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Title:
Temperature dependent in vitro binding and release of target DNA by Cas9 enzyme.
Authors:
David SR; Raman Research Institute, Bangalore, Karnataka, 560080, India.
Maheshwaram SK; Raman Research Institute, Bangalore, Karnataka, 560080, India.
Shet D; Raman Research Institute, Bangalore, Karnataka, 560080, India.
Lakshminarayana MB; Raman Research Institute, Bangalore, Karnataka, 560080, India.
Soni GV; Raman Research Institute, Bangalore, Karnataka, 560080, India. .
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Source:
Scientific reports [Sci Rep] 2022 Sep 09; Vol. 12 (1), pp. 15243. Date of Electronic Publication: 2022 Sep 09.
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
CRISPR-Associated Protein 9*
DNA*
Electrophoretic Mobility Shift Assay ; RNA, Guide, CRISPR-Cas Systems/genetics ; Temperature
Academic Journal
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Title:
Discovering the DNA-Binding Consensus of the Thermus thermophilus HB8 Transcriptional Regulator TTHA1359.
Authors:
Teague JL; Department of Chemistry and Biochemistry, Kennesaw State University, Kennesaw, GA 30144, USA.
Barrows JK; Department of Chemistry and Biochemistry, Kennesaw State University, Kennesaw, GA 30144, USA.
Baafi CA; Department of Chemistry and Biochemistry, Kennesaw State University, Kennesaw, GA 30144, USA.
Van Dyke MW; Department of Chemistry and Biochemistry, Kennesaw State University, Kennesaw, GA 30144, USA.
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Source:
International journal of molecular sciences [Int J Mol Sci] 2021 Sep 17; Vol. 22 (18). Date of Electronic Publication: 2021 Sep 17.
Publication Type:
Journal Article
MeSH Terms:
Thermus thermophilus/*metabolism
Transcription Factors/*metabolism
Computational Biology ; Electrophoretic Mobility Shift Assay ; Extremophiles/metabolism ; Gene Expression Regulation, Bacterial/genetics ; Gene Expression Regulation, Bacterial/physiology ; Thermus thermophilus/genetics ; Transcription Factors/genetics
Academic Journal
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Title:
A possible role of gas-phase electrophoretic mobility molecular analysis (nES GEMMA) in extracellular vesicle research.
Authors:
Steinberger S; Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164 CTA, A-1060, Vienna, Austria.
Karuthedom George S; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Lauková L; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Weiss R; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Tripisciano C; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Birner-Gruenberger R; Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164 CTA, A-1060, Vienna, Austria.
Weber V; Center for Biomedical Technology, Department for Biomedical Research, Danube University Krems, Krems, Austria.
Allmaier G; Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164 CTA, A-1060, Vienna, Austria.
Weiss VU; Institute of Chemical Technologies and Analytics, TU Wien, Getreidemarkt 9/164 CTA, A-1060, Vienna, Austria. .
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Source:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2021 Dec; Vol. 413 (30), pp. 7341-7352. Date of Electronic Publication: 2021 Oct 07.
Publication Type:
Journal Article
MeSH Terms:
Electrophoretic Mobility Shift Assay/*methods
Extracellular Vesicles/*metabolism
Spectrometry, Mass, Electrospray Ionization/*methods
Gases ; Humans ; Spectrometry, Mass, Electrospray Ionization/instrumentation
Academic Journal
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Title:
GntR-like SCO3932 Protein Provides a Link between Actinomycete Integrative and Conjugative Elements and Secondary Metabolism.
Authors:
Pawlik KJ; Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Zelkowski M; Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Biernacki M; Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Litwinska K; Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Jaworski P; Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
Kotowska M; Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
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Source:
International journal of molecular sciences [Int J Mol Sci] 2021 Nov 01; Vol. 22 (21). Date of Electronic Publication: 2021 Nov 01.
Publication Type:
Journal Article
MeSH Terms:
Biosynthetic Pathways*
Actinobacteria/*genetics
Streptomyces/*growth & development
Transcription Factors/*genetics
Bacterial Proteins/genetics ; Chromatin Immunoprecipitation ; Chromatography, Affinity ; Cloning, Molecular ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Bacterial ; Promoter Regions, Genetic ; Secondary Metabolism ; Streptomyces/genetics
SCR Organism:
Streptomyces ambofaciens
Academic Journal
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Title:
Transcription factors Krüppel-like factor 4 and paired box 5 regulate the expression of the Grainyhead-like genes.
