine Sulfoxide Reductases*/biosynthesis
Methionine Sulfoxide Reductases*/chemistry
Methionine Sulfoxide Reductases*/genetics
Animals ; Cattle ; Male ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics
Czasopismo naukowe
Tytuł :
High-Throughput Expression Screening in Mammalian Suspension Cells.
Autorzy :
Chapple SD; IONTAS Ltd, Unit 2, Iconix Park, London Road, Pampisford, Cambridge, UK.
Dyson MR; Department of Antibody Engineering, Ichnos Sciences S.A., Biopôle Lausanne-Epalinges, Epalinges, Switzerland. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2199, pp. 117-125.
Typ publikacji :
Journal Article
MeSH Terms :
Cloning, Molecular*
Gene Expression*
High-Throughput Screening Assays*
Recombinant Proteins*/biosynthesis
Recombinant Proteins*/genetics
Transfection*
Genetic Vectors/*genetics
HEK293 Cells ; Humans
Czasopismo naukowe
Tytuł :
InteBac: An integrated bacterial and baculovirus expression vector suite.
Autorzy :
Altmannova V; Friedrich Miescher Laboratory of the Max-Planck-Society, Max-Planck-Ring 9, Tübingen, Germany.
Blaha A; Friedrich Miescher Laboratory of the Max-Planck-Society, Max-Planck-Ring 9, Tübingen, Germany.
Astrinidis S; Friedrich Miescher Laboratory of the Max-Planck-Society, Max-Planck-Ring 9, Tübingen, Germany.
Reichle H; Friedrich Miescher Laboratory of the Max-Planck-Society, Max-Planck-Ring 9, Tübingen, Germany.
Weir JR; Friedrich Miescher Laboratory of the Max-Planck-Society, Max-Planck-Ring 9, Tübingen, Germany.
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Źródło :
Protein science : a publication of the Protein Society [Protein Sci] 2021 Jan; Vol. 30 (1), pp. 108-114. Date of Electronic Publication: 2020 Oct 13.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cloning, Molecular*
Gene Expression*
Baculoviridae/*genetics
Escherichia coli/*genetics
Genetic Vectors/*genetics
Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics
Czasopismo naukowe
Tytuł :
Gene cloning, heterologous expression, and partial characterization of a novel cold-adapted subfamily I.3 lipase from Pseudomonas fluorescence KE38.
Autorzy :
Karakaş F; Department of Molecular Biology and Genetics, Faculty of Science, Izmir Institute of Technology, Izmir, Turkey.
Arslanoğlu A; Department of Molecular Biology and Genetics, Faculty of Science, Izmir Institute of Technology, Izmir, Turkey. .
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Źródło :
Scientific reports [Sci Rep] 2020 Dec 16; Vol. 10 (1), pp. 22063. Date of Electronic Publication: 2020 Dec 16.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Acclimatization*
Bacterial Proteins*/biosynthesis
Bacterial Proteins*/chemistry
Bacterial Proteins*/genetics
Bacterial Proteins*/isolation & purification
Cloning, Molecular*
Cold Temperature*
Gene Expression*
Lipase*/biosynthesis
Lipase*/chemistry
Lipase*/genetics
Lipase*/isolation & purification
Pseudomonas fluorescens*/enzymology
Pseudomonas fluorescens*/genetics
Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification
Czasopismo naukowe
Tytuł :
Gene cloning, expression enhancement in Escherichia coli and biochemical characterization of a highly thermostable amylomaltase from Pyrobaculum calidifontis.
Autorzy :
Mehboob S; School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan; Kinnaird College for Women, 93 Jail Road, Lahore 54000, Pakistan.
Ahmad N; School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.
Munir S; Kinnaird College for Women, 93 Jail Road, Lahore 54000, Pakistan.
Ali R; School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.
Younas H; Kinnaird College for Women, 93 Jail Road, Lahore 54000, Pakistan.
Rashid N; School of Biological Sciences, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan. Electronic address: .
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Źródło :
International journal of biological macromolecules [Int J Biol Macromol] 2020 Dec 15; Vol. 165 (Pt A), pp. 645-653. Date of Electronic Publication: 2020 Sep 17.
