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Tytuł :
Development of one-pot single specific primer-LAMP (SSP-LAMP) for identification of Shigella genus using 16S rDNA.
Autorzy :
Mollasalehi H; Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, 1983969411, Iran. Electronic address: .
Vahedipour N; Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, 1983969411, Iran.
Taghvamanesh A; Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, 1983969411, Iran.
Minai-Tehrani D; Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, 1983969411, Iran.
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Źródło :
Analytical biochemistry [Anal Biochem] 2022 Jan 01; Vol. 636, pp. 114452. Date of Electronic Publication: 2021 Nov 09.
Typ publikacji :
Journal Article
MeSH Terms :
Bacterial Typing Techniques*
DNA Primers*/chemistry
DNA Primers*/genetics
Molecular Diagnostic Techniques*
Nucleic Acid Amplification Techniques*
Shigella*/classification
Shigella*/genetics
DNA, Bacterial/*genetics
DNA, Ribosomal/*genetics
RNA, Ribosomal, 16S/*genetics
SCR Protocol :
LAMP assay
Czasopismo naukowe
Tytuł :
Synthetic DNA spike-ins (SDSIs) enable sample tracking and detection of inter-sample contamination in SARS-CoV-2 sequencing workflows.
Autorzy :
Lagerborg KA; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Harvard Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA, USA.
Normandin E; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
Bauer MR; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Harvard Program in Biological and Biomedical Sciences, Harvard Medical School, Boston, MA, USA.
Adams G; Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Figueroa K; Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Loreth C; Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Gladden-Young A; Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Shaw BM; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
Pearlman LR; Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Berenzy D; The Jackson Laboratory, Bar Harbor, ME, USA.
Dewey HB; The Jackson Laboratory, Bar Harbor, ME, USA.
Kales S; The Jackson Laboratory, Bar Harbor, ME, USA.
Dobbins ST; Broad Institute of Harvard and MIT, Cambridge, MA, USA.
Shenoy ES; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
Hooper D; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
Pierce VM; Department of Pathology, Massachusetts General Hospital, Boston, MA, USA.; Pediatric Infectious Disease Unit, MassGeneral Hospital for Children, Boston, MA, USA.; Department of Pathology, Harvard Medical School, Boston, MA, USA.
Zachary KC; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.; Department of Medicine, Harvard Medical School, Boston, MA, USA.; Infection Control Unit, Massachusetts General Hospital, Boston, MA, USA.
Park DJ; Broad Institute of Harvard and MIT, Cambridge, MA, USA.
MacInnis BL; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Harvard University, Boston, MA, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA.
Tewhey R; The Jackson Laboratory, Bar Harbor, ME, USA.; Graduate School of Biomedical Sciences and Engineering, University of Maine, Orono, ME, USA.; Graduate School of Biomedical Sciences, Tufts University School of Medicine, Boston, MA, USA.
Lemieux JE; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA.
Sabeti PC; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Systems Biology, Harvard Medical School, Boston, MA, USA.; Department of Immunology and Infectious Diseases, Harvard T. H. Chan School of Public Health, Harvard University, Boston, MA, USA.; Massachusetts Consortium on Pathogen Readiness, Boston, MA, USA.; Howard Hughes Medical Institute, Chevy Chase, MD, USA.
Reilly SK; Broad Institute of Harvard and MIT, Cambridge, MA, USA. .; Department of Genetics, Yale School of Medicine, New Haven, CT, USA. .
Siddle KJ; Broad Institute of Harvard and MIT, Cambridge, MA, USA.; Department of Systems Biology, Harvard Medical School, Boston, MA, USA.
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Źródło :
Nature microbiology [Nat Microbiol] 2022 Jan; Vol. 7 (1), pp. 108-119. Date of Electronic Publication: 2021 Dec 14.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
MeSH Terms :
DNA Primers/*standards
SARS-CoV-2/*genetics
Sequence Analysis/*standards
COVID-19/diagnosis ; DNA Primers/chemical synthesis ; Genome, Viral/genetics ; Humans ; Quality Control ; RNA, Viral/genetics ; Reproducibility of Results ; Sequence Analysis/methods ; Whole Genome Sequencing ; Workflow
Czasopismo naukowe
Tytuł :
Kinetics of protein-assisted nucleic acid interconversion monitored by transient time resolved fluorescence in microfluidic droplets.