Authors:
Kotarba G; Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland.
Taracha-Wisniewska A; Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland.
Miller M; Laboratory of Bioinformatics, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, Poland.
Dabrowski M; Laboratory of Bioinformatics, Nencki Institute of Experimental Biology of Polish Academy of Sciences, Warsaw, Poland.
Wilanowski T; Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland.
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Source:
PloS one [PLoS One] 2021 Sep 27; Vol. 16 (9), pp. e0257977. Date of Electronic Publication: 2021 Sep 27 (Print Publication: 2021).
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Gene Expression Regulation*
Kruppel-Like Transcription Factors/*physiology
PAX5 Transcription Factor/*physiology
Transcription Factors/*metabolism
Animals ; Chromatin Immunoprecipitation ; Computer Simulation ; DNA-Binding Proteins/metabolism ; Electrophoretic Mobility Shift Assay ; Gene Frequency ; HEK293 Cells ; Humans ; Kruppel-Like Factor 4 ; Kruppel-Like Transcription Factors/genetics ; Mice ; PAX5 Transcription Factor/metabolism ; Polymorphism, Single Nucleotide/genetics ; Real-Time Polymerase Chain Reaction ; Repressor Proteins/metabolism ; Transcription Factors/genetics
Academic Journal
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Title:
Differential expression and transcription factor binding associated with genotype at a pharmacogenetic variant in OPRD1 .
Authors:
Crist RC; Department of Psychiatry, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Arauco-Shapiro G; Department of Psychiatry, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Zhang A; Department of Psychiatry, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Reiner BC; Department of Psychiatry, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Berrettini WH; Department of Psychiatry, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.; Geisinger Clinic, Danville, PA, USA.
Doyle GA; Department of Psychiatry, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
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Source:
The American journal of drug and alcohol abuse [Am J Drug Alcohol Abuse] 2021 Sep 03; Vol. 47 (5), pp. 581-589. Date of Electronic Publication: 2021 Aug 18.
Publication Type:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
MeSH Terms:
Pharmacogenomic Variants*
Heterogeneous Nuclear Ribonucleoprotein D0/*metabolism
Ku Autoantigen/*metabolism
Receptors, Opioid, delta/*genetics
Transcription Factors/*metabolism
Alleles ; Electrophoretic Mobility Shift Assay ; Genotype ; Humans ; Luciferases, Firefly ; Protein Binding/genetics ; Quantitative Trait Loci/genetics
Academic Journal
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Title:
The genetic consequences of dog breed formation-Accumulation of deleterious genetic variation and fixation of mutations associated with myxomatous mitral valve disease in cavalier King Charles spaniels.
Authors:
Axelsson E; Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Ljungvall I; Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Bhoumik P; Translational Medicine, Novartis Institutes for BioMedical Research, Basel, Switzerland.
Conn LB; Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Muren E; Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Ohlsson Å; Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Olsen LH; Department of Veterinary and Animal Sciences, University of Copenhagen, Copenhagen, Denmark.
Engdahl K; Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Hagman R; Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Hanson J; Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Kryvokhyzha D; Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Pettersson M; Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.
Grenet O; Translational Medicine, Novartis Institutes for BioMedical Research, Basel, Switzerland.
Moggs J; Translational Medicine, Novartis Institutes for BioMedical Research, Basel, Switzerland.
Del Rio-Espinola A; Translational Medicine, Novartis Institutes for BioMedical Research, Basel, Switzerland.
Epe C; Elanco Animal Health, Greenfield, Indiana, United States of America.
Taillon B; Elanco Animal Health, Greenfield, Indiana, United States of America.
Tawari N; Elanco Animal Health, Greenfield, Indiana, United States of America.
Mane S; Elanco Animal Health, Greenfield, Indiana, United States of America.
Hawkins T; Elanco Animal Health, Greenfield, Indiana, United States of America.
Hedhammar Å; Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Gruet P; Novartis Animal Health, St: Aubin, Switzerland.
