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Wyświetlanie 61-80 z 27431
Tytuł:
Three novel multiplex PCR assays for rapid detection of virulence, antimicrobial resistance, and toxin genes in Acinetobacter calcoaceticus-baumannii complex species.
Autorzy:
Sheikh, Alexander A (AUTHOR)
Schneiderman, Danielle (AUTHOR)
Sykes, Ellen M E (AUTHOR)
Kumar, Ayush (AUTHOR)
Chen, Wen (AUTHOR)
Lapen, David R (AUTHOR)
Khan, Izhar U H (AUTHOR)
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Źródło:
Letters in Applied Microbiology. Mar2024, Vol. 77 Issue 3, p1-9. 9p.
Czasopismo naukowe
Szczegóły
Tytuł:
Design and validation of Dolosigranulum pigrum specific PCR primers using the bacterial core genome.
Autorzy:
Aziz M; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
Palmer A; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
Iversen S; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
Salazar JE; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
Pham T; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
Roach K; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
Becker K; Friedrich Loeffler-Institute of Medical Microbiology, University Medicine Greifswald, Greifswald, Germany.
Kaspar U; Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.
Price LB; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA.
Baig S; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
Stegger M; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
Andersen PS; Department of Bacteria, Parasites, and Fungi, Statens Serum Institut, Artillerivej 5, 2300, Copenhagen, Denmark.
Liu CM; Antibiotic Resistance Action Center, Department of Environmental and Occupational Health, Milken Institute School of Public Health, George Washington University, 800 22nd Street NW, Washington, DC, 20052, USA. .
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Źródło:
Scientific reports [Sci Rep] 2023 Apr 14; Vol. 13 (1), pp. 6110. Date of Electronic Publication: 2023 Apr 14.
Typ publikacji:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms:
Gram-Positive Bacterial Infections*/microbiology
Gram-Positive Cocci*
Humans ; RNA, Ribosomal, 16S/genetics ; Polymerase Chain Reaction ; DNA Primers ; DNA, Bacterial
SCR Organism:
Dolosigranulum pigrum
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
A highly efficient and accurate method of detecting and subtyping Influenza A pdm H1N1 and H3N2 viruses with newly emerging mutations in the matrix gene in Eastern Taiwan.
Autorzy:
Yang HH; Bioinnovation Center, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; Department of Medical Research, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; Taiwan CDC Collaborating Laboratories of Virology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
Huang IT; Taiwan CDC Collaborating Laboratories of Virology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; Department of Laboratory Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.
Wu RC; Branch of Clinical Pathology, Department of Laboratory Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; Department of Emergency Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; PhD Program in Pharmacology and Toxicology, Tzu Chi University, Hualien, Taiwan.
Chen LK; Taiwan CDC Collaborating Laboratories of Virology, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; Department of Laboratory Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; Branch of Clinical Pathology, Department of Laboratory Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; Department of Emergency Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; PhD Program in Pharmacology and Toxicology, Tzu Chi University, Hualien, Taiwan.; Institute of Medical Sciences, Department of Laboratory Diagnostic, College of Medicine, Tzu Chi University, Hualien, Taiwan.
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Źródło:
PloS one [PLoS One] 2023 Mar 23; Vol. 18 (3), pp. e0283074. Date of Electronic Publication: 2023 Mar 23 (Print Publication: 2023).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Influenza, Human*/diagnosis
Influenza A Virus, H1N1 Subtype*
Influenza A virus*/genetics
Humans ; Influenza A Virus, H3N2 Subtype ; Taiwan ; Phylogeny ; Sensitivity and Specificity ; Mutation ; DNA Primers/genetics ; Reverse Transcriptase Polymerase Chain Reaction
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Differential coexistence of multiple genotypes of Ophiocordyceps sinensis in the stromata, ascocarps and ascospores of natural Cordyceps sinensis.
Autorzy:
Li YL; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, Qinghai, China.
Li XZ; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, Qinghai, China.
Yao YS; Shenzhen Key Laboratory of Health Science and Technology, Institute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.
Wu ZM; School of Life Sciences, Tsinghua University, Beijing, China.
Gao L; Shenzhen Key Laboratory of Health Science and Technology, Institute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.