Autorzy :
Grytsyk N; Institut de Physique et Chimie des Matériaux de Strasbourg, Université de Strasbourg & CNRS, 67034 Strasbourg, France.; Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de Pharmacie, 67401 Illkirch, France.
Cianfarani D; Institut de Physique et Chimie des Matériaux de Strasbourg, Université de Strasbourg & CNRS, 67034 Strasbourg, France.
Crégut O; Institut de Physique et Chimie des Matériaux de Strasbourg, Université de Strasbourg & CNRS, 67034 Strasbourg, France.
Richert L; Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de Pharmacie, 67401 Illkirch, France.
Boudier C; Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de Pharmacie, 67401 Illkirch, France.
Humbert N; Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de Pharmacie, 67401 Illkirch, France.
Didier P; Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de Pharmacie, 67401 Illkirch, France.
Mély Y; Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Faculté de Pharmacie, 67401 Illkirch, France.
Léonard J; Institut de Physique et Chimie des Matériaux de Strasbourg, Université de Strasbourg & CNRS, 67034 Strasbourg, France.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2021 Nov 08; Vol. 49 (19), pp. e111.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
DNA Primers/*chemistry
HIV-1/*chemistry
Molecular Chaperones/*chemistry
Nucleocapsid Proteins/*chemistry
Peptides/*chemistry
Serum Albumin, Human/*chemistry
Base Pairing ; DNA Primers/metabolism ; Fluoresceins/chemistry ; Fluorescence ; Fluorescence Resonance Energy Transfer ; HIV-1/metabolism ; Humans ; Kinetics ; Microfluidic Analytical Techniques ; Molecular Chaperones/metabolism ; Nucleic Acid Conformation ; Nucleocapsid Proteins/metabolism ; Peptides/metabolism ; Serum Albumin, Human/metabolism ; p-Dimethylaminoazobenzene/analogs & derivatives ; p-Dimethylaminoazobenzene/chemistry
Czasopismo naukowe
Tytuł :
Developing a new genic SSR primer database in faba bean (Vicia faba L.).
Autorzy :
Khalifa KA; Agricultural Genetic Engineering Research Institute, PO Box 12619, Giza, Egypt.
Ibrahim SD; Agricultural Genetic Engineering Research Institute, PO Box 12619, Giza, Egypt.
El-Garhy HAS; Department of Genetics and Genetic Engineering, Faculty of Agricultural, Benha University, Benha, Egypt.
Moustafa MMM; Department of Genetics and Genetic Engineering, Faculty of Agricultural, Benha University, Benha, Egypt.
Maalouf F; International Center for Agricultural Research in the Dry Areas (ICARDA) , Terbol, Lebanon.
Alsamman AM; Agricultural Genetic Engineering Research Institute, PO Box 12619, Giza, Egypt.
Hamwieh A; International Center for Agricultural Research in the Dry Areas (ICARDA), PO Box 2416, Cairo, Egypt.
El Allali A; African Genome Center, Mohammed VI Polytechnic University, Ben Guerir, Morocco. .
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Źródło :
Journal of applied genetics [J Appl Genet] 2021 Sep; Vol. 62 (3), pp. 373-387. Date of Electronic Publication: 2021 Mar 23.
Typ publikacji :
Journal Article
MeSH Terms :
DNA Primers*
Databases, Genetic*
Microsatellite Repeats*
Vicia faba*/genetics
Expressed Sequence Tags ; Genotype ; Polymorphism, Genetic
Czasopismo naukowe
Tytuł :
CoVrimer: A tool for aligning SARS-CoV-2 primer sequences and selection of conserved/degenerate primers.
Autorzy :
Vural-Ozdeniz M; Interdisciplinary Neuroscience Program, Bilkent University, Ankara, Turkey.
Akturk A; Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.
Demirdizen M; Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.
Leka R; Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.
Acar R; Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey.
Konu O; Interdisciplinary Neuroscience Program, Bilkent University, Ankara, Turkey; Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey. Electronic address: .
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Źródło :
Genomics [Genomics] 2021 Sep; Vol. 113 (5), pp. 3174-3184. Date of Electronic Publication: 2021 Jul 20.