Häggström J; Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Lindblad-Toh K; Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts, United States of America.
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Source:
PLoS genetics [PLoS Genet] 2021 Sep 02; Vol. 17 (9), pp. e1009726. Date of Electronic Publication: 2021 Sep 02 (Print Publication: 2021).
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Genetic Variation*
Mutation*
Dog Diseases/*genetics
Dogs/*genetics
Heart Valve Diseases/*veterinary
Mitral Valve/*pathology
Alleles ; Animals ; Electrophoretic Mobility Shift Assay ; Gene Expression ; Heart Valve Diseases/genetics
Academic Journal
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Title:
Characterization of Interaction of the MBP-Tagged MuRif1-C-Terminal Domain with G-Quadruplex DNA by SPR.
Authors:
Ghadiri H; Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. .
Alavi S; Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. .
Dabirmanesh B; Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. .
Khajeh K; Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. .; Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
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Source:
Biochemistry. Biokhimiia [Biochemistry (Mosc)] 2021 Jul; Vol. 86 (7), pp. 898-905.
Publication Type:
Journal Article
MeSH Terms:
G-Quadruplexes*
DNA/*metabolism
Telomere-Binding Proteins/*metabolism
Animals ; Electrophoretic Mobility Shift Assay ; Kinetics ; Mice ; Protein Binding ; Surface Plasmon Resonance
Academic Journal
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Title:
Determination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis.
Authors:
Pan Y; University of California, Berkeley - UCSF Graduate Program in Bioengineering, Berkeley, CA, 94720, USA.
Sackmann EK; Labcyte Inc., 1190 Borregas Ave, Sunnyvale, CA, 94089, USA.
Wypisniak K; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, 94158 USA.
Hornsby M; Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, 94158 USA.
Datwani SS; Labcyte Inc., 1190 Borregas Ave, Sunnyvale, CA, 94089, USA.
Herr AE; University of California, Berkeley - UCSF Graduate Program in Bioengineering, Berkeley, CA, 94720, USA.; Department of Bioengineering, University of California, Berkeley, CA, 94720, USA.
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Source:
Scientific reports [Sci Rep] 2016 Dec 23; Vol. 6, pp. 39774. Date of Electronic Publication: 2016 Dec 23.
Publication Type:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
MeSH Terms:
Antibody Affinity*
Electrophoresis, Polyacrylamide Gel*
Electrophoretic Mobility Shift Assay*
Immunoglobulin Fab Fragments/*chemistry
Animals ; Humans ; Recombinant Proteins/chemistry
Academic Journal
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Title:
Electromobility Shift Assay Reveals Evidence in Favor of Allele-Specific Binding of RUNX1 to the 5' Hypersensitive Site 4-Locus Control Region.
Authors:
Dehghani H; a Genetics Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran , Iran.
Ghobakhloo S; a Genetics Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran , Iran.
Neishabury M; a Genetics Research Center, University of Social Welfare and Rehabilitation Sciences , Tehran , Iran.
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Source:
Hemoglobin [Hemoglobin] 2016 Aug; Vol. 40 (4), pp. 236-9.
Publication Type:
Journal Article
MeSH Terms:
Alleles*
Electrophoretic Mobility Shift Assay*
Locus Control Region*
Core Binding Factor Alpha 2 Subunit/*metabolism
Binding Sites ; Humans ; Inverted Repeat Sequences ; Phenotype ; Protein Binding
Academic Journal
Full Text  Link opens in a new window Full TextDetails
Title:
Transcriptional Suppression of Diabetic Nephropathy with Novel Gene Silencer Pyrrole-Imidazole Polyamides Preventing USF1 Binding to the TGF-β1 Promoter.
Authors:
Okamura M; Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Ooyaguchi-kami 30-1, Itabashi-ku, Tokyo 173-8610, Japan.
Fukuda N; Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Ooyaguchi-kami 30-1, Itabashi-ku, Tokyo 173-8610, Japan.; Division of Cell Regeneration and Transplantation, Department of Functional Morphology, Nihon University School of Medicine, Tokyo 173-8610, Japan.
Horikoshi S; Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Ooyaguchi-kami 30-1, Itabashi-ku, Tokyo 173-8610, Japan.