Typ publikacji :
Journal Article
MeSH Terms :
Archaeal Proteins*/biosynthesis
Archaeal Proteins*/chemistry
Archaeal Proteins*/genetics
Archaeal Proteins*/isolation & purification
Cloning, Molecular*
Gene Expression*
Glycogen Debranching Enzyme System*/biosynthesis
Glycogen Debranching Enzyme System*/chemistry
Glycogen Debranching Enzyme System*/genetics
Glycogen Debranching Enzyme System*/isolation & purification
Hot Temperature*
Pyrobaculum*/enzymology
Pyrobaculum*/genetics
Enzyme Stability ; Escherichia coli/enzymology ; Escherichia coli/genetics ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification
Czasopismo naukowe
Tytuł :
Baculovirus-free insect cell expression system for high yield antibody and antigen production.
Autorzy :
Korn J; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Schäckermann D; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Kirmann T; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.; Medical Faculty, Carl Ludwig Institute for Physiology, Universität Leipzig, Liebigstraße 27, 04103, Leipzig, Germany.
Bertoglio F; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Steinke S; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Heisig J; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.; Department Vaccinology and Applied Microbiology, Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124, Braunschweig, Germany.
Ruschig M; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Rojas G; Center of Molecular Immunology, PO Box 16040, 11300, Havana, Cuba.
Langreder N; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Wenzel EV; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Roth KDR; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Becker M; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Meier D; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
van den Heuvel J; Department Structure and Function of Proteins, Helmholtz-Centre for Infection Research, Inhoffenstraße 7, 38124, Braunschweig, Germany.
Hust M; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Dübel S; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany.
Schubert M; Department of Biotechnology, Technische Universität Braunschweig, Spielmannstraße 7, 38106, Braunschweig, Germany. .
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Źródło :
Scientific reports [Sci Rep] 2020 Dec 07; Vol. 10 (1), pp. 21393. Date of Electronic Publication: 2020 Dec 07.
Typ publikacji :
Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cloning, Molecular*
Antibodies, Monoclonal/*biosynthesis
Antibodies, Neutralizing/*biosynthesis
Antigens, Viral/*biosynthesis
Recombinant Proteins/*biosynthesis
SARS-CoV-2/*immunology
Animals ; HEK293 Cells ; Humans ; Protein Stability ; Spodoptera
Czasopismo naukowe
Tytuł :
The pMy vector series: A versatile cloning platform for the recombinant production of mycobacterial proteins in Mycobacterium smegmatis.
Autorzy :
Beckham KSH; Hamburg Unit, European Molecular Biology Laboratory, Hamburg, Germany.
Staack S; Hamburg Unit, European Molecular Biology Laboratory, Hamburg, Germany.
Wilmanns M; Hamburg Unit, European Molecular Biology Laboratory, Hamburg, Germany.; University Hamburg Clinical Centre Hamburg-Eppendorf, Hamburg, Germany.
Parret AHA; Hamburg Unit, European Molecular Biology Laboratory, Hamburg, Germany.
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Źródło :
Protein science : a publication of the Protein Society [Protein Sci] 2020 Dec; Vol. 29 (12), pp. 2528-2537. Date of Electronic Publication: 2020 Oct 13.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cloning, Molecular*
Genetic Vectors*
Mycobacterium smegmatis*/genetics
Mycobacterium smegmatis*/metabolism
Plasmids*/genetics
Plasmids*/metabolism
Escherichia coli/genetics ; Escherichia coli/metabolism ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/metabolism ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/genetics
Czasopismo naukowe
Tytuł :
Bacillus sonorensis L. Asparaginase: Cloning, Expression in E. coli and Characterization.
Autorzy :
Aly N; Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
El-Ahwany A; Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Ataya FS; Biochemistry Department, College of Science, King Saud University, Bld. 5, Lab AA10, P.O. Box: 2454, Riyadh, Kingdom of Saudi Arabia.; National Research Centre, 33 El-Bohouth St. (former El-Tahrir St.), Dokki, P.O. 12622, Giza, Egypt.
