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Wyszukujesz frazę ""Electrophoretic Mobility Shift Assay"" wg kryterium: Temat


Tytuł :
FREMSA: A Method That Provides Direct Evidence of the Interaction between microRNA and mRNA.
Autorzy :
Yu D; National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, USA. .; School of Public Health, Qingdao University, Qingdao, China. .
Chen S; National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, USA.
Li D; National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, USA.
Knox B; National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, USA.
Guo L; National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, USA.
Ning B; National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR, USA.
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2102, pp. 557-566.
Typ publikacji :
Journal Article
MeSH Terms :
Electrophoretic Mobility Shift Assay/*methods
MicroRNAs/*metabolism
RNA, Messenger/*metabolism
Cell Line ; Electrophoresis, Polyacrylamide Gel ; Electrophoretic Mobility Shift Assay/instrumentation ; Fluorescence ; Humans ; MicroRNAs/genetics ; Oligonucleotides/chemistry ; Oligonucleotides/genetics ; RNA, Messenger/genetics ; Workflow
Czasopismo naukowe
Tytuł :
Activation of the Aryl Hydrocarbon Receptor (AHR) induces human glutathione S transferase alpha 1 (hGSTA1) expression.
Autorzy :
Martínez-Guzmán C; Department of Cellular Biology, CINVESTAV-IPN, Av. IPN 2508, C.P, 07360, Mexico.
Cortés-Reynosa P; Department of Cellular Biology, CINVESTAV-IPN, Av. IPN 2508, C.P, 07360, Mexico.
Pérez-Salazar E; Department of Cellular Biology, CINVESTAV-IPN, Av. IPN 2508, C.P, 07360, Mexico.
Murillo-González FE; Department of Cellular Biology, CINVESTAV-IPN, Av. IPN 2508, C.P, 07360, Mexico.
Elizondo G; Department of Cellular Biology, CINVESTAV-IPN, Av. IPN 2508, C.P, 07360, Mexico. Electronic address: .
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Źródło :
Chemico-biological interactions [Chem Biol Interact] 2020 Nov 01; Vol. 331, pp. 109284. Date of Electronic Publication: 2020 Oct 06.
Typ publikacji :
Journal Article
MeSH Terms :
Glutathione Transferase/*metabolism
Receptors, Aryl Hydrocarbon/*metabolism
Base Sequence ; Electrophoretic Mobility Shift Assay ; Enzyme Inhibitors/pharmacology ; Glutathione Transferase/genetics ; Hep G2 Cells ; Humans ; Promoter Regions, Genetic ; Receptors, Aryl Hydrocarbon/agonists ; Transcription Initiation Site ; Transcriptional Activation/drug effects ; beta-Naphthoflavone/pharmacology
Czasopismo naukowe
Tytuł :
Development of a competitive binding homogeneous mobility shift assay for the quantification of adalimumab levels in patient serum.
Autorzy :
Hock BD; Haematology Research Group, Christchurch Hospital, Christchurch, New Zealand. Electronic address: .
Smith SM; Immunology Section, Canterbury Health Laboratories, Christchurch, New Zealand.
McEntyre CJ; Specialist Chemistry Section, Canterbury Health Laboratories, Christchurch, New Zealand.
McKenzie JL; Haematology Research Group, Christchurch Hospital, Christchurch, New Zealand.
Sies C; Specialist Chemistry Section, Canterbury Health Laboratories, Christchurch, New Zealand.
Keating PE; Immunology Section, Canterbury Health Laboratories, Christchurch, New Zealand.
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Źródło :
Journal of immunological methods [J Immunol Methods] 2019 Nov; Vol. 474, pp. 112672. Date of Electronic Publication: 2019 Sep 13.
Typ publikacji :
Comparative Study; Journal Article; Validation Study
MeSH Terms :
Electrophoretic Mobility Shift Assay*
Adalimumab/*blood
Drug Monitoring/*methods
Tumor Necrosis Factor Inhibitors/*blood
Enzyme-Linked Immunosorbent Assay ; Humans ; Limit of Detection ; Predictive Value of Tests ; Reproducibility of Results
Czasopismo naukowe
Tytuł :
SpoVG Modulates Cell Aggregation in Staphylococcus aureus by Regulating sasC Expression and Extracellular DNA Release.