Kobayashi H; Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Ooyaguchi-kami 30-1, Itabashi-ku, Tokyo 173-8610, Japan.
Tsunemi A; Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Ooyaguchi-kami 30-1, Itabashi-ku, Tokyo 173-8610, Japan.
Akiya Y; Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Ooyaguchi-kami 30-1, Itabashi-ku, Tokyo 173-8610, Japan.
Endo M; Faculty of Human Health Science, Hachinohe Gakuin University, Hachinohe, Aomori 031-8588, Japan.
Matsumoto T; Division of Cell Regeneration and Transplantation, Department of Functional Morphology, Nihon University School of Medicine, Tokyo 173-8610, Japan.
Abe M; Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Ooyaguchi-kami 30-1, Itabashi-ku, Tokyo 173-8610, Japan.
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Source:
International journal of molecular sciences [Int J Mol Sci] 2021 Apr 29; Vol. 22 (9). Date of Electronic Publication: 2021 Apr 29.
Publication Type:
Journal Article
MeSH Terms:
Gene Silencing*
Diabetic Nephropathies/*drug therapy
Transforming Growth Factor beta1/*antagonists & inhibitors
Upstream Stimulatory Factors/*antagonists & inhibitors
Albuminuria/etiology ; Albuminuria/prevention & control ; Animals ; Blood Urea Nitrogen ; Body Weight/drug effects ; Diabetes Mellitus, Experimental/complications ; Diabetic Nephropathies/genetics ; Diabetic Nephropathies/urine ; Drug Design ; Drug Evaluation, Preclinical ; Electrophoretic Mobility Shift Assay ; Glucose/pharmacology ; Glycated Hemoglobin/analysis ; Kidney Glomerulus/chemistry ; Kidney Tubules/chemistry ; Male ; Mesangial Cells/drug effects ; Mesangial Cells/metabolism ; Osteopontin/analysis ; Promoter Regions, Genetic ; Protein Binding/drug effects ; Rats ; Transcription, Genetic ; Transforming Growth Factor beta1/genetics ; Upstream Stimulatory Factors/metabolism
Academic Journal
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Title:
Secondary structural choice of DNA and RNA associated with CGG/CCG trinucleotide repeat expansion rationalizes the RNA misprocessing in FXTAS.
Authors:
Ajjugal Y; Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Telangana State, 502285, India.
Kolimi N; Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Telangana State, 502285, India.
Rathinavelan T; Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Telangana State, 502285, India. .
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Source:
Scientific reports [Sci Rep] 2021 Apr 14; Vol. 11 (1), pp. 8163. Date of Electronic Publication: 2021 Apr 14.
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Ataxia/*genetics
Fragile X Mental Retardation Protein/*chemistry
Fragile X Mental Retardation Protein/*genetics
Fragile X Syndrome/*genetics
RNA, Messenger/*chemistry
Tremor/*genetics
5' Untranslated Regions ; Circular Dichroism ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation ; Humans ; Models, Molecular ; Molecular Dynamics Simulation ; Nucleic Acid Conformation ; Trinucleotide Repeat Expansion
SCR Disease Name:
Fragile X Tremor Ataxia Syndrome
Academic Journal
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Title:
Two distinct conformers of PrP type 1 of sporadic Creutzfeldt-Jakob disease with codon 129VV genotype faithfully propagate in vivo.
Authors:
Cali I; Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA. .; National Prion Disease Pathology Surveillance Center, School of Medicine, Case Western Reserve University, Cleveland, OH, USA. .
Espinosa JC; Centro de Investigación en Sanidad Animal, CISA-INIA, Madrid, Spain.
Nemani SK; Centre for Prions and Protein Folding Diseases, Brain and Aging Research Building, University of Alberta, Edmonton, Canada.
Marin-Moreno A; Centro de Investigación en Sanidad Animal, CISA-INIA, Madrid, Spain.
Camacho MV; Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
Aslam R; Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
Kitamoto T; Department of Neurological Science, Tohoku University Graduate School of Medicine, Sendai, Japan.
Appleby BS; Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.; Department of Neurology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.; Department of Psychiatry, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.; National Prion Disease Pathology Surveillance Center, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
Torres JM; Centro de Investigación en Sanidad Animal, CISA-INIA, Madrid, Spain.