Typ publikacji :
Journal Article
MeSH Terms :
Software*
DNA Primers/*chemistry
SARS-CoV-2/*genetics
Sequence Analysis, DNA/*methods
Conserved Sequence ; DNA Primers/genetics ; Genome, Viral ; Mutation
Czasopismo naukowe
Tytuł :
A novel method for conserved sequence extraction with prospective mutation prediction for SARS-CoV-2 PCR primer design.
Autorzy :
Portakal SH; Izmir University of Economics, Faculty of Engineering, Department of Biomedical Engineering, Izmir, Turkey.
Kanat B; Izmir University of Economics, Faculty of Engineering, Department of Biomedical Engineering, Izmir, Turkey.
Sayan M; Kocaeli University, Faculty of Medicine, Clinical Laboratory, PCR Unit, Kocaeli, Turkey; Turkish Republic of Northern Cyprus.
Berber B; Eskisehir Technical University, Faculty of Science, Department of Biology, Eskisehir, Turkey.
Doluca O; Izmir University of Economics, Faculty of Engineering, Department of Biomedical Engineering, Izmir, Turkey. Electronic address: .
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Źródło :
Journal of virological methods [J Virol Methods] 2021 Jul; Vol. 293, pp. 114146. Date of Electronic Publication: 2021 Apr 01.
Typ publikacji :
Journal Article
MeSH Terms :
DNA Primers*
Mutation*
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
SARS-CoV-2/*genetics
Algorithms ; Humans ; Prospective Studies ; Sequence Alignment
Czasopismo naukowe
Tytuł :
Genotyping of Human Platelet Antigen-1 to -5 and -15 by Polymerase Chain Reaction with Sequence-specific Primers (PCR-SSP) and Real-time PCR in Azeri Blood Donors.
Autorzy :
Azizi SG; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. .
Samiee S; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. .
Shaiegan M; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. .
Zadsar M; Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran. .
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Źródło :
Iranian journal of allergy, asthma, and immunology [Iran J Allergy Asthma Immunol] 2021 Jun 06; Vol. 20 (3), pp. 350-363. Date of Electronic Publication: 2021 Jun 06.
Typ publikacji :
Comparative Study; Journal Article
MeSH Terms :
Blood Donors*
Blood Grouping and Crossmatching*
DNA Primers*
Real-Time Polymerase Chain Reaction*
Antigens, Human Platelet/*genetics
Blood Platelets/*immunology
Blood Transfusion ; Gene Frequency ; Genotype ; Histocompatibility ; Humans ; Iran ; Predictive Value of Tests
Czasopismo naukowe
Tytuł :
The SOS Error-Prone DNA Polymerase V Mutasome and β-Sliding Clamp Acting in Concert on Undamaged DNA and during Translesion Synthesis.
Autorzy :
Sikand A; Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.
Jaszczur M; Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA.
Bloom LB; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32611, USA.
Woodgate R; Laboratory of Genomic Integrity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20814, USA.
Cox MM; Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Goodman MF; Department of Chemistry, University of Southern California, Los Angeles, CA 90089, USA.; Department of Biological Sciences, University of Southern California, Los Angeles, CA 90089, USA.
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Źródło :
Cells [Cells] 2021 May 01; Vol. 10 (5). Date of Electronic Publication: 2021 May 01.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
DNA Primers*
DNA/*analysis
DNA-Directed DNA Polymerase/*genetics
DNA/genetics ; DNA/metabolism ; DNA Damage ; DNA Repair ; DNA Replication ; DNA-Directed DNA Polymerase/metabolism ; Escherichia coli/metabolism ; Escherichia coli Proteins/chemistry ; Mutagenesis ; Mutation ; Plasmids/metabolism ; Ultraviolet Rays
Czasopismo naukowe
Tytuł :
DNA barcodes and new primers for nature's pest controllers: the social wasps.
Autorzy :
Onah IE; Department of Zoology and Environmental Biology, University of Nigeria, 410001 Nsukka, Enugu State, Nigeria.; Centre for Biodiversity and Environment Research, University College London, London WC1E 6BT, UK.
Sumner S; Centre for Biodiversity and Environment Research, University College London, London WC1E 6BT, UK.
