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Wyszukujesz frazę ""Cloning, Molecular"" wg kryterium: Temat


Tytuł:
Cloning, expression, solubilization, and purification of a functionally active recombinant cAMP-dependent protein kinase catalytic subunit-like protein PKAC1 from Trypanosoma equiperdum.
Autorzy:
Guevara A; Departamento de Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela; Postgrado en Ciencias Biológicas, Universidad Simón Bolívar, Caracas, Venezuela.
Lugo C; Departamento de Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela; Postgrado en Ciencias Biológicas, Universidad Simón Bolívar, Caracas, Venezuela.
Montilla AJ; Departamento de Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela; Postgrado en Ciencias Biológicas, Universidad Simón Bolívar, Caracas, Venezuela.
Calabokis M; Departamento de Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela.
Ferreira J; Postgrado en Ciencias Biológicas, Universidad Simón Bolívar, Caracas, Venezuela; Dirección de Salud, Fundación Instituto de Estudios Avanzados IDEA, Caracas, 1015-A, Venezuela.
Martínez JC; Dirección de Salud, Fundación Instituto de Estudios Avanzados IDEA, Caracas, 1015-A, Venezuela.
Bubis J; Departamento de Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela. Electronic address: .
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Źródło:
Protein expression and purification [Protein Expr Purif] 2022 Apr; Vol. 192, pp. 106041. Date of Electronic Publication: 2021 Dec 23.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cloning, Molecular*
Cyclic AMP/*metabolism
Cyclic AMP-Dependent Protein Kinases/*genetics
Cyclic AMP-Dependent Protein Kinases/*metabolism
Protozoan Proteins/*genetics
Protozoan Proteins/*metabolism
Trypanosoma/*enzymology
Cyclic AMP/genetics ; Cyclic AMP-Dependent Protein Kinases/chemistry ; Cyclic AMP-Dependent Protein Kinases/isolation & purification ; Phosphorylation ; Protein Subunits/chemistry ; Protein Subunits/genetics ; Protein Subunits/isolation & purification ; Protein Subunits/metabolism ; Protozoan Proteins/chemistry ; Protozoan Proteins/isolation & purification ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/isolation & purification ; Recombinant Proteins/metabolism ; Solubility ; Trypanosoma/chemistry ; Trypanosoma/genetics
Czasopismo naukowe
Tytuł:
Next-generation sequencing confirms T-cell clonality in a subset of pediatric pityriasis lichenoides.
Autorzy:
Raghavan SS; Department of Pathology, University of Virginia Medical Center, Charlottesville, Virginia, USA.
Wang JY; Department of Dermatology, Stanford University Medical Center, Stanford, California, USA.
Gru AA; Department of Pathology, University of Virginia Medical Center, Charlottesville, Virginia, USA.
Marqueling AL; Department of Dermatology, Stanford University Medical Center, Stanford, California, USA.
Teng JMC; Department of Dermatology, Stanford University Medical Center, Stanford, California, USA.
Brown RA; Department of Dermatology, Stanford University Medical Center, Stanford, California, USA.; Department of Pathology, Stanford University Medical Center, Stanford, California, USA.
Novoa RA; Department of Dermatology, Stanford University Medical Center, Stanford, California, USA.; Department of Pathology, Stanford University Medical Center, Stanford, California, USA.
Kim Y; Department of Dermatology, Stanford University Medical Center, Stanford, California, USA.
Zehnder J; Department of Pathology, Stanford University Medical Center, Stanford, California, USA.
Zhang BM; Department of Pathology, Stanford University Medical Center, Stanford, California, USA.
Rieger KE; Department of Dermatology, Stanford University Medical Center, Stanford, California, USA.; Department of Pathology, Stanford University Medical Center, Stanford, California, USA.
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Źródło:
Journal of cutaneous pathology [J Cutan Pathol] 2022 Mar; Vol. 49 (3), pp. 252-260. Date of Electronic Publication: 2021 Oct 21.