Autorzy :
Zhu Q; Department of Oncology, The First Affiliated Hospital, University of Science and Technology of China, Hefei, Anhui, China.
Liu B; Department of Oncology, The First Affiliated Hospital, University of Science and Technology of China, Hefei, Anhui, China.
Sun B; Department of Oncology, The First Affiliated Hospital, University of Science and Technology of China, Hefei, Anhui, China .
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Źródło :
Applied and environmental microbiology [Appl Environ Microbiol] 2020 Jul 20; Vol. 86 (15). Date of Electronic Publication: 2020 Jul 20 (Print Publication: 2020).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Gene Expression Regulation, Bacterial*
Microbial Interactions*
Bacterial Proteins/*physiology
DNA, Bacterial/*physiology
DNA-Binding Proteins/*physiology
Bacterial Proteins/genetics ; Biofilms ; DNA-Binding Proteins/genetics ; Electrophoretic Mobility Shift Assay ; Staphylococcus aureus/genetics ; Staphylococcus aureus/physiology
Czasopismo naukowe
Tytuł :
NF90 modulates processing of a subset of human pri-miRNAs.
Autorzy :
Grasso G; UMR9002 CNRS-UM, Institut de Génétique Humaine-Université de Montpellier, Gene Regulation lab, Montpellier 34396, France.
Higuchi T; Laboratory of Molecular Biology, Science Research Centre, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
Mac V; UMR9002 CNRS-UM, Institut de Génétique Humaine-Université de Montpellier, Gene Regulation lab, Montpellier 34396, France.
Barbier J; UMR9002 CNRS-UM, Institut de Génétique Humaine-Université de Montpellier, Gene Regulation lab, Montpellier 34396, France.
Helsmoortel M; UMR9002 CNRS-UM, Institut de Génétique Humaine-Université de Montpellier, Gene Regulation lab, Montpellier 34396, France.
Lorenzi C; UMR9002 CNRS-UM, Institut de Génétique Humaine-Université de Montpellier, Artificial Intelligence and Gene Regulation lab, Montpellier 34396, France.
Sanchez G; UMR9002 CNRS-UM, Institut de Génétique Humaine-Université de Montpellier, Gene Regulation lab, Montpellier 34396, France.
Bello M; UMR9002 CNRS-UM, Institut de Génétique Humaine-Université de Montpellier, Gene Regulation lab, Montpellier 34396, France.
Ritchie W; UMR9002 CNRS-UM, Institut de Génétique Humaine-Université de Montpellier, Artificial Intelligence and Gene Regulation lab, Montpellier 34396, France.
Sakamoto S; Laboratory of Molecular Biology, Science Research Centre, Kochi Medical School, Kochi University, Kochi 783-8505, Japan.
Kiernan R; UMR9002 CNRS-UM, Institut de Génétique Humaine-Université de Montpellier, Gene Regulation lab, Montpellier 34396, France.
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Źródło :
Nucleic acids research [Nucleic Acids Res] 2020 Jul 09; Vol. 48 (12), pp. 6874-6888.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Inverted Repeat Sequences/*genetics
MicroRNAs/*genetics
Neoplasms/*genetics
Nuclear Factor 90 Proteins/*genetics
Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Neoplastic/genetics ; Genome, Human/genetics ; Humans ; MicroRNAs/biosynthesis ; Nuclear Factor 90 Proteins/antagonists & inhibitors ; RNA Processing, Post-Transcriptional/genetics
Czasopismo naukowe
Tytuł :
Detection of RNA-protein interactions using a highly sensitive non-radioactive electrophoretic mobility shift assay.
Autorzy :
Daras G; Department of Biotechnology, Agricultural University of Athens, Athens, Greece.
Alatzas A; Department of Biotechnology, Agricultural University of Athens, Athens, Greece.
Tsitsekian D; Department of Biotechnology, Agricultural University of Athens, Athens, Greece.
Templalexis D; Department of Biotechnology, Agricultural University of Athens, Athens, Greece.
Rigas S; Department of Biotechnology, Agricultural University of Athens, Athens, Greece.
Hatzopoulos P; Department of Biotechnology, Agricultural University of Athens, Athens, Greece.