Tan NZ; Shenzhen Key Laboratory of Health Science and Technology, Institute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.
Luo ZQ; School of Life Sciences, Tsinghua University, Beijing, China.
Xie WD; Shenzhen Key Laboratory of Health Science and Technology, Institute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.
Wu JY; State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Shenzhen, Guangdong, China.; Department of Applied Biology and Chemistry Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
Zhu JS; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai Academy of Animal and Veterinary Sciences, Qinghai University, Xining, Qinghai, China.; Shenzhen Key Laboratory of Health Science and Technology, Institute of Biopharmaceutical and Health Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen, China.
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Źródło:
PloS one [PLoS One] 2023 Mar 09; Vol. 18 (3), pp. e0270776. Date of Electronic Publication: 2023 Mar 09 (Print Publication: 2023).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cordyceps*/genetics
Bayes Theorem ; DNA ; DNA Primers/genetics ; Genotype
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Electrochemical detection of the p53 gene using exponential amplification reaction (EXPAR) and CRISPR/Cas12a reactions.
Autorzy:
Zhou S; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, People's Republic of China.
Deng L; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, People's Republic of China.
Dong J; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, People's Republic of China.
Lu P; Chongqing University, Three Gorges Hospital, Chongqing, 404000, People's Republic of China.
Qi N; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, People's Republic of China.
Huang Z; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, SeNA Research Institute and Szostak-CDHT Large Nucleic Acids Institute, Chengdu, 610065, People's Republic of China.
Yang M; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, People's Republic of China.
Huo D; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, People's Republic of China. .
Hou C; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, People's Republic of China. .; Chongqing Key Laboratory of Bio-Perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, 400044, People's Republic of China. .
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Źródło:
Mikrochimica acta [Mikrochim Acta] 2023 Mar 04; Vol. 190 (4), pp. 113. Date of Electronic Publication: 2023 Mar 04.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Cas Systems*
Genes, p53*
Humans ; DNA Primers ; Electrodes ; Fluorescence
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Vivid COVID-19 LAMP is an ultrasensitive, quadruplexed test using LNA-modified primers and a zinc ion and 5-Br-PAPS colorimetric detection system.
Autorzy:
Szobi A; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Buranovská K; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Vojtaššáková N; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Lovíšek D; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Özbaşak HÖ; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Szeibeczederová S; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Kapustian L; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Hudáčová Z; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.; Stanford University, 730 Escondido Rd., Stanford, CA, 94305, USA.
Kováčová V; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.; University of Cologne, Institute for Biological Physics, Zülpicher Str. 77, 50937, Köln, Germany.
Drobná D; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Putaj P; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Bírová S; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Čirková I; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Čarnecký M; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Kilián P; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia.; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA.
Jurkáček P; AstonITM s.r.o., Račianska 153, 831 54, Bratislava, Slovakia.
Čabanová V; Biomedical Research Center, Slovak Academy of Sciences, Institute of Virology, Dúbravská cesta 9, 845 05, Bratislava, Slovakia.
Boršová K; Biomedical Research Center, Slovak Academy of Sciences, Institute of Virology, Dúbravská cesta 9, 845 05, Bratislava, Slovakia.
Sláviková M; Biomedical Research Center, Slovak Academy of Sciences, Institute of Virology, Dúbravská cesta 9, 845 05, Bratislava, Slovakia.
Vaňová V; Biomedical Research Center, Slovak Academy of Sciences, Institute of Virology, Dúbravská cesta 9, 845 05, Bratislava, Slovakia.; Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Ilkovičova 6, 842 15, Bratislava, Slovakia.
Klempa B; Biomedical Research Center, Slovak Academy of Sciences, Institute of Virology, Dúbravská cesta 9, 845 05, Bratislava, Slovakia.; Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Ilkovičova 6, 842 15, Bratislava, Slovakia.
Čekan P; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia. .; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA. .
Paul ED; MultiplexDX, s.r.o., Comenius University Science Park, Ilkovičova 8, 841 04, Bratislava, Slovakia. .; MultiplexDX, Inc., One Research Court, Suite 450, Rockville, MD, 20850, USA. .