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Źródło :
Genome [Genome] 2021 May; Vol. 64 (5), pp. 581-590. Date of Electronic Publication: 2020 Nov 10.
Typ publikacji :
Journal Article
MeSH Terms :
DNA Primers*
Pest Control*
DNA Barcoding, Taxonomic/*methods
Wasps/*genetics
Animals ; Biodiversity ; DNA/analysis ; Insecta/genetics ; Phylogeny ; Polymerase Chain Reaction ; Wasps/classification
Czasopismo naukowe
Tytuł :
SYBR Green real-time qPCR method: Diagnose drowning more rapidly and accurately.
Autorzy :
Yu Z; School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China.
Xu Q; Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China.
Xiao C; Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China.
Li H; Department of Forensic Medicine, Faculty of Basic Medical Sciences, Chongqing Medical University, Chongqing 400016, China.
Wu W; HOMY GeneTech Incorporation, Foshan 518000, China.
Du W; HOMY GeneTech Incorporation, Foshan 518000, China.
Zhao J; Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China.
Liu H; Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China.
Wang H; School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China. Electronic address: .
Liu C; School of Forensic Medicine, Southern Medical University, Guangzhou 510515, China; Guangzhou Forensic Science Institute, Guangdong Province Key Laboratory of Forensic Genetics, Guangzhou 510030, China. Electronic address: .
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Źródło :
Forensic science international [Forensic Sci Int] 2021 Apr; Vol. 321, pp. 110720. Date of Electronic Publication: 2021 Feb 11.
Typ publikacji :
Journal Article
MeSH Terms :
DNA Primers*
Aeromonas/*genetics
Cyanobacteria/*genetics
Diatoms/*genetics
Drowning/*diagnosis
Benzothiazoles ; Diamines ; Fluorescent Dyes ; Humans ; Kidney/pathology ; Liver/pathology ; Lung/pathology ; Quinolines ; Real-Time Polymerase Chain Reaction
Czasopismo naukowe
Tytuł :
barCoder: a tool to generate unique, orthogonal genetic tags for qPCR detection.
Autorzy :
Bernhards CB; U.S. Army Combat Capabilities Development Command Chemical Biological Center, Aberdeen Proving Ground, MD, 21010, USA.; Excet, Inc., Springfield, VA, 22150, USA.
Lux MW; U.S. Army Combat Capabilities Development Command Chemical Biological Center, Aberdeen Proving Ground, MD, 21010, USA.
Katoski SE; U.S. Army Combat Capabilities Development Command Chemical Biological Center, Aberdeen Proving Ground, MD, 21010, USA.
Goralski TDP; U.S. Army Combat Capabilities Development Command Chemical Biological Center, Aberdeen Proving Ground, MD, 21010, USA.
Liem AT; U.S. Army Combat Capabilities Development Command Chemical Biological Center, Aberdeen Proving Ground, MD, 21010, USA.; DCS Corporation, Abingdon, MD, 21009, USA.
Gibbons HS; U.S. Army Combat Capabilities Development Command Chemical Biological Center, Aberdeen Proving Ground, MD, 21010, USA. .
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Źródło :
BMC bioinformatics [BMC Bioinformatics] 2021 Mar 01; Vol. 22 (1), pp. 98. Date of Electronic Publication: 2021 Mar 01.
Typ publikacji :
Journal Article
MeSH Terms :
Bacillus anthracis*/genetics
DNA Barcoding, Taxonomic*
DNA Primers*
Algorithms ; Genome ; Real-Time Polymerase Chain Reaction
Czasopismo naukowe
Tytuł :
An adaptable dry lab for SYBR based RT-qPCR primer design to reinforce concepts in molecular biology and nucleic acids.
Autorzy :
Covey SD; Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada.
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Źródło :
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology [Biochem Mol Biol Educ] 2021 Mar; Vol. 49 (2), pp. 262-270. Date of Electronic Publication: 2020 Sep 08.
Typ publikacji :
Journal Article
MeSH Terms :
Real-Time Polymerase Chain Reaction*
Computational Biology/*education
DNA Primers/*chemistry
Molecular Biology/*education
DNA Primers/genetics ; Humans ; Laboratories ; Learning ; Students
Czasopismo naukowe
Tytuł :
PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens.
