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Tytuł :
Two Dimensional Anion Exchange-Size Exclusion Chromatography Combined with Mathematical Modeling for Downstream Processing of Foot and Mouth Disease Vaccine.
Autorzy :
Hossienizadeh SMJ; Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran.
Bagheri M; Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran; Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran.
Alizadeh M; Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran; Department of Plant Breeding & Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51664, Iran.
Rahimi M; Process Simulation and Control Research Laboratory, School of Pharmaceutical and Chemical Engineering, Shahid Beheshti University, Tehran, Iran.
Azimi SM; Foot and mouth Disease Reference Laboratory, Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Kamalzade M; Department of Viral Vaccine Quality Control, Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Es-Haghi A; Department of Physico Chemistry, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), P.O. Box 31975/148 Karaj, Iran.
Ghassempour A; Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C., Evin, Tehran, Iran. Electronic address: .
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Źródło :
Journal of chromatography. A [J Chromatogr A] 2021 Apr 26; Vol. 1643, pp. 462070. Date of Electronic Publication: 2021 Mar 15.
Typ publikacji :
Journal Article
MeSH Terms :
Models, Theoretical*
Chromatography, Gel/*methods
Chromatography, Ion Exchange/*methods
Viral Vaccines/*analysis
Animals ; Cell Line ; Cricetinae ; Dynamic Light Scattering ; Electrophoresis, Polyacrylamide Gel ; Foot-and-Mouth Disease/prevention & control ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Viral Vaccines/isolation & purification ; Virus Inactivation
Czasopismo naukowe
Tytuł :
Peroxidase zymograms obtained by agarose native gel electrophoresis have unmet resolution and completeness.
Autorzy :
Oloketuyi S; Laboratory for Environmental and Life Sciences, University of Nova Gorica, Slovenia.
Annovi G; Laboratory for Environmental and Life Sciences, University of Nova Gorica, Slovenia.
de Marco A; Laboratory for Environmental and Life Sciences, University of Nova Gorica, Slovenia. Electronic address: .
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Źródło :
International journal of biological macromolecules [Int J Biol Macromol] 2020 Aug 01; Vol. 156, pp. 869-873. Date of Electronic Publication: 2020 Apr 11.
Typ publikacji :
Journal Article
MeSH Terms :
Electrophoresis, Agar Gel*/methods
Sepharose*
Peroxidase/*chemistry
Peroxidase/*isolation & purification
Electrophoresis, Polyacrylamide Gel ; Hydrogen-Ion Concentration
Czasopismo naukowe
Tytuł :
Introduction and Perspectives of DNA Electrophoresis.
Autorzy :
Hanada K; Clinical Engineering Research Center, Faculty of Medicine, Oita University, Yufu, Oita, Japan. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2119, pp. 1-13.
Typ publikacji :
Journal Article
MeSH Terms :
DNA*/analysis
DNA*/chemistry
Electrophoresis, Gel, Pulsed-Field*
Electrophoresis, Gel, Two-Dimensional*
DNA Breaks ; DNA Replication ; Electrophoresis, Agar Gel
Czasopismo naukowe
Tytuł :
Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage.
Autorzy :
Kolesar JE; Department of Animal Biology, University of Pennsylvania, Philadelphia, PA, USA.
Kaufman BA; Division of Cardiology, Department of Medicine, Center for Metabolism and Mitochondrial Medicine and the Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2119, pp. 25-42.
Typ publikacji :
Journal Article
MeSH Terms :
DNA Replication*
DNA, Mitochondrial*/analysis
DNA, Mitochondrial*/metabolism
Electrophoresis, Gel, Two-Dimensional*
Mitochondria/*metabolism
Animals ; Electrophoresis, Agar Gel ; Humans
Czasopismo naukowe
Tytuł :
Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers.
Autorzy :
Gibson EG; Department of Pharmacology, Vanderbilt University School of Medicine, Nashville, TN, USA.
Oviatt AA; Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA.