Typ publikacji:
Journal Article
MeSH Terms:
Cloning, Molecular*
Genes, T-Cell Receptor beta/*genetics
Genes, T-Cell Receptor gamma/*genetics
Pityriasis Lichenoides/*genetics
Adolescent ; Child ; Child, Preschool ; Female ; High-Throughput Nucleotide Sequencing ; Humans ; Male
Czasopismo naukowe
Tytuł:
Expression and Surface Display of an Acidic Cold-Active Chitosanase in Pichia pastoris Using Multi-Copy Expression and High-Density Cultivation.
Autorzy:
Peng Y; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Wang Y; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Liu X; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Zhou R; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Liao X; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Min Y; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Ma L; State Key Laboratory of Biocatalysis and Enzyme, Engineering Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, Hubei Key Laboratory of Industrial Biotechnology, Biology Faculty of Hubei University, Hubei University, Wuhan 430062, China.
Wang Y; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
Rao B; National Biopesticide Engineering Technology Research Center, Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Sciences, Biopesticide Branch of Hubei Innovation Centre of Agricultural Science and Technology, Wuhan 430068, China.
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Źródło:
Molecules (Basel, Switzerland) [Molecules] 2022 Jan 26; Vol. 27 (3). Date of Electronic Publication: 2022 Jan 26.
Typ publikacji:
Journal Article
MeSH Terms:
Cloning, Molecular*
Bacillus/*genetics
Bacterial Proteins/*genetics
Glycoside Hydrolases/*genetics
Pichia/*genetics
Bacillus/metabolism ; Bacterial Proteins/metabolism ; Cold Temperature ; Enzyme Stability ; Fermentation ; Gene Expression ; Glycoside Hydrolases/metabolism ; Pichia/metabolism ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism
SCR Organism:
Bacillus glycinifermentans
Czasopismo naukowe
Tytuł:
Cloning and Characterization of Three Novel Enzymes Responsible for the Detoxification of Zearalenone.
Autorzy:
Zhang Y; School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, China.
Liu X; Beijing Key Laboratory of Nutrition & Health and Food Safety, Nutrition and Health Research Institute, COFCO, Beijing 102209, China.
Zhang Y; Beijing Key Laboratory of Nutrition & Health and Food Safety, Nutrition and Health Research Institute, COFCO, Beijing 102209, China.
Zhang X; Beijing Key Laboratory of Nutrition & Health and Food Safety, Nutrition and Health Research Institute, COFCO, Beijing 102209, China.
Huang H; School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, China.
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Źródło:
Toxins [Toxins (Basel)] 2022 Jan 21; Vol. 14 (2). Date of Electronic Publication: 2022 Jan 21.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cloning, Molecular*
Ascomycota/*enzymology
Fungal Proteins/*metabolism
Zearalenone/*metabolism
Zearalenone/*toxicity
Amino Acid Sequence ; Fungal Proteins/genetics ; Gene Expression Regulation, Enzymologic/physiology ; Gene Expression Regulation, Fungal/physiology
Czasopismo naukowe
Tytuł:
A Recombinant System and Reporter Viruses for Papiine Alphaherpesvirus 2.
Autorzy:
Rahman Siregar A; German Primate Center, Infection Biology Unit, Leibniz Institute for Primate Research, 37077 Gottingen, Germany.; Faculty of Biology and Psychology, University Göttingen, 30073 Gottingen, Germany.; Faculty of Biology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
Gärtner S; German Primate Center, Infection Biology Unit, Leibniz Institute for Primate Research, 37077 Gottingen, Germany.
Götting J; Institute of Virology, Hannover Medical School, 30625 Hannover, Germany.
Stegen P; German Primate Center, Infection Biology Unit, Leibniz Institute for Primate Research, 37077 Gottingen, Germany.
Kaul A; German Primate Center, Infection Biology Unit, Leibniz Institute for Primate Research, 37077 Gottingen, Germany.
Schulz TF; Institute of Virology, Hannover Medical School, 30625 Hannover, Germany.
Pöhlmann S; German Primate Center, Infection Biology Unit, Leibniz Institute for Primate Research, 37077 Gottingen, Germany.; Faculty of Biology and Psychology, University Göttingen, 30073 Gottingen, Germany.