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Źródło :
Electrophoresis [Electrophoresis] 2019 May; Vol. 40 (9), pp. 1365-1371. Date of Electronic Publication: 2019 Feb 05.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
DNA-Binding Proteins/*analysis
Electrophoretic Mobility Shift Assay/*methods
RNA-Binding Proteins/*analysis
Bacterial Proteins/metabolism ; Biotinylation ; Electrophoretic Mobility Shift Assay/standards ; Horseradish Peroxidase/metabolism ; Nucleic Acids/metabolism ; Protein Binding ; Sequence Analysis, RNA
Czasopismo naukowe
Tytuł :
Analysis of Transcriptional Regulation in Bone Cells.
Autorzy :
Jin H; South West Thames Regional Genetics Laboratory, St George's Hospital, London, UK. .; Kennedy Institute of Rheumatology, London, UK. .
Sophocleous A; Department of Life Sciences, School of Sciences, European University Cyprus, Nicosia, Cyprus.
Azfer A; Rheumatic Diseases Unit, Centre for Genomics and Experimental Medicine, MRC IGMM, Western General Hospital, University of Edinburgh, Edinburgh, UK.
Ralston SH; Rheumatic Diseases Unit, Centre for Genomics and Experimental Medicine, MRC IGMM, Western General Hospital, University of Edinburgh, Edinburgh, UK.
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1914, pp. 145-167.
Typ publikacji :
Journal Article
MeSH Terms :
Gene Expression Regulation*
Transcription, Genetic*
Bone and Bones/*cytology
Chromatin Immunoprecipitation/*methods
Electrophoretic Mobility Shift Assay/*methods
CRISPR-Cas Systems/genetics ; Cell Culture Techniques/instrumentation ; Cell Culture Techniques/methods ; Cell Line, Tumor ; Cell Separation/instrumentation ; Cell Separation/methods ; Chromatin Immunoprecipitation/instrumentation ; Electrophoretic Mobility Shift Assay/instrumentation ; Flow Cytometry/instrumentation ; Flow Cytometry/methods ; Gene Editing/instrumentation ; Gene Editing/methods ; Genes, Reporter/genetics ; Humans ; Promoter Regions, Genetic/genetics
Czasopismo naukowe
Tytuł :
XPO5 promotes primary miRNA processing independently of RanGTP.
Autorzy :
Wang J; Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO, USA.
Lee JE; Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO, USA.; ArcherDX, Boulder, CO, USA.
Riemondy K; Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO, USA.; RNA Bioscience Initiative, University of Colorado School of Medicine, Aurora, CO, USA.
Yu Y; Department of Developmental Biology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 252, New York, NY, USA.
Marquez SM; Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO, USA.
Lai EC; Department of Developmental Biology, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, Box 252, New York, NY, USA.
Yi R; Department of Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO, USA. .
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Źródło :
Nature communications [Nat Commun] 2020 Apr 15; Vol. 11 (1), pp. 1845. Date of Electronic Publication: 2020 Apr 15.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms :
Guanosine Triphosphate/*metabolism
Karyopherins/*metabolism
MicroRNAs/*metabolism
Animals ; Electrophoretic Mobility Shift Assay ; HEK293 Cells ; Humans ; Karyopherins/genetics ; Mice ; Plasmids/genetics ; Polymerase Chain Reaction ; RNA, Messenger/metabolism
Czasopismo naukowe
Tytuł :
Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins.
Autorzy :
Speca DJ; Department of Pharmacology, UC Davis School of Medicine.
Diaz E; Department of Pharmacology, UC Davis School of Medicine; .
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Źródło :
Journal of visualized experiments : JoVE [J Vis Exp] 2020 Mar 29 (157). Date of Electronic Publication: 2020 Mar 29.
Typ publikacji :
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media
MeSH Terms :
Brain/*metabolism
Electrophoretic Mobility Shift Assay/*methods
Membrane Proteins/*metabolism
Animals ; Lipoylation/physiology ; Mice
Czasopismo naukowe
Tytuł :
The crystal structure of human forkhead box N1 in complex with DNA reveals the structural basis for forkhead box family specificity.
Autorzy :
Newman JA; Structural Genomics Consortium, University of Oxford, Oxford OX3 7DQ, United Kingdom.
Aitkenhead H; Structural Genomics Consortium, University of Oxford, Oxford OX3 7DQ, United Kingdom.