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Źródło:
Communications biology [Commun Biol] 2023 Mar 02; Vol. 6 (1), pp. 233. Date of Electronic Publication: 2023 Mar 02.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Zinc*
COVID-19*/diagnosis
Humans ; Colorimetry ; SARS-CoV-2/genetics ; DNA Primers ; Ions
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
A prolific and robust whole-genome genotyping method using PCR amplification via primer-template mismatched annealing.
Autorzy:
Zhao S; National Key Laboratory for Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, China.; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Zhang C; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Wang L; National Key Laboratory for Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, China.
Luo M; National Key Laboratory for Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, China.; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Zhang P; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Wang Y; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Malik WA; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Wang Y; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Chen P; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Qiu X; College of Agriculture, Yangtze University, Jingzhou, 434023, China.
Wang C; Guangdong Provincial Key Laboratory of New Technology in Rice Breeding, Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Lu H; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Xiang Y; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Liu Y; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Ruan J; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Qian Q; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
Zhi H; National Key Laboratory for Crop Genetics and Germplasm Enhancement, National Center for Soybean Improvement, Nanjing Agricultural University, Nanjing, 210095, China.
Chang Y; Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
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Źródło:
Journal of integrative plant biology [J Integr Plant Biol] 2023 Mar; Vol. 65 (3), pp. 633-645. Date of Electronic Publication: 2023 Jan 13.
Typ publikacji:
Journal Article
MeSH Terms:
Genome*
Genotype ; DNA Primers/genetics ; Polymerase Chain Reaction/methods
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
High-throughput primer design by scoring in piecewise logistic model for multiple polymerase chain reaction variants.
Autorzy:
Zeng, Huaping (AUTHOR)
Chen, Kexin (AUTHOR)
Ma, Chouxian (AUTHOR)
Zhu, Biyin (AUTHOR)
Chuan, Jun (AUTHOR)
Zhang, Shuan (AUTHOR)
Tang, Lin (AUTHOR)
Yang, Ting (AUTHOR)
Sun, Zhaohui (AUTHOR)
Yang, Xingkun (AUTHOR)
Wang, Yu (AUTHOR)
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Źródło:
Scientific Reports. 12/7/2022, Vol. 12 Issue 1, p1-14. 14p.
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Complete mitogenome of Treron sphenurus (Aves, Columbiformes): the first representative from the genus Treron, genomic comparisons and phylogenetic analysis of Columbidae.
Autorzy:
Chen, Yun-Xia (AUTHOR)
Sun, Cheng-He (AUTHOR)
Li, Yao-Kai (AUTHOR)
Fei, Yi-Ling (AUTHOR)
Xue, Xiao-Ming (AUTHOR)
Hou, Sen-Lin (AUTHOR)
Zhou, Yong-Wu (AUTHOR)
Jiang, Jing (AUTHOR)
Guo, Hai-Tao (AUTHOR)
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Źródło:
Animal Biotechnology. Nov2022, Vol. 33 Issue 6, p1003-1013. 11p.
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Disentangling primer interactions improves SARS-CoV-2 genome sequencing by multiplex tiling PCR.
Autorzy:
Itokawa K; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan.
Sekizuka T; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan.
Hashino M; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan.
Tanaka R; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan.
Kuroda M; Pathogen Genomics Center, National Institute of Infectious Diseases, Tokyo, Japan.
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Źródło:
PloS one [PLoS One] 2020 Sep 18; Vol. 15 (9), pp. e0239403. Date of Electronic Publication: 2020 Sep 18 (Print Publication: 2020).
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Betacoronavirus/*genetics
DNA Primers/*standards
Genome, Viral/*genetics
Multiplex Polymerase Chain Reaction/*methods
Whole Genome Sequencing/*methods
COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Clinical Laboratory Techniques/standards ; Coronavirus Infections/diagnosis ; DNA Primers/metabolism ; Dimerization ; Gene Amplification ; Humans ; Nucleic Acid Amplification Techniques/methods ; SARS-CoV-2 ; Viral Load
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
AutoCloner: automatic homologue-specific primer design for full-gene cloning in polyploids.
Autorzy:
Coulton A; Biological Sciences Department, The University of Bristol, 24 Tyndall Avenue, Bristol, BS8 1TQ, UK. .