Gambetti P; Department of Pathology, School of Medicine, Case Western Reserve University, Cleveland, OH, USA.
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Acta neuropathologica communications [Acta Neuropathol Commun] 2021 Mar 25; Vol. 9 (1), pp. 55. Date of Electronic Publication: 2021 Mar 25.
Publication Type:
Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
MeSH Terms:
Creutzfeldt-Jakob Syndrome/*genetics
Creutzfeldt-Jakob Syndrome/*metabolism
Prion Proteins/*chemistry
Prion Proteins/*genetics
Prion Proteins/*metabolism
Animals ; Codon ; Electrophoretic Mobility Shift Assay ; Genotype ; Humans ; Mice ; Mice, Transgenic ; Protein Isoforms
SCR Disease Name:
Creutzfeldt-Jakob Disease, Sporadic
Academic Journal
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Title:
CNBP Binds and Unfolds In Vitro G-Quadruplexes Formed in the SARS-CoV-2 Positive and Negative Genome Strands.
Authors:
Bezzi G; Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda, Rosario S200EZP, Santa Fe, Argentina.
Piga EJ; Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda, Rosario S200EZP, Santa Fe, Argentina.
Binolfi A; Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda, Rosario S200EZP, Santa Fe, Argentina.; Plataforma Argentina de Biología Estructural y Metabolómica (PLABEM), Ocampo y Esmeralda, Rosario S200EZP, Santa Fe, Argentina.
Armas P; Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda, Rosario S200EZP, Santa Fe, Argentina.
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Source:
International journal of molecular sciences [Int J Mol Sci] 2021 Mar 05; Vol. 22 (5). Date of Electronic Publication: 2021 Mar 05.
Publication Type:
Journal Article
MeSH Terms:
G-Quadruplexes*
RNA-Binding Proteins/*metabolism
SARS-CoV-2/*genetics
SARS-CoV-2/*metabolism
Circular Dichroism ; Computational Biology/methods ; Databases, Genetic ; Electrophoretic Mobility Shift Assay ; Genome, Viral/physiology ; Humans ; Proton Magnetic Resonance Spectroscopy ; Virus Replication/physiology
Academic Journal
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Title:
Characterization of the G protein-coupled receptor kinase 6 promoter reveals a functional CREB binding site.
Authors:
Stegen M; Department of Anaesthesiology and Intensive Care Medicine, Essen University Hospital and University of Duisburg-Essen, Essen, Germany.
Engler A; Department of Anaesthesiology and Intensive Care Medicine, Essen University Hospital and University of Duisburg-Essen, Essen, Germany.
Ochsenfarth C; Department of Anaesthesiology, Operative Intensive Care Medicine, Pain and Palliative Medicine, Marien Hospital Herne, Ruhr-University Bochum, Bochum, Germany.
Manthey I; Institute of Pharmacogenetics, Essen University Hospital and University of Duisburg-Essen, Essen, Germany.
Peters J; Department of Anaesthesiology and Intensive Care Medicine, Essen University Hospital and University of Duisburg-Essen, Essen, Germany.
Siffert W; Institute of Pharmacogenetics, Essen University Hospital and University of Duisburg-Essen, Essen, Germany.
Frey UH; Department of Anaesthesiology and Intensive Care Medicine, Essen University Hospital and University of Duisburg-Essen, Essen, Germany.; Department of Anaesthesiology, Operative Intensive Care Medicine, Pain and Palliative Medicine, Marien Hospital Herne, Ruhr-University Bochum, Bochum, Germany.
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PloS one [PLoS One] 2021 Feb 18; Vol. 16 (2), pp. e0247087. Date of Electronic Publication: 2021 Feb 18 (Print Publication: 2021).
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cyclic AMP Response Element-Binding Protein/*metabolism
G-Protein-Coupled Receptor Kinases/*genetics
Response Elements/*genetics
Base Sequence ; Binding Sites ; Cyclic AMP Response Element-Binding Protein/chemistry ; Electrophoretic Mobility Shift Assay ; G-Protein-Coupled Receptor Kinases/chemistry ; G-Protein-Coupled Receptor Kinases/metabolism ; Humans ; Jurkat Cells ; Mutagenesis, Site-Directed ; Promoter Regions, Genetic ; Protein Binding ; Protein Kinase C/metabolism ; RNA, Messenger/metabolism
Academic Journal
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Title:
Fluorescent thermal shift-based method for detection of NF-κB binding to double-stranded DNA.