Saeed H; Department of Biotechnology, Institute of Graduate Studies and Research, Alexandria University, Alexandria, Egypt. .
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Źródło :
The protein journal [Protein J] 2020 Dec; Vol. 39 (6), pp. 717-729. Date of Electronic Publication: 2020 Oct 26.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Asparaginase*/biosynthesis
Asparaginase*/chemistry
Asparaginase*/genetics
Bacillus*/enzymology
Bacillus*/genetics
Bacterial Proteins*/biosynthesis
Bacterial Proteins*/chemistry
Bacterial Proteins*/genetics
Cloning, Molecular*
Gene Expression*
Enzyme Stability ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics
SCR Organism :
Bacillus sonorensis
Czasopismo naukowe
Tytuł :
Functional expression and purification of the untagged C-terminal domain of MMP-2 from Escherichia coli inclusion bodies.
Autorzy :
Zhou Y; School of Chemistry and Chemical Engineering, South China University of Technology, 510640, Guangzhou, China.
He C; School of Chemistry and Chemical Engineering, South China University of Technology, 510640, Guangzhou, China. Electronic address: .
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Źródło :
Protein expression and purification [Protein Expr Purif] 2020 Dec; Vol. 176, pp. 105726. Date of Electronic Publication: 2020 Aug 11.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cloning, Molecular*
Escherichia coli*/chemistry
Escherichia coli*/genetics
Escherichia coli*/metabolism
Gene Expression*
Matrix Metalloproteinase 2*/biosynthesis
Matrix Metalloproteinase 2*/chemistry
Matrix Metalloproteinase 2*/genetics
Matrix Metalloproteinase 2*/isolation & purification
Inclusion Bodies/*chemistry
Humans ; Inclusion Bodies/genetics ; Inclusion Bodies/metabolism ; Protein Domains
Czasopismo naukowe
Tytuł :
Cloning and characterization of a novel chondroitinase ABC categorized into a new subfamily of polysaccharide lyase family 8.
Autorzy :
Zhang Z; Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, 5 Yushan Road, Qingdao 266003, PR China; School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, PR China.
Su H; Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, 5 Yushan Road, Qingdao 266003, PR China; School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, PR China.
Wang X; Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, 5 Yushan Road, Qingdao 266003, PR China; School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, PR China.
Tang L; Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, 5 Yushan Road, Qingdao 266003, PR China; School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, PR China.
Hu J; Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, 5 Yushan Road, Qingdao 266003, PR China; School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, PR China.
Yu W; Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, 5 Yushan Road, Qingdao 266003, PR China; School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, PR China.
Han F; Key Laboratory of Marine Drugs, Chinese Ministry of Education, 5 Yushan Road, Qingdao 266003, PR China; Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, 5 Yushan Road, Qingdao 266003, PR China; School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 266237, PR China.. Electronic address: .
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Źródło :
International journal of biological macromolecules [Int J Biol Macromol] 2020 Dec 01; Vol. 164, pp. 3762-3770. Date of Electronic Publication: 2020 Aug 29.
Typ publikacji :
Journal Article
MeSH Terms :
Cloning, Molecular*
Chondroitin ABC Lyase/*genetics
Polysaccharide-Lyases/*genetics
Vibrio/*genetics
Chondroitin Lyases/genetics ; Chondroitin Sulfates/genetics ; Glycosaminoglycans/genetics ; Oligosaccharides/genetics ; Phylogeny ; Substrate Specificity ; Vibrio/enzymology
Czasopismo naukowe
Tytuł :
Cloning, expression, and purification of porcine adrenocorticotropic hormone in Escherichia coli.
Autorzy :
Wu Y; Department of Microbiological and Biochemical Pharmacy, School of Pharmacy, Fudan University, Shanghai, 201203, China; Shanghai Duomirui Biotechnology Ltd, China State Institute of Pharmaceutical Industry, Shanghai, 201203, China.
Hua H; Shanghai Duomirui Biotechnology Ltd, China State Institute of Pharmaceutical Industry, Shanghai, 201203, China.