Gavard AE; Structural Genomics Consortium, University of Oxford, Oxford OX3 7DQ, United Kingdom.
Rota IA; Department of Paediatrics and the Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom.
Handel AE; Department of Paediatrics and the Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom.; Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford OX3 9DU, United Kingdom.
Hollander GA; Department of Paediatrics and the Weatherall Institute of Molecular Medicine, University of Oxford, Oxford OX3 9DS, United Kingdom.; Paediatric Immunology, Department of Biomedicine, University of Basel and University Children's Hospital Basel, 4056 Basel, Switzerland.
Gileadi O; Structural Genomics Consortium, University of Oxford, Oxford OX3 7DQ, United Kingdom .
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Źródło :
The Journal of biological chemistry [J Biol Chem] 2020 Mar 06; Vol. 295 (10), pp. 2948-2958. Date of Electronic Publication: 2019 Dec 30.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
DNA/*metabolism
Forkhead Transcription Factors/*metabolism
Amino Acid Sequence ; Base Sequence ; Binding Sites ; Crystallography, X-Ray ; DNA/chemistry ; DNA Methylation ; Electrophoretic Mobility Shift Assay ; Forkhead Transcription Factors/chemistry ; Forkhead Transcription Factors/genetics ; Humans ; Protein Binding ; Protein Conformation, alpha-Helical ; Protein Structure, Tertiary ; Recombinant Proteins/biosynthesis ; Recombinant Proteins/chemistry ; Recombinant Proteins/isolation & purification ; Sequence Alignment
Czasopismo naukowe
Tytuł :
Silver Ions Caused Faster Diffusive Dynamics of Histone-Like Nucleoid-Structuring Proteins in Live Bacteria.
Autorzy :
Sadoon AA; Department of Physics, University of Arkansas, Fayetteville, Arkansas, USA.; Microelectronics-Photonics Graduate Program, University of Arkansas, Fayetteville, Arkansas, USA.; Department of Physics, University of Thi Qar, Thi Qar, Iraq.
Khadka P; Department of Physics, University of Arkansas, Fayetteville, Arkansas, USA.
Freeland J; Department of Physics, University of Arkansas, Fayetteville, Arkansas, USA.; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas, USA.
Gundampati RK; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas, USA.
Manso RH; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas, USA.
Ruiz M; Department of Physics, University of Arkansas, Fayetteville, Arkansas, USA.; Department of Biological Sciences, University of Arkansas, Fayetteville, Arkansas, USA.
Krishnamurthi VR; Department of Physics, University of Arkansas, Fayetteville, Arkansas, USA.
Thallapuranam SK; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas, USA.
Chen J; Microelectronics-Photonics Graduate Program, University of Arkansas, Fayetteville, Arkansas, USA .; Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas, USA.
Wang Y; Department of Physics, University of Arkansas, Fayetteville, Arkansas, USA .; Microelectronics-Photonics Graduate Program, University of Arkansas, Fayetteville, Arkansas, USA.; Cell and Molecular Biology Program, University of Arkansas, Fayetteville, Arkansas, USA.
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Źródło :
Applied and environmental microbiology [Appl Environ Microbiol] 2020 Mar 02; Vol. 86 (6). Date of Electronic Publication: 2020 Mar 02 (Print Publication: 2020).
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Escherichia coli/*metabolism
Escherichia coli Proteins/*genetics
Histones/*metabolism
Silver/*chemistry
Electrophoretic Mobility Shift Assay ; Escherichia coli Proteins/metabolism ; Ions/chemistry
Czasopismo naukowe
Tytuł :
Ultraviolet B-induced MdWRKY72 expression promotes anthocyanin synthesis in apple.
Autorzy :
Hu J; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Fang H; College of Forestry, Shandong Agricultural University, Tai'an, Shandong, China; State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River, Tai'an, Shandong, China.
Wang J; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Yue X; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Su M; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Mao Z; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Zou Q; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Jiang H; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Guo Z; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Yu L; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Feng T; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Lu L; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Peng Z; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Zhang Z; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China.
Wang N; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China. Electronic address: .
Chen X; State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong, China; College of Horticulture Sciences, Shandong Agricultural University, Tai'an, Shandong, China. Electronic address: .