Osheroff N; Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA. .; Department of Medicine (Hematology/Oncology), Vanderbilt University School of Medicine, Nashville, TN, USA. .; VA Tennessee Valley Healthcare System, Nashville, TN, USA. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2119, pp. 15-24.
Typ publikacji :
Journal Article
MeSH Terms :
Electrophoresis, Gel, Two-Dimensional*
DNA Topoisomerases/*analysis
DNA, Superhelical/*analysis
Electrophoresis, Agar Gel
Czasopismo naukowe
Tytuł :
Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures.
Autorzy :
Mettrick KA; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
Weaver GM; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.
Grainge I; School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2119, pp. 61-72.
Typ publikacji :
Journal Article
MeSH Terms :
Blotting, Southern*
DNA Replication*
DNA, Bacterial*/analysis
DNA, Bacterial*/genetics
DNA, Bacterial*/metabolism
Electrophoresis, Gel, Two-Dimensional*
Escherichia coli*/genetics
Escherichia coli*/metabolism
Electrophoresis, Agar Gel
Czasopismo naukowe
Tytuł :
Application of Two-Dimensional Difference Gel Electrophoresis in Identification of Factors Responsible for Virulence of Staphylococcus aureus.
Autorzy :
Bonar E; Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
Chlebicka K; Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
Dubin G; Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.; Department of Microbiology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland.
Wladyka B; Department of Analytical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland. .
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2069, pp. 139-154.
Typ publikacji :
Journal Article; Research Support, Non-U.S. Gov't
MeSH Terms :
Electrophoresis, Gel, Two-Dimensional*
Proteomics*
Staphylococcal Infections/*metabolism
Staphylococcus aureus/*metabolism
Staphylococcus aureus/*pathogenicity
Virulence Factors/*biosynthesis
Animals ; Humans ; Two-Dimensional Difference Gel Electrophoresis
Czasopismo naukowe
Tytuł :
A Fluorescent Probe for the Specific Staining of Cysteine Containing Proteins and Thioredoxin Reductase in SDS-PAGE.
Autorzy :
Liu Y; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Yu Y; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Meng Q; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Jia X; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Zhu J; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Tang C; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Zhao Q; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Feng X; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Zhang J; State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.; Scientific Observing and Experiment Station of Animal Genetic Resources and Nutrition in North China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
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Źródło :
Biosensors [Biosensors (Basel)] 2021 Apr 23; Vol. 11 (5). Date of Electronic Publication: 2021 Apr 23.
Typ publikacji :
Journal Article
MeSH Terms :
Electrophoresis, Polyacrylamide Gel*
Cysteine/*chemistry
Thioredoxin-Disulfide Reductase/*chemistry
Animals ; Biosensing Techniques ; Fluorescence ; Fluorescent Dyes ; Oxidation-Reduction ; Sheep ; Staining and Labeling
Czasopismo naukowe
Tytuł :
Sample Preparation of Secreted Mammalian Host Cell Proteins and Their Characterization by Two-Dimensional Electrophoresis and Western Blotting.
Autorzy :
Posch A; Bio-Rad Laboratories GmbH, Munich, Germany. .
Kollmann F; Bio-Rad Laboratories GmbH, Munich, Germany.
Berkelman T; Bio-Rad Laboratories, Inc., Hercules, CA, USA.
Dreskin E; Bio-Rad Laboratories, Inc., Hercules, CA, USA.
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Źródło :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2261, pp. 507-524.
Typ publikacji :
Journal Article
MeSH Terms :
Blotting, Western*
Electrophoresis, Gel, Two-Dimensional*
Electrophoresis, Polyacrylamide Gel*
Proteome*
Proteomics*
Proteins/*analysis
Analytic Sample Preparation Methods ; Animals ; CHO Cells ; Cricetulus ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Antibody Technique ; HEK293 Cells ; Humans ; Secretory Pathway ; Workflow
Czasopismo naukowe

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