Winkler M; German Primate Center, Infection Biology Unit, Leibniz Institute for Primate Research, 37077 Gottingen, Germany.
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Źródło:
Viruses [Viruses] 2022 Jan 05; Vol. 14 (1). Date of Electronic Publication: 2022 Jan 05.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cloning, Molecular*
Genome, Viral*
Recombination, Genetic*
Simplexvirus/*genetics
Animals ; Antibodies, Viral/immunology ; Antiviral Agents/pharmacology ; Cell Line ; Genes, Reporter ; Humans ; Papio/immunology ; Simplexvirus/immunology ; Simplexvirus/pathogenicity ; Simplexvirus/physiology
Czasopismo naukowe
Tytuł:
Cloning, Expression and Inhibitory Effects on Lewis Lung Carcinoma Cells of rAj-Tspin from Sea Cucumber ( Apostichopus japonicus ).
Autorzy:
Qiao R; Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China.; School of Life Sciences, Liaoning Normal University, Dalian 116081, China.
Xiao R; School of Life Sciences, Liaoning Normal University, Dalian 116081, China.
Chen Z; Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China.
Jiang J; Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China.
Yuan C; School of Life Sciences, Liaoning Normal University, Dalian 116081, China.
Ning S; School of Life Sciences, Liaoning Normal University, Dalian 116081, China.
Wang J; School of Life Sciences, Liaoning Normal University, Dalian 116081, China.
Zhou Z; Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China.
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Źródło:
Molecules (Basel, Switzerland) [Molecules] 2021 Dec 30; Vol. 27 (1). Date of Electronic Publication: 2021 Dec 30.
Typ publikacji:
Journal Article
MeSH Terms:
Cloning, Molecular*
Gene Expression*
Recombinant Proteins*
Antineoplastic Agents/*pharmacology
Metalloendopeptidases/*genetics
Metalloendopeptidases/*pharmacology
Sea Cucumbers/*genetics
Amino Acid Sequence ; Animals ; Apoptosis ; Carcinoma, Lewis Lung ; Cell Line, Tumor ; Cell Movement/drug effects ; Dose-Response Relationship, Drug ; Extracellular Matrix Proteins/metabolism ; Metalloendopeptidases/chemistry ; Metalloendopeptidases/metabolism ; Phylogeny ; Protein Interaction Domains and Motifs ; Sea Cucumbers/classification ; Sea Cucumbers/metabolism ; Solubility
Czasopismo naukowe
Tytuł:
Cloning and over Expression Studies of Ovine Somatotropin cDNA of Kajli (sheep breed) in a Prokaryotic System.
Autorzy:
Tufail MS; Microbiology Lab, Department of Zoology, Government College University.
Liaqat I; Microbiology Lab, Department of Zoology, Government College University.
Sadiqa A; Department of Chemistry, University of Engineering and Technology.
Mushtaq M; Department of Chemistry, Government College University.
Andleeb S; Department of Zoology, University of Azad Jammu and Kashmir.
Liaqat I; Microbiology Lab, Department of Zoology, Government College University.
Munir N; Department of Botany, Lahore College for Women University.
Bibi A; Department of Zoology, The Women University.
Zain S; Molecular biology Lab, Department of Zoology, Government College University.
Fatima N; Molecular biology Lab, Department of Zoology, Government College University.
Saleem G; Department of Pathology, University of Veterinary and Animal Sciences.
Rasheed F; Department of Zoology, Lahore College for Women University.
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Źródło:
Journal of oleo science [J Oleo Sci] 2021 Dec 03; Vol. 70 (12), pp. 1791-1796. Date of Electronic Publication: 2021 Nov 09.
Typ publikacji:
Journal Article
MeSH Terms:
Cloning, Molecular*
Gene Expression*
DNA, Complementary/*genetics
DNA, Complementary/*metabolism
Growth Hormone/*genetics
Prokaryotic Cells/*metabolism
Sheep/*genetics
Animals ; Growth Hormone/metabolism ; Polymerase Chain Reaction ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism
Czasopismo naukowe
Tytuł:
Cloning and reproductive regulation of a trypsin precursor gene in Adelphocorissuturalis.