Autorzy :
MacLeod IJ; Department of Immunology and Infectious Diseases, Harvard TH Chan School of Public Health, Boston, MA, USA. .; Botswana-Harvard AIDS Institute Partnership, Private Bag, Gaborone, Botswana. .
Rowley CF; Department of Immunology and Infectious Diseases, Harvard TH Chan School of Public Health, Boston, MA, USA.; Botswana-Harvard AIDS Institute Partnership, Private Bag, Gaborone, Botswana.; Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, MA, USA.
Essex M; Department of Immunology and Infectious Diseases, Harvard TH Chan School of Public Health, Boston, MA, USA.; Botswana-Harvard AIDS Institute Partnership, Private Bag, Gaborone, Botswana.
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Źródło :
Communications biology [Commun Biol] 2021 Feb 18; Vol. 4 (1), pp. 227. Date of Electronic Publication: 2021 Feb 18.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural
MeSH Terms :
DNA Primers*
Point Mutation*
Polymerase Chain Reaction*
DNA, Viral/*genetics
Drug Resistance, Viral/*genetics
HIV Infections/*virology
HIV-1/*genetics
Virology/*methods
Anti-HIV Agents/therapeutic use ; Efavirenz, Emtricitabine, Tenofovir Disoproxil Fumarate Drug Combination/therapeutic use ; Genotype ; HIV Infections/diagnosis ; HIV Infections/drug therapy ; HIV-1/drug effects ; High-Throughput Nucleotide Sequencing ; Humans ; Reproducibility of Results
Czasopismo naukowe
Tytuł :
A modified loop-mediated isothermal amplification method for detecting avian infectious laryngotracheitis virus.
Autorzy :
Yu, Jifeng (AUTHOR)
Xie, Jing (AUTHOR)
Cao, Ye (AUTHOR)
Kang, Runmin (AUTHOR)
Lin, Yi (AUTHOR)
Wei, Yong (AUTHOR)
Liao, Dangjin (AUTHOR)
Li, Xingyu (AUTHOR)
Ye, Yonggang (AUTHOR)
Pan, Meng (AUTHOR)
Xiao, Lu (AUTHOR)
Ye, Jianqiang (AUTHOR)
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Źródło :
Animal Biotechnology. Dec 2021, Vol. 32 Issue 6, p766-773. 8p.
Czasopismo naukowe
Tytuł :
Defining genome-wide CRISPR-Cas genome-editing nuclease activity with GUIDE-seq.
Autorzy :
Malinin NL; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Lee G; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Lazzarotto CR; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Li Y; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Zheng Z; Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.
Nguyen NT; Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.; Cutaneous Biology Research Center, Department of Dermatology, Massachusetts General Hospital, and Harvard Medical School, Boston, MA, USA.
Liebers M; Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.
Topkar VV; Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.; Biophysics Program, Stanford University, Stanford, CA, USA.
Iafrate AJ; Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.
Le LP; Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.
Aryee MJ; Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.
Joung JK; Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.
Tsai SQ; Department of Hematology, St. Jude Children's Research Hospital, Memphis, TN, USA. .
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Źródło :
Nature protocols [Nat Protoc] 2021 Dec; Vol. 16 (12), pp. 5592-5615. Date of Electronic Publication: 2021 Nov 12.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
CRISPR-Cas Systems*
Genome, Human*
CRISPR-Associated Protein 9/*genetics
Gene Editing/*methods
Polymerase Chain Reaction/*methods
RNA, Guide/*genetics
CRISPR-Associated Protein 9/metabolism ; Cell Line, Tumor ; Clustered Regularly Interspaced Short Palindromic Repeats ; DNA Primers/chemical synthesis ; DNA Primers/metabolism ; Deoxyribonucleases, Type II Site-Specific/chemistry ; Electroporation/methods ; Humans ; Osteoblasts/cytology ; Osteoblasts/metabolism ; Plasmids/chemistry ; Plasmids/metabolism ; Primary Cell Culture ; RNA, Guide/metabolism ; T-Lymphocytes/cytology ; T-Lymphocytes/metabolism
Czasopismo naukowe
Tytuł :
A DNA intercalating dye-based RT-qPCR alternative to diagnose SARS-CoV-2.