Authors:
Leitner PD; Institute of Cell Biology, Biocenter, Medical University of Innsbruck, Innrain 80-82, 6020, Innsbruck, Austria.; Austrian Drug Screening Institute, ADSI, Innsbruck, Austria.; Department of Biotechnology and Food Engineering, MCI Technik, Innsbruck, Austria.
Vietor I; Institute of Cell Biology, Biocenter, Medical University of Innsbruck, Innrain 80-82, 6020, Innsbruck, Austria.
Huber LA; Institute of Cell Biology, Biocenter, Medical University of Innsbruck, Innrain 80-82, 6020, Innsbruck, Austria.; Austrian Drug Screening Institute, ADSI, Innsbruck, Austria.
Valovka T; Institute of Cell Biology, Biocenter, Medical University of Innsbruck, Innrain 80-82, 6020, Innsbruck, Austria. .; Department of Pediatrics I, Medical University of Innsbruck, Anichstrasse 35, 6020, Innsbruck, Austria. .
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Source:
Scientific reports [Sci Rep] 2021 Jan 27; Vol. 11 (1), pp. 2331. Date of Electronic Publication: 2021 Jan 27.
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Escherichia coli/*metabolism
NF-kappa B/*metabolism
Chromatography, Affinity ; Computational Biology ; DNA/metabolism ; Drug Discovery ; Electrophoretic Mobility Shift Assay
Academic Journal
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Title:
Antibiotic-induced DNA damage results in a controlled loss of pH homeostasis and genome instability.
Authors:
Booth JA; Department of Microbiology, University of Oslo and Oslo University Hospital, Rikshospitalet, Oslo, Norway. .
Špírek M; Department of Biology, Masaryk University, Kamenice 5/A7, 625 00, Brno, Czech Republic.; International Clinical Research Center, Center for Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Pekarska 53, 656 91, Brno, Czech Republic.
Lobie TA; Department of Microbiology, University of Oslo and Oslo University Hospital, Rikshospitalet, Oslo, Norway.
Skarstad K; Department of Microbiology, University of Oslo and Oslo University Hospital, Rikshospitalet, Oslo, Norway.
Krejci L; Department of Biology, Masaryk University, Kamenice 5/A7, 625 00, Brno, Czech Republic.; National Centre for Biomolecular Research, Masaryk University, Kamenice 5/A4, 625 00, Brno, Czech Republic.; International Clinical Research Center, Center for Biomolecular and Cellular Engineering, St. Anne's University Hospital Brno, Pekarska 53, 656 91, Brno, Czech Republic.
Bjørås M; Department of Microbiology, University of Oslo and Oslo University Hospital, Rikshospitalet, Oslo, Norway. .; Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway. .
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Source:
Scientific reports [Sci Rep] 2020 Nov 10; Vol. 10 (1), pp. 19422. Date of Electronic Publication: 2020 Nov 10.
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Anti-Bacterial Agents/*pharmacology
DNA Damage/*drug effects
DNA Damage/*genetics
Escherichia coli/*drug effects
Escherichia coli/*genetics
Genomic Instability/*drug effects
Genomic Instability/*genetics
DNA Damage/radiation effects ; Electrophoretic Mobility Shift Assay ; Escherichia coli/radiation effects ; Flow Cytometry ; Genomic Instability/radiation effects ; Hydrogen-Ion Concentration ; Microbial Viability/drug effects ; Microbial Viability/radiation effects ; Nalidixic Acid/pharmacology ; Propidium/pharmacology ; Rifampin/pharmacology ; Ultraviolet Rays
Academic Journal
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Title:
Unannotated single nucleotide polymorphisms in the TATA box of erythropoiesis genes show in vitro positive involvements in cognitive and mental disorders.
Authors:
Ponomarenko M; Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Ave, Novosibirsk, 630090, Russia. .; Novosibirsk State University, 1 Pirogova Street, Novosibirsk, 630090, Russia. .