Autorzy:
Huang X; National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Zhu B; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China; Guiyang Center for Disease Control and Prevention, Guiyang 550003, Guizhou, China.
Zhang W; National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, Hubei, China; College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: .
Chen L; Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei, China. Electronic address: .
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Źródło:
International journal of biological macromolecules [Int J Biol Macromol] 2021 Dec 01; Vol. 192, pp. 38-44. Date of Electronic Publication: 2021 Sep 28.
Typ publikacji:
Journal Article
MeSH Terms:
Cloning, Molecular*
Gene Expression*
Heteroptera/*genetics
Protein Precursors/*genetics
Reproduction/*genetics
Trypsin/*genetics
Amino Acid Sequence ; Animals ; Base Sequence ; Female ; Fertility/genetics ; Gene Expression Regulation ; Heteroptera/classification ; Heteroptera/enzymology ; Phylogeny ; Protein Precursors/metabolism ; Sequence Analysis, DNA ; Trypsin/metabolism
Czasopismo naukowe
Tytuł:
Molecular cloning and functional characterization of UGTs from Glycyrrhiza uralensis flavonoid pathway.
Autorzy:
Jiang D; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Li P; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Yin Y; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Ren G; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China.
Liu C; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. Electronic address: max_.
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Źródło:
International journal of biological macromolecules [Int J Biol Macromol] 2021 Dec 01; Vol. 192, pp. 1108-1116. Date of Electronic Publication: 2021 Sep 25.
Typ publikacji:
Journal Article
MeSH Terms:
Cloning, Molecular*
Gene Expression*
Flavonoids/*metabolism
Glycosyltransferases/*genetics
Glycosyltransferases/*metabolism
Glycyrrhiza uralensis/*enzymology
Glycyrrhiza uralensis/*genetics
Amino Acid Sequence ; Catalytic Domain ; Chromatography, High Pressure Liquid ; Enzyme Activation ; Gene Expression Profiling ; Glycosyltransferases/chemistry ; Glycyrrhiza uralensis/classification ; Metabolic Networks and Pathways ; Models, Molecular ; Molecular Conformation ; Molecular Structure ; Phylogeny ; Recombinant Proteins ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Czasopismo naukowe
Tytuł:
Cloning, functional and regulation analysis of a novel male reproduction-related protein gene from the oriental river prawn Macrobrachium nipponense.
Autorzy:
Li F; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang University of Science and Technology, Shouguang 262700, PR China. Electronic address: .
Li J; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang University of Science and Technology, Shouguang 262700, PR China.
Fu C; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang University of Science and Technology, Shouguang 262700, PR China.
Zhu H; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang University of Science and Technology, Shouguang 262700, PR China.
Yu L; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization, Weifang University of Science and Technology, Shouguang 262700, PR China.
Zhang Y; Shandong Freshwater Fisheries Research Institute, Jinan 250013, PR China. Electronic address: .
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Źródło:
Animal reproduction science [Anim Reprod Sci] 2021 Nov; Vol. 234, pp. 106867. Date of Electronic Publication: 2021 Sep 27.
Typ publikacji:
Journal Article
MeSH Terms:
Cloning, Molecular*
Gene Expression Regulation, Developmental/*physiology
Palaemonidae/*metabolism
Proteins/*metabolism
Animals ; Male ; Proteins/genetics ; RNA Interference ; Reproduction/physiology ; Testis/growth & development
Czasopismo naukowe
Tytuł:
In vivo cleavage of solubility tags as a tool to enhance the levels of soluble recombinant proteins in Escherichia coli.
Autorzy:
Silva FSR; Post-Graduate Program in Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.
Santos SPO; Post-Graduate Program in Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.
Meyer R; Post-Graduate Program in Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.
Silva ES; Post-Graduate Program in Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.
Pinheiro CS; Post-Graduate Program in Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.