Huang Z; Shanghai Duomirui Biotechnology Ltd, China State Institute of Pharmaceutical Industry, Shanghai, 201203, China.
Feng M; Department of Microbiological and Biochemical Pharmacy, School of Pharmacy, Fudan University, Shanghai, 201203, China. Electronic address: .
Feng J; Shanghai Duomirui Biotechnology Ltd, China State Institute of Pharmaceutical Industry, Shanghai, 201203, China. Electronic address: .
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Źródło :
Protein expression and purification [Protein Expr Purif] 2020 Dec; Vol. 176, pp. 105731. Date of Electronic Publication: 2020 Aug 29.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Adrenocorticotropic Hormone*/biosynthesis
Adrenocorticotropic Hormone*/chemistry
Adrenocorticotropic Hormone*/genetics
Adrenocorticotropic Hormone*/isolation & purification
Cloning, Molecular*
Gene Expression*
Recombinant Fusion Proteins*/biosynthesis
Recombinant Fusion Proteins*/chemistry
Recombinant Fusion Proteins*/genetics
Recombinant Fusion Proteins*/isolation & purification
Swine/*genetics
Animals ; Escherichia coli/genetics ; Escherichia coli/metabolism
Czasopismo naukowe
Tytuł :
Cloning, expression and purification of recombinant dermatopontin in Escherichia coli.
Autorzy :
Seetaraman Amritha TM; Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India.
Mahajan S; SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
Subramaniam K; Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India.
Chandramohan Y; Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India.
Dhanasekaran A; Centre for Biotechnology, Anna University, Chennai, Tamil Nadu, India.
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Źródło :
PloS one [PLoS One] 2020 Nov 30; Vol. 15 (11), pp. e0242798. Date of Electronic Publication: 2020 Nov 30 (Print Publication: 2020).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Cloning, Molecular*
Chondroitin Sulfate Proteoglycans/*genetics
Extracellular Matrix Proteins/*genetics
Recombinant Proteins/*genetics
Cell Movement/genetics ; Chondroitin Sulfate Proteoglycans/biosynthesis ; Endothelial Cells/metabolism ; Escherichia coli/genetics ; Extracellular Matrix Proteins/biosynthesis ; Humans ; Inclusion Bodies/genetics ; Inclusion Bodies/metabolism ; Protein Folding ; Recombinant Proteins/biosynthesis ; Wound Healing/genetics
Czasopismo naukowe
Tytuł :
Cloning, expression, identification and characterization of borneol dehydrogenase isozymes in Pseudomonas sp. TCU-HL1.
Autorzy :
Khine AA; Department of Biochemistry, Tzu Chi University, Hualien, 97004, Taiwan; Institute of Medical Sciences, School of Medicine, Tzu Chi University, Hualien, 97004, Taiwan; Department of Biochemistry, University of Medicine 1, Yangon, Myanmar.
Lu PC; Department of Biochemistry, Tzu Chi University, Hualien, 97004, Taiwan.
Ko TP; Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan.
Huang KF; Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan.
Chen HP; Department of Biochemistry, Tzu Chi University, Hualien, 97004, Taiwan; Institute of Medical Sciences, School of Medicine, Tzu Chi University, Hualien, 97004, Taiwan; Integration Center of Traditional Chinese and Modern Medicine, Hualien Tzu Chi Hospital, Hualien, 97002, Taiwan. Electronic address: .
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Źródło :
Protein expression and purification [Protein Expr Purif] 2020 Nov; Vol. 175, pp. 105715. Date of Electronic Publication: 2020 Jul 29.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Alcohol Oxidoreductases*/biosynthesis
Alcohol Oxidoreductases*/chemistry
Alcohol Oxidoreductases*/genetics
Alcohol Oxidoreductases*/isolation & purification
Bacterial Proteins*/biosynthesis
Bacterial Proteins*/chemistry
Bacterial Proteins*/genetics
Bacterial Proteins*/isolation & purification
Cloning, Molecular*
Gene Expression*
Pseudomonas*/enzymology
Pseudomonas*/genetics
Czasopismo naukowe

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