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Źródło :
Plant science : an international journal of experimental plant biology 2020 Mar; Vol. 292, pp. 110377. Date of Electronic Publication: 2020 Jan 13.
Typ publikacji :
Journal Article
MeSH Terms :
Anthocyanins/*biosynthesis
Malus/*genetics
Plant Proteins/*genetics
Transcription Factors/*genetics
Amino Acid Sequence ; Anthocyanins/genetics ; Base Sequence ; Chromatin Immunoprecipitation ; Electrophoretic Mobility Shift Assay ; Malus/metabolism ; Phylogeny ; Plant Proteins/chemistry ; Plant Proteins/metabolism ; Plants, Genetically Modified/genetics ; Plants, Genetically Modified/metabolism ; Sequence Alignment ; Transcription Factors/chemistry ; Transcription Factors/metabolism
Czasopismo naukowe
Tytuł :
Interaction between thrombin and oligonucleotide RA36 is a two-stage process.
Autorzy :
Antipova OM; Apto-Pharm Ltd, Kolomensky dr., 13A, 115564, Moscow, Russia; Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1-40, 119991, Moscow, Russia. Electronic address: .
Solius GM; Apto-Pharm Ltd, Kolomensky dr., 13A, 115564, Moscow, Russia; Institute of Gene Biology, Russian Academy of Sciences, Vavilova str., 34/5, 119334, Moscow, Russia.
Panteleev DY; Institute of Gene Biology, Russian Academy of Sciences, Vavilova str., 34/5, 119334, Moscow, Russia.
Reshetnikov RV; Apto-Pharm Ltd, Kolomensky dr., 13A, 115564, Moscow, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Trubetskaya str. 8-2, 119048, Moscow, Russia.
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Źródło :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Feb 19; Vol. 522 (4), pp. 1037-1040. Date of Electronic Publication: 2019 Dec 06.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Oligonucleotides/*metabolism
Thrombin/*metabolism
Electrophoretic Mobility Shift Assay ; Humans ; Protein Binding
Czasopismo naukowe
Tytuł :
Dissecting the Recombination Mediator Activity of BRCA2 Using Biochemical Methods.
Autorzy :
von Nicolai C; Institut Curie, PSL Research University, CNRS, UMR3348, Orsay, France; Paris Sud University, Paris-Saclay University, CNRS, UMR3348, Orsay, France.
Ehlén Å; Institut Curie, PSL Research University, CNRS, UMR3348, Orsay, France; Paris Sud University, Paris-Saclay University, CNRS, UMR3348, Orsay, France.
Martinez JS; Institut Curie, PSL Research University, CNRS, UMR3348, Orsay, France; Paris Sud University, Paris-Saclay University, CNRS, UMR3348, Orsay, France.
Carreira A; Institut Curie, PSL Research University, CNRS, UMR3348, Orsay, France; Paris Sud University, Paris-Saclay University, CNRS, UMR3348, Orsay, France. Electronic address: .
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Źródło :
Methods in enzymology [Methods Enzymol] 2018; Vol. 600, pp. 479-511.
Typ publikacji :
Journal Article
MeSH Terms :
Recombinational DNA Repair*
BRCA2 Protein/*metabolism
DNA, Single-Stranded/*metabolism
Electrophoretic Mobility Shift Assay/*methods
BRCA2 Protein/chemistry ; BRCA2 Protein/isolation & purification ; Cell Cycle Proteins/chemistry ; Cell Cycle Proteins/isolation & purification ; Cell Cycle Proteins/metabolism ; DNA Breaks, Double-Stranded ; DNA, Single-Stranded/chemistry ; DNA-Binding Proteins/chemistry ; DNA-Binding Proteins/isolation & purification ; DNA-Binding Proteins/metabolism ; Electrophoresis, Agar Gel/methods ; Electrophoresis, Polyacrylamide Gel/instrumentation ; Electrophoresis, Polyacrylamide Gel/methods ; Electrophoretic Mobility Shift Assay/instrumentation ; Rad51 Recombinase/chemistry ; Rad51 Recombinase/isolation & purification ; Rad51 Recombinase/metabolism ; Staining and Labeling/instrumentation ; Staining and Labeling/methods ; Substrate Specificity
Czasopismo naukowe
Tytuł :
PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida.