Alcantara-Neves NM; Post-Graduate Program in Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.; Post-Graduate Program in Immunology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.
Pacheco LGC; Post-Graduate Program in Biotechnology, Institute of Health Sciences, Federal University of Bahia, Salvador, Bahia, Brazil.; Department of Biotechnology, Federal University of Bahia, Salvador, Bahia, Brazil.
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Źródło:
Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Nov; Vol. 118 (11), pp. 4159-4167. Date of Electronic Publication: 2021 Aug 14.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms:
Cloning, Molecular*
Escherichia coli*/genetics
Escherichia coli*/metabolism
Gene Expression*
Recombinant Fusion Proteins*/biosynthesis
Recombinant Fusion Proteins*/chemistry
Recombinant Fusion Proteins*/genetics
Recombinant Fusion Proteins*/isolation & purification
Endopeptidases/*chemistry
Czasopismo naukowe
Tytuł:
Cloning and characterization of a panel of mitochondrial targeting sequences for compartmentalization engineering in Saccharomyces cerevisiae.
Autorzy:
Dong C; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.; Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, China.
Shi Z; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.
Huang L; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.; Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, China.
Zhao H; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
Xu Z; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.
Lian J; Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, China.; Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, China.; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.
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Źródło:
Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Nov; Vol. 118 (11), pp. 4269-4277. Date of Electronic Publication: 2021 Jul 26.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
Cloning, Molecular*
Metabolic Engineering*
Metabolic Networks and Pathways*
Mitochondria*/genetics
Mitochondria*/metabolism
Saccharomyces cerevisiae*/genetics
Saccharomyces cerevisiae*/metabolism
Saccharomyces cerevisiae Proteins*/genetics
Saccharomyces cerevisiae Proteins*/metabolism
Escherichia coli/genetics ; Escherichia coli/metabolism
Czasopismo naukowe
Tytuł:
Cloning and identification of a new repressor of 3,17β-Hydroxysteroid dehydrogenase of Comamonas testosteroni.
Autorzy:
Xie W; School of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
Xia Q; School of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
Chen L; School of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
Xiong G; Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig-Holstein, 24103, Kiel, Germany.
Gao Y; Institute of Military Veterinary Medicine, Academy of Military Medical Sciences, Changchun, 130122, China.
Yu Y; School of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China.
He X; School of Life Science and Technology, Changchun University of Science and Technology, Changchun, 130022, China. .
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Źródło:
Molecular biology reports [Mol Biol Rep] 2021 Nov; Vol. 48 (11), pp. 7067-7075. Date of Electronic Publication: 2021 Oct 22.
Typ publikacji:
Journal Article
MeSH Terms:
Bacterial Proteins*/biosynthesis
Bacterial Proteins*/chemistry
Bacterial Proteins*/genetics
Cloning, Molecular*
Comamonas testosteroni*/chemistry
Comamonas testosteroni*/genetics
Comamonas testosteroni*/metabolism
Enzyme Inhibitors*/chemistry
Enzyme Inhibitors*/metabolism
Trans-Activators*/biosynthesis
Trans-Activators*/chemistry
Trans-Activators*/genetics
17-Hydroxysteroid Dehydrogenases/*antagonists & inhibitors
Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics
Czasopismo naukowe
Tytuł:
Label-free cell based impedance measurements of ZnO nanoparticles-human lung cell interaction: a comparison with MTT, NR, Trypan blue and cloning efficiency assays.
Autorzy:
Bozzuto G; National Centre for Drug Research and Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.
D'Avenio G; National Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, Rome, Italy.
Condello M; National Centre for Drug Research and Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.
Sennato S; CNR-ISC Sede Sapienza and Department of Physics, Sapienza University of Rome, Rome, Italy.
Battaglione E; Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome, Italy.
Familiari G; Department of Anatomical, Histological, Forensic Medicine and Orthopedics Sciences, Sapienza University of Rome, Rome, Italy.
Molinari A; National Centre for Drug Research and Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy. .
Grigioni M; National Centre for Innovative Technologies in Public Health, Istituto Superiore di Sanità, Rome, Italy.