Sharypova E; Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Ave, Novosibirsk, 630090, Russia.
Drachkova I; Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Ave, Novosibirsk, 630090, Russia.
Chadaeva I; Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Ave, Novosibirsk, 630090, Russia.
Arkova O; Institute of Gene Biology Russian Academy of Sciences, 34/5 Vavilova Street, Moscow, 119334, Russia.
Podkolodnaya O; Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Ave, Novosibirsk, 630090, Russia.
Ponomarenko P; Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Ave, Novosibirsk, 630090, Russia.
Kolchanov N; Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Ave, Novosibirsk, 630090, Russia.
Savinkova L; Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Lavrentyev Ave, Novosibirsk, 630090, Russia.
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Source:
BMC medical genetics [BMC Med Genet] 2020 Oct 22; Vol. 21 (Suppl 1), pp. 165. Date of Electronic Publication: 2020 Oct 22.
Publication Type:
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms:
Cognitive Dysfunction/*genetics
Erythropoiesis/*genetics
Hemoglobinopathies/*genetics
Mental Disorders/*genetics
TATA Box/*genetics
Astrocytes/metabolism ; Base Sequence ; Brain/metabolism ; Computational Biology ; Computer Simulation ; Electrophoretic Mobility Shift Assay ; Humans ; Polymorphism, Single Nucleotide/genetics ; alpha-Globins/genetics ; beta-Globins/genetics
Academic Journal
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Title:
Determination of binding constants for strong complexation by affinity capillary electrophoresis: the example of complexes of ester betulin derivatives with (2-hydroxypropyl)-γ-cyclodextrin.
Authors:
Sursyakova VV; Institute of Chemistry and Chemical Technology SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Akademgorodok 50/24, Krasnoyarsk, Russia, 660036. viktoria_.
Levdansky VA; Institute of Chemistry and Chemical Technology SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Akademgorodok 50/24, Krasnoyarsk, Russia, 660036.
Rubaylo AI; Institute of Chemistry and Chemical Technology SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', Akademgorodok 50/24, Krasnoyarsk, Russia, 660036.; Siberian Federal University, Svobodny pr. 79, Krasnoyarsk, Russia, 660041.; Federal Research Center 'Krasnoyarsk Science Center SB RAS', Akademgorodok 50, Krasnoyarsk, Russia, 660036.
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Source:
Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2020 Sep; Vol. 412 (23), pp. 5615-5625. Date of Electronic Publication: 2020 Jul 02.
Publication Type:
Journal Article
MeSH Terms:
Electrophoresis, Capillary/*methods
Triterpenes/*chemistry
gamma-Cyclodextrins/*chemistry
Electrophoretic Mobility Shift Assay ; Esters ; Limit of Detection ; Triterpenes/analysis
Academic Journal
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Title:
P-element Somatic Inhibitor Protein Binding a Target Sequence in dsx Pre-mRNA Conserved in Bombyx mori and Spodoptera litura .
Authors:
Wang Y; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Beibei, Chongqing 400715, China. .
Zhao Q; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Beibei, Chongqing 400715, China. .
Wan QX; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Beibei, Chongqing 400715, China. .
Wang KX; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Beibei, Chongqing 400715, China. .
Zha XF; State Key Laboratory of Silkworm Genome Biology, Biological Science Research Center, Southwest University, Beibei, Chongqing 400715, China. .; Chongqing Key Laboratory of Sericultural Science, Southwest University, Chongqing 400715, China. .; Chongqing Engineering and Technology Research Center for Novel Silk Materials, Southwest University, Chongqing 400715, China. .
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Source:
International journal of molecular sciences [Int J Mol Sci] 2019 May 13; Vol. 20 (9). Date of Electronic Publication: 2019 May 13.
Publication Type:
Journal Article
MeSH Terms:
Bombyx/*genetics
Insect Proteins/*genetics
RNA Splicing/*genetics
Spodoptera/*genetics
Alternative Splicing/genetics ; Animals ; Calorimetry, Differential Scanning ; Circular Dichroism ; Electrophoretic Mobility Shift Assay ; Exons/genetics ; Female ; Male ; Protein Binding
Academic Journal
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Showing 1-20 from 2323 results

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