Autorzy :
Fuchs Wightman F; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Godoy Herz MA; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Muñoz JC; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Stigliano JN; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Bragado L; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Moreno NN; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Palavecino M; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Servi L; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Cabrerizo G; Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires, Buenos Aires, Argentina.
Clemente J; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Avaro M; National Influenza Centre PAHO/WHO, Servicio Virosis Respiratorias, Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
Pontoriero A; National Influenza Centre PAHO/WHO, Servicio Virosis Respiratorias, Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
Benedetti E; National Influenza Centre PAHO/WHO, Servicio Virosis Respiratorias, Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
Baumeister E; National Influenza Centre PAHO/WHO, Servicio Virosis Respiratorias, Departamento Virología, Instituto Nacional de Enfermedades Infecciosas, Buenos Aires, Argentina.
Rudolf F; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland.; SIB Swiss Institute of Bioinformatics, ETH Zurich, Basel, Switzerland.
Remes Lenicov F; Instituto de Investigaciones Biomédicas en Retrovirus y SIDA (INBIRS), Universidad de Buenos Aires, Buenos Aires, Argentina.
Garcia C; Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Universidad De Buenos Aires, Buenos Aires, Argentina.; Laboratorio de Estrategias Antivirales- IQUIBICEN, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.
Buggiano V; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Kornblihtt AR; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Srebrow A; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
de la Mata M; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Muñoz MJ; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Fondazione Istituto FIRC di Oncologia Molecolare (IFOM), Milan, Italy.; Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.
Schor IE; Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.
Petrillo E; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-UBA-CONICET), Ciudad Universitaria, Buenos Aires, Argentina.; Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, Argentina.; Instituto Multidisciplinario de Investigación en Patologías Pediátricas (IMIPP), CONICET - GCBA, Buenos Aires, Argentina.
Pokaż więcej
Źródło :
RNA biology [RNA Biol] 2021 Dec; Vol. 18 (12), pp. 2218-2225. Date of Electronic Publication: 2021 May 26.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Benzothiazoles/*chemistry
COVID-19/*diagnosis
COVID-19 Nucleic Acid Testing/*methods
Diamines/*chemistry
Intercalating Agents/*chemistry
Quinolines/*chemistry
RNA, Viral/*genetics
Real-Time Polymerase Chain Reaction/*methods
SARS-CoV-2/*genetics
COVID-19/virology ; COVID-19 Nucleic Acid Testing/standards ; DNA/analysis ; DNA/biosynthesis ; DNA Primers/chemistry ; DNA Primers/metabolism ; Humans ; Nasopharynx/virology ; Real-Time Polymerase Chain Reaction/standards ; Sensitivity and Specificity
Czasopismo naukowe
Tytuł :
Rolling circle amplification (RCA)-based DNA hydrogel.
Autorzy :
Yao C; Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, P. R. China.
Zhang R; Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, P. R. China.
Tang J; Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, P. R. China.
Yang D; Frontiers Science Center for Synthetic Biology, Key Laboratory of Systems Bioengineering (MOE), School of Chemical Engineering and Technology, Tianjin University, Tianjin, P. R. China. .
Pokaż więcej
Źródło :
Nature protocols [Nat Protoc] 2021 Dec; Vol. 16 (12), pp. 5460-5483. Date of Electronic Publication: 2021 Oct 29.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't; Review
MeSH Terms :
Aptamers, Nucleotide/*metabolism
DNA, Circular/*chemistry
DNA, Single-Stranded/*chemistry
Hydrogels/*chemistry
Nucleic Acid Amplification Techniques/*standards
Polymerase Chain Reaction/*methods
Animals ; Aptamers, Nucleotide/chemical synthesis ; Bone Marrow Cells/cytology ; Bone Marrow Cells/metabolism ; Cell Separation/methods ; DNA Primers/chemical synthesis ; DNA Primers/metabolism ; DNA, Circular/genetics ; DNA, Circular/metabolism ; DNA, Single-Stranded/genetics ; DNA, Single-Stranded/metabolism ; DNA-Directed DNA Polymerase/genetics ; DNA-Directed DNA Polymerase/metabolism ; Humans ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Mice ; Nucleic Acid Amplification Techniques/instrumentation ; Nucleic Acid Hybridization/methods ; Viral Proteins/genetics ; Viral Proteins/metabolism
Czasopismo naukowe

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