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Źródło:
Journal of nanobiotechnology [J Nanobiotechnology] 2021 Oct 07; Vol. 19 (1), pp. 306. Date of Electronic Publication: 2021 Oct 07.
Typ publikacji:
Journal Article
MeSH Terms:
Cloning, Molecular*
Electric Impedance*
Metal Nanoparticles*/chemistry
Metal Nanoparticles*/toxicity
Toxicity Tests*
Zinc Oxide*/chemistry
Zinc Oxide*/toxicity
A549 Cells ; Apoptosis/drug effects ; Cell Survival/drug effects ; Humans ; Lung/cytology ; Microscopy, Confocal ; Microscopy, Electron ; Trypan Blue
Czasopismo naukowe
Tytuł:
Map-based cloning and promoter variation analysis of the lobed leaf gene BoLMI1a in ornamental kale (Brassica oleracea L. var. acephala).
Autorzy:
Zhang B; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Chen W; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Li X; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Ren W; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Chen L; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Han F; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Fang Z; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Yang L; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Zhuang M; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Lv H; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Wang Y; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China.
Zhang Y; Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, #12 Zhong Guan Cun Nandajie Street, Beijing, 100081, China. .
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Źródło:
BMC plant biology [BMC Plant Biol] 2021 Oct 06; Vol. 21 (1), pp. 456. Date of Electronic Publication: 2021 Oct 06.
Typ publikacji:
Comparative Study; Journal Article
MeSH Terms:
Cloning, Molecular*
Phenotype*
Promoter Regions, Genetic*
Brassica/*anatomy & histology
Brassica/*genetics
Plant Leaves/*anatomy & histology
Plant Leaves/*genetics
Gene Expression Regulation, Plant ; Genes, Plant ; Genetic Variation ; Genotype
Czasopismo naukowe
Tytuł:
An integrated microfluidics platform with high-throughput single-cell cloning array and concentration gradient generator for efficient cancer drug effect screening.
Autorzy:
Wang B; Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences, Wuhan, 430071, China.
He BS; Department of Laboratory Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, 210006, China. .
Ruan XL; Department of Hematology, Renmin Hospital, Wuhan University, Wuhan, 430060, China.
Zhu J; Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences, Wuhan, 430071, China.; University of Chinese Academy of Sciences, Beijing, 10049, China.
Hu R; Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences, Wuhan, 430071, China.; University of Chinese Academy of Sciences, Beijing, 10049, China.
Wang J; Canary Center at Stanford for Cancer Early Detection, Department of Radiology, School of Medicine, Stanford University, Palo Alto, CA, 94304, USA.
Li Y; Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences, Wuhan, 430071, China. .; University of Chinese Academy of Sciences, Beijing, 10049, China. .
Yang YH; Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences, Wuhan, 430071, China.; University of Chinese Academy of Sciences, Beijing, 10049, China.
Liu ML; Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology-Wuhan National Laboratory for Optoelectronics, Chinese Academy of Sciences, Wuhan, 430071, China.; University of Chinese Academy of Sciences, Beijing, 10049, China.
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Źródło:
Military Medical Research [Mil Med Res] 2022 Sep 22; Vol. 9 (1), pp. 51. Date of Electronic Publication: 2022 Sep 22.
Typ publikacji:
Journal Article
MeSH Terms:
Leukemia*
Microfluidics*/methods
Clone Cells ; Cloning, Molecular ; Early Detection of Cancer ; Humans ; Imatinib Mesylate ; Resveratrol
Czasopismo naukowe
Tytuł:
High-throughput FastCloning technology: A low-cost method for parallel cloning.
Autorzy:
Jiang H; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, P.R. China.
Meng F; Jujing-Chengdu Biotech, Chengdu, Sichuan, P.R. China.
Lu D; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, P.R. China.
Chen Y; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, P.R. China.
Luo G; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, P.R. China.
Chen Y; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, P.R. China.
Chen J; Jujing-Chengdu Biotech, Chengdu, Sichuan, P.R. China.