Autorzy :
Yin K; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Peng Y; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Ahmed MAH; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Ma J; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China.
Xu R; Yantai Tianyuan Aquatic Co. Ltd., Yantai, Shandong, China.
Zhang Y; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai, 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, 200237, China.
Ma Y; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai, 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, 200237, China. Electronic address: .
Wang Q; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China; Shanghai Collaborative Innovation Center for Biomanufacturing Technology, 130 Meilong Road, Shanghai, 200237, China; Shanghai Engineering Research Center of Maricultured Animal Vaccines, Shanghai, 200237, China. Electronic address: .
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Źródło :
Microbiological research [Microbiol Res] 2020 Feb; Vol. 232, pp. 126349. Date of Electronic Publication: 2019 Oct 09.
Typ publikacji :
Journal Article
MeSH Terms :
Bacterial Proteins/*metabolism
Edwardsiella/*metabolism
Enterobacteriaceae Infections/*veterinary
Virulence Factors/*genetics
Animals ; Bacterial Proteins/genetics ; Edwardsiella/genetics ; Electrophoretic Mobility Shift Assay/veterinary ; Enterobacteriaceae Infections/microbiology ; Fish Diseases/microbiology ; Gene Expression Regulation, Bacterial ; Leucyl Aminopeptidase/genetics ; Leucyl Aminopeptidase/metabolism ; Promoter Regions, Genetic ; Reactive Oxygen Species ; Type III Secretion Systems/metabolism ; Type VI Secretion Systems/metabolism ; Virulence/genetics ; Virulence Factors/metabolism
SCR Organism :
Edwardsiella piscicida
Czasopismo naukowe
Tytuł :
Identification of genes regulated by the two-component system response regulator NarP of Actinobacillus pleuropneumoniae via DNA-affinity-purified sequencing.
Autorzy :
Zhang Q; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
Huang Q; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
Fang Q; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
Li H; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
Tang H; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
Zou G; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
Wang D; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
Li S; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China.
Bei W; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Wuhan, Hubei, 430070, China; International Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, Hubei, 430070, China.
Chen H; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Wuhan, Hubei, 430070, China; International Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, Hubei, 430070, China.
Li L; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Wuhan, Hubei, 430070, China; International Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, Hubei, 430070, China. Electronic address: .
Zhou R; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, Hubei, 430070, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Wuhan, Hubei, 430070, China; International Research Center for Animal Disease, Ministry of Science and Technology of the People's Republic of China, Wuhan, Hubei, 430070, China. Electronic address: .
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Źródło :
Microbiological research [Microbiol Res] 2020 Jan; Vol. 230, pp. 126343. Date of Electronic Publication: 2019 Sep 10.
Typ publikacji :
Evaluation Study; Journal Article
MeSH Terms :
Actinobacillus pleuropneumoniae/*genetics
Bacterial Proteins/*metabolism
DNA-Binding Proteins/*metabolism
Sequence Analysis, DNA/*methods
Actinobacillus pleuropneumoniae/metabolism ; Bacterial Proteins/genetics ; DNA-Binding Proteins/genetics ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Bacterial ; Nitrates/metabolism ; Nitrites/metabolism ; Promoter Regions, Genetic ; Protein Binding ; Protein Kinases/genetics ; Protein Kinases/metabolism
Czasopismo naukowe
Tytuł :
Three transcriptional regulators positively regulate the biosynthesis of polycyclic tetramate macrolactams in Streptomyces xiamenensis 318.
Autorzy :
Bu XL; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Weng JY; Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
He-Lin Yu; Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Xu MJ; Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China.
Xu J; State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People's Republic of China. .
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Źródło :
Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2020 Jan; Vol. 104 (2), pp. 701-711. Date of Electronic Publication: 2019 Dec 09.
Typ publikacji :
Journal Article
MeSH Terms :
Gene Expression Regulation, Bacterial*
Biological Products/*metabolism
Polycyclic Compounds/*metabolism
Streptomyces/*genetics
Streptomyces/*metabolism
Transcription Factors/*metabolism
Biosynthetic Pathways/genetics ; DNA, Bacterial/metabolism ; Electrophoretic Mobility Shift Assay ; Heat-Shock Response ; Protein Binding ; Streptomyces/radiation effects ; Temperature ; Transcription Factors/genetics ; Transcription, Genetic/radiation effects
SCR Organism :
Streptomyces xiamenensis
Czasopismo naukowe
Tytuł :
Green-Synthesized FeSO4 Nanoparticles Exhibit Antibacterial and Cytotoxic Activity by DNA Degradation.