Edwards KJ; Biological Sciences Department, The University of Bristol, 24 Tyndall Avenue, Bristol, BS8 1TQ, UK.
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Źródło:
BMC bioinformatics [BMC Bioinformatics] 2020 Jul 16; Vol. 21 (1), pp. 311. Date of Electronic Publication: 2020 Jul 16.
Typ publikacji:
Journal Article
MeSH Terms:
Cloning, Molecular*
Polyploidy*
Sequence Homology*
Software*
Computational Biology/*methods
DNA Primers/*metabolism
Triticum/*genetics
Amino Acid Substitution/genetics ; Base Sequence ; DNA Primers/genetics ; Polymerase Chain Reaction/methods ; Polymorphism, Single Nucleotide/genetics
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Rapid System to Detect Variants of SARS-CoV-2 in Nasopharyngeal Swabs.
Autorzy:
Favaro M; Department Experimental Medicine, University of Rome 'Tor Vergata', 00133 Rome, Italy.
Zampini P; Via Luigi Einaudi, Guidonia Montecelio, 00012 Rome, Italy.
Pistoia ES; Department Experimental Medicine, University of Rome 'Tor Vergata', 00133 Rome, Italy.
Gaziano R; Department Experimental Medicine, University of Rome 'Tor Vergata', 00133 Rome, Italy.
Grelli S; Department Experimental Medicine, University of Rome 'Tor Vergata', 00133 Rome, Italy.
Fontana C; National Institute for Infectious Diseases Lazzaro Spallanzani IRCCS, 00149 Rome, Italy.
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Źródło:
Viruses [Viruses] 2023 Jan 26; Vol. 15 (2). Date of Electronic Publication: 2023 Jan 26.
Typ publikacji:
Journal Article
MeSH Terms:
COVID-19*/diagnosis
Humans ; SARS-CoV-2/genetics ; DNA Primers ; Diffusion ; Nasopharynx
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Improved 18S and 28S rDNA primer sets for NGS-based parasite detection.
Autorzy:
Kounosu A; Division of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, 889-1692, Japan.
Murase K; Division of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, 889-1692, Japan.
Yoshida A; Division of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, 889-1692, Japan.
Maruyama H; Division of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, 889-1692, Japan.
Kikuchi T; Division of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki, 889-1692, Japan. taisei_.
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Źródło:
Scientific reports [Sci Rep] 2019 Oct 31; Vol. 9 (1), pp. 15789. Date of Electronic Publication: 2019 Oct 31.
Typ publikacji:
Journal Article
MeSH Terms:
DNA Primers*/chemistry
DNA Primers*/genetics
Eukaryota*/classification
Eukaryota*/genetics
High-Throughput Nucleotide Sequencing*
Parasites*/classification
Parasites*/genetics
Polymerase Chain Reaction*
RNA, Ribosomal, 16S/*genetics
RNA, Ribosomal, 18S/*genetics
Animals
Czasopismo naukowe
Pełny tekst  Link otwiera się w nowym oknie Pełny tekstSzczegóły
Tytuł:
Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design.
Autorzy:
Püllmann P; Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle (Saale), Germany.
Ulpinnis C; Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle (Saale), Germany.
Marillonnet S; Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle (Saale), Germany.
Gruetzner R; Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle (Saale), Germany.
Neumann S; Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle (Saale), Germany. .; German Centre for Integrative Biodiversity Research (iDiv), Halle-Jena-Leipzig, Deutscher Platz 5e, 04103, Leipzig, Germany. .
Weissenborn MJ; Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120, Halle (Saale), Germany. .; Institute of Chemistry, Martin-Luther-University Halle-Wittenberg, Kurt-Mothes-Str. 2, 06120, Halle (Saale), Germany. .
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Źródło:
Scientific reports [Sci Rep] 2019 Jul 29; Vol. 9 (1), pp. 10932. Date of Electronic Publication: 2019 Jul 29.
Typ publikacji:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Mutagenesis*
Software*
DNA Primers/*genetics
Sequence Analysis, DNA/*methods
Animals ; DNA Primers/chemistry ; DNA Primers/standards ; Humans ; Sequence Analysis, DNA/standards
Czasopismo naukowe
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