Chen C; Department of Gynecology and Obstetrics, Chongqing General Hospital, University of Chinese Academy of Sciences, Chongqing, P.R. China.
Zhang X; School of Mechatronic Engineering and Automation, Shanghai University, Shanghai, P.R. China.
Su D; State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan, P.R. China.; Tianjin International Joint Academy of Biotechnology and Medicine, Tianjin, P.R. China.
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Źródło:
PloS one [PLoS One] 2022 Sep 09; Vol. 17 (9), pp. e0273873. Date of Electronic Publication: 2022 Sep 09 (Print Publication: 2022).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Escherichia coli*/genetics
Genetic Vectors*/genetics
Cloning, Molecular ; Plasmids/genetics ; Polymerase Chain Reaction/methods
Czasopismo naukowe
Tytuł:
Cloning and Functional Characterization of a Novel β-GRP Gene From Melanotus cribricollis.
Autorzy:
Ye B; Zhejiang Academy of Forestry, Hangzhou 310023, China.
Song Q; Zhejiang Academy of Forestry, Hangzhou 310023, China.
Li H; Zhejiang Academy of Forestry, Hangzhou 310023, China.
Shen J; Zhejiang Academy of Forestry, Hangzhou 310023, China.
Wu C; Zhejiang Academy of Forestry, Hangzhou 310023, China.
Shu J; Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
Zhang Y; Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China.
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Źródło:
Journal of insect science (Online) [J Insect Sci] 2022 Sep 01; Vol. 22 (5).
Typ publikacji:
Journal Article
MeSH Terms:
Coleoptera*/genetics
Glucans*
Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; DNA, Complementary/genetics ; Phylogeny
Czasopismo naukowe
Tytuł:
Cloning and molecular properties of a novel luciferase from the Brazilian Bicellonycha lividipennis (Lampyridae: Photurinae) firefly: comparison with other firefly luciferases.
Autorzy:
Moreira AC; Department Physics, Chemistry and Mathematics (DFQM), Federal University of São Carlos (UFSCar), Rod. João Leme dos Santos-SP 264-Km 110-Itinga, Sorocaba, São Carlos, SP, Brazil.
Amaral DT; Programa de Pós Graduação em Biologia Comparada, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil.
Gabriel GVM; Department Physics, Chemistry and Mathematics (DFQM), Federal University of São Carlos (UFSCar), Rod. João Leme dos Santos-SP 264-Km 110-Itinga, Sorocaba, São Carlos, SP, Brazil.
Viviani VR; Department Physics, Chemistry and Mathematics (DFQM), Federal University of São Carlos (UFSCar), Rod. João Leme dos Santos-SP 264-Km 110-Itinga, Sorocaba, São Carlos, SP, Brazil. .
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Źródło:
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2022 Sep; Vol. 21 (9), pp. 1559-1571. Date of Electronic Publication: 2022 May 19.
Typ publikacji:
Journal Article
MeSH Terms:
Fireflies*/genetics
Luciferases, Firefly*/chemistry
Luciferases, Firefly*/genetics
Animals ; Brazil ; Cloning, Molecular ; Luciferases/chemistry ; Phylogeny
Czasopismo naukowe
Tytuł:
Molecular cloning, expression and generation of a polyclonal antibody specific for RNPS1.
Autorzy:
Deka B; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, 781039, Guwahati, Assam, India.
Singh KK; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, 781039, Guwahati, Assam, India. .
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Źródło:
Molecular biology reports [Mol Biol Rep] 2022 Sep; Vol. 49 (9), pp. 9095-9100. Date of Electronic Publication: 2022 Aug 08.
Typ publikacji:
Journal Article
MeSH Terms:
RNA Splicing*
RNA-Binding Proteins*/genetics
Animals ; Antibodies ; Cloning, Molecular ; Enzyme-Linked Immunosorbent Assay ; Humans ; Mice ; RNA Splice Sites ; Rabbits ; Rats ; Recombinant Proteins/metabolism ; Ribonucleoproteins/genetics ; Ribonucleoproteins/metabolism
Czasopismo naukowe

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