Autorzy :
Bharath B; Department of Biotechnology, National Institute of Technology, Warangal, Telangana-506004, India.
Sasidharan S; Department of Biotechnology, National Institute of Technology, Warangal, Telangana-506004, India.
Bhamidipati SK; Department of Biotechnology, National Institute of Technology, Warangal, Telangana-506004, India.
Saudagar P; Department of Biotechnology, National Institute of Technology, Warangal, Telangana-506004, India.
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Źródło :
Current pharmaceutical biotechnology [Curr Pharm Biotechnol] 2020; Vol. 21 (7), pp. 587-595.
Typ publikacji :
Journal Article
MeSH Terms :
DNA Damage*
Anti-Bacterial Agents/*chemistry
Antineoplastic Agents/*chemistry
Ferrous Compounds/*chemistry
Green Chemistry Technology/*methods
Nanoparticles/*chemistry
Animals ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/toxicity ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/toxicity ; Artemia/drug effects ; Artemia/genetics ; Biofilms/drug effects ; Biofilms/growth & development ; Electrophoretic Mobility Shift Assay ; Ferrous Compounds/pharmacology ; Ferrous Compounds/toxicity ; Flowers/chemistry ; Gram-Negative Bacteria/drug effects ; Gram-Positive Bacteria/drug effects ; Hibiscus ; Microbial Sensitivity Tests ; Plant Extracts/chemistry ; Spectroscopy, Fourier Transform Infrared ; Survival Analysis
Czasopismo naukowe
Tytuł :
Fidelity of prespacer capture and processing is governed by the PAM-mediated interactions of Cas1-2 adaptation complex in CRISPR-Cas type I-E system.
Autorzy :
Yoganand KN; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India.
Muralidharan M; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India.
Nimkar S; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India.
Anand B; Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, India .
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Źródło :
The Journal of biological chemistry [J Biol Chem] 2019 Dec 27; Vol. 294 (52), pp. 20039-20053. Date of Electronic Publication: 2019 Nov 20.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
CRISPR-Associated Proteins/*metabolism
CRISPR-Cas Systems/*genetics
Escherichia coli/*metabolism
Binding Sites ; CRISPR-Associated Proteins/chemistry ; CRISPR-Associated Proteins/genetics ; DNA/chemistry ; DNA/metabolism ; Electrophoretic Mobility Shift Assay ; Endodeoxyribonucleases/chemistry ; Endodeoxyribonucleases/genetics ; Endodeoxyribonucleases/metabolism ; Endonucleases/chemistry ; Endonucleases/genetics ; Endonucleases/metabolism ; Escherichia coli Proteins/chemistry ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Exonucleases/metabolism ; Protein Binding ; Protein Structure, Quaternary
Czasopismo naukowe
Tytuł :
X-ray crystal structure of putative transcription regulator lmo2088 from Listeria monocytogenes.
Autorzy :
Samad A; Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China; Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Li Y; Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Zhang C; Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Chen F; Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Zeng W; Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Fan X; Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Jin T; Department of Obstetrics and Gynecology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China; Division of Molecular Medicine, Hefei National Laboratory for Physical Sciences at Microscale, CAS Key Laboratory of Innate Immunity and Chronic Disease, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China; CAS Center for Excellence in Molecular Cell Science, Shanghai, China. Electronic address: .
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Źródło :
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2019 Dec 03; Vol. 520 (2), pp. 434-440. Date of Electronic Publication: 2019 Oct 10.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Bacterial Proteins/*chemistry
Bacterial Proteins/*metabolism
Listeria monocytogenes/*chemistry
Bacterial Proteins/genetics ; Binding Sites ; Calorimetry ; Crystallography, X-Ray ; DNA/chemistry ; DNA/metabolism ; Electrophoretic Mobility Shift Assay ; Fluorescence Polarization ; Listeria monocytogenes/genetics ; Models, Molecular ; Protein Conformation ; Transcription Factors/chemistry ; Transcription Factors/genetics ; Transcription Factors/metabolism
Czasopismo